Objective To construct small hairpin RNA (shRNA) expression plasmid targeting rat opticin gene. Methods Four pairs of opticin oligonucleotides were synthesized and inserted into the plasmid vector, resulting into four plasmids: shRNA-1, shRNA-2, shRNA-3 and shRNA-4. Then the four constructed shRNA expression vectors and empty vector were transfected into rat ciliary non-pigment epithelium (NPE) cells by lipofectmaine 2000. Non-transfected NPE cells were set as control group. The expression of opticin mRNA and protein were measured by Reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot respectively. Results The opticin mRNA expression of the shRNA-1,shRNA-2, shRNA-3, shRNA-4 group were decreased compared with the control group (F = 10. 239, P = 0. 000);the inhibitory rate were 85.7% ,62. 87% ,54.87% and 48.77% respectively. The opticin protein expression of the shRNA-1,shRNA-2, shRNA-3, shRNA-4 group were also decreased compared with the control group (F=17.870, P= 0.000);the inhibitory rate were 78.7%, 34.6%, 31.1% and 16.8% respectively.Conclusions The shRNA-1 expression plasmid has most potent inhibitory effect on opticin expression in rat ciliary NPE cells.

Objective To explore the effect of weightlessness simulation on processing speed of mental rotation of males in 72 h head-down tilt,in order to further reveal human performance changes caused by weightlessness.Methods During 72 h weightlessness simulation (9 am day 1 to 9 pm day 4,head-down tilt started from 9 pm day 1),16 male subjects were processed speed of rotation data byhand picture mental rotation (test 8 times),using self cross-references design,and the variation of processing speed of mental rotation by duration was analyzed.Results The linear regression analysis showed,the reaction time of each time point for each subject as the independent variable,the angle of rotation as the dependent variable,with the extension of simulated weightlessness time.The intercept(constant term of the regression equation),indicating the processing speed of non-rotation,were gradually increased with HDT time,but didn(t) reached significantly difference (F(3,14) =0.551,P =0.650) ;the slope(Regression coefficient of the regression equation),indicating the processing speed of rotation.were gradually increased with HDT time,and reached the significantly difference (F(3,14) =4.338,P =0.009).The value of intercept and slope was like upside-down U in three days,and reached the highest value at day 2.Conclusion Weightlessness simulation have affection on the speed of mental rotation,especially on the rotation speed.

Objectives To explore the relationships between the current physical exercise behaviors and psychological factors of middle school students,so as to provide scientific evidence for improving health education and carrying out targeted health intervention measures.Methods The Multi-stage stratified sampling and cluster sampling method was adopted to choose 3600 students from 12 junior and senior middle schools in Hangzhou,Wuhan and Xi'an.The date was collected using the adolescent physical exercise behavior-psychological measurement scale(Chinese version).The structural equation model was built to explore the relationships between psychological factors(including the change of strategy,decision balance positive effect,decision balance negative effect and self-efficiency)and physical exercise.Results The change of strategy has biggest effect on physical exercise behaviors,with the standardized direct effect value of 0.344.The standardized direct effect value of decision balance negative effect,decision balance positive effect and self-efficiency were 0.071,0.093 and 0.169 respectively.The negative correlation among the four dimensions was significant statistically.Conclusion Psychological factors have direct effects on the change of the behaviors,and the change of the strategy has the largest impact on physical exercise behaviors of middle school students.The four psychological factors are closely correlated with one another,which is important in formulating middle school sports programs to promote the physical quality of middle school students.

Objective To compare the effects of health education on fat intake behavior and its psychology in students with different BMI,provide evidence for improving individual healthy education.Methods A experimental epidemiology was used,Twenty-nine secondary school classes were randomly selected by multi-stage cluster sampling from two cities in Zhejiang province.Overweight or obesity group included all students who were judged overweight or obesity,normal group included students who were judged normal and basic matched by age,grade,sex and stage of baseline.Intervention based on transtheoretical mode was conducted on two groups and intervention results were measured by the adolescent fat intake behavior of psychological measurement scales (Chinese version).Chi-square tests,T-tests,non-parameters tests and binary logistic regression were performed for data analyses.Results Stage of students with overweight or obesity and with normal BMI all moved forward after intervention,while the proportion of stage advance for students with overweight or obesity was further than students with normal BMI.Change strategy(2.60±0.79),pros(3.58±0.90) and self-efficacy(3.01±0.72) of students with overweight or obesity better than students with normal BMI in baseline((2.34±0.86),(3.08±0.91),(2.79±0.81)) while difference was not significant after intervention.Logistic regression analysis discovered that the promotion of change strategy moved stage forward for students with normal BMI(OR=2.168,95％ CI:1.560-3.014)and promotion of self-efficiency moved stage forward for students with overweight or obesity (OR=1.801,95％ CI:1.026-3.159).Conclusion The intensity and duration of psychological intervention is essential to be reinforced for students with normal BMI,while for students with overweight and obesity,psychological intervention should be promoted in combination with social support.

