To evaluate bioequivalence and safety of two kinds of metformin hydrochloride sustained-release tablets (test preparation vs reference preparation) under the condition of fed and single administration.A single center, randomized, open, single-dose, two-period, two-sequence, and double-crossover design was used.32 healthy subjects took 0.5 g of test preparation or reference preparation under fed and single-dose administration.4 mL of venous blood was collected from before administration (0 h) to 1, 3, 4, 4.5, 5, 5.5, 6, 7, 8, 9, 10, 12, 15, 24, 36 and 48 h after administration.The concentration of metformin in plasma samples was detected, and then the pharmacokinetic parameters were calculated by WinNonlin 7.0 software.When the 90% confidence intervals of cmax, AUC0-t and AUC0-∞ geometric mean ratio of test preparation and reference preparation were within 80.00%-125.00% equivalent intervals respectively, the bioequivalence of the two preparations was proved.One subject fell off due to adverse events.The main pharmacokinetic parameters of test preparation and reference preparation as follows: cmax were (0.68 ± 0.14) and (0.65 ± 0.11) mg/L, AUC0-t were (7.33 ± 1.65) and (7.00 ± 1.89) h·mg/L, AUC0-∞ were (7.39 ± 1.67) and (7.06 ± 1.91) h·mg/L, respectively.The 90% confidence intervals of the geometric mean ratio of the two main pharmacokinetic parameters were 101.45%-109.14%, 100.08%-112.32% and 100.24%-112.28%, respectively, which fell within the bioequivalence interval of 80.00%-125.00%.There were no serious adverse events and unexpected adverse events during the trial.The results show that test preparation and reference preparation are bioequivalent under fed and single-dose administration, safe and well tolerated in healthy subjects.

ObjectiveTo investigate the anti-inflammatory effect of the component compatibility of Gentianae Macrophyllae Radix and Clematidis Radix et Rhizoma on the rat model of rheumatoid arthritis （RA） and the mechanism. MethodSeventy-two SPF-grade SD rats （male and female） aged 5 to 6 weeks were selected. Except the blank group， the rat model of collagen-induced arthritis （CIA） was replicated by the type Ⅱ collagen induction method. The 64 rats after successfully modeling were randomly divided into model group， methotrexate group （0.375 mg·kg^-1）， gentianoside with magnoflorine group （150.454 1 mg·kg^-1+5.061 8 mg·kg^-1）， gentianoside with clematichinenoside AR group （150.454 1 mg·kg^-1+16.433 1 mg·kg^-1）， sweroside with magnoflorine group （3.455 8 mg·kg^-1+5.061 8 mg·kg^-1）， sweroside with clematichinenoside AR group （3.455 8 mg·kg^-1+16.433 1 mg·kg^-1）， swertiamarin with magnoflorine group （9.303 2 mg·kg^-1+5.061 8 mg·kg^-1）， and swertiamarin with clematichinenoside AR group （9.303 2 mg·kg^-1+16.433 1 mg·kg^-1）， with 8 rats in each group. Each group was given the corresponding medicinal solution or normal saline by gavage for 15 d. During the experiment， the general status， of rats in each group were observed and recorded. Tumor necrosis factor-α （TNF-α）， interleukin-1β （IL-1β）， rheumatoid factor （RF）， C reactive protein （CRP）， prostaglandin E₂ （PGE₂）， and anti-cyclic peptide containing citrulline antibody （anti-CCP Ab） in the serum of rats were measured by enzyme-linked immunosorbent assay （ELISA）. The histopathological changes in rat ankle joints were observed by hematoxylin-eosin （HE） staining. Immunohistochemistry （IHC） and Western blot were used to detect the protein expression of nuclear factor-κB （NF-κB） and vascular endothelial growth factor （VEGF） in rat ankle joints. Real-time fluorescence quantitative polymerase chain reaction （Real-time PCR） was used to detect the mRNA expression of NF-κB and VEGF in rat ankle joints. ResultCompared with those in the blank group， rats in the model group were in poor general conditions with significant foot-plantar swelling， and the content of CRP， anti-CCP Ab， and IL-1β in the rat serum was significantly increased （P<0.01）. In the model group， the tissue structure of the ankle joint was severely damaged， and the protein and mRNA expression of NF-κB and VEGF in the rat ankle joints were significantly up-regulated （P<0.01）. As compared with the model group， the general status of rats in each administration group was significantly improved. The levels of serum TNF-α， IL-1β， RF， CRP， PGE₂， and anti-CCP Ab were reduced to different degrees in these administration groups， among which the effects of the gentianoside with clematichinenoside AR group on down-regulating serum TNF-α and IL-1β， the gentianoside with magnoflorine group on down-regulating serum RF and CRP， the sweroside with magnoflorine group on down-regulating serum PGE₂， and the swertiamarin with clematichinenoside AR group on lowering serum anti-CCP Ab were better than those of administration groups. The histopathological changes in the ankle joint were improved to different degrees. The protein and mRNA expression of NF-κB and VEGF in rat ankle joints in the administration groups was significantly down-regulated （P<0.05， P<0.01）， and the swertiamarin paired with clematichinenoside AR group had the most significant effect. ConclusionThe component compatibility of Gentianae Macrophyllae Radix and Clematidis Radix et Rhizoma exerts a good therapeutic effect on the rat model of RA， and the compatibility of components from the two medicines has a multi-channel， multi-target， and synergistic effect. The five component compatibility patterns， namely gentiobioside with magnoflorine， gentiobioside with clematichinenoside AR， sweroside with clematichinenoside AR， swertiamarin with magnoflorine， and swertiamarin with clematichinenoside AR， all have potential advantages. The mechanism may be related to the reduction of inflammatory factor secretion and the inhibition of abnormal protein and mRNA expression of NF-κB and VEGF.

