Objective To investigate the effects of dexmedetomidine on the outcome in rats with sepsis. Methods Male SD rats, aged 10-14 weeks, weighing 260-390 g, were used in this study. Sepsis was induced by cecal ligation and puncture (CLP). Ninety rats of successful sepsis model were randomly divided into 3 groups ( n = 30 each) : control group (group C), midasolam group (group M) and dexmedetomidine group (group D). In group C, M and D, normal saline at a rate of 1 ml/h, midazolam at a rate of 0.6 mg·kg-1·h-1 and dexmedetomidine at a rate of 5 μg·kg-1·h-1 were infused iv for 8 h after operation respectively. Ten rats of each group were selected for observation of the survival condition during 24 h after operation. Another 10 rats of each group were selected and blood samples were taken from carotid artery before operation and at 2, 4 and 5 h after operation for measurement of plasma concentrations of TNF-α and IL-6 by ELISA. The remaining 10 rats of each group were selected at 8 h after operation for determination of the renal function. The rats still alive after the determination of cytokines and renal function were killed and spleen tissues were taken for determination of the expression of caspase-3 and ubiquitin by Western blot. Results Compared with group C, the plasma concentration of TNF-α and fractional excretion of sodium (FENa+) were significantly decreased, caspase-3 expression in spleen tissues was down-regulated and abiquitin expression in spleen tissues was up-regulated in group M and D ( P < 0.05), while there were no significant differences in plasma concentration of IL-6 and creatinine clearance rate (Ccr) at 8 h after operation between group M and C and between group D and C ( P > 0.05). The plasma concentration of TNF-α was significantly lower in group D than in group M ( P < 0.05), while there were no significant differences in plasma concentration of IL-6, FE Na+ , Ccr and expression of caspase-3 and ubiquitin in splen tissues between group D and M ( P>0.05). The survival rates during 24 h after operation were 10%, 80% and 90% in group C, M and D respectively. The survival rates during 24 h after operation were signifrcantly higher in group M and D than in group C ( P > 0.05). Conclusion Dexmedetomidine can raise the survival rate during sepsis.

Objective To investigate the role of anti-herpes ximplex virus (HSV)-1 IgM secreting cells detection assay in early diagnosis of herpes simplex encephalitis. Methods Twenty-three herpes simplex encephalitis cases and 40 control cases were included in this study. Anti-HSV-1 IgM secreting cells and anti-HSV-1 IgM were retrospectively tested in the patients' cerebrospinal fluid by enzyme-linked immunosorbent spot (ELISPOT) and enzyme-linked immunosorbent assay (ELISA), respectively. The data analysis was performed by using Fisher Exact Test. Results Using ELISPOT method for detection of 9 HSV-1 encephalitis patients' and 16 clinical control cases anti-HSV-1 IgM secreting cells within two weeks after disease onset, the sensitivity of ELISPOT for detecting anti HSV-1 IgM secreting cells in the cerebrospinal fluid was 88.9% (8/9) and the specificity was 93.8%(15/16). On the other hand, the sensitivity of ELISA for detecting anti-HSV-1 IgM in ccrebrospinal fluid was 16.6% (2/12) and the specificity was 88.2% (15/17) when using ELISA method for detection of 12 HSV-1 encephalitis patients' and 17 clinical control cases's anti-HSV-1 IgM secreting cells. The sensitivities of the two methods were statistically different (P<0.01). Conclusion Compared to ELISA, ELISPOT for detecting the anti-HSV-1 IgM secreting cells in cerehrospinal fluid is a more sensitive method for early diagnosing herpes simplex encephalitis.

AIM:To investigate the effects of a novel thiazolidinedione ANY2 on glycometabolism in HepG2 cells.METHODS:HepG2 cells are exposed to high concentrations of insulin for 24 h to build the model of insulin-resistance(IR-HepG2).The effects of ANY2,including glucose consumption(GC),gluconeogenesis and glucolysis were tested in this model.RESULTS:ANY2 could dose-dependently enhance GC in IR-HepG2 cells at high,medium and low levels of glucose.Compared with Modle Group,ANY2 increased IR-HepG2 cells glycogen synthesis significantly only in high dose group.It inhibited gluconeogenesis from lactic acid,but increased the production of lactic acid and the activity of pyruvate kinase(PK)in glucolysis.CONCLUSION:Compound ANY2 has remarkable effects on improving glycometabolism in IR-HepG2 cells.

Objective To analyze effect of three level nursing quality control management model on the quality of the nursing service in the department of general surgery.Methods Analysis in the implementation of nurs-ing quality control before and after nursing quality and the improvement of the examination results of nurses situation in general surgery department of our hospital were compared between the 2012 -2013 without the implementation of three level quality control mode and 2013 -2014 of establishment of the three level nursing quality control manage-ment mode,which was set the nursing quality monitoring nurses,quality monitoring group leader and quality monito-ring nurses in three level nursing quality control system.Results After the implementation of quality control,the quality of basic nursing score[(94.02 ±2.01)points],ward management quality score[(94.13 ±3.04)points],first aid management quality score[(96.22 ±4.06)points]and disinfection and isolation,and the quality score[(95.13 ± 3.04)points]and quality control of basic nursing quality score[(8.121 ±3.31 )points],ward management quality score[(81.35 ±2.34)points],emergency management quality score [(91.42 ±4.36 )points]and disinfection [(81.74 ±3.44)points]degrees and isolation quality scores were significantly higher than those of before three level quality control nursing management model(t =33.07,33.32,8.06,29.17,all P <0.05).Nursing quality control of nursing personnel the technical operation of the average score [(96.35 ±1.03)points],theory test average score [(95.73 ±0.98)points],average and quality control of nursing personnel and technical operation [(89.42 ± 2.49)points],theory test average score[(90.36 ±1.79)points]were obviously higher.The results had statistical significance (t =25.72,26.32,all P <0.05).Conclusion Three level nursing quality control management model established in the general surgery department had remarkable effect on the quality of the nursing service,which is safe and reliable,and improving the quality of nursing.

