1.Neuroprotection of Baicalin on Brain Damage Following Experimental Intracerebral Hemorrhage in Rats
Ping LIU ; Yishuang WEI ; Qiankun ZHOU
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(04):-
Objective To observe the neuroprotection of Baicalin on brain damage following experimental intracerebral hemorrhage in rats.Methods Rat model of intracerebral hemorrhage was induced by collagenase method.The rats were randomly divided into sham operation control group,model group,high-,middle-and low-dose drug group,treated with different dose of Baicalin.The brain water content and the level of SOD,MDA,NO and NOS were measured at the third and seventh day after modeling repectively.Results Compared with the sham operation control group,the brain water content and the level of MDA,NO and NOS were significantly higher,and the level of SOD was significantly lower in model group.The brain water content and the level of MDA,NO and NOS were significantly lower,and the level of SOD was obviously higher in high-,middle-and low-dose Baicalin group than that in model group.Conclusion Baicalin has evident protective effect on brain damage after experimental intracerebral hemorrhage in rats,which maybe related to the increase of SOD and decrease of MDA,NO and NOS.
2.Effect of Baicalin on the Content of Major Amino Acid Transmitters in the Brain of Rat with Experimental Intracerebral Hemorrhage
Qiankun ZHOU ; Siming YU ; Ping LIU
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(04):-
Objective To study the effect of baicalin on the content of glutamate acid (Glu), aspartic acid (Asp), ?-aminobutyric acid (GABA) in the brain of rat with experimental intracerebral hemorrhage. Methods The intracerebral hemorrhage rat model was induced by collagen enzyme + heparin sodium. The rats were randomly divided into sham group, model group and high, middle, low dose group of baicalin. Rats were treated with the physiological saline and different concentration of baicalin respectively. The brain of each group was taken out three days later and the brain homogenate was prepared. The content of Glu, Asp and GABA was measured with the high performance liquid chromatography (HPLC). Results Compared with the sham group, the levels of Asp were obviously higher (P0.05) in each dose group of baicalin. Compared with the sham group, the contents of Glu were increased obviously (P
3.Effects of gensenoside Rg1 on tau protein phosphorylation induced by okadaic acid in rat brain slices.
Xi LI ; Ying LIU ; Haifeng YUAN ; Qiankun QUAN
Journal of Integrative Medicine 2010;8(10):955-60
To investigate the effects of gensenoside Rg1 on expressions of phosphorylated tau protein (P-tau), protein phosphatase 2A (PP2A) and tau protein in Alzheimer's disease-like tau phosphorylation rat brain slices, and to explore the mechanisms of gensenoside Rg1 in inhibiting tau phosphorylation.
4.The establishment and evaluation of food allergy model in SD young rats
Junhong LIU ; Bin WU ; Qiankun LAI ; Chun LIN
Journal of Clinical Pediatrics 2013;(10):953-958
Objectives To explore suitable conditions for establishing food allergy model through sensitization by in-traperitoneal injection (i.p) with low-dose ovalbumin (OVA) and challenge by gavage with high-dose OVA in SD young rats, and to evaluate the model. Methods Sixteen three-week-old female SD young rats were randomly divided into two groups with 8 rats each. SD young rats in food allergy (FA) group were ifrst sensitized by intraperitoneal injection with 0.2 ml suspension mixed with 40 μg OVA and 1mg Al(OH)3 on the ifrst day (d 0), then intraperitoneally injected with 0.2 ml (40 μg) OVA solution on days 2, 4, 7, 9 and 11, and lastly challenged by gavage with 2.0 ml (15 mg/ml) OVA solution on days 20, 24, 28 and 30. The rats in the control group were intraperitoneally injected and gavaged with the same volume of normal saline instead of OVA during the same period. The eosinophils (EOS), mast cells (MC), the integrity of MC in intestinal mucosa of two groups were observed, and ovalbumin speciifc IgE (OVA-IgE) levels in serum were analyzed. Results The rats in FA group had lusterless hair and diarrhea, and compared with control group, OVA-IgE levels were increased signiifcantly (P<0.05). Compared with control group, the intestinal mucosa of jejunum, ileum and colon in FA group had more damage, with more EOS and degranu-lated MC aggregated (P<0.01). Conclusions The allergy model established through sensitization by intraperitoneal injection with low-dose OVA mixed with adjuvant Al(OH)3 and challenge by gavage with high-dose OVA in young rats had clinical features and intestinal pathological changes consistent with food allergy infants and it was an ideal food allergy model in SD young rats.
