Objective To observe the effect of X-ray radiation on NF-κB-HIF-1α pathway activation in three malignant lymphoma and to explore the mechanism of radioresistance mediated by this pathway.Methods Expression levels of p-P65,IKK and HIF-1α in Namalwa,Ramos and Raji cells were determined by Western blot 1,4,10 and 20 h after 5 Gy X-ray irradiation.The effect of QNZ on HIF-1α expression was also observed.Results Expression level of IKK protein was up-regulated 4 h after irradiation in the 3 lymphoma cell strains( t = 8.01,5.14,5.42,P ＜ 0.01 ),followed by up-regulation of p-P65 protein expression level at 10 h and HIF-1α at 10-20 h after X-ray irradiation (t = 11.25,17.43,22.09,P ＜ 0.01 ).Pretreatment of QNZ 24 h before X-ray irradiation significantly reduced the upregulation of HIF-1α protein expression induced by simple ion radiation ( t = 18.69,19.35,12.26,P ＜0.01 ).Conclusion NF-κB - HIF-1α pathway was activated in human lymphoma cells after ion radiation and may be involved in the mechanism of radioresistance.

Objective: To analyze the relationship of the expression level of estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor-2 (HER-2, c-erbB-2) of breast invasive ductal carcinoma (IDC) with ultrasonographic characteristics. Methods: Totally 104 patients with IDCs confirmed pathologically were involved in this study. ER, PR and c-erbB-2 expression in the IDC specimens was determined by immunohistochemical staining technique. The correlation between the ultrasonographic features and the positive expression of ER, PR or c-erbB-2 was analyzed. Results: The positive expression rate of ER and PR in the group with tumor spiculation sign and posterior acoustic attenuation was higher than that in the group without. The positive expression rate of ER differed significantly (P < 0.05) while that of PR did not (P>0.05). The over-expression rate of c-erbB-2 in the group of microcalcification, sufficient blood flow and axillary lymph node metastasis was higher than that in the group of non-microcalcification, deficient blood flow or without axillary lymph node metastasis (P < 0.05). The expression of ER, PR and c-erbB-2 was not related to the size of tumor (P > 0.05). Conclusion: The expression of ER and c-erbB-2 is closely related to the ultrasonographic characteristics of IDC, which may, to some extent, reflect the expression level of ER and c-erbB-2.

Objective: To analyze the relationship of the expression level of estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor-2 (HER-2, c-erbB-2) of breast invasive ductal carcinoma (IDC) with ultrasonographic characteristics. Methods: Totally 104 patients with IDCs confirmed pathologically were involved in this study. ER, PR and c-erbB-2 expression in the IDC specimens was determined by immunohistochemical staining technique. The correlation between the ultrasonographic features and the positive expression of ER, PR or c-erbB-2 was analyzed. Results: The positive expression rate of ER and PR in the group with tumor spiculation sign and posterior acoustic attenuation was higher than that in the group without. The positive expression rate of ER differed significantly (P < 0.05) while that of PR did not (P>0.05). The over-expression rate of c-erbB-2 in the group of microcalcification, sufficient blood flow and axillary lymph node metastasis was higher than that in the group of non-microcalcification, deficient blood flow or without axillary lymph node metastasis (P < 0.05). The expression of ER, PR and c-erbB-2 was not related to the size of tumor (P > 0.05). Conclusion: The expression of ER and c-erbB-2 is closely related to the ultrasonographic characteristics of IDC, which may, to some extent, reflect the expression level of ER and c-erbB-2.

Objective To investigate the mechanism of xCT on tumor metastasis in breast cancer cell MDA-MB-231. Methods Wound scratch assay and Transwell assay were performed to evaluate the effect of disruption and knockdown of xCT on cell migration and cell invasion in breast cancer cell MDA-MB-231 .Western Blot and RT-PCR were used to detect the expression levels of autophagy and EMT related markers in breast cancer cell MDA-MB-231 after treatment with sulfasalazine (SASP), an inhibitor of xCT activity and SLC7A11-RNAi.Results Both the scratch assay and the transwell migration assay showed that inhibition of xCT reduced the motility of MDA-MB-231 .The expression level of autophagy related protein LC3-Ⅱ/LC3-Ⅰwas elevated, the protein level of transcription factor Snail was down-regulated, while the mRNA level of Snail did not change in xCT inhibited MDA-MB-231 cells compared with MDA-MB-231 cells.Epithelial marker E-cadherin was up-regulated but mesenchymal marker Vimentin was down-regulated when xCT was deficient.Con-clusion Our current studies show that xCT is an endogenous regulator of tumor growth and metastasis in MDA -MB-231 and the expression level of xCT determines the phenotypes of MDA-MB-231 cells in invasion and migration in vitro.Inhibition of xCT can activate autophagy , induce the degradation of Snail ,and attenuate the EMT process in highly metastatic MDA-MB-231 cells.

