1. Infiltration of tumor associated macrophages in multiple myeloma and its clinical significance
Qianle GUI ; Yansheng WANG ; Shan HUANG ; Yang WAN ; Huiping WANG ; Zigang ZHU ; Manman LI ; Haiyan ZHU ; Qianshan TAO ; Yuanyuan SHEN ; Qing ZHANG ; Hui QIN
Chinese Journal of Hematology 2018;39(2):122-127
Objective:
To investigate the clinical significance of tumor associated macrophages (TAM) in multiple myeloma (MM) and the relationship with angiogenesis and immunosuppression.
Methods:
Seventy cases of MM patients diagnosed from August 2015 to June 2017 were enrolled in the study as experimental group, 20 cases of benign hematological diseases (13 with iron deficiency anemia and 7 with megaloblastic anemia) patients as control group. Immunohistochemical method was used to detect the expression of CD163, CD34 and VEGF in bone marrow samples, and flow cytometry was used to detect the proportion of regulatory T cell (Treg cells), ELISA was used to detect the level of IL-10, and the clinical features were analyzed.
Results:
①Among the 70 patients, there were 31 males and 39 females with a median age of 65 (50~78) years old. TAM infiltration density, microvascular density (MVD), VEGF expression level, Treg ratio and IL-10 level in bone marrow samples of 70 MM patients were significantly higher than those of benign hematological diseases (