Background The deposition of advanced glycation end products (AGEs) in lens is the risk factor of diabetic complications.Researches revealed that AGEs has autofluorescence.Crystallin is a longevity protein.AGEs accumulation is probably associated with diabetic retinopathy (DR).Objective This study was to evaluate the association of AGEs autofluorescence intensity with diabetes and with DR.Methods A cross-sectional study was carried out under the approval of Ethic Committee of Shenyang He Eye Hospital and informed consent of each patient.One hundred eyes of 100 patients with age-related cataract aged 50-70 years were included in He Eye Hospital from September to December 2015.The patients were divided into non-diabetes group (40 patients) and diabetes group (60 patients),and then the patients in diabetes group were subdivided into non-DR (NDR) group,non-proliferating DR (NPDR) group and proliferating DR (PDR) group according to the DR grading criteria,20 patients for each.Glycosylated henoglobin A1c (HbA1c) and fasting plasma glucose (FPG) were detected for each subject,and the lens autofluorescence was assayed with lens fluorescence biomicroscope (Clearpath DS120).The association of lens autofluorescence intensity with serum HbA1c level or DR severity was analyzed.Results The age and diabetes course were matched among the non-diabetes group,NDR group,NPDR group and PDR group (F=2.587,2.899,both at P＞0.05),and the FBS and HbA1c level were evidently higher in the NDR group,NPDR group and PDR group than those in the non-diabetes group (all at P＜0.01).The autofluorescence intensity of lens was (0.159±0.032),(0.256±0.024),(0.319 ±0.013) and (0.394±0.035) cd in the non-diabetes group,NDR group,NPDR group and PDR group,respectively,showing a significant difference among the groups (F =90.265,P =0.000).The autofluorescence intensity of lens in the NDR group,NPDR group and PDR group was significantly increased in comparison with the non-diabetes group and the autofluorescence intensity of lens was gradually increased with the severity of DR (all at P＜0.01).A positive linear correlation was found between autofluorescence intensity of lens and serum HbA1 c level in diabetes patients (r =0.654,P ＜ 0.05).Conclusions The autofluorescence intensity of AGEs in lens appears to be associated with the severity of DR and HbA1 c.The autofluorescence intensity of AGEs in the lens of diabetes patient is probably one of the evaluation indexes of early stage of DR.

Objective:To explore effects of family aerobic exercise combined with respiratory training on pulmonary function, self-care ability and quality of life in chronic obstructive pulmonary disease (COPD) patients with frailty.Methods:A total of 86 COPD patients with frailty who were treated in the Outpatient Department in the First Affiliated Hospital of Zhengzhou University from September 2018 to March 2019 were selected by the convenient sampling method. They were randomly divided into the control group ( n=43) and the observation group ( n=43) . The control group was given routine health education while the observation group was given family aerobic exercise combined with respiratory training guidance. After 3 months of intervention, pulmonary function meters were used to compare lung function indexes between two groups. Barthel index and COPD Assessment Test (CAT) were used to compare activity of daily living (ADL) and quality of life of patients. Results:38 patients in the observation group and 40 patients in the control group completed the study. After 3 months of intervention, there was no statistically significant difference in lung function indexes between the observation group and the control group ( P> 0.05) . After 3 months of intervention, the Barthel index score of the observation group (71.28±10.02) was higher than that of the control group while CAT score (13.68±5.16) was lower than that of the control group. The differences between the two groups were statistically significant ( t=-2.102, 2.736; P< 0.05) . Conclusions:Family aerobic exercise combined with respiratory training can improve the lung function of COPD patients with frailty and improve their self-care ability and quality of life, which has certain clinical application values.

