Kvl.3 potassium channel plays a key role in T lymphocyte activation.In many diseases mediated by T lymphocytes, such as multiple sclerosis, rheumatoid arthritis, systemic lupus erythematosus, anti-glomerular basement membrane glomerulonephritis, asthma, Kv1.3 potassium channel expression is up-regulated,closely related to the mechanism of those disease.In the cardiovascular system diseases, Kv1.3 potassium channel protein is related to the differentiation of monocyte derived macrophage into foam cells, and vascular remodeling.In addition Kv1.3 potassium channel plays a role in apoptosis.This paper provide new ideas for the diagnosis and treatment of T lymphocytes mediated disease, cardiovascular disease and neoplasms.

Objective To evaluate the concentration of urinary cadmium and serum sex hormone in cadmium-exposed male workers and explore the affecting factors and related mechanism.Methods The individuals exposed to cadmium from three workshops 40 in each, in a cadmium rechargeable battery manufacture plant were selected as the exposure groups according to concentrations of cadmium oxide in the workplace in November 2008, namely assembly, charging and butt-weld workshop for low, moderate and high dosage group.Another forty male workers without cadmium exposure of the same plant were selected as the control group.Urinary cadmium, serum testosterone,FSH and LH concentrations were measured and urinary cadmium concentration abnormity rate was calculated for the following analysis.Results Compared with the control group, the urinary cadmium concentration and its abnormity rate of the low and high dosage groups were all significantly increased(P9 years groups were all significantly increased(P

Objective Allergic asthma is a chronic inflammatory disease of the airways.T lymphocytes play important roles in the pathogenesis of asthma.The voltage-gated Kvl.3 potassium channel may be a key factor in the activation of T lymphocytes.This research aims to detect the function of Kvl.3 channel in the neutrophlial asthma(NA) model and eosinophilic asthma(EA) model.Methods A total of 24 mice were randomly assigned into three groups:control,neutrophilic asthma model and eosinophilic asthma group.Neutrophilic asthma model was established with ovalbumin (OVA)and lipopolysaccharide(LPS);eosinophilic asthma was established with OVA and Al(OH)3;airway responsiveness of mice in each group was measured with a noninvasive pulmonary function instrument;lung inflammation changes were observed by pathological HE staining;IL-17A and IL-4 cytokines levels in bronchoalveolar lavage fluid were evaluated by ELISA;Kvl.3 channel protein level in lung was evaluated by western blot;the change of current density in CD4 +.T lymphocytes were tested by whole-cell patch clamp technique.Results Levels of IL-17A and IL-4 in bronchoalveolar lavage fluid increased in both NA and EA model (P ＜ 0.05).Compare with EA model,the IL-17A level was significantly higer in NA model,while the IL-4 level was significantly lower.In NA and EA model,kv1.3 protein expression in lung tissue was significantly higher than that in the control group(P ＜ 0.05),and kv1.3 protein expression in NA model was significantly higer than that in the EA model (P ＜ 0.05).Current intensity and current density of kv1.3 channel increased in both NA and EA model.While the current intensity and current density of kv1.3 channel were significantly higher in NA model than that in EA model.Conclusion Kv1.3 protein level,Kv1.3 channel current intensity and kv1.3 channel current density increased in both NA and EA model,especially in NA group,providing a new way for treatment of bronchial asthma.

Objective To investigate wether bronchial epithelial-mesenchymal transformation (EMT)take part in airway remodling in asthmatic mice,and the effect of inhaled glucocorticoid to it. Methods Thirty BALB /c mice were randomly divided into control group(n =10),asthma group(n =10) and budesonide group(n =10).The qualitity of serum OVA-sIgE was measured to vertify asthma modle. Bronchial airway thickness collagen deposition area was analyzed by HE and Masson staining to test the level of airway remodling.ELISA was used to test the quantity of transforming growth factor(TGF)-βin bron-choalveolar lavage fluid(BALF).Immunohistochemical assay was used to observe fibroblast specific protein-1 (FSP-1 )expressing area.Western blot and realtime PCR were performed to analyze the protein and mRNA expression of E-caderin,Vimentin and FSP-1 .Results The expression of OVA-sIgE,bronchial airway thick-ness,the collagen deposition area were significantly higher in asthma group than those in control group,while the above index in BUD group were allivated compared to those in asthma group(P <0.01 ,respectively). The expression of TGF-βin BALF increased and the expression of FSP-1 located in bronchial epithlium,the quantity of E-caderin significantly decreased,and the quantity of Vimentin and FSP-1 increased by Western blot and realtime PCR in asthma group,compared to those in control group(P <0.01 ).While the level of E-caderin increased(P <0.01 ),TGF-βand Vimentin reduced partly in BUD group(P <0.05 ),and there was no differece in the level of FSP-1 between BUD group and asthma group(P >0.05).Conclusion Lung tissue EMT take part in airway remodling of asthma,which is mainly focus on bronchial epithlium.The effect of traditional inhaled budesonide can not heal the bronchial EMT in asthma.

