Objective To study the signal pathway of apoptosis of renal proximal tubular cells (RPTC) caused by cisplatin for prevention of toxic effects of cisplatin on kidney. Methods pcDNA3.1/Hygromycin vector and pEGFP-C3 vector including Bcl-2(Bcl-acta,Bcl-cb5 and Bcl-nt) were co-transfected in RPTC.After treated with cisplatin, Bax was activated,cytochrom C release and apoptosis were analyzed with confocal microscope and immunofluoresence technique. Apoptotic cells stained with Hoechst33258 were also counted and statistically analyzed. Results The percent of cytochrom C release (35.74%) in Bcl-cb5 transfected group was higher than those in Bcl-nt group (18.7%) and Bcl-acta group(24.6%)(P

Objective To evaluate effects of two different flap designs (envelope flap and triangular flap) on complica?tions after the mandibular third molar surgery. Methods A randomized, self controlled clinical trial design was selected for 52 patients treated in the outpatient surgery of Stomatological Hospital Affiliated to Tianjin Medical University. Patients were treated with envelope flap design for lower third molar removal in one side and triangular flap on the other side. VAS scores were used to evaluate postoperative pain. The postoperative swelling was evaluated by patient`subjective index. The degree of the upper and lower incisor distance was used to evaluate trismus. Data of postoperative swelling, pain and trismus were re?corded 1, 2 and 7 days after surgery. Data of postoperative wound dehiscence, bleeding situation and alveolitis were also re?corded and compared between two groups. Results There were no significant differences in postoperative pain after 1, 2 and 7 days between two flap designs (P>0.05). After 1 and 2 days there was more severe facial swelling in triangular flap group than that of envelope flap group (Z=2.005, Z=2.017, P<0.05). There was more limited mouth opening after 1 and 2 days in triangular flap group than that of envelope flap group (t=2.578, t=2.410, P<0.05), but on the seventh day there was no significant difference in the severity of facial swelling and trismus between two groups (P>0.05). There were no signifi?cant differences in postoperative pain, alveolitis, bleeding and wound dehiscence between two groups (P > 0.05). Conclu?sion The envelope flap is more conductive to the early recovery in patients after surgery, but in the long term there is no ob?vious difference between the two flap designs.

Objective To explore the expression of the mRNA level of Fas (CD95) ligand/FasL and Fas-associated phosphatase-1/FAP-1 in acute myeloid leukemia. Methods The expression of FasL and FAP-1 were detected in 54 patients with AML and 10 normal subjects by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). β-actin used as internal reference. The changes of FasL was observed after induction chemotherapy in 16 AML patients. The expression of Fas was detected in 54 patients with AML by flow cytometry. Results The mRNA levels of FasL and in 54 patients were remarkably higher(P <0.05) than the normal controls. The rates of first complete remission(CR) were significantly higher in FasL(+) (77.78 %) than in FasL(-) cases(16.67 %) (P<0.01). The FasL level declined in 10 patients with good response to therapy (P<0.01), and high level in 6 nonresponding patients with poor response (P>0.05). The mRNA levels of FAP-1 in 54 patients was remarkly higher than that of the normal control. 8/29 cases in Fas-positive group were positive for FAP-1 mRNA expression. 19/25 cases in the Fas-refractory group didn't express FAP-1 mRNA. Conclusion The expression of FasL was high in AML. The rates of complete remission were high in FasL positive cases. The FasL level declined in patients with good response to therapy. The expression of FAP-1was partly expressed in AML. The expression of FAP-1 was less in Fas positive group.

Objective To study the effects of preoperative psychological state on calm time、recovery time and the level of pain about female tumer patients with general anesthesia.Methods We selected 105 female tumer patients whose ages were from 39 years to 66 years.They were divided into two groups according to SAS grade.We observed the calm time,recovery time and the VAS about the two groups.Results The calm time and reeovery time in disquieting group was significant longer than that of the normal group.There was a significant difference between group A and group B(P＜0.01).Otherwise the grade of VAS after operation was significant greater than that of the normal group.Conclusion The disquieting preoperative psychological state would effect the results of general anesthesia.The anesthetist should pay attention to the visiting patients before operation.

