Objective To investigate the clinical effect of mifepristone in treatment of perimenopausal dysfunctional uterine bleeding.Methods 80 cases of patients with perimenopausal dysfunctional uterine bleeding were selected and randomly divided into control group and research group,with 40 cases in each group.The control group was in the diagnosis of curettage after 10 days of oral provera 10mg every night before going to bed,stopped drug application in 14 days,15 days to retreat hemorrhages oral provera 10mg/day,stayed for 10 days,repeated drug use 5 months.The research group was treated with oral mifepristone every night before going to bed since cleaning up the uterus,12.5mg,for 6 months.Outpatient follow -up for 6 months,the recent curative effect,long -term curative effect,hormone level changes,anemia and adverse drug reactions were observed.Results The total effective rate of the research group was 95.0%,which of the control group was 82.5%,there was statistically significant difference(χ2 =7.82, P =0.005);The recurrence rate of the research group was 5.0%,which of the control group was 15.0%,there was statistically significant difference(χ2 =5.56,P =0.018).The FSH,LH,E2 and P of the two groups were decreased, but which of the research group were lower significantly than those of control group,the differences were statistically significant (all P <0.05).The decreased degree of endometrium in the research group was larger than that of the control group,the difference was statistically significant(P =0.0001).Conclusion Treatment of mifepristone perimenopausal dysfunctional uterine bleeding is significant,safe and convenient,which is worthy of clinical application.

Objective Exploring different strengthening immune strategy on long-term immune memory effects for tuberculosis vaccine lays the theoretical foundation.Methods The fusion protein AMM,adjuvant DDA and BCG-PSN mixed built AMM subunit vaccine.Choose only 50 C57BL/6 mice (SPF),according to the table of random number method was divided into 5 groups and each group 10 only,the experiment 1 group injected mice to phosphate buffer solution (PBS) as control; Experiment 2 mice only injected BCG vaccine (BCG) from early; Experiment 3 mice BCG initial free,in 10 weeks with AMM subunit vaccine strengthen immunity; The four mice from early after BCG,respectively on 8 weeks,10 weeks with AMM subunit vaccine strengthen immune mice one time.Experimental 5 groups of mice after BCG initial free,respectively on 6 weeks,8 weeks,10 weeks with AMM subunit vaccine strengthen immune mice a.28 weeks to remove spleen lymphocytes in mice,add 2.5μg/ml Ag85B stimulus 72 h after collecting spleen cell culture supernatant,with ELISA test Ag85B specificity splenic lymphocyte caused IFN ppar-gamma level.Results Spleen cells by antigen Ag85B stimulation,BCG initial free,AMM subunit vaccine strengthen immune once,twice,three times group and PBS compared all had difference (P ＜0.01 orP ＜0.05) ;BCG initial free-AMM subunit vaccine strengthen immune once,twice,three times group and BCG compared all had difference (P ＜0.01 or P ＜0.05) ; BCG initial free-AMM subunit vaccine strengthen immune once and twice or three times group compared with difference (P ＜0.01 orP ＜0.05) ; Initial free-AMM subunit vaccine strengthen immune two group and three times than group difference was statistically significant (P ＞0.05).Conclusion Mice strong immune strengthened the number is not the more the better,the appropriate number of strengthen the immune to induce the most lasting immunity memory,subunit vaccine strengthen immunization strategy may affect the immune memory is one of the important factors.

Objective The purpose of study was to investigate the differences in the value of urine acute kidney injury ( AKI) biomarkers in the diagnosis and prognosis of AKI in patients with or without acute respiratory distress syndrome ( ARDS ) . Methods We collected the clinical data about 304 ICU patients, in-cluding 105 ARDS (49 in the lungs and 48 outside the lungs) and 199 non-ARDS cases.Using ELISA, we determined the levels of uN-GAL, uL-FABP, uKIM-1, and uIL-18 in the first 48 hours, compared the clinical data and AKI biomarkers between different groups of patients.We analyzed the differences in the diagnostic value of the AKI biomarkers using the ROC curve and their value in predicting hospital mortality by logistic regression analysis. Results Compared with the patients without AKI, the AKI cases exhibited a signif-icantly increased level of uKIM-1 (1.02 [0.57, 3.01] vs 4.68 [54.74, 270.54], P=0.000) in the ARDS group and that of uL-FABP in the non-ARDS group (102.69 [37.98, 348.09] vs 53.52 [10.86, 141.39], P=0.009).In the ARDS group, the area under the ROC curve (AUC) for the combined efficiency of the four AKI biomarkers was 0.81 (95% CI 0.70-0.92), markedly higher than that of uNGAL (0.57 [95%CI 0.43-0.70]), uL-FABP (0.55 [95%CI 0.39-0.71]), and uIL-18 (0.56 [95%CI 0.40-0.72]) alone (P<0.05), so was the AUC for the combined efficiency of the four biomarkers than that of each biomarker alone in the patients with ARDS in or outside the lungs (P<0.05).The OR value of uKIM-1 for predicting hospital mortality was 1.529 (95%CI 1.148-2.036) in the ARDS group, 1.593 (95%CI 1.070-2.369) in the patients with ARDS in the lungs, and 1.512 (95%CI 1.005-2.274) in those with ARDS outside the lungs. Conclusion There were differences of diagnostic and predictive value of Urine AKI biomarkers have different values in the diagnosis and prognosis of AKI in ARDS and non-ARDS patients and in those with ARDS in or outside the lungs.

