1.Effect of Baicalin on the Content of Major Amino Acid Transmitters in the Brain of Rat with Experimental Intracerebral Hemorrhage
Qiankun ZHOU ; Siming YU ; Ping LIU
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(04):-
Objective To study the effect of baicalin on the content of glutamate acid (Glu), aspartic acid (Asp), ?-aminobutyric acid (GABA) in the brain of rat with experimental intracerebral hemorrhage. Methods The intracerebral hemorrhage rat model was induced by collagen enzyme + heparin sodium. The rats were randomly divided into sham group, model group and high, middle, low dose group of baicalin. Rats were treated with the physiological saline and different concentration of baicalin respectively. The brain of each group was taken out three days later and the brain homogenate was prepared. The content of Glu, Asp and GABA was measured with the high performance liquid chromatography (HPLC). Results Compared with the sham group, the levels of Asp were obviously higher (P0.05) in each dose group of baicalin. Compared with the sham group, the contents of Glu were increased obviously (P
2.Neuroprotection of Baicalin on Brain Damage Following Experimental Intracerebral Hemorrhage in Rats
Ping LIU ; Yishuang WEI ; Qiankun ZHOU
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(04):-
Objective To observe the neuroprotection of Baicalin on brain damage following experimental intracerebral hemorrhage in rats.Methods Rat model of intracerebral hemorrhage was induced by collagenase method.The rats were randomly divided into sham operation control group,model group,high-,middle-and low-dose drug group,treated with different dose of Baicalin.The brain water content and the level of SOD,MDA,NO and NOS were measured at the third and seventh day after modeling repectively.Results Compared with the sham operation control group,the brain water content and the level of MDA,NO and NOS were significantly higher,and the level of SOD was significantly lower in model group.The brain water content and the level of MDA,NO and NOS were significantly lower,and the level of SOD was obviously higher in high-,middle-and low-dose Baicalin group than that in model group.Conclusion Baicalin has evident protective effect on brain damage after experimental intracerebral hemorrhage in rats,which maybe related to the increase of SOD and decrease of MDA,NO and NOS.
3.Relationship between methylenetetrahydrofolate reductase gene polymorphism and unstable angina pectoris disease
Hui YE ; Qiankun YE ; Gaixia DONG ; Xiangjing ZHOU ; Geng TIAN ; Ying LI ; Yaohui HUANG
Clinical Medicine of China 2009;25(5):480-482
Objective To explore the relationship between methylenetetra hydrofolate reduetase (MTHFR) C677T genotypo and unstable angina pectoris(UA) in Chinese population. Methods The study consisted of 90 UA cases (UA group), and an age- and sex- matched healthy control cases (control group, n = 90). PC R-RFLP was used to analyze polymorphism of the MTHFR C677T genotypo. The relationship between MTHFR C677T genotype and UA was observed. Results MTHFR 677C→T mutation was found in 30 of 90 patients with unstable angina pectoris (33.33%) and in 15 of 90 control subjects (16.67%). This difference was statistically significant (P<0.05). Conclusion MTHFR 677C→T mutation is closely related to the unstable angina poctoris.
4.Performance Investigation of Ion Trap with Various Collision gas and Pressures
Fuxing XU ; Qiankun DANG ; Hangyu DING ; Zhengxu HUANG ; Yuanyuan WANG ; Zhen ZHOU ; Chuanfan DING
Chinese Journal of Analytical Chemistry 2017;45(4):587-592
Ion trap performances are investigated based on digital ion trap technique with different collision gases at different pressures. Collision gases of helium (4 amu), nitrogen (28 amu) and argon (40 amu) with various pressures are adopted in ion excitation and dissociation stages to investigate the ion trap performances, including mass resolution, signal intensity, tandem mass spectrometric analysis ability and low-mass cut off (LMCO) effect. It is found that when heavy gas of argon is used, energy can be efficiently transferred and LMCO effect is decreased with higher ion capture and dissociation efficiencies but with low mass resolution. Higher mass resolution is realized with helium as collision gas. Furthermore, at the same gas pressure, heavy gas is beneficial to abundant fragment ions and structural information of precursor ion.
5.Lung transplantation in 42 cases:an 8-year experience in a single center
Qiankun CEHN ; Gening JIANG ; Wenxin HE ; Jiaan DING ; Xiao ZHOU ; Hao WANG ; Chang CHEN ; Yuming ZHU ; Wen GAO ; Haifeng WANG
Chinese Journal of Thoracic and Cardiovascular Surgery 2011;27(10):594-596
Objective To evaluate the complications and prognostic factors of lung transplantation performed in a single center.Methods A rettospective analysis of demographic and outcome data of lung transplantation was performed.Survival analyses were performed using Kaplan-Meier estimation.Results Between January 2003 and April 2011,42 lung transplant procedures were performed.Overall survival rate at 1,3,and 5 years were 89%,59% and 38%,respectively.1,3,and 5 years survival in patients with COPD was 83%,66% and 45%,respectively,which were better than other primary end stage lung diseases ( 78%,17% and 17%,respectively,P =0.013).Postoperative complications included pulmonary bacterium infection in 8 patients (20%),fungal infection in 12 (30%),and airway complications in4 (9.5%).35% of patients had at least 1 episode of acute rejections within the first year,and 22.5% of patients had BOS.2 patients underwent single lung retransplantation.Conclusion In this single center study,patients with COPD may have a good long-term survival.The most common postoperative complications were pulmonary infection and airway complication.
