Objective To study the effects of rAAV2/1-Acrp30 on sICAM-1 and sVCAM-1 level as well as NF-?B expression in GK rats with diabetic arteriosclerosis so as to explore the effect of adiponectin on diabetic macroangiopathy. Methods A total number of 30 atherosclerotic GK rat models were randomly divided into three groups:① Model group one (M1):hind limb intramuscular injection of normal saline; ② Model group two (M2):hind limb intramuscular injection of vacuity virus rAAV2/1; ③ Treatment group (T):hind limb intramuscular injection of rAAV2/1-Acrp30 at a dose of 1?1012mg/L. After 8 weeks' treatment,the rats were killed,and serum sVCAM-1 and sICAM-1 level as well as aortic NF-?Bp65mRNA expression were measured in each group. Aortic NF-?Bp65mRNA expression was measured by RT-PCR.Results Compared with those in control model groups (M1 and M2),sVCAM-1 and sICAM-1 levels were decreased significantly in the treatment (T) group (P

BACKGROUND: Peritonitis is the common detective complications at the end-stage of renal failure patients during continuous ambulatory peritoneal dialysis (CAPD) treatment, which easily repeatedly occurred if not handled properly. Although the plumbing designs of peritoneal dialysis have done a lot of effort and improved in recent years, it still hard to avoid. OBJECTIVE: To explore the relative factors and treatments of infectious peritonitis complicated with CAPD. METHODS: Totally 115 cases received CAPD were selected, including 55 males and 60 females, aged (56.2?.7) years. 68 cases of which were suffered infectious peritonitis, aged (58.5?.3) years. All received a (30.3?.5)-month CAPD treatment. The relative factors such as the nutrition situation, whether complicated with diabetes mellitus or not, culture background, domestic environment, whether operating by special person and so on were investigated, and the relationship between those factors and infectious peritonitis were analyzed. The pathogen method was used to check pathogenic bacteria, and those patients were treated with irrigation of abdominal cavity and antibiotics in two ways, that is, systemic and intra-abdominalcavity. Hemodialysis instead of CAPD was used in those infectious peritonitis patients.RESULTS AND CONCLUSION: Those patients with good nutrition situation, higher culture background, better domestic environment, and operating by special person had low chances to infect peritonitis. And those patients with diabetes mellitus had high chances to infect peritonitis. Treatments of abdominal cavity and anti-infection in two ways-systemic and intra-abdominal cavities were effective to those infectious peritonitis patients. Gram-positive bacteria were the most common pathogen, which were susceptive to vancomycin and cefazolin. If the pathogen was gram-negative bacteria, etimicin sulfate, amikacin, and imipenem cilastatin could be selected. Infectious peritonitis complicated with CAPD affected by many factors. CAPD patients should be provided better nutrition and domestic situation. Also patients should operate in aseptic ways. Doctors should choose proper patients who can operate CAPD right.

OBJECTIVE: To observe the effects of aminoguanidine and vitamin C on serum laminin of rats with diabetic nephropathy model. METHODS: The rats were randomly divided into normal control group, diabetes model group, vitamin C group,aminoguanidine group,vitamin C plus aminogucinidine group. The diabetes model(Type 1) was established in all groups except the normal control group by injecting them intraperitoneally with streptozotocin, after which, they were treated with the corresponding drugs for 16 weeks. During and after the treatment, the general state, blood gloucose levels, glycosylated hemoglobin, serum laminin, urea nitrogen, serum creatinine, and the 24-hours urinary albumin excretion rate of each group were observed and determined. RESULTS: The serum laminin levels increased and diabetic nephropathy occurred in all the 4 groups after being modelled. Aminoguanidine group, vitamin C had no effect on blood glucose level, while they could improve the basic condition and the combination of the two could significantly decrease serum levels of laminin, urea nitrogen, creatinine and the 24-hours urinary albumin excretion rate. CONCLUSION: Aminogucinidine and vitamin C couldn't decrease the blood sugar level but the combination of which has a synergistic action to decrease serum laminin level while preventing renal function.