In order to explore the chemical constituents of Sarcandra glabra (Thunb.) Nakai, 10 compounds were isolated and identified from the water extraction of fresh aerial parts of Sarcandra glabra by silica gel column chromatography, polyamide column chromatography, gel column chromatography, and preparative high-performance liquid chromatography, including 2 elemane-type sesquiterpenoid glycosides, 6 violet ketone sesquiterpenoid glycosides, and 2 phenolic acid compounds. Based on their physicochemical properties and NMR data, the above compounds have been identified as sarcaglaboside C (1), sarcaglaboside D (2), byzantionoside B (3), lauroside E (4), (4Z)-4-[(3S)-3-(β-D-glucopyranosyloxy)butylidene]-3,5,5-trimethyl-2-cyclohexen-1-one (5), dihydrovomifoliol-O-β-D-glucopyranoside (6), (+)-abscisyl-β-D-glucopyranoside (7), 9ξ-O-β-D-glucopyranosyloxy-5-megastigmen-4-one (8), rosmarinic acid methyl ester (9) and methyl isorinate (10). Among them, compounds 3 - 5 and 8 - 9 were firstly isolated from S. glabra. The discovery of these compounds further enriches the structural types of compounds in S. glabra plants and provides an important material basis for subsequent pharmacological activity research.

Objective:To compare the difference of HIV infection risk among student Men who have sex with men (sMSM) attending secondary vocational colleges or high school, sMSM attending universities or above, and non-student men who have sex with men (MSM).Methods:A cross-sectional study was conducted between July and October, 2019. Inclusion criteria of participants include: 15-24 years old, male at birth, had homosexual oral or anal intercourse before survey, self-reported HIV negative or unclear. Demographic information, HIV testing history and social media usage were collected by a self-reporting questionnaire, the HIV risk assessment tool was used for HIV infection risk assessment. We used Logistic regression to analyze the difference of HIV infection risk among student sMSM attending secondary vocational colleges or high school, sMSM attending universities or above, and non-student MSM.Results:Of the 1 707 participants, 45% (771 projects) reported homosexual debut before the age of 19, the proportion of HIV self-testing was 49% (833 projects), and 34% (587 projects) received facility-based testing. Comparing with sMSM attending universities or above, non-student MSM and sMSM attending secondary vocational colleges or high school had higher probability of medium and high HIV infection risk [ OR(95% CI): 1.53(1.10-2.12), 1.80(1.06-3.07), respectively], while the probability of medium and high HIV infection risk between sMSM attending secondary vocational colleges and non-student MSM was not statistically different. Conclusion:Non-student MSM and sMSM attending secondary vocational college or high school have higher risk of HIV infection, health staff should allocate more education resources to Non-student MSM and sMSM attending secondary vocational college or high school, in order to alleviate HIV threat to them.

Objective:To compare the difference of HIV infection risk among student Men who have sex with men (sMSM) attending secondary vocational colleges or high school, sMSM attending universities or above, and non-student men who have sex with men (MSM).Methods:A cross-sectional study was conducted between July and October, 2019. Inclusion criteria of participants include: 15-24 years old, male at birth, had homosexual oral or anal intercourse before survey, self-reported HIV negative or unclear. Demographic information, HIV testing history and social media usage were collected by a self-reporting questionnaire, the HIV risk assessment tool was used for HIV infection risk assessment. We used Logistic regression to analyze the difference of HIV infection risk among student sMSM attending secondary vocational colleges or high school, sMSM attending universities or above, and non-student MSM.Results:Of the 1 707 participants, 45% (771 projects) reported homosexual debut before the age of 19, the proportion of HIV self-testing was 49% (833 projects), and 34% (587 projects) received facility-based testing. Comparing with sMSM attending universities or above, non-student MSM and sMSM attending secondary vocational colleges or high school had higher probability of medium and high HIV infection risk [ OR(95% CI): 1.53(1.10-2.12), 1.80(1.06-3.07), respectively], while the probability of medium and high HIV infection risk between sMSM attending secondary vocational colleges and non-student MSM was not statistically different. Conclusion:Non-student MSM and sMSM attending secondary vocational college or high school have higher risk of HIV infection, health staff should allocate more education resources to Non-student MSM and sMSM attending secondary vocational college or high school, in order to alleviate HIV threat to them.