5.The value of peripheral blood neutrophil CD64 expression in early differential diagnosis of etiology in children with community-acquired pneumonia
Guanghua LIU ; Qiankun LAI ; Hong YE ; Shibiao WANG ; Xiaodan MAO
Chinese Pediatric Emergency Medicine 2016;23(7):476-479
Objective To evaluate the apphcation value of peripheral blood neutrophil CD64 expression in early differential diagnosis of etiology in children with community-acquired pneumonia.Methods From June 2014 to June 2015 in our hospital,total of 99 cases of different pathogen infection were divided into three groups,bacterial pneumonia group (n =41),non-bacterial pneumonia group(n =38),healthy children group (n =20);and the non-bacterial pneumonia group was divided into two subgroups,viral pneumonia group (n =22) and mycoplasma pneumoniae pneumonia group(n =16).Flow cytometry was used to test the median fluorescence intensity (MFI) of peripheral blood neutrophil CD64.The levels of peripheral blood CRP,WBC and neutrophil percentage were detected.Results The levels of CD64 expression,CRP and WBC in bacterial pneumonia group were statistically higher than those of non-bacterial pneumonia group and healthy children group respectively (both P < 0.01).The optimal cutoff value of CD64 was 6 519 MFI when using ROC curve,and the sensitivity and specificity of the diagnosis of bacterial pneumonia were 87.8% and 89.7% respectively.The levels of CD64 expression in bacterial pneumonia group were significantly higher than those in viral pneumonia group,mycoplasma pneumoniae pneumonia group and healthy children group (all P < 0.01).Conclusion The expression of peripheral blood neutrophil CD64 increases in children with community-acquired pneumonia of bacteria infection.It can be used to guide early diagnosis of children with bacterial pneumonia and the using of antibiotics.
6.The effects of Saccharomyces boulardii powder on preventing antibiotic-associated diarrhea in children with pneumonia
Hong YE ; Qiankun LAI ; Guanghua LIU ; Lumin CHEN ; Zijing WANG
International Journal of Pediatrics 2013;40(4):423-425
Objective The aim of this randomized,double-blind,placebo-controlled trial was to evaluate the efficacy of the Saccharomyces boulardii (S.boulardii) power in preventing antibiotic-associated diarrhea (AAD) in hospitalized children with pneumonia.Methods Two hundred and forty-five hospitalized children with pneumonia were randomly divided into two groups,group 1 treated with antibiotics and S.boulardii power (n =104),and group 2 with antibiotics and placebo,along with S.boulardii power and zinc when suffered from diarrhea (n =141).All children received montmorillonite powder and fluid replacement therapy for diarrhea.The stool frequency and consistency were observed.Diarrhea incidence,diarrhea and pneumonia duration of treatment,and therapeutic effect were recorded.Results AAD is more prevalent in group 2 than in group 1 (P <0.05).A 55.7% relative risk reduction in AAD was seen in group 1 compared with group 2 (RR =0.443,95%CI 0.233 ~ 0.845,P =0.012).Patients with preventive treatment of S.boulardii power in broup 1 had lower AAD incidence compared with those in group 2 (P < 0.05).Both the diarrhea and pneumonia duration of treatment in group 1 were shorter than that in group 2(P =0.001 for diarrhea duration,P =0.030 for pneumonia duration,respectively).No drug related adverse reactions were found during the observation period.Conclusion Preventive use of S.boulardii power may effectively reduce the risk of AAD,improve therapeutic effect,and shorten hospital stays for hospitalized children with pneumonia.
7.Investigation of quality of life of recovered patients with depression and its influential factors
Qiankun YAO ; Hong YANG ; Yan REN ; Xuemei LIAO ; Qian MENG ; Yiping LIANG ; Xia LIU ; Kerang ZHANG
Chinese Journal of Behavioral Medicine and Brain Science 2010;19(2):127-129
Objective To investigate the health-related quality of life(HRQOL)of the recovered patients with depression and its influential factors.Methods From March 2008 to April 2009,150 depressive patients andergoing the following up treatment were recruited,and finally 124 patients rocovered,and quality of life for patients recovered were compared to published norms for the general Chinese people.The general demography material seale.Hamilton Depression Scale(HAMD),Hamilton Anxiety Scale(HAMA),personality traits(Eysenck personality Inventory-EQP)the short form 36 item(SF-36)questionnaire and social support scale(SSS)were applied to an subjects.The SPSS 13.0 software for data processing,the group t test and the multiple linear regression analysis was adopted.Results ①The average score of seven domains of quality of life(RP,BP,GH,VT,SF,RE,MH )for the recovered patients were better than that of the pre-treatment patients(P<0.01),except the PF(89.23±14.09,87.23±16.81,t=1.044,P>0.05).But the average score of seven domains of quality of life for the re-covered patients were worse than that of the healthy people(P<0.01),except the PF(89.23±14.09,89.01±15.73,t=1.266,P>0.05).②The quality of life 0f the recovered patients remarkably related with the anxiety level, neuroticism, extraversion, recurrent depression,economic condition and social support(P<0. 01) . Conclusion The recovered depressive patient s health-related quality of life were improved significantly afer treatment, what were influenced by neuroticism,extraversion,anxiety as a trait,recurrent depression,economic condition and social support.Above mentioned factors should be taken into account when rehabilitation.
