1.Effect of Radix Astragali on the Transmission Small Intestine in Health Subjects
Yongfang QIAO ; Fohu JIANG ; Baoqi KONG
Traditional Chinese Drug Research & Clinical Pharmacology 1993;0(03):-
Objective: To observe the effect of Radix Astragali on the transmission time of small intestine(TTSI).Methods: TTSI and the peak value of lactose absorption were determined by hydrogen breath test in 40 healthy subjects a week before and after administration of Radix Astragali. Results: TTSI was 116.48+ 24.57 min and 102.38+ 13.44 min respectively before and after administration (P 0.05).Conclusion: Oral administration of Radix Astragali for one week has no effect on the absorption of the lactose by small intestine,but can promote the movement of small intestine.
2.Numerical simulation of semi-circular section stent grafts
Jingjing KONG ; Hongbin ZHANG ; Aike QIAO
Chinese Journal of Tissue Engineering Research 2009;13(48):9459-9462
Geometrically virtual models of three-dimensional blood flow field and eight types of mesh stent (different porosity and different stent shape) were constructed using SoildWorks software, and simulation of stented models were performed respectively using finite element software ANSYS11.0 according to computation fluid dynamics method. The flow resistance of the semi-circular section stent model of blood flow from the semi-circular planar surface was greater than the reverse in a certain range of permeability. However, the result was the opposite when the porosity increased to a specific value. For both grid-stent model and sine-stent model, flow resistance of blood that flowed from the stent in both directions increased with the porosity reduction. Under the same porosity and the direction of blood flow from the same stent, the flow resistance of the grid stent model was 1.5 times greater than the sine-stent model. Results showed that there are different effects on the flow resistance in blood flow in different directions from the semi-circular section stent model.
3.Pharmacoeconomic Analysis of Treatment of Child Bronchopneumonia With Four Varieties of Cephalospo-rins
Xiaoyun QIAO ; Daiyan KONG ; Yaping SHAO
China Pharmacy 1991;0(01):-
OBJECTIVE:To carry out the cost-effectiveness analysis of treating child bronchopneumonia with cefazolin,cefatrizine,cefataxime and cefuroxime METHODS:Using retrospective study and cost-effectiveness analysis,the costs of tre_atment of children with bronchopneumonia were calculated and pharmacoeconomically evaluated RESULTS:The costs of cefazolin,cefatrizine,cefuroxime and cefataxime in treatment of child bronchopneumonia were RMB 512 84,725 42,796 28 and 734 16 yuan and the cost-effectiveness ratios were 93 68,475 83,517 30 and 412 28 respectively CONCLUSION:In comparison with cefatrizine,cefuroxime and cefataxime,cefazolin is the most inexpensive one However,in evaluating the therapeutic cost of a drug, we should consider its therapeutic efficacy and course
4.Relationship of MR diffusion tensor imaging for corpus callosum and cingulate bundles with cognitive impairment in SIVD patients
Shuhu ZHOU ; Baojun QIAO ; Qinxia KONG
Chinese Journal of Geriatric Heart Brain and Vessel Diseases 2017;19(5):517-520
Objective To study the relationship of MR diffusion tensor imaging for corpus callosum and cingulate bundles with cognitive impairment in subcortical ischemic vascular dementia (SIVD) patients.Methods Sixty elderly SIVD patients admitted to our hospital from July 2015 to June 2016 served as a SIVD group and 40 age-matched persons undergoing physical examination served as a control group in this study.The mean ADC and FA were calculated according to the MR diffusion tensor imaging parameters in region of interest.Relationship between fiber tract integraty and MMSE Scale score was compared between the two groups.Results The ADC was significantly higher while the FA was significantly lower for the genu of corpus callosum in SIVD group than in control group.However,no significant difference was found in ADC for the spenium of corpus callosum between the two groups (P>0.05).The ADC was significantly higher while the FA was significantly lower for bilateral cingulate bundles (P<0.05,P<0.01).The FA for the genu of corpus callosum and bilateral cingulate bundles was positively related with the MMSE Scale score (r=0.511,r=0.469,r=0.457,P<0.05).However,the FA for the splenium of corpus callosum was not related with the MMSE Scale score(r=0.364,P>0.05).Conclusion Changes of MR diffusion tensor imaging parameters in corpus callosum and cingulate bundles contribute to the early prevention and treatment of cognitive impairment in SIVD patients.
5.Bovine Herpesvirus 1 Protein bICP0 Represses the Transcription of bISG15 in Fetal Bovine Lung Cells
Chang LIU ; Xiaohong KONG ; Wentao QIAO ; Yunqi GENG
Virologica Sinica 2011;(6):403-408
The ubiquitin-like modifier bISG15 is an antiviral protein found in fetal bovine lung (FBL) cells.Bovine Herpesvirus 1(BHV-1),which is a viral pathogen of cattle,can infect FBL cells and induce cytopathic effects.Real-time PCR assays showed that BHV- 1 's infection could repress the basal or inducible transcription of bISG15 in FBL cells.It demonstrates that this repression effect depends on BHV-1 viral infection and new protein synthesis.Our previous work showed that bIRF-3 was the key factor in the stimulation of bISG 15 in FBL cells,so the effect of BHV-1 viral protein on bIRF-3 activating the promoter of bISG15 was confirmed.The luciferase assay showed the BHV-1 viral protein bICP0 inhibited the activation of bISG15 promoter stimulated by bIRF-3.Taken together,our work suggested that BHV-I had some molecular mechanism to resist the cellular bISG15'santiviral functions.
