Objective To investigate the effects of dexmedetomidine on the outcome in rats with sepsis. Methods Male SD rats, aged 10-14 weeks, weighing 260-390 g, were used in this study. Sepsis was induced by cecal ligation and puncture (CLP). Ninety rats of successful sepsis model were randomly divided into 3 groups ( n = 30 each) : control group (group C), midasolam group (group M) and dexmedetomidine group (group D). In group C, M and D, normal saline at a rate of 1 ml/h, midazolam at a rate of 0.6 mg·kg-1·h-1 and dexmedetomidine at a rate of 5 μg·kg-1·h-1 were infused iv for 8 h after operation respectively. Ten rats of each group were selected for observation of the survival condition during 24 h after operation. Another 10 rats of each group were selected and blood samples were taken from carotid artery before operation and at 2, 4 and 5 h after operation for measurement of plasma concentrations of TNF-α and IL-6 by ELISA. The remaining 10 rats of each group were selected at 8 h after operation for determination of the renal function. The rats still alive after the determination of cytokines and renal function were killed and spleen tissues were taken for determination of the expression of caspase-3 and ubiquitin by Western blot. Results Compared with group C, the plasma concentration of TNF-α and fractional excretion of sodium (FENa+) were significantly decreased, caspase-3 expression in spleen tissues was down-regulated and abiquitin expression in spleen tissues was up-regulated in group M and D ( P < 0.05), while there were no significant differences in plasma concentration of IL-6 and creatinine clearance rate (Ccr) at 8 h after operation between group M and C and between group D and C ( P > 0.05). The plasma concentration of TNF-α was significantly lower in group D than in group M ( P < 0.05), while there were no significant differences in plasma concentration of IL-6, FE Na+ , Ccr and expression of caspase-3 and ubiquitin in splen tissues between group D and M ( P>0.05). The survival rates during 24 h after operation were 10%, 80% and 90% in group C, M and D respectively. The survival rates during 24 h after operation were signifrcantly higher in group M and D than in group C ( P > 0.05). Conclusion Dexmedetomidine can raise the survival rate during sepsis.

The overexpression of P-glycoprotein(P-gp)and its coding gene mdrl is regarded as the classic mechanism of muhidrug resistance(MDR).Recent studies find that pregnane X receptor(PXR)can mediate the expression of P-gp.It is confirmed that PXR can also inhibit apoptosis of tumor cells.Therefore,preventing the activation of PXR specifically may be a new method to prevent MDR.

AIM:To investigate the effects of a novel thiazolidinedione ANY2 on glycometabolism in HepG2 cells.METHODS:HepG2 cells are exposed to high concentrations of insulin for 24 h to build the model of insulin-resistance(IR-HepG2).The effects of ANY2,including glucose consumption(GC),gluconeogenesis and glucolysis were tested in this model.RESULTS:ANY2 could dose-dependently enhance GC in IR-HepG2 cells at high,medium and low levels of glucose.Compared with Modle Group,ANY2 increased IR-HepG2 cells glycogen synthesis significantly only in high dose group.It inhibited gluconeogenesis from lactic acid,but increased the production of lactic acid and the activity of pyruvate kinase(PK)in glucolysis.CONCLUSION:Compound ANY2 has remarkable effects on improving glycometabolism in IR-HepG2 cells.

Objective To investigate the refining and concentration of the Banlangen aqueous extractives using membrane separation technology. Methods The membrane separation process for refining and concentration Banlangen aqueous extractives was optimized by the quality of products and membrane operation parameters, and was tested through medium-scale trials. Results The selected G2+GM+RO2 membrane process was suitable. The inherent quality of the products obtained by membrane separation process was better than that obtained by traditional process. Conclusion G2+GM+RO2 membrane process is suitable to refine and concentrate Banlangen aqueous extractives, and it has technical conditions for industrial scale trials.

Objective To compare Th1/Th2 drift situation in patients with lymphoma with that in patients with solid tumors, and establish the effective immune function detectable criterion of lymphoma in biological treatment process. Methods The whole blood samples of 10 patients with lymphoma, 202 patients with solid tumors including 36 patients with upper digestive tract cancer, 64 colorectal cancer, 43 lung cancer and 20 the other malignancies, and 30 healthy persons as controls were collected. Stimulation agent was used to stimulate the cells in order to increase cell factor expression and fluorescent labeled specific anti-cytokine monoclonal antibody was used to bind with specific antigen. The expression of specific cytokines was detected by flow cytometry. Results Positive percentages of IFN- γ and IL-4 and ratio of IFN- γ /IL-4 in CD+4 T cells of human peripheral blood had statistically differences between in patients with lymphoma and controls (P < 0.05). The ratio of Thl/Th2 (CD+4 intracellular cytokine INF-α/IL-4) in patients with lymphoma was lower than that in patients with upper digestive tract cancers (esophagus and stomach cancers) (P = 0.023), however, had no statistical differences with that in patients with other solid tumors (colorectal, lung, kidney, breast and other tumors), but had a downward trend. Conclusion Immune functions in patients with lymphoma are lower than those in patients with solid tumors and immune treatment is a necessary to adjuvant chemotherapy. The ratio of Th1/Th2 (INF- α/IL-4) is expected to become effective detection criterion.

