Objective To estimate the effect of diversified training model in training nurses in operation room. Methods A total of 36 nurses during 2014-2016 were chosen by using diversified training model. The effect and degree of doctors′satisfaction were evaluated. Results After diversified joint training, the average specialist theoretical scores of orthopedic surgery specialist nurses in 2014, 2015, 2016 were 78.444±6.243,84.072±4.754,90.565±2.597; the operational skill scores were 79.444± 4.623, 84.472±2.922,90.118±2.713, the differences were statistically significant (F=58.156, 82.619,P＜0.05). Preoperative operating specification rates were 72.2% (26/36), 86.1% (31/36), 100.0% (36/36);intraoperative operating specification rates were 75.0% (27/36), 83.3% (30/36), 100.0% (36/36); surgical instrument operating specification rates were: 66.7% (24/36), 83.3% (30/36), 97.2% (35/36); surgical fit:72.2% (26/36), 88.9% (32/36), 97.2% (36/36), the differences were statistically significant(χ2=9.755-11.624, P＜0.05). Conclusions The diversified training model is a valuable method in training nurses in operation room.