6.Determination of Shionone in Rat Plasma by HPLC and Its Pharmacokinetic study
Yaping TIAN ; Qiao WANG ; Wei YANG ; Dezhi KONG ; Lantong ZHANG
Chinese Herbal Medicines 2010;02(2):132-135
Objective To develop a sensitive, simple, and accurate method for the determination of shionone in rat plasma after ig administration of Asteris Radix petroleum ether extract (RAPE). Methods The separation was achieved by HPLC on a RP18 column (150 mm × 3.9 mm, 5μm) with a mobile phase composed of acetotitrile-0.05% phosphoric acid water (98: 2) at a flow rate of 1.0 mL/min. UV Detector was set at 200 nm and friedelin was chosen as an internal standard. Results The linear range of the standard curves was (0.3443-22.0) μg/mL with the correlation coefficient of 0.9968. The intra- and inter-day precisions were all below 10% and the relative error was -3.5%-1.1%.Conclusion The developed method can be successfully applied to the pharmacokinetic study. After ig administration of RAPE, T1/2(ka) is (33.09 ± 7.32) min and T1/2(ke) is (84.95 ± 22.34) min.
8.Construction of pGL3-Basic-SREBP-1c-promoter reporter gene vector and detection of its function
Xiaojun LIU ; Xingxing KONG ; Rui WANG ; Di SHAO ; Aijun QIAO ; Yongsheng CHANG ; Fude FANG
Basic & Clinical Medicine 2006;0(05):-
Objective To construct human SREBP-1c-promoter reporter gene vector and to detect its function.Methods Human blood genome DNA was extracted and pGL3-Basic-SREBP-1c-promoter reporter gene vector was constructed.Furthermore,the function of SREBP-1c-promoter was confirmed by dual-luciferase reporter assay.ResultspGL3-Basic-SREBP-1c-promoter reporter gene vector was successfully constructed and the promoter activity was obviously repressed by co-transfection FoxO1.Overexpression FoxO1 inhibited the SREBP-1c protein expression.Conclusion FoxO1 repressed the SREBP-1c protein expression through inhibition the SREBP-1c transcription.
9.Recurrent laryngeal nerve injury during total thyroidectomy
Jisheng HU ; Rui KONG ; Gang YANG ; Xu WANG ; Na QIAO ; Bei SUN ; Linfeng WU
Chinese Journal of General Surgery 2015;30(9):683-686
Objective To investigate the mechanism of recurrent laryngeal nerve injury during total thyroidectomy.Methods The clinical data of 36 patients suffering from recurrent laryngeal nerve injury in total thyroidectomy from 2003 to 2014 was analyzed retrospectively.Results 21 cases had temporary recurrent laryngeal nerve injury while 16 cases had permanent injury.The cases of injury included giant thyroid goitre,with variation of recurrent laryngeal nerve,undergoing secondary surgery,with tumor invasion and complicating thyroiditis.During the first surgery,the incidence of temporary recurrent laryngeal nerve injury was 0.43%,and the incidence of permanent damage was 0.27%.The temporary and permanent injury incidence of secondary surgery after initial subtotal thyroidectomy was 4.59% and 4.59%,respectively.For patients with grade 3 thyroid gland,that was 1.81% and 0.30%,respectively and 0.51%,0.72% respectively in malignant cases.In cases complicating thyroiditis temporary recurrent laryngeal nerve injury occurred in 1.01%.Conclusions There is increased risk of recurrent laryngeal nerve injury in giant goiter cases undergoing secondary operations.Hence it is suggested that initial surgical procedure be hemithyroidectomy or total thyroidectomy
10.Treatment of severe cellulitis of the mouth floor:A retrospective study of 41 cases
Bo QIAO ; Junrui ZHANG ; Bin LU ; Liang KONG ; Fang LIU ; Xinghua FENG
Journal of Practical Stomatology 2015;(5):682-685
Objective:To analyze clinical data of 41 patients with severe mouth floor cellulitis.Methods:Patient's gender,age, epidemiological characteristics,origin of inflammation,symptoms of the disease,primary diagnosis,concomitant diseases,complica-tions,clinical diagnosis,the use of antibiotics (type and course),operation and outcomes were reviewed.Results:The male-fe-male ratio of the patients was 2.4∶1 .Age distribution was 1 0 -93 years old(with the mean of 46.23).The cases from urban-rural area was 3∶1 ,1 4.6% of the patients'primary diagnosis was not accurate.Only 31 % of the patients went to doctors in the first 5 days from the beginning of the disease.82.9% of the mouth floor cellulites were odontogenic.35% of the cases were diagnosed to be in-fectious diffusion to parapharyngeal,pterygomandibular spaces or to be complicating with neck infection at first examination.Infec-tion of 7.5% of the cases were spread to mediastinum.All the patients were administered with antibiotics and completed the surgical drainage as early as possible,except 2 transfered to respiratory medicine for mediastinum serious mediastinal and pulmonary infection and 1 to urinary surgery for renal failure.Conclusion:Odontogenic infection is most common for mouth floor cellulitis.Accurate di-agnose,maintenance of airway and initiate suitable antibiotics are rery important for the treatment of severe cellulitis of mouth floor. Prompt surgical drainage and comprehensive treatments are also essential to prevent patients from severe complications.