6.Tracheal sleeve pneumonectomy for bronchogenic carcinoma invading the carina
Qiankun CEHN ; Gening JIANG ; Jiaan DING ; Chang CHEN ; Xiaofeng CHEN ; Yuming ZHU ; Hao WANG ; Xiao ZHOU ; Wen GAO
Chinese Journal of Thoracic and Cardiovascular Surgery 2012;28(3):129-131
ObjectiveBronchogenic carcinoma involving the carina or tracheobronchial angle still presents a challenge due to specific problems related to surgical technique and airway management.Aim of this paper is to examine complications and long-term survival of our personal series and those reported in literature.MethodsBetween 1985 and 2010,48 patients underwent carinal resection:a right tracheal sleeve pneumoneetomy was performed in 47 patients and a left tracheal sleeve pneumonectomy in 1 patient.The anastomosis was performed with aid of high-frequency jet ventilation or introfield tube ventilation.ResultsOverall morbidity and mortality rate was 25% and 6.3% respectively,and there was no death in operation.5-yearsurvival rate of patients with squamous and adenocarcinoma was 27.3% and 12.5%,respectively,P =0.04.The overall 5-year survival rate was 24.3%.Patients without nodal involvement had a significantly better prognosis than N1 and N2 patients (5-year survival:52%,13% and 0,respectively).Multivariate analysis showed that nodal status was the only independent prognostic factor( P =0.006 ).ConclusionWith careful selection of patients and meticulous surgical technique,Tracheal sleeve pneumonectomy for bronchogenic carcinoma can be accomplished with acceptable mortality and morbidity,proriding good long-term results.
7.First successful bilateral living-donor lobar lung transplantation in China.
Qian-kun CHEN ; Ge-ning JIANG ; Jia-an DING ; Wen GAO ; Chang CHEN ; Xiao ZHOU
Chinese Medical Journal 2010;123(11):1477-1478
Child
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China
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Humans
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Living Donors
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Lung Injury
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therapy
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Lung Transplantation
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methods
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Male
9.Cultivation of college students' innovative and entrepreneurial thinking and ability based on Synthetic Biology and iGEM.
Tiantian JIAN ; Suiyan LI ; Hai LIAO ; Jiayu ZHOU ; Qiankun ZHU ; Xinhe HUANG
Chinese Journal of Biotechnology 2022;38(4):1619-1630
Synthetic Biology is one of the most promising fields of modern Biology and a frontier interdisciplinary subject in the 21st century. With the rapid development of synthetic biology, the International Genetically Engineered Machine (iGEM) competition has emerged. The iGEM competition, based on the subject foundation of Synthetic Biology, intends to solve the biological problems in our daily life by applying modern biological technology. In recent years, with the continuous increase of participating teams, the iGEM competition has received extensive attention and achieved great progress. On the basis of the development of Synthetic Biology, we analyzed the 2018-2020 award-winning projects of the iGEM competition and illustrated the role and significance of the iGEM competition in cultivating college students' innovative thinking and ability with the participation experience of the iGEM team of Southwest Jiaotong University as an example.
Genetic Engineering
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Humans
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Students
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Synthetic Biology
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Universities
10.Shanghai Pulmonary Hospital Experts Consensus on the Management of Ground-Glass Nodules Suspected as Lung Adenocarcinoma (Version 1).
Gening JIANG ; Chang CHEN ; Yuming ZHU ; Dong XIE ; Jie DAI ; Kaiqi JIN ; Yingran SHEN ; Haifeng WANG ; Hui LI ; Lanjun ZHANG ; Shugeng GAO ; Keneng CHEN ; Lei ZHANG ; Xiao ZHOU ; Jingyun SHI ; Hao WANG ; Boxiong XIE ; Lei JIANG ; Jiang FAN ; Deping ZHAO ; Qiankun CHEN ; Liang DUAN ; Wenxin HE ; Yiming ZHOU ; Hongcheng LIU ; Xiaogang ZHAO ; Peng ZHANG ; Xiong QIN
Chinese Journal of Lung Cancer 2018;21(3):147-159
Background and objective As computed tomography (CT) screening for lung cancer becomes more common in China, so too does detection of pulmonary ground-glass nodules (GGNs). Although anumber of national or international guidelines about pulmonary GGNs have been published,most of these guidelines are produced by respiratory, oncology or radiology physicians, who might not fully understand the progress of modern minimal invasive thoracic surgery, and these current guidelines may overlook or underestimate the value of thoracic surgery in the management of pulmonary GGNs. In addition, the management for pre-invasive adenocarcinoma is still controversial. Based onthe available literature and experience from Shanghai Pulmonary Hospital, we composed this consensus about diagnosis and treatment of pulmonary GGNs. For lesions which are considered as adenocarcinoma in situ, chest thin layer CT scan follow-up is recommended and resection can only be adopt in some specific cases and excision should not exceed single segment resection. For lesions which are considered as minimal invasive adenocarcinoma, limited pulmonary resection or lobectomy is recommended. For lesions which are considered as early stage invasive adenocarcinoma, pulmonary resection is recommend and optimal surgical methods depend on whether ground glass component exist, location, volume and number of the lesions and physical status of patients. Principle of management of multiple pulmonary nodules is that primary lesions should be handled with priority, with secondary lesions taking into account.
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Adenocarcinoma
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diagnosis
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diagnostic imaging
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surgery
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Adenocarcinoma of Lung
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China
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Consensus
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Hospitals
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Humans
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Lung Neoplasms
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diagnosis
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diagnostic imaging
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surgery
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Physicians
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psychology
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Positron Emission Tomography Computed Tomography
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Practice Guidelines as Topic
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Retrospective Studies
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Solitary Pulmonary Nodule
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diagnosis
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diagnostic imaging
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surgery
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Tomography, X-Ray Computed