Objective To clear, amplify and detect the activity of the recombinant adeno-assoeiated virus vector with adiponectin( rAAV2/1-Aerp30 ). Methods Recombinant plasmid pSNAV2.0-Acrp30 was obtained. The recombinant plasmid was then transfected into BHK21 cells using LipofectAMINETM 2000. The G418 resistant cells were obtained consequently. These cells were infected with HSVI-rc/△UL2 which has the function of packaging and copying recombinant AAV. After purification, the construction of recombinant rAAV2/1-Aerp30 was collected. Results The construction of recombinant pSNAV2.0-Acrp30 was confirmed by PCR electrophoresis and digestion with restriction enzyme. The gene sequencing showed that the recombinant pSNAV2.0-Acrp30 was correct. The virus titer was about 1.0×1012 μg/ml. The purity f the recombinant AAV2/1 was fairly high using the SDS-PAGE method. Conclusion With this method, rAAV2/-Aerp30 with high virus titers and purity can be acquired successfully and it can meet the demands of the experimental study of Acrp30 gene therapy of GK rats.

Objective To observe the effect of puerarin on the ICAM-1 gene expression in streptozotocin-Induced diabetic nephropathy rats. Methods Diabetic nephropathy rats were induced by intraperitoneal injection of STZ.Rats were allocated into normal control group,model group,and puerarin group.The puerarin treatment lasted for 16 weeks.During and after the treatment,the general health conditions,blood glucose levels,blood urea nitrogen,serum creatinine,urinary albumin excretion rate of 24-hours,and clearance rate of creatinine of the rats were observed.The ICAM-1mRNA expressions were also determined by in situ hybridization analysis in the kidney tissue of the rats.Results Diabetes mellitus and renal function lesion occurred in the model group and puerarin group.Puerarin could improve the general health of the rats,decrease blood urea nitrogen,serum creatinine,and urinary albumin excretion rate of 24-hours;and increase the clearance rate of creatinine.The ICAM-1mRNA expressions in the kidney were significantly inhibited by puerarin treatment.Conclusion Puerarin has a function of renal protection.

Objective To observe the effects of aminoguanidine and Vitamin C on lipid of streptozotocin-induced didbetic rats.Methods Diabetic nephropathy rats were induced by intraperitoneal injection of streptozotocin.Fifty Sprague-Dawley(SD) rats were randomly divided into normal control group,model group,Vitamin C group,aminoguanidine group and Vitamin C associated with aminoguanidine group.All the rats were treated for 16 weeks.During and after the treatment,the general state of the rats was observed and the levels of blood sugar,triglyceride,total cholesterol,low density lipoprotein,high density lipoprotein,glycosylated hemoglobin,and glycated low density lipoprotein in different groups were detected.Results The rats in the four groups suffered diabetes mellitus and renal function lesion.Aminoguanidine and Vitamin C could improve the general state of the rats,but had no effect on the blood sugar.Vitamin C could decrease the levels of triglyceride,serum cholesterol,low density lipoprotein,glycosylated hemoglobin,and glycated low density lipoprotein,and increase the level of high density lipoprotein.There was a synergistic effect with the combination of the two drugs. Conclusion Aminoguanidine and Vitamin C have no effect in the decrease of blood sugar level but have a certain effect on regulating blood lipid.There is a synergistic effect with the combination of the two drugs.

AIM: To observe the effects of puerarin on blood lipid and expression of aorta laminin B_1 mRNA in streptozotocin-induced diabetic rats. METHODS: Diabetic nephropathy rats were induced by intraperitoneal injection of STZ, and the experimental rats were divided into normal control group, model group, and puerarin group. During and after the treatment for 12 weeks, the general state, blood suger(BS), triglyceride(TC), cholesterol, low density lipoprotein(LDL-C), high density lipoprotein(HDL-C), glycosylated hemoglobin(HbA1c) and glycosylated low density lipoprotein(G-LDL) were detected. Aorta alteration of tissue morphology was observed by H.E staining, and the expressions of laminin B1 mRNA were determined by in situ hybridization analysis. RESULTS: Diabetes mellitus and aorta lesion occurred in the two model groups. Puerarin could improve the general state, decrease the level of triglyceride(P

0.05). The blood glucose, glycosylated hemoglobin and oxidized low density lipoprotein degrade, insulin resistance were improved in probucol group after treatment, while the adiponectin level was increased(P