Objective:To investigate the clinical efficacy of ear acupoint pressing beans combined with escitalopram tablets in the treatment of depression.Methods:A total of 100 patients with depression who received treatment in the Department of Psychiatrics, The Third People's Hospital of Yongkang from October 2021 to February 2023 were included in this randomized controlled study. These patients were randomly divided into a control group and an observation group, with 50 patients in each group, using a random number table method. The control group was treated with escitalopram tablets, while the observation group was treated with ear acupoint pressing beans combined with escitalopram tablets. The two groups were treated for 8 weeks. Hamilton Depression Scale (HAMD), traditional Chinese medicine syndrome scores, Pittsburgh Sleep Quality Index (PSQI), serotonin levels, clinical efficacy, and adverse reactions were recorded in each group.Results:After treatment, the HAMD scores in each group were significantly decreased compared with those before treatment (both P < 0.001). After treatment, the HAMD scores in the observation group were significantly lower than those in the control group [(15.89 ± 1.08) points vs. (19.24 ± 1.27) points, t = 14.21, P < 0.001]. After treatment, the traditional Chinese medicine syndrome scores in each group were significantly lower than those before treatment (both P < 0.001). After treatment, the traditional Chinese medicine syndrome scores in the observation group were significantly lower than those in the control group [(6.83 ± 1.24) points vs. (9.78 ± 1.80) points, t = 9.54, P < 0.001]. After treatment, the PSQI scores in each group were significantly decreased compared with those before treatment (both P < 0.001). After treatment, the PSQI scores in the observation group were significantly lower than those in the control group [(7.00 ± 1.34) points vs. (9.48 ± 1.82) points, t = 7.76, P < 0.001]. After treatment, the levels of serotonin in each group were significantly decreased compared with those before treatment (both P < 0.001). The levels of serotonin in the observation group were significantly higher than those in the control group [(40.65 ± 5.37) μg/L vs. (32.52 ± 4.20) μg/L, t = 8.43, P < 0.001]. The total effective rate in the observation group was significantly higher than that in the control group [94% (47/50) vs. 80% (40/50), χ2= 4.33, P = 0.037]. The incidence of adverse reactions in the observation group was significantly lower than that in the control group [8% (4/50) vs. 10% (5/50), χ2= 0.12, P = 0.727]. Conclusion:The combination of ear acupoint pressing beans and escitalopram tablets shows good clinical efficacy in the treatment of depression. This combined therapy can reduce symptoms, improve sleep quality, increase serotonin levels and also has fewer adverse reactions, making it a worthy candidate for clinical promotion.