8.Clinical effectiveness of SVF gel in periorbital accurate injection
Chinese Journal of Medical Aesthetics and Cosmetology 2019;25(1):39-41
Objective To introduce the SVF gel as a filling,and the application of SVF gel in periorbtal accurate injection.Methods This investigation presented a retrospective study of 35 patients in Henan Aeasthetic and Plastic Hospital from December 2016 to March 2018.We used the SVF gel for periorbital accurate injection for the 35 patients.The effectiveness was observed 3 to 6 months later.Patient satisfaction was evaluated with the Likert scale.Results All cases were obviously improved.All of them were satisfied with the outcomes.No complication occurred,All the swelling time lasts 5-8 days.Conclusions With smaller granules and higher survival rate,the technique of SVF gel can achieve satisfactory results in accurately periorbital injection.
9.Prevention of bronchopulmonary complications by ambroxol after thoracotomy in patients with chronic obstructive pulmonary disease
Xingfeng ZHU ; Mingfeng ZHENG ; Jingyu CHEN ; Yijun HE ; Ruo CHEN ; Qiankun ZHU ; Shugao YE ; Feng LIU ; Rongguo LU
Chinese Journal of Geriatrics 2011;30(12):1000-1002
Objective To evaluate the effects of ambroxol on prevention of bronchopulmonary complications after thoracotomy in patients with chronic obstructive pulmonary disease(COPD).MethodsTotal 161 elderly patients aged ≥70 years with COPD undergoing thoracotomy were randomly allocated to two groups:ambroxol group (300 mg/d,6 d) and placebo group as control.Pulmonary complications were evaluated by clinical symptoms,radiographic changes,and blood gas analysis. Results The incidence rates of atelectasis were 8.6% in ambroxol group and 28.8% in placebo group,respectively.The PaO2 values after surgery in ambroxol group decreased more than in placebo group (P<0.05) compared with the preoperative values.Side effects were not found in all patients.Conclusions Ambroxol should be considered as an alternative pharmacologic approach for the prevention of post-thoracotomy pulmonary complications in the elderly patients with COPD.
10.Effect of ginsenoside Rg1 on expressions of phosphory protein tau and N-methyl-D-aspartate receptor subunits NR1 and NR2B in rat brain slice model of Alzheimer's disease.
Xi LI ; Ying LIU ; Xin ZHANG ; Haifeng YUAN ; Qiankun QUAN
China Journal of Chinese Materia Medica 2010;35(24):3339-3343
OBJECTIVETo investigate the effect of ginsenoside Rg1 on the expressions of phosphory protein Tau (P-Tau), N-methyl-D-aspartate receptor subunit 1 (NR1) and N-methyl-D-aspartate receptor subunit 2B(NR2B) in rat brain slice model of Alzheimer's disease.
METHODBrains of 5-week-old Wistar rats were cut into slices which were 400 microm thick. These brain slices were randomly divided into normal control group, untreated group, low-dose ginsenoside Rg1 group, medium-dose ginsenoside Rg1 group and high-dose ginsenoside Rg1 group, with 10 slices in each group. All brain slices were cultured with artificial cerebrospinal fluid (ACSF) that was aerated via polyethylene tubing attached to a source of 95% O2, 5% CO2 at (32.0 +/- 0.5) degrees C. And brain slices in the ginsenoside R1 groups were administrated with the ginsenoside Rg1 (60, 120 and 240 micromol x L(-1) respectively) in ACSF for 2 h firstly. Then okadaic acid (OA) was administrated into ACSF of untreated group and ginsenoside Rg1 groups separately for 3 h to induce Tau phosphorylation to prepare AD models. The concentration of OA in each group was 1 micromol x L(-1). And there was no any intervention for the brain slices in the normal control group. The expressions of P-Tau, NR1 and NR2B in brain slices in each group were determined by immunohistochemical method, and the results were analyzed by image acquisition and analysis system.
RESULTCompared with the normal control group, the expression of P-Tau was significantly increased (P < 0.05 or P < 0.01) and the expressions of NR1 and NR2B were decreased (P < 0.01) in untreated group. Compared with the untreated group, the expression of P-Tau was significantly decreased (P < 0.01 or P < 0.05) and the expressions of NR1 and NR2B were increased (P < 0.01 or P < 0.05) in ginsenoside Rg1 groups, especially in high-dose ginsenoside Rg1 group.
CONCLUSIONGinsenoside Rg1 can play the role of anti-dementia by inhibiting the expression of P-Tau so as to slow the formation of neurofibrillary tangles and increasing the expression of NR1 and NR2B so as to improve learning and memory abilities in rat brain slice model of Alzheimer's disease.
Alzheimer Disease ; metabolism ; pathology ; Animals ; Brain ; drug effects ; metabolism ; pathology ; Disease Models, Animal ; Gene Expression Regulation ; drug effects ; Ginsenosides ; pharmacology ; Hippocampus ; drug effects ; metabolism ; pathology ; In Vitro Techniques ; Male ; Phosphoproteins ; metabolism ; Rats ; Rats, Wistar ; Receptors, N-Methyl-D-Aspartate ; metabolism ; tau Proteins ; metabolism