Abstract: Objective　To investigate the status of participation in highly active antiretroviral therapy (HAART) among individuals infected with human immunodeficiency virus (HIV)/patients with acquired immunodeficiency syndrome (AIDS) (hereinafter referred to as HIV/AIDS) in Hainan province, and to analyze the immunological outcomes and their influencing factors following treatment. Methods　In this study, HIV/AIDS patients treated in Hainan Province until 2022 from the antiretroviral treatment were selected from the antiviral treatment submodule of the China Disease Prevention and Control Information System. According to the inclusion and exclusion criteria, a total of 3 150 HIV/AIDS patients were enrolled. Logistic regression analysis was used to analyze factors affecting the immunological effect of antiretroviral therapy. Results　Of the 3 150 subjects, 2 906 achieved immunological success, accounting for 92.2%. The M（P25，P75） CD4+T lymphocyte count after treatment was 491 (351, 656) cells/μL. There were significant differences in the increase in CD4+ T lymphocyte counts after treatment compared to baseline across different CD4+T levels (P<0.001). Multifactorial logistic regression analysis showed that being divorced or widowed (OR=0.50, 95%CI: 0.31-0.79), age ≥60 years at the onset of treatment (OR=0.51, 95%CI: 0.29-0.90), the interval from diagnosis to treatment of 1 to <12 months (OR=0.55, 95%CI: 0.40-0.77), 12 to <60 months (OR=0.53, 95%CI: 0.34-0.80), and ≥60 months (OR=0.41, 95%CI: 0.23-0.75), as well as a CD4+ T cell count of 300 to <400 cells/μL (OR=0.36, 95%CI:0.22-0.58) and 400 to <500 cells/μL (OR=0.30, 95%CI:0.18-0.49) were negative correlation with immunological success (P<0.05). On the other hand, homosexual transmission (OR=3.46, 95%CI:2.03-5.90), heterosexual transmission (OR=1.91, 95%CI: 1.14-3.19), other/unknown transmission (OR=3.13, 95%CI: 1.50-6.53), treatment years count 2 to <4 years (OR=1.71, 95%CI: 1.13-2.58), 4 to <6 years (OR=2.86, 95%CI: 1.81-4.51), and ≥6 years (OR=2.21, 95%CI: 1.47-3.32) were positive correlation with immunological success (P<0.05). Conclusions　The highly active antiretroviral therapy has achieved good immunological effects among patients with HIV/AIDS in Hainan Province. It is essential to expand HIV monitoring and detection, to diagnose and treat at an early stage, and to continue strengthening medication compliance education to further improve the immunological effects of treatment.

Objective: To study the influence of screening dementia with mini-mental state examination (MMSE) combining with delay memory test . Methods: MMSE and delay memory test are used in screening 301 residents. The diagnosis of dementia is according to the DSM-Ⅲ-R criterion. The validity and reliability were studied when the MMSE and delay memory test were used to screen dementia alone or combined. Results: When MMSE was used to screen dementia alone, the specificity, sensitivity, false positive proportion and false negative proportion were 85.40%, 52.43%, 47.56% and 14.60% respectively. When delay memory test was used to screen dementia alone, the specificity, sensitivity, false positive proportion and false negative proportion were 74.45%, 92.07%, 7.93% and 25.55% respectively. If parallel connection of MMSE and delay memory test were used to screen dementia, the specificity, sensitivity, false positive proportion and false negative proportion were 95.62%, 49.39%, 50.61% and 4.38% respectively. If MMSE was in series with delay memory test, the specificity, sensitivity, false positive proportion and false negative proportion of screening dementia were 64.23%, 95.73%, 4.27% and 35.77% respectively. Conclusion: The parallel connection of MMSE and delay memory test can elevate sensitivity of screening dementia and decrease false negative proportion, so it is important in epidemiologic investigation. MMSE in series with delay memory test will raise specificity of screening dementia and decrease false positive proportion, so it is useful for diagnosing early dementia.

