Adult ; Biomarkers, Tumor ; metabolism ; Epididymis ; metabolism ; pathology ; surgery ; Humans ; Inhibins ; metabolism ; Leydig Cell Tumor ; metabolism ; pathology ; surgery ; Male ; Testicular Neoplasms ; metabolism ; pathology ; surgery ; Vimentin ; metabolism

Objective To explore the incidence rate, total incidence numbers and possible impacting factors of non-motor symptoms (NMSs) in Parkinson's disease (PD). Methods The NMS questionnaire (NMS Quest) was used to investigate 131 out-patients diagnosed with PD, and the prevalence of each item, the total NMS numbers and their relationships with clinical features were assessed. Results All of the patients, who were distributed in every stage of PD, had NMSs and each case with 11 items on average. Autonomic symptoms such as nocturia and constipation were the most frequent ones. The patients over 65 versus less than 65 years old had a higher prevalence in a number of items such as olfactory disturbance, dysphagia and constipation. At the same time, the rigidity subtype patients had a higher prevalence in depression items than the tremor subtype ones.The total number of NMS was positively correlated with course of disease, age, UPDRS score and Hoehn & Yahr (H&Y) stage. Conclusions NMSs are common among PD patients. While the prevalence of each item may vary with different clinical features, the total number of NMS is increased with the severity of PD. More attention should be paid to the diagnosis and rational treatment of the NMSs.

[Objective]To discuss the best acupuncture treatment to acute facial neuritis.[Method] Compare the routine acupuncture method with plum needle acupuncturing Wanggu acupoint for bleeding.[Result] After 3 courses,the cure rate of the treatment group was 66.7%,and 33.3% for the control one;by comparison,the difference was of marked meaning(P

Objective To explore the mechnism of toxicity induced by cisplatin on renal proximal tubular cells(RPTC) line and anti-apoptosis mechanism of BCL-2 with transfection of different mutant BCL-2 in vitro.MethodsRPTC was translocated with different mutants BCL-2(s).Cell apoptosis induced by cisplatin on RPTC was analyzed with confocal and flurencent microscope.The cell apoptosis was measured with Hoechst33258 after treatment with cisplatin.Results Different mutant BCL-2(BCL-acta,BCL-cb5)were translocated on mitochondrial and Endoplasmic Reticulum(ER) respectively.BCL-acta protected RPTC from apoptosis induced by cisplatin more easily than BCL-cb5 group in a time-dependant manner(P

Objectives:To investigate the expression of regulators survivin and Ki 67 in rhabdomyosarcoma(RMS), and to evaluate their relationship with clinicopathological features. Methods: Immunohistochemical technique(S P) and image analysis were used to detect the expression of regulators survivin and Ki 67 in 43 cases of RMS and 10 normal skeletal muscles. Results: Expression of survivin was detected in 86% of the RMS, with higher levels in RMS than in normal skeletal muscles ( P

OBJECTIVE: To develop a method for determining the concentration of lamivudine in placental perfusate by employing ultra fast liquid chromatography with tandem mass spectrometry (UFLC-MS/MS). METHODS: Famotidine was used as the internal standard. After protein precipitation with acetonitrile, the chromatography was performed on a Shim-pack XR-ODS II C18 column (2.0 mm × 75 mm, 2.2 μm) with mobile phase consisting of methanol and 10 mmol · L-1 ammonium acetate, gradient elution was used, and the flow rate was 0.4 mL · min-1. Tandem mass spectrometric detection was conducted by using multiple reaction monitoring (MRM) in positive ionization mode with an electrospray ionization interface. RESULTS: The calibration curve of lamivudine was linear in the range of 1-2500 ng · mL-1. The intra- and inter-batch RSDs were less than 10%. The method recovery was more than 72%. CONCLUSION: The method is rapid and accurate, which is suitable for the determination of lamivudine in placental perfusate.

Objective To evaluate the role of arthroscopy in diagnosis and treatment of osteoarthritis of the knee.Methods The data of 108 patients (118 knees) with osteoarthritis of the knee and received arthroscopic surgery were analyzed retrospectively including sign, X-ray and MRI examinations of knee, the average stage in hospital, diagnoses before and after operation, and prognosis.Results Arthroscopic surgery was of benefit to diagnosis and treatment of osteoarthritis of knee. The average stage in hospital was 11±7 days. The following-up of 98 patients showed that 82 patients were excellent, 16 patients good and only 1 patients not up to standard.Conclusion Arthroscopic surgery has following advantages such as less invasive surgery, shorter stage in hospital, good curative effect in the near future.

Academic Dissertations as Topic ; Bibliometrics ; China ; Health Systems Agencies ; Occupational Diseases ; prevention & control ; Publishing

BACKGROUND:Heart valve surgery combined with coronary artery bypass grafting has been gradual y used for treatment of coronary heart disease combined with cardiac valve disease.
OBJECTIVE:To retrospectively summarize the experience of combined coronary artery bypass grafting and valvular procedure.
METHODS:Total y 51 patients who underwent combined heart valve surgery and coronary artery bypass grafting were retrospectively analyzed, including 10 cases with aortic valve replacement, 14 cases with mitral valve replacement, eight cases with mitral valve replacement combined with tricuspid annuloplasty, four cases with aortic valve and mitral valve replacement, three cases with aortic valve and mitral valve replacement combined with tricuspid annuloplasty, seven cases with mitral valvuloplasty, and five cases with mitral valvuloplasty combined with tricuspid annuloplasty.
RESULTS AND CONCLUSION:Biovalve replacement was performed in nine patients, mechanical valves replacement in 31 cases, mitral valvuloplasty in 11 cases and tricuspid annuloplasty in 16 cases. There were total y 109 bypass graft vessels, and the average number of coronary artery bypass grafts was (1.92±0.73) branches. Four cases died within 30 days postoperatively, and 47 patients were successful y discharged from the hospital. Forty-five of 47 discharging patients were fol owed for 3-48 months. One case died of cerebral infarction within 6 months postoperatively, and another case died of cardiac dysfunction over 1 year after operation. The heart function of 45 survival patients was significantly improved. The comprehensive analysis showed that improving the heart function preoperatively, strengthening myocardial protection, shortening operation and myocardial ischemia time, and complete revascularization are the key factors for successful operation.

Objective To design a kind of aseptic drainage device with safe practice and accurate measurement for clinical nurses. Methods The experiment group included 63 cases using drainage device with accurate measurement,and control group included 63 cases using disposal drainage bag with scale of visual measurement.The accuracy of drainage liquid measurement was compared between two groups,and bacterial culture was made for the drainage liquid of two groups.Results The measurement error of experiment group and control group was(3.31±1.8)mL and(56.0±5.8)mL,respectively.The difference was significant(t=-4.593,P<0.01).The bacterial culture results showed no obvious difference between two groups(positive rate were 15.9%vs 17.5%,χ2=0.057,P>0.05). Conclusion The accurately measured aseptic drainage device is convenient for nurses to practice in clinics,and its application can reduce workload of nurses and decrease the occurrence of pollution.