Objective To explore effect of grape seed proanthocyanidin extract (GSPE)on airway inflammation and hyperresponsiveness of ovalbumin-induced murine asthma model and the associated regulatory effect on Treg/Th17 imbalance.Methods A total of 40 mice were randomly assigned to four experimental groups:control,asthma model,low dose GSPE (40 mg/kg),and high dose GSPE (80 mg/kg).Acute asthma model was established with OVA;airway responsiveness of mice in each group was measured with a noninvasive pulmonary function instrument;lung inflammation changes were observed by pathological HE staining;Treg/Th17 cytokines levels in bronchoalveolar lavage fluid were evaluated by ELISA;the expressions of forkhead/winged helix transcription factor(Foxp3) mRNA and retinoid-related orphan receptor gammat (RORγt) mRNA in lung tissue of each group were gained by Real-time PCR method.Results GSPE inhibited ovalbumin-induced increases in airway responsiveness(P ＜ 0.05).Histological studies demonstrated that GSPE substantially inhibited OVA-induced airway inflammation in lung tissue.GSPE decreased IL-17A levels and increased IL-10 levels in bronchoalveolar lavage fluid (P ＜ 0.05).In the asthma model group,RORγt mRNA expression in lung tissue was significantly higher than that in the control group(P ＜ 0.05)and Foxp3 mRNA expression was significantly lower than that in the control group (P ＜ 0.05).In the GSPE group,RORγt mRNA expression was lower than that in asthma model group (P ＜ 0.05),however the Foxp3 mRNA expression was higher than that in asthma model group(P ＜ 0.05).Conclusion GSPE could alleviate airway hyperresponsiveness and airway inflammation of in asthmatic mice.It can modify the asthmatic mice Treg/Th17 imbalance by decreasing IL-17A and increasing IL-10 concentration at the level of cytokines;and also by increasing Foxp3 mRNA expression and inhibiting the expression of RORγt mRNA at the transcriptional level,which provide a new way for treatment of bronchial asthma.

Objective To explore the effect of grape seed proanthocyanidin extract(GSPE) on the proliferation of airway smooth muscle cells( ASMCs) induced by platelet-derived growth factor( PDGF) and the underlying molecular mechanism. Methods ASMCs of primary rat were cultured. MTT and flow cytom-etry were used to detect the cell proliferation activity and cell cycle distribution of ASMCs which were treated with PDGF and GSPE respectively. The expression levels of cyclin D1,extracellular regulated protein kinases ( ERK)1/2,p-ERK1/2 and β-actin protein in each group ASMCs were analyzed using western blotting assay after ERK1/2 inhibitor PD98059 intervention. Results Compared with control group,cell proliferative activ-ity,S phase fraction and the expression of cyclin D1 and p-ERK1/2 protein increased in PDGF induced group (P<0. 05). These effects induced by PDGF could be reversed by GSPE. PD98059 also could block PDGF induced higher expression of p-ERK1/2 and cyclin D1 proteins in rat ASMCs. Conclusion GSPE can inhib-it PDGF induced cell proliferation and via ERK1/2 signaling pathway in rat ASMCs,which provide a new way for treatment of bronchial asthma.

Sleep disorder is common in patients with asthma, especially in patients with severe asthma.Sleep disorder correlates with poor asthma control and poor quality of life.Sleep disorder in asthmatic patients may be related to the circadian variation in airway physiology and airway inflammation, but may also be related to specific sleep disorders such as obstructive sleep apnea(OSA), gastroesophageal reflux, obesity, psychological problems, etc.OSA is an independent risk factor for poor asthma control.At the same time, asthma will aggravate OSA.Treatment of OSA with continuous positive airway pressure(CPAP)may lead to improved asthma-specific quality of life.