Objective To explore the key techniques used for surveillance and disposal of infectious diseases,food poisoning and hospital infection to improve the ability of surveillance and disposal on public health emergency.Methods Framework on surveillance and disposal of infectious diseases,food poisoning and hospital infection was set up,based on literature review and expert group discussion.Delphi method and technique for order preference by similarity to ideal solution comprehensive evaluation method were used for ordering preference by similarity,to screen key techniques set for surveillance and disposal of the above said events.Results Framework to be used for selecting key techniques was designed,based on the classification of emergency events,processing cycle of emergency events and level of techniques.Twenty six public health experts were selected for a 2-round consultation,with their authority as 0.796.Ten key techniques with important significance for surveillance and disposal of infectious diseases,food poisoning and hospital infection were selected from each event.Among these key techniques,the early-warning system was recognized as the key technique,important for the surveillance and disposal of all three emergency events.Items as technology used for unknown pathogenic microorganism detection,personal protection,gene sequencing and tracing technology,microorganism molecular typing technology,nucleic acid detection technology etc.were the key techniques and need to develop for the surveillance and disposal of infectious diseases and iatrogenic infection.Data regarding key technologies on security and privacy,early warning and forecasting,field rapid detection were sorted out that all in need to improve the surveillance programs on disposal of infectious diseases and food poisoning.Data exchange appeared another key technique on infectious diseases,with toxin detection and other 5 techniques the key techniques for food poisoning.Data collection,standardization and other 3 techniques appeared as the key techniques on iatrogenic infection.Conclusions Key techniques were selected in this study to meet the requirements of surveillance,disposal of infectious disease,food poisoning and iatrogenic infection.Authority of the experts got improved in the two-round consultation,ensuring the reliability of the results of screening and providing a scientific basis for the further research and priority development of the key techniques.

Medical record information is the core data source for diagnosis related groups(DRG)payment, and high-quality of medical record information is the foundation for DRG payment. The development of medical record management towards medical record information management, pushes up the demand for high-quality medical record information management professionals in hospitals. The conventional practice of professional training had resulted in such setbacks as professionals mismatch, long pre-employment training period, and large demand for continuing education. Based on the triple helix model theory, the study established a production-education integration training mode of university-government-hospital for medical record information management professionals. Capacity development was set as the goal and job demand as the guidance, for effectively integrating the educational resources, and promoting the model of " posts linked to training, employment following graduation" production-education integration training. The aim is to expand the quantity of talent team and improve the quality of talent training, thus effectively easing the shortage of medical record information management professionals.

Objective? To investigate the effect of stage cognitive behavioral intervention on anxiety and quality of life in patients with esophageal cancer undergoing chemotherapy. Methods? By convenience sampling, totally 78 patients with esophageal cancer combined with anxiety who received chemotherapy in the First Affiliated Hospital of Zhengzhou University from June 2017 to June 2018 were selected as the study subjects. Among them, 40 patients admitted from June to December 2017 were taken as the control group and the other 38 patients from January to June 2018 were in the observation group. The control group was given conventional nursing, and the observation group was given cognitive behavior intervention at admission and within 2 weeks after discharge. The two groups were compared in terms of the anxiety score and quality of life before and after intervention. Results? A total of 36 cases in the control group and 32 cases in the observation group completed the study. Before intervention, there was no statistical difference between the two groups in the anxiety score (P＞0.05). After intervention, the anxiety scores in both groups decreased compared with those before intervention with statistical differences (P＜0.01); and the anxiety score of the observation group after intervention was (40.28±5.46), lower than (55.97±3.28)of the control group with statistical significance (P＜0.01). Before intervention, there was no significant difference in the total scores of quality of life and scores in each fields between the two groups (P＞0.05). After intervention, the score of social/family status field in the observation group was (18.68±6.27) and the total score was (69.85±6.96), both higher than those in the control group (P＜ 0.01), while the scores of physiological, emotional and functional status in the observation group were higher than those in the control group, but there was no statistically significant difference between the two groups (P＞0.05). Conclusions? Stage cognitive behavioral intervention can improve anxiety and quality of life in patients with esophageal cancer undergoing chemotherapy, which is worthy of clinical application.

Objective? To explore the influence of group education based on transactional analysis model on self-management ability and quality of life of patients with chronic obstructive pulmonary disease (COPD). Methods? By convenience sampling, COPD patients admitted in the First Affiliated Hospital of Zhengzhou University from January of 2017 to May of 2018 were selected as research objects and among them, 42 patients admitted from January to August of 2017 were taken as the control group and were given routine health education; 47 patients admitted from September of 2017 to May of 2018 were taken as the observation group and were given group health education underpinned by the transactional analysis model. The COPD patients were compared before and after intervention in terms of the score in Self-management Scale and COPD Assessment Test (CAT). Results? Before intervention, there was no statistical difference between the COPD patients in the two groups in terms of the scores in Self-management Scale and CAT (P＜ 0.05). After intervention, the scores of each dimension of COPD Self-management Scale in the observation group were higher than those in the control group, and the scores of CAT in the observation group were lower than those in the control group, with statistical significance (P＜0.05). Conclusions? Transactional analysis model is conducive to improving the COPD patients' self-management ability and their quality of life.