Objective:To analyze the diagnosis of 3 cases of leukemia applying for the diagnosis of occupational radiogenic neoplasms.Methods:Retrospective analysis the occupational history, the disease history and the probability of causation (PC value) information of 3 radiological workers.Results:Two cases' PC value of 95% confidence limit of were >50%, and they were diagnosed as radiogenic neoplasms. One case was <50% and diagnosed as nonoccupational radiogenic neoplasms.Conclusion:The probability of causation analysis has important guiding significance for the diagnosis of occupational radiogenic neoplasms. Radiological workers should improve their awareness of self-protection and reduce the occurrence of occupational diseases.

Objective:To analyze the diagnosis of 3 cases of leukemia applying for the diagnosis of occupational radiogenic neoplasms.Methods:Retrospective analysis the occupational history, the disease history and the probability of causation (PC value) information of 3 radiological workers.Results:Two cases' PC value of 95% confidence limit of were >50%, and they were diagnosed as radiogenic neoplasms. One case was <50% and diagnosed as nonoccupational radiogenic neoplasms.Conclusion:The probability of causation analysis has important guiding significance for the diagnosis of occupational radiogenic neoplasms. Radiological workers should improve their awareness of self-protection and reduce the occurrence of occupational diseases.

Vitreous is composed of transparent gel-like tissue with complex molecular structure,accounting for 80％ of the volume of the eyeball.In addition to support the eye structure,vitreous has important functions such as regulating eye development,refraction and cellular barriers,participating in intraocular oxygen metabolism and the development of retinal diseases.Age,myopia and systemic diseases and other factors have an impact on vitreous function.Intra-vitreous injection and vitreous surgery may interfere with vitreous function,damage the intraocular metabolic balance.To better maintain intraocular metabolic balance and visual function,it is important to study vitreous metabolic function carefully and intervene it cautiously.

Objective: To investigate the individual irridiateddose levelandhealth statusofoccupational externalexposureamong radiationworkers inThreeA hospitals ofZhejiangprovince,andprovideevidence for occupationalhealthmanagement. Methods: 367 different typesof radiationworkerswereexperienced thedose monitoring and health examination from January toDecember, 2015, according to the requirements of"SpecificationsofIndividualMonitoring forOccupationalExternalExposure"and"Specifications forOccupational healthsurveillance forradiationworkers".The resultsofdosemonitoring,chromosomeaberration rate, lensopacity rate,hemogramand thyroid functionwerestatisticallyanalyzed. Results: Theannualeffectivedoseamong radiation workers fromZhejiangprovince in2015was0.13mSv,98.91%of them less than1mSv,whichunder the limit standardofstate(20mSv/a).Thechromosomeaberration(dicentric)detection rateswere7.41%and4.35%, from nuclearmedicinegroupand interventionalgroup respectively,whichhigher thandiagnostic radiologygroup, the differencewasstatisticallysignificant(χ²=13.686,8.092,P<0.01).Besides,1caseofsuspiciouschronic radiation dermatitiswas found in the interventiongroup.Radiation lengthhadsignificanteffecton lensopacity rate(P<0.01), lensopacity increasedwith the increasing lengthof the linear trend (χ²trend=16.363,P<0.01),and the incidence ofabnormalthyroid function theabnormalrateof lymphocyte ratiohad significantdifferenceamong theagegroups (P<0.05). Conclusion Although, theoccupationalexternalexposureamong radiationworkersinThreeAhospitals ofZhejiangprovince issafe, long-term lowdosesofionizing radiation stillhascertainhealtheffectson the fieldof nuclearmedicineand interventionalradiologystaff,suchaseye lens,cytogenetics,nailsandskin.

