Objective To investigate the difference and significance of the clinical index for the aged suffered from type 2 diabetes mellitus in southern part of Guizhou. Methods A total of 240 old patients of type 2 diabetes mellitus were divided into 5 groups according to the complications (non-complications, 43; cerebrovascular disease, 20; macroangiopathy, 43; peripheral neuropathy, 67; diabetic retinopathy, 67) and 5 groups according to the different nationalities (Han 90, Miao 60, Buyi 70, Shui 12, Other 8). The chemical index including serum FBG, HbAlc, TC, TG, and HDL-c of all patients were detected. The results were treated with t-test and X2 test. Results The serum levels of FBG, HbAlc, TC, TG in the diabetic patients with complications were higher than that of non-complication patients (P

Objective To set up a new real-time quantitative PCR method for the detection of minimal residual disease in chronic myeloid leukemia patients, and to assess the bcr/abl fusion gene expression in chronic myeloid leukemia patients before and after treatment with imatinib mesylate by real-time quantitative PCR method. Methods The bcr/abl fusion gene expression in 30 patients with bcr/abl-positive chronic myeloid leukemia was analyzed by using real-time quantitative reverse transcription PCR (RQ-PCR) method. The patients treated with imatinib in a dose of 400mg/d for 1 year and 2 years were also examined (8 cases for each). In 19 new patients the same study was also conducted. Results The real time quantitative PCR method could detect 10 copies in the test. The average bcr/abl expression levels in new patients or patients who had been treated with imatinib for 1 year and 2 years were 68.18%?26.67%, 0.16%?0.15% and 0.04%?0.02%, respectively. The average logarithm reduction values after treatment were 2.82 in the first year and 3.36 in the second year. In 25% of patients (4/16) negative FISH results could not be obtained, but it was much lower than that of before imatinib-treatment. When FISH became negative, RQ-PCR showed positive results. Conclusions RQ-PCR is a more sensitive technique in the detection of bcr/abl fusion gene than the FISH. It is an important way to monitor the tumor cell during the treatment with imatinib mesylate in chronic myeloid leukemia patients.

Background The conventional study of antigen-presenting cells(APCs)in eye relies on in vitro histoimmunochemistry,but its outcome is influenced by many factors.The anterior chamber injection of fluoresceinmarked antibody was used as a new approach before,however,it is liable to lead to injury of cornea.The intravitreal injection of fluorescein-labeled antibody may be important for the in vivo study of the phenotype features of APCs in iris,which is significant for evaluating the function of APCs in immune homeostasis.Objective This study was to investigate the phenotype characters,distribution and morphology of different types of APCs in the normal murine iris.Methods Fifty-one SPF female BALB/c mice(from 6-to 8-week old)were randomized into 17 groups according to the injection of different antibodies.Alexa Fluor 594 or Alexa Fluor 488-tagged ovalbumin (OVA),CD11 c,major histocompatibility complex Ⅱ (MHC-Ⅱ),F4/80,B7-1 and B7-2 monoclonal antibodies or mixtures of two antibodies (2.0 μl)were intravitreally injected at 0.5 mm far from corneal limbus with microneedle under the biomicroscope.The iris tissues were isolated 24 hours after injection.The phenotype characters,precise distribution and morphology of different types of APCs were identified by epifluorescence microscope and laser confocal microscope.In vitro staining was also performed to validate the in vivo staining results.Results After in vivo staining via intravitreal injection,the cell positive for OVA as well as MHC-Ⅱ,F4/80,CD11 c,B7-1 and B7-2 were exhibited with the regular networkline appearance throughout the normal murine iris.Positive cells tagged with Alexa Fluor 594 or Alexa Fluor 488 presented the red or green fluorescence.Double-fluorescein staining showed that about 90％ of F4/80+ cells were OVA+,and MHC-Ⅱ was expressed in about 60％ of F4/80+ cells and CD11c+cells,and about 35％ of F4/80+ cells and CD1 1 c+ cells expressed B7-1 and B7-2 simultaneously,and over 70％ of OVA+ cells were positive to MHC-Ⅱ.These labeled cells were identified as two populations based on their shape.One type was dendritiform cell (DC) with a small cell body and many long dendrites,including OVA+,CD1 1 c+,F4/80+ cells and MHC-Ⅱ + cells ; and the other types were polymorphic population being round,pleomorphic or irregular shape with a large cell body and a few short dendrities,including B7-1 + and B7-2+ cells.Conclusions In vivo intravitreal injection of labeled antibodies can be adapted to visualize the labeled cells in the murine iris.APCs with distinct morphologies,phenotypes and distribution may contribute to the immunologically privileged feature and inflammation of the eye.