Objective To investigate the effect of continuous quality improvement nursing on grasping disease knowledge and satisfaction of patients with thyroid tumors.Methods 86 cases of patients with thyroid cancer were enrolled.They were devided into the observation group(42 cases)and the control group(46 cases)according to the date of admission.Patients in control group were treated with routine care,while patients in the observation group were treated with continuous quality improvement nursing on the basis of the control group.The effects of nursing in the two groups were compared and analysized.Results After intervention,the scores of cognitive thyroid tumors,daily preventive health care,thyroid cancer surgery and surgical considerations in the observation group were higher than those in the control group,theses differences were statistically significant between the two groups (t =9.806,10.271, 9.924,12.053,all P <0.05).The rate of satisfaction in the observation group was significantly higther than that in the control group(92.86%/75.00%),theses difference was statistically significant between the two groups (χ2 =9.025,P <0.05).Conclusion Continuous quality improvement nursing can effectively improve the diseases -related knowledge in patients with thyroid benign tumor,which contributes to the smooth operation and improves surgical results.

Objective To investigate the effect of specialized human tel omerase antisense oligodeoxyribonucleotides (AS-ODN) on the growth inhibition o f well, moderate and poor differentiated gastric cancer cell lines, and to explore its inhibitory mechanism and the correlation between the inhibition ratio of gastric cancer cells and differentiation of the tumor cells. Methods Under the given circumstances, three distinct differentiated gastric cancer cell lines were treated with AS-ODN. The telomerase a ctivities were measured by modified telomeric repeat amplification protocal assa y. The cell viability was detected by Trypan blue test, and the cell apoptosis was determined by cell morphological observation under light and electromicroscope, flow cytometry an d TUNEL assay. Results The telomerase activity and cell growth were apparently suppressed in MK N45 and SGC7901 cells, under defined concentrations of AS-ODN. Whilst, in MKN28 cells, only telomerase activity was suppressed at same concentration . There were no obvious changes in non-antisense oligomers treated group. The apoptotic features of MKN45 and SGC7901 were noticed by microscopic observa tion, TUNEL assay, after three distinct gastric cancer cell lines being continuo usly exposed to 10 ?mol/L AS-ODN for 96 h. Furthermore, the flow cytometric analy sis verified that the average apoptotic rate of MKN45 and SGC7901 was 44.75% and 33.56% respectively, but there were no obvious changes in non-antisense oli gomers treated group (P

Objective To observe the therapeutic effect of treating primary dysmenorrhea with cold coagulation and blood stasis with warming-necdle. Methods 60 cases of primary dysmenorrhea with cold cougulation and blood stasis,which were treated during 2007 to 2008, were randomly divided into an acupuncture group and a medication group. The acupuncture group was treated with acupuncture at Ciliao and Sanyinjiao, while the medication group was treated with indomethacin. Results The total effective rate was 100% in the acupuncture group, and 93.3% in the medication group. The effects of the two groups were significantly different (P＜0.05). Conclusion Warming-needle is an effective therapy for primary dysmenorrhea with cold eougulation and blood stasis

Objective To investigate the refining and concentration of the Banlangen aqueous extractives using membrane separation technology. Methods The membrane separation process for refining and concentration Banlangen aqueous extractives was optimized by the quality of products and membrane operation parameters, and was tested through medium-scale trials. Results The selected G2+GM+RO2 membrane process was suitable. The inherent quality of the products obtained by membrane separation process was better than that obtained by traditional process. Conclusion G2+GM+RO2 membrane process is suitable to refine and concentrate Banlangen aqueous extractives, and it has technical conditions for industrial scale trials.

Objective To detect the expression of serum soluble E?cadherin(sEC)before and after operation in breast cancer patients,and to ex?plore its clinical significance. Methods The levels of sEC in 42 patients with breast cancer and 18 patients with benign breast diseases were tested by enzyme?linked immunosorbent assay(ELISA)before and after operation as well as in 30 healthy controls. Its correlation with clinical and patho?logical characteristics was analyzed. Results The levels of sEC in breast cancer patients before operation were significantly higher than patients with benign breast diseases and controls,which declined significantly after operation. Moreover,the levels of sEC in those with high clinical grading and lymphatic metastasis were also higher than in the ones with low grading and negative lymphatic metastasis(P<0.05). Conclusion The levels of sEC in breast cancer patients before operation are specific and closely related with pathological grade and lymphatic metastasis,which may be a pre?dictive factor for prognosis of breast cancer.

The overexpression of P-glycoprotein(P-gp)and its coding gene mdrl is regarded as the classic mechanism of muhidrug resistance(MDR).Recent studies find that pregnane X receptor(PXR)can mediate the expression of P-gp.It is confirmed that PXR can also inhibit apoptosis of tumor cells.Therefore,preventing the activation of PXR specifically may be a new method to prevent MDR.