PURPOSE To investigate the effects of all trans retinoic acid(ATRA) on the growth of gastric cancer cells and explore the mechanism relevant to enzyme.METHODS With MTT method the growth inhibition of gastric cancer cells was measured;the activity of lactate dehydrogenase(LDH),alkaline phosphatase(ALP) and ? glucuronidase(? G) was measured with metaplate;The activity of succinate dehydrogenase(SDH) was assayed with cytochemical method.RESULTS The growth of MGc80 3, but not MKN 45, was inhibited,which is dependent on the concentration of ATRA.The activity of LDH,ALP and ? G of Mgc80 3 was suppressed and the activity of SDH of Mgc80 3 was enhanced.But all the four enzymes′ activity of MKN 45 were not obviously changed except that introcelluar ? G activity was inhibited.CONCLUSIONS The sensitivity of gastric cancer cells to ATRA is different,which is closely related with the change of enzyme activity.

Objective To analyze effect of three level nursing quality control management model on the quality of the nursing service in the department of general surgery.Methods Analysis in the implementation of nurs-ing quality control before and after nursing quality and the improvement of the examination results of nurses situation in general surgery department of our hospital were compared between the 2012 -2013 without the implementation of three level quality control mode and 2013 -2014 of establishment of the three level nursing quality control manage-ment mode,which was set the nursing quality monitoring nurses,quality monitoring group leader and quality monito-ring nurses in three level nursing quality control system.Results After the implementation of quality control,the quality of basic nursing score[(94.02 ±2.01)points],ward management quality score[(94.13 ±3.04)points],first aid management quality score[(96.22 ±4.06)points]and disinfection and isolation,and the quality score[(95.13 ± 3.04)points]and quality control of basic nursing quality score[(8.121 ±3.31 )points],ward management quality score[(81.35 ±2.34)points],emergency management quality score [(91.42 ±4.36 )points]and disinfection [(81.74 ±3.44)points]degrees and isolation quality scores were significantly higher than those of before three level quality control nursing management model(t =33.07,33.32,8.06,29.17,all P <0.05).Nursing quality control of nursing personnel the technical operation of the average score [(96.35 ±1.03)points],theory test average score [(95.73 ±0.98)points],average and quality control of nursing personnel and technical operation [(89.42 ± 2.49)points],theory test average score[(90.36 ±1.79)points]were obviously higher.The results had statistical significance (t =25.72,26.32,all P <0.05).Conclusion Three level nursing quality control management model established in the general surgery department had remarkable effect on the quality of the nursing service,which is safe and reliable,and improving the quality of nursing.

Objective To investigate the effect of continuous quality improvement nursing on grasping disease knowledge and satisfaction of patients with thyroid tumors.Methods 86 cases of patients with thyroid cancer were enrolled.They were devided into the observation group(42 cases)and the control group(46 cases)according to the date of admission.Patients in control group were treated with routine care,while patients in the observation group were treated with continuous quality improvement nursing on the basis of the control group.The effects of nursing in the two groups were compared and analysized.Results After intervention,the scores of cognitive thyroid tumors,daily preventive health care,thyroid cancer surgery and surgical considerations in the observation group were higher than those in the control group,theses differences were statistically significant between the two groups (t =9.806,10.271, 9.924,12.053,all P <0.05).The rate of satisfaction in the observation group was significantly higther than that in the control group(92.86%/75.00%),theses difference was statistically significant between the two groups (χ2 =9.025,P <0.05).Conclusion Continuous quality improvement nursing can effectively improve the diseases -related knowledge in patients with thyroid benign tumor,which contributes to the smooth operation and improves surgical results.

Female ; Humans ; Menstruation ; physiology ; Menstruation Disturbances ; epidemiology ; etiology ; Occupational Health ; Stress, Psychological ; complications ; epidemiology ; physiopathology

Objective To detect the expression of serum soluble E?cadherin(sEC)before and after operation in breast cancer patients,and to ex?plore its clinical significance. Methods The levels of sEC in 42 patients with breast cancer and 18 patients with benign breast diseases were tested by enzyme?linked immunosorbent assay(ELISA)before and after operation as well as in 30 healthy controls. Its correlation with clinical and patho?logical characteristics was analyzed. Results The levels of sEC in breast cancer patients before operation were significantly higher than patients with benign breast diseases and controls,which declined significantly after operation. Moreover,the levels of sEC in those with high clinical grading and lymphatic metastasis were also higher than in the ones with low grading and negative lymphatic metastasis(P<0.05). Conclusion The levels of sEC in breast cancer patients before operation are specific and closely related with pathological grade and lymphatic metastasis,which may be a pre?dictive factor for prognosis of breast cancer.