Objective To explore the effect of reconstruction on old rupture of finger flexor tendon. Methods There were 30 patients (54 fingers) with old rupture of finger flexor tendon. The silica gel stick was implanted at the first stage. The palmaris longus tendon wrapped in the bioabsorbable membrane replaced the silica gel stick and was implanted at the second stage. The effectiveness was assessed by total active movement (TAM). Results There were 30 fingers as excellent, 20 fingers as good among 54 fingers of 30 patients. The percent of excellent and good was 92.6%. Conclusion Modified reconstruction is effective to prevent from tendon adhesion.

ObjectiveTo explore the diagnosis and prognosis value of clinical pneumonic infection score (CPIS) in patients with stroke-associated pneumonia (SAP).MethodsOne hundred and fifty stroke patients were evaluated and analyzed by CPIS.SAP was regarded as gold standard,and sensitivity,specificity,diagnose accordance rate,positive predictive value and negative predictive value were calculated.Results Thirty-nine patients had SAP and 111 patients did not have SAP by CPIS,and the incidence of SAP was 22.0％ (33/150).CPIS diagnostic sensitivity was 84.8％ (28/33),specificity was 90.6％ ( 106/117 ),positive predictive value was 71.8％ (28/39),negative predictive value was 95.5％ (106/111),and diagnose accordance rate was 89.3％(134/150).The patients of SAP were divided into good prognosis (28 cases) and bad prognosis (5 cases),and the CPIS was significantly lower in patients of good prognosis 7 days after SAP than that in patients of bad prognosis[(4.21 ± 2.23) scores vs. (6.05 ±2.32) scores,P ＜0.05].ConclusionsNot only CPIS has higher diagnosis rate in SAP incidence,but also has good judgment to prognosis.It is worthy of clinical application.

Objective To observe the effect of rosuvastatin calcium on lipid,vascular endothelial growth factor (VEGF),nitric oxide (NO),tumor necrosis factor (TNF)-α and interleukin (IL)-1 in hyperlipidemia patients.Methods One hundred and twenty-seven hyperlipidemia patients were randomly divided into two groups.Patients in the study group included 72 patients which were given rosuvastatin calcium 10 mg and enteric-coated aspirin 100 mg,orally,once a day for 8 weeks.The control group included 55 patients which were only given enteric-coated aspirin 100 mg,orally,once a day for 8 weeks.The change of lipid,VEGF,NO,TNF-α and IL-1 was observed before and after treatment.Results Before treatment,the level of total cholesterol(TC),triglyceride (TG),low density lipoprotein-cholesterol (LDL-C),high density lipoprotein-cholesterol (HDL-C),VEGF,NO,TNF-α and IL-1 in two groups had no significant difference (P ＞ 0.05).After treatment,the level of TC,TG,LDL-C,TNF-α and IL-1 in study group were significantly lower than those in control group [(4.410 ± 0.688) mmol/L vs.(6.491 ± 0.744) mmol/L,(1.762 ± 0.834) mmol/L vs.(2.632 ± 0.792) mmol/L,(2.256 ± 0.347) mmol/L vs.(4.544 ± 0.493) mmol/L,(41.14 ± 5.41) ng/L vs.(71.34 ± 6.76) ng/L,(0.22 ± 0.18) μ g/L vs.(0.42 ± 0.23) μ g/L] (P ＜ 0.05).The level of HDL-C,VEGF and NO in study group were significantly higer than those in control group [(1.807 ± 0.730) mmol/L vs.(1.432 ± 0.514) mmol/L,(564.86 ± 120.02) ng/L vs.(451.23 ± 100.72) ng/L,(42.39 ± 6.71) μ mol/L vs.(33.65 ± 6.24) μ mol/L](P＜ 0.05).No adverse reaction occurred in two groups.Conclusions Rosuvastatin calcium can obviously decrease the level of lipid,elevate the expression of VEGF and NO,and reduce the expression of TNF-α and IL-1.Rosuvastatin calcium can improve vascular endothelial function obviously in hyperlipidemia patients.