BACKGROUNDBone marrow hematopoietic function suppression is one of the most common side effects of chemotherapy. After chemotherapy, the bone marrow structure gets destroyed and the cells died, which might cause the hematopoietic function suppression. Heme oxygenase-1 (HO-1) is a key enzyme of antioxidative metabolism that associates with cell proliferation and resistance to apoptosis. The aim of this study was to restore or resist the bone marrow from the damage of chemotherapy by the HO-1 expression of mouse mesenchymal stem cells (mMSCs) homing to the mice which had the chemotherapy-induced bone marrow suppression.

METHODSOne hundred and sixty female Balb/c mice (6-8-weeks old) were randomly divided into four groups. Each group was performed in 40 mice. The control group was intraperitoneally injected for 5 days and tail intravenously injected on the 6th day with normal saline. The chemotherapy-induced bone marrow suppression was established by intraperitoneally injecting cyclophosphamide (CTX) into the mice which performed as the chemotherapy group. The mMSCs were tail intravenously injected into 40 chemotherapically damaged mice which served as the mMSCs group. The difference between the HO-1 group and the mMSCs group was the injected cells. The HO-1 group was tail intravenously injected into the mMSCs that highly expressed HO-1 which was stimulated by hemin. The expression of HO-1 was analyzed by Western blotting and RT-PCR. Cell proliferation was measured using the 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. Histopathologic examinations were performed 1 week after injection.

RESULTSCompared with the control group, the expression levels of HO-1 mRNA and protein were significantly higher in the HO-1 group (all P < 0.05), even obviously than the mMSCs group. CTX treatment induced apoptosis and inhibited proliferation. After injected, the white blood cell (WBC), red blood cell (RBC) and platelet (PLT) declined fast and down to the bottom at the 7th day. The bone marrow structure was destroyed incomplete. In vitro, the survival rate of cells in chemotherapy group was less than 50% after 24 hours. In contrast, mMSCs could do a favor to the cellular cleavage and proliferation. They slowed down the cell mortality and more than 50% cells survived after 24 hours. The effects of blocking apoptosis and bone marrow recovery could be more effective in the HO-1 group. In the HO-1 group, it had observed that the bone marrow structure became complete and the hemogram closed to normal at 7th day.

CONCLUSIONSHO-1 played an important role in promoting the recovery of CTX-induced hematopoietic damage. We suggest that HO-1 is able to restore the functions of chemotherapy-induced hematopoietic damage.

Animals ; Apoptosis ; drug effects ; Blood Platelets ; drug effects ; Blotting, Western ; Bone Marrow ; drug effects ; enzymology ; Cell Proliferation ; drug effects ; Cells, Cultured ; Cyclophosphamide ; toxicity ; Erythrocytes ; drug effects ; Female ; Heme Oxygenase-1 ; genetics ; metabolism ; Leukocytes ; drug effects ; Mesenchymal Stem Cell Transplantation ; Mesenchymal Stromal Cells ; enzymology ; physiology ; Mice ; Mice, Inbred BALB C ; Reverse Transcriptase Polymerase Chain Reaction

Purpose To study the refractive state of silicone oil tamponade eyes. Methods To calculate the theoretical refractive state of eyes with silicone oil based on clinical visual optics and to perform retinoscopy on 48 silicone oil filled eyes with pars plana vitrectomy (PPV) and 45 ones with PPV plus lensectomy with retinal reposition, and then study theoretical and experimental differences of diopter in silicone oil filled eyes. Results Postoperative diopter of the former increases (+6.26?1.20) D than preoperative diopter, while that of the latter is (+11.40?2.22) D. Conclusion Hyperopic changes are found in silicone oil tamponade eyes, and the experimental values are lower than the theoretical ones. This may be helpful in predicting the change of diopter of silicone oil tamponade eyes.