Objective To investigate the prevalence of metabolic syndrome (MS) in 259 professional automobile drivers,and to put forward targeted suggestions on protection.Methods In October 2014,114 male bus drivers and 145 male taxi drivers in a transportation service company were enrolled as investigation group,and 121 non-operating male staff were enrolled as control group.Physical examination and a questionnaire survey were conducted for both groups,and the results were analyzed.Results The bus drivers and taxi drivers had significantly higher prevalence rates of MS than the non-operating staff (17.5％/13.1％ vs 3.3％,P＜0.05).The results of univariate logistic analysis showed that smoking (OR=2.58,95％CI 1.14～5.88),exercise (OR=0.21,95％CI 0.10～0.43),meal time (OR=0.27,95％CI 0.13～0.59),and a family history of chronic diseases (OR =2.26,95％ CI 1.13～4.50) were associated with MS,and each independent variable showed significant differences between groups (P＜0.05).Multivariate logistic regression analysis showed that with age remaining the same,smoking was the risk factor for MS in professional automobile drivers (OR=5.25,95％CI 2.00～13.80),and meal time(20～40 min)(OR=0.20,95％CI 0.09～0.44)and exercise (OR=0.13,95％CI 0.06～0.30)were protective factors against MS.Conclusion Professional automobile drivers have a higher prevalence rate of MS than non-operating staff,which should be taken seriously by working personnel.

Objective To investigate the prevalence of metabolic syndrome (MS) in 259 professional automobile drivers,and to put forward targeted suggestions on protection.Methods In October 2014,114 male bus drivers and 145 male taxi drivers in a transportation service company were enrolled as investigation group,and 121 non-operating male staff were enrolled as control group.Physical examination and a questionnaire survey were conducted for both groups,and the results were analyzed.Results The bus drivers and taxi drivers had significantly higher prevalence rates of MS than the non-operating staff (17.5％/13.1％ vs 3.3％,P＜0.05).The results of univariate logistic analysis showed that smoking (OR=2.58,95％CI 1.14～5.88),exercise (OR=0.21,95％CI 0.10～0.43),meal time (OR=0.27,95％CI 0.13～0.59),and a family history of chronic diseases (OR =2.26,95％ CI 1.13～4.50) were associated with MS,and each independent variable showed significant differences between groups (P＜0.05).Multivariate logistic regression analysis showed that with age remaining the same,smoking was the risk factor for MS in professional automobile drivers (OR=5.25,95％CI 2.00～13.80),and meal time(20～40 min)(OR=0.20,95％CI 0.09～0.44)and exercise (OR=0.13,95％CI 0.06～0.30)were protective factors against MS.Conclusion Professional automobile drivers have a higher prevalence rate of MS than non-operating staff,which should be taken seriously by working personnel.

Objective:To explore the clinical characteristics of children with atopic mycoplasma pneumoniae pneumonia and to provide evidence for the diagnosis and treatment of children with atopic mycoplasma pneumoniae pneumonia.Methods:One hundred and eighty cases of children diagnosed with mycoplasma pneumoniae pneumonia in Shengjing Hospital of China Medical University from January 2018 to December 2018 were selected. According to whether they had atopic constitution, they were divided into atopic mycoplasma pneumoniae pneumonia(AMPP)group(84 cases)and non-atopic mycoplasma pneumoniae pneumonia(NAMPP)group(96 cases). The clinical data of age, sex, fever time, hospital stay, application time of macrolides, white blood cells, CRP, LDH, and lung CT were collected from the two groups, and the differences in clinical manifestations, laboratory examinations and imaging manifestations of the two groups were analyzed retrospectively.Results:(1)Both the absolute value of eosinophils and total IgE values in the AMPP group were higher than those in the NAMPP group, and the difference was statistically significant( P<0.05). The incidence of severe mycoplasma pneumoniae pneumonia(SMPP)and/or refractory mycoplasma pneumoniae pneumonia(RMPP)and chest imaging manifestations of interstitial pneumonia in the AMPP group was higher, and the difference was statistically significant( P<0.05). (2)The incidence of wheezing in the AMPP group was 48.81%(41 cases/84 cases), which was significantly higher than that in the NAMPP group 22.92%(22 cases/96 cases). The duration of cough and wheezing in the AMPP group was longer than that in the NAMPP group( P<0.05), with statistically significant differences( P<0.05). (3)In the AMPP group, 36.90%(31 cases /84 cases)of the children received intravenous methylprednisolone treatment, which was significantly higher than the 20.83%(20cases /96 cases)of the NAMPP group. Lung rales absorption time in the AMPP group[(9.73±3.59)d] was significantly longer than that in the NAMPP group[(7.52±2.44)d], and the difference was statistically significant( P<0.05). Lung CT examination showed that the absorption of lung inflammation in the AMPP group was worse than that in the NAMPP group, with a statistically significant difference( P<0.05). The hospitalization time of children in the AMPP group[(10.88±4.17)d] was longer than that in the NAMPP group[(9.68±2.68)d], with a statistically significant difference( P<0.05). Conclusion:The condition of AMPP is more serious than that of NAMPP, and it is more likely to cause incomplete absorption of pulmonary inflammation.