Objective To investigate the characteristics and the risk factors of pulmonary function in patients with chronic obstructive pulmonary disease (COPD) for a 3 year follow-up.Methods Subjects diagnosed as COPD were followed up for 3 years in the Management Center of Chronic Respiratory Disease at XINQIAO Hospital from September 2009 to June 2012.This was a retrospective study.Parameters related to respiratory function mainly first second forced expiratory volume (FEV1),COPD assessment test (CAT),6 minutes walking distance (6MWD) and acute exacerbation were recorded during follow-up.Results Although the majority of patients were treated with drugs such as inhaled corticosteroid combined with longterm bronchial dilatation during the three years,FEV1 decreased progressively.The average annual decline of FEV1 was(31.80 ± 61.99) ml,translating into a mean annual decline of(3.74 ± 6.18) ％.However,there were significant differences in changes of FEV1.Approximately,FEV1 in 78.3％ (47/60) patients decreased,only 21.7％ (13/60) patients kept stable FEV1.There was a correlation between decrease of FEV1,FEV1 ％ predicted and the exacerbation (r =0.298,0.361,0.273;P ＜ 0.05).Logistic regression showed that the positive bronchodilator reversibility and the initial FEV1 were the independent factors associated with significant changes in FEV1 (respectively,OR =5.54,95％ CI 1.55-19.73;OR =8.28,95％ CI 1.42-48.32).Conclusion The changes of pulmonary function in patients with COPD are heterogeneous,although most patients are treated in a standard way.Nearly 80％ patients still represent deterioration of pulmonary function.Decline of FEV1 is closely related to the initial pulmonary function and bronchodilator reversibility.

Objective:To systematically evaluate the clinical efficacy and safety of autologous platelet-rich concentrate in facial fat transplantation.Methods:By combining subject headings with free words, the randomized controlled trials of platelet-rich concentrate combined with autologous fat grafting (AFG) on improving facial morphology were searched in domestic and foreign databases (PubMed, Embase, Cochrane Library, Web of Science, CNKI, Wanfang Database, VIP Database) published in Chinese and English from database establishment to April 2023. According to inclusion and exclusion criteria, NoteExpress was used to screen relevant literatures, quality evaluation and data extraction were carried out for the final included literatures, and statistical analysis of the data was carried out using Review Manager 5.4 and Stata 15.0. For the count data, the risk ratio ( RR) was used as the statistical effect index, and the Mantel-Haenszel(M-H) hierarchical analysis method was used to calculate the combined results. For the measurement data, mean difference ( MD) was used as the statistical effect indicator, and the combined results were calculated by the inverse-variance(I-V) method. The heterogeneity among the studies was comprehensively tested by Q test combined with I2 statistic. If there was high heterogeneity among the studies, the random effect model was used for statistical analysis. Otherwise, the fixed effect model was used for statistical. Results:A total of 1 238 literatures were retrieved, and 19 randomized controlled trials involving 1 168 patients were eventually included after screening. The papers were all published within the last 10 years, mainly by Chinese authors. In the experimental group, the intervention measures of four studies were platelet-rich fibrin combined with AFG, two studies were concentrated growth factor combined with AFG, and the other thirteen studies were platelet-rich plasma combined with AFG. The results showed that: compared with fat transplantation alone, the addition of platelet-rich concentrate could improve the long-term fat retention rate ( MD=18.20%, 95% CI 14.03%-22.37%, P<0.001) and increase the operative response rate ( RR=1.22, 95% CI 1.15-1.29, P<0.001), shorten the postoperative recovery time ( MD=-4.18 d, 95% CI -7.01 d to -1.34 d, P=0.004), and reduce the incidence of secondary surgery ( RR=0.24, 95% CI 0.15-0.40, P<0.001), furthermore, patient satisfaction was improved ( RR=1.25, 95% CI 1.17-1.33, P<0.001). In addition, the incidence of complications during treatment was reduced ( RR=0.35, 95% CI 0.24-0.51, P<0.001). Conclusion:The efficacy and safety of platelet-rich concentrate assisted fat transplantation on face are worthy of recognition, and more large-scale and multi-center studies are needed for further clinical promotion.