Objective　To establish the method of alloantigen-specific T lymphocyte clones. Methods　PBMC of the recipient was stimulated by donor splenocytes for twice and the stimulated T lymphocytes were cloned by limited dilution. Results　Three clones of T lymphocytes were obtained. By analysis of immunohistochemistry, two of the three clones were CD3+CD4-CD8+, another one was CD3+CD4-CD8-. The latter was analyzed by flowcytometry and CD3+ was 98.87%, CD4- 97.05%, CD8- 97.14%. Antigen specificity of the cloned T lymphocytes was determined by stimulation of donor splenocytes and non-related individual splenocytes. When stimulated by donor splenocytes, the cloned T lymphocytes proliferated and did not show proliferative role when stimulated by non-related individual splenocytes. Conclusion　Our method of cloning alloantigen-specific T lymphocytes is successful.

Objective To explore the relationship of the mean color vessels density (MCVD) and pathologic microvessel density(MVD) in rectal cancers and their relation with T stags and lymph node metastasis.Methods MCVD were caculated preoperationly with transrectal color power angiography(TRCPA).After operation MVD was assessed immunohistochemically using anti-CD105 monoclonal antibody.The relationships within MCVD, MVD and T stages and lymph node metastasis were analysed.Results There were positive correlation between MCVD and MVD (r=0.763, P＜0.01) in rectal cancer.There were significant difference of MCVD and MVD in both the depth of carcinoma invasion and metastasis of lymphatic nodes( P＜0.05).Conclusions MCVD can display features of the blood supply and distribution of pathologic microvessels and reflect the development of rectal cancers.MCVD is a credible index to choose available treatment and to evaluate prognosis before operation of rectal cancer.

Adipose Tissue ; cytology ; Heart ; physiology ; Humans ; Regeneration ; Stem Cells ; cytology

Objective To understand multidrug-resistant organism (MDRO) healthcare-associated infection(HAI) in patients with malignant tumor, so as to provide basis for making HAI prevention and control measures.Methods Targeted surveillance method was used to survey MDRO HAI in patients with malignant tumor in a hospital from January 1 to December 31, 2014, WHONET 5.3 software was used to analyze the data.Results A total of 54 056 patients with malignant tumor were surveyed, HAI occurred in 3 542 (6.55%) patients, 847(23.91%)of which were MDRO HAI.Most patients(54.55%) with MDRO HAI were >60 years old.A total of 2 606 bacterial strains were isolated from patients with HAI, 847 (32.50%) of which were MDROs, and most were extended-spectrum beta-lactamases (ESBLs)-producing Escherichia coli (n=459, 54.19%).The major site of MDRO HAI was lower respiratory tract (47.34%).The top 3 departments of detection of MDROs were departments of thoracic surgery(17.12%), colorectal and anal surgery(9.92%), and general surgery (8.26%).Conclusion Incidence of MDRO HAI is higher in patients with malignant tumor, surveillance of high risk population and monitoring of antimicrobial resistance of pathogens should be strengthened, so as to reduce the occurrence of HAI.

Objective To Study the relation between analgesic action of alcohol extraction of Natrix tigrina Lateralis and central nervous system. Methods Manifold ache models of experimental nerve centre were established to observe the analgesic action and degree of alcohol extraction of Natrix tigrina Lateralis to the ache models. Results Alcohol extraction of Natrix tigrina Lateralis had analgesic action to all ache models of nerve centre, and the high-dose group was obviously. Conclusions Analgesic action of alcohol extraction of Natrix tigrina Lateralis is related to effecting on central nerves system, which effects antinociceptive in central nervous.

BACKGROUND: Hippocampus is one of an important brain areas related with memory,and plays a critical role in associative memory function.Hippocampal CA3 is one of the most important regions to form associative memory.CA3 is functionally divided into autoassociative and heteroassociative memories,and memory formation and retrieval require the development of detailed models of hippocampal function.OBJECTIVE: To establish a detailed model of hippocampal CA3 function according to CA3 structure.METHODS: The model was a three-layered Hopfield-like neural network and was constituted by 280 Izhikevich artificial neurons,and is modulated by Hebbian rules.The model was simulated using MATLAB under the condition of adding the Gaussian white noise to its input.In the simulation,memories were represented by synchronous firing sequences.RESULTS AND CONCLUSION: The simulating results show that the third layer of model had heteroassociative memory function;the first and the second layer of the model could implement autoassociative memory.The model implemented well the memory functions of three subregions of hippecampal CA3.But it is impossible to understand the functions and dynamics of a real biological neural network by constructing a simple model.The model proposed has 280 neurons,which are far less than the real number of neurons.It suggests that there is a big gap between the properties of the model and a real biological neural network of CA3.