OBJECTIVETo lay a foundation for the study on good variety selection of Isatis indigotica, comparison of plant traits and quality evaluation of Isatis germplasm resources from different production area was conducted.

METHODField cultivation and randomized block experiment were adopted to compare those plant traits of leaf, root, silique and seed of Isatis from different production area and the content of R,S-goitrin and polysaccharide in the root was determined.

RESULTDifferent cultivation forms of Isatis had significant difference from each other in leaf, root, silique and seed, content of R,S-goitrin and polysaccharide in the root were also different. R,S-goitrin content in Isatis of Chinese cabbage leaf type ( production area: Yunnan ) was comparative higher, 0.59% , while polysaccharide content in autotetraploid Isatis was comparative higher, 8.68%.

CONCLUSIONAccording to the plant traits, Isatis were classified into three types: Chinese cabbage leaf type, cabbage leaf type and mustard leaf type, of which R,S-goitrin content in Chinese cabbage leaf type (production area: Yunnan) was comparative higher, while polysaccharide content in autotetraploid Isatis was comparative higher.

China ; Drugs, Chinese Herbal ; analysis ; Isatis ; chemistry ; growth & development ; Phenotype ; Plant Leaves ; chemistry ; growth & development ; Plant Roots ; chemistry ; growth & development ; Seeds ; chemistry ; growth & development

Objective To explore the application effect of MEWS and status-background-assess-recommendation (SBAR) in patients with urological disease.Methods A total of 200 patients with urological disease and complete medical materials admitted in our hospital aged ≥16 years were included in the study and were divided into observation group and control group according to different evaluation schemes.In the control group, the traditional methods were used to evaluate the vital signs and consciousness, while in the observation group, MEWS and SBAR were used for evaluation.The early warning signals in the two groups were compared, and the risk factors were evaluated.Continuous grading was used to estimate efficacy of the clinical intervention measures.Results The occurrence rate of potential risk factors in the observation group was significantly lower than that in the control group (P<0.05).The nursing satisfaction in the observation group was significantly higher than that in the control group (P<0.05).The preferable healing rate in the observation group was significantly higher than that in the control group (P<0.05).Conclusion The combined application of MEWS and SBAR can accurately evaluate the condition in patients with urological disease, contribute to the formulation of therapeutic schemes, improve the prognosis and satisfaction, and reduce the risk of condition deterioration.

In order to explore the chemical constituents of Sarcandra glabra(Thunb.)Nakai,10 compounds were isolated and identified from the water extraction of fresh aerial parts of Sarcandra glabra by silica gel column chromatography,polyamide column chromatography,gel column chromatography,and preparative high-performance liquid chromatography,including 2 elemane-type sesquiterpenoid glycosides,6 violet ketone sesquiterpenoid glycosides,and 2 phenolic acid compounds.Based on their physicochemical properties and NMR data,the above compounds have been identified as sarcaglaboside C(1),sarcaglaboside D(2),byzantionoside B(3),lauroside E(4),(4Z)-4-[(3S)-3-(β-D-glucopyranosyloxy)butylidene]-3,5,5-trimethyl-2-cyclohexen-1-one(5),dihydrovomifoliol-O-β-D-glucopyranoside(6),(+)-abscisyl-β-D-glucopyranoside(7),9ξ-O-β-D-glucopyranosyloxy-5-megastigmen-4-one(8),rosmarinic acid methyl ester(9)and methyl isorinate(10).Among them,compounds 3-5 and 8-9 were firstly isolated from S.glabra.The discovery of these compounds further enriches the structural types of compounds in S.glabra plants and provides an important material basis for subsequent pharmacological activity research.

In order to explore the chemical constituents of Sarcandra glabra(Thunb.)Nakai,10 compounds were isolated and identified from the water extraction of fresh aerial parts of Sarcandra glabra by silica gel column chromatography,polyamide column chromatography,gel column chromatography,and preparative high-performance liquid chromatography,including 2 elemane-type sesquiterpenoid glycosides,6 violet ketone sesquiterpenoid glycosides,and 2 phenolic acid compounds.Based on their physicochemical properties and NMR data,the above compounds have been identified as sarcaglaboside C(1),sarcaglaboside D(2),byzantionoside B(3),lauroside E(4),(4Z)-4-[(3S)-3-(β-D-glucopyranosyloxy)butylidene]-3,5,5-trimethyl-2-cyclohexen-1-one(5),dihydrovomifoliol-O-β-D-glucopyranoside(6),(+)-abscisyl-β-D-glucopyranoside(7),9ξ-O-β-D-glucopyranosyloxy-5-megastigmen-4-one(8),rosmarinic acid methyl ester(9)and methyl isorinate(10).Among them,compounds 3-5 and 8-9 were firstly isolated from S.glabra.The discovery of these compounds further enriches the structural types of compounds in S.glabra plants and provides an important material basis for subsequent pharmacological activity research.