Objective:To systematically evaluate the clinical efficacy and safety of autologous platelet-rich concentrate in facial fat transplantation.Methods:By combining subject headings with free words, the randomized controlled trials of platelet-rich concentrate combined with autologous fat grafting (AFG) on improving facial morphology were searched in domestic and foreign databases (PubMed, Embase, Cochrane Library, Web of Science, CNKI, Wanfang Database, VIP Database) published in Chinese and English from database establishment to April 2023. According to inclusion and exclusion criteria, NoteExpress was used to screen relevant literatures, quality evaluation and data extraction were carried out for the final included literatures, and statistical analysis of the data was carried out using Review Manager 5.4 and Stata 15.0. For the count data, the risk ratio ( RR) was used as the statistical effect index, and the Mantel-Haenszel(M-H) hierarchical analysis method was used to calculate the combined results. For the measurement data, mean difference ( MD) was used as the statistical effect indicator, and the combined results were calculated by the inverse-variance(I-V) method. The heterogeneity among the studies was comprehensively tested by Q test combined with I2 statistic. If there was high heterogeneity among the studies, the random effect model was used for statistical analysis. Otherwise, the fixed effect model was used for statistical. Results:A total of 1 238 literatures were retrieved, and 19 randomized controlled trials involving 1 168 patients were eventually included after screening. The papers were all published within the last 10 years, mainly by Chinese authors. In the experimental group, the intervention measures of four studies were platelet-rich fibrin combined with AFG, two studies were concentrated growth factor combined with AFG, and the other thirteen studies were platelet-rich plasma combined with AFG. The results showed that: compared with fat transplantation alone, the addition of platelet-rich concentrate could improve the long-term fat retention rate ( MD=18.20%, 95% CI 14.03%-22.37%, P<0.001) and increase the operative response rate ( RR=1.22, 95% CI 1.15-1.29, P<0.001), shorten the postoperative recovery time ( MD=-4.18 d, 95% CI -7.01 d to -1.34 d, P=0.004), and reduce the incidence of secondary surgery ( RR=0.24, 95% CI 0.15-0.40, P<0.001), furthermore, patient satisfaction was improved ( RR=1.25, 95% CI 1.17-1.33, P<0.001). In addition, the incidence of complications during treatment was reduced ( RR=0.35, 95% CI 0.24-0.51, P<0.001). Conclusion:The efficacy and safety of platelet-rich concentrate assisted fat transplantation on face are worthy of recognition, and more large-scale and multi-center studies are needed for further clinical promotion.

Objective To investigate the effect of radial artery calcification (RAC) on survival of arteriovenous fistula (AVF) and the patients in end?stage renal disease. Methods Adult ESRD patients undergoing AVF surgery between January 2013 and January 2016 at the Eighth Affiliated Hospital of Sun Yat?sen University were enrolled in this study. The clinical and biochemical data were collected. Segment of radial artery were obtained from the operation of AVF. RAC at the site of anastomotic were observed by alizarin red S and hematoxylin and eosin staining. According to RAC, the patients were divided into calcification group and non?calcification group. Kaplan?Meier analysis was performed to analyze the survival rates of the two groups, and Cox proportional hazards regression——model was used to estimate the risk factors of AVF dysfunction and all?cause mortality in ESRD patients. Results Among 180 cases of ESRD patients, 38 cases (21.1％) were developed RAC at the site of anastomotic in different degrees. Compared with the non?calcification groups, the calcification groups had a longer dialysis vintage, a higher proportion of diabetes and higher level of HbAlc (all P﹤0.05). Binary logistic regression analysis showed that dialysis vintage>5 years and diabetics were two independent risk factors of RAC at the site of anastomotic. Kaplan?Meier survival analysis demonstrated that there were no statistical differences between two groups in AVF survival (χ2=0.009, P=0.926). Calcification group had higher all?cause mortality than non?calcification groups (χ2=9.809, P=0.002). Multivariate Cox regression analysis demonstrated that homocysteine was independent risk factor for AVF dysfunction (HR=1.027, 95％CI: 1.003-1.051, P=0.027). Age was independent risk factor for all?cause mortality (HR=1.078, 95％CI: 1.035-1.122, P=0.000). Conclusions Dialysis vintage>5 years and diabetes were two independent risk factors of RAC at the site of anastomotic in ESRD patients. RAC at the site of anastomotic had no effect on AVF survival, but increased all?cause mortality.