ObjectiveElectric stimulation of the central nervous system has been served as a treatment method for variety of neurological,psychiatric and sensory disorders.Despite considerable success in some applications,current stimulation techniques offer little control over which neuronal targets are activated by stimulation.This study aimed to present a new shape self-adaptive helix electrode for selective activation of optic nerve.MethodsThe geometric model of optic nerve and new helix electrode was elaborated with COMSOL Multiphysics.The new helix electrodes consist of silicone helix frame,which acted as support and insulation,and platinum contacts embedded within the frame.The activating function (AF) was introduced to characterize the stimulation effects,and the selectivity of activating optic nerve fascicle with new helix electrode was simulated by COMSOL Multiphysics,taking into account the variations of electrode contact locations.ResultsAssuming normalized AF threshold was 0.1 V/m2,the ratio difference of AF over threshold between new helix electrode and traditional electrode was only 1.2410％.With contacts in two ends of helix electrode closer to the middle contact,the small nerve fascicle was first activated and then the large one.ConclusionThe results show that the new helix electrodes have the same stimulation effects as that of traditional cuff electrodes.The new helix electrodes can selectively activate optic nerve fascicle with variations of electrode contact locations.

Objective To evaluate effects of different types of gingival flap suture on the quality of life in patients after removal of mandibular third molar. Methods A randomized, controlled clinical trial design was adopted. Sixty-two patients in oral surgery department, Stomatology Hospital of Tianjin Medical University were selected. Patients were required removal of bilateral symmetry impacted mandibular third molars, and enclosed suture (primary suture) was used in one side, open drainage suture (secondary suture) was used in the other side. The postoperative symptom severity scale (PoSSe scale) was used to evaluate the quality of life in patients after removal of mandibular third molar. The evaluation was completed 7 days after surgery, and the scale was compared between two groups. Results A total of 57 cases of clinical data were enrolled in the final analysis. The operative time for primary suture group was (17.7±4.6) min, and the operative time for secondary su?ture group was (16.3 ± 5.1) min, the difference was not statistically significant (t=1.539, P>0.05). The PoSSe score was sig?nificantly higher in primary suture group (26.348 ± 12.414) than that of secondary suture group (21.633 ± 11.382, P<0.05). Conclusion Secondary suture reduces the influence of teeth removal on quality of life in patients through relieving pain and swelling, and which is suitable for clinical application.

Objective To compare the clinical efficacy of multi-target lateral puncture combined with intracranial pressure monitoring in treatment of basal ganglia hypertensive cerebral hemorrhage. Methods Sixty-six patients of basal ganglia hypertensive cerebral hemorrhage, with bleeding volume over 40 ml were divided into experimental group (36 cases) and control group (30 cases) by random digits table method. Patients in experimental group underwent multi-target puncture combined with routine intracerebroventricular treatment of intracranial pressure monitoring,and patients in control group underwent frontotemporal craniotomy and small hematoma decompressive craniotomy. The operation time, length of stay, hematoma evacuation rate, catheter drainage time, total amount of mannitol, Glasgow Coma Scale (GCS) scores 3 days after treatment, complication rate and 3-month Glasgow Outcome Scale (GOS) scores were recorded and compared between two groups. Results The operation time, length of stay, hematoma evacuation rate 1 day after treatment, and total amount of mannitol in experimental group were significantly lower than those in control group: (67.5±8.0) min vs. (109.3±9.6) min, (18.6±4.2) min vs. (23.3±5.9) min, (59.7±9.2)% vs. (80.4±11.6)%, (668.6±83.5) g vs. (1 430.4±107.1) g, P<0.01. The hematoma evacuation rate 3 days after treatment, catheter drainage time and GCS scores 3 days after treatment between two groups had no significant differences (P>0.05). The GOS scores in experimental group: 5 points (9 cases), 4 points (10 cases), 3 points (8 cases), 2 points(5 cases), and 1 point(4 cases). The GOS scores in control group: 5 points (4 cases), 4 points (4 cases), 3 points (7 cases), 2 points (9 cases), and 1 point (6 cases). Long curative effect in experimental group was better than that in control group (Z =2.318, P =0.020). The incidence of intracranial air in experimental group was significantly higher than that in control group: 27.8%(10/36) vs. 3.3%(1/30), P<0.05. Other complications had no significant differences between two groups (P>0.05). Conclusions Multi-target lateral puncture combined with intracranial pressure monitoring in treatment of basal ganglia hypertensive cerebral hemorrhage has more advantages, including less trauma, wide surgical indications, short operation time and hospital stay, less postoperative mannitol, and decreased mortality rate. For older, patients with organ dysfunction, and patients who can not tolerate craniotomy, it is an effective treatment, and worthy of promotion.

Female ; Humans ; Menstruation ; physiology ; Menstruation Disturbances ; epidemiology ; etiology ; Occupational Health ; Stress, Psychological ; complications ; epidemiology ; physiopathology