Objective To evaluate the clinical performance of high-risk human papillomavirus ( HR-HPV) E6/E7 mRNA test in female in different groups with atypical squamous cell of undetermined significance ( ASC-US) cytology.Methods 320 samples from female patients with ASC-US were detected with HR-HPV E6/E7 mRNA test and HR-HPV DNA test simultaneously, patients were examined with colposcopy and cervical biopsy.They were divided into two groups:younger than 30 years and 30 years and older.The statistical analysis were performed.Results There were no statistically significance in the positive rate of HR-HPV E6/E7 mRNA between two groups ( P>0.05) .In the group aged younger than 30 years, the positive rate of HR-HPV DNA was significantly higher than the group aged 30 years and older ( P<0.05),but the prevalence of CINⅡor greater was significantly lower than the other group (P<0.05).In the group aged younger than 30 years, the specificity of HR-HPV E6/E7 mRNA test for detection of CINⅡor greater was 68.0%,which was significantly higher (P<0.05) than that of HR-HPV DNA test 38.7%, meanwhile, the specificity of HR-HPV E6/E7mRNA test for detection of CINⅡor greater was significantly higher ( P<0.05) than that of HR-HPV DNA test ( 76.5% vs 69.4%) in the group aged 30 years and older.There were no statistically significance (P>0.05) in sensitivity, positive predictive value(PPV) and negative predictive value( NPV) between the two tests in each group.Conclusion Comparing to HR-HPV DNA test, HR-HPV E6/E7mRNA test is more suitable for triage of ASC-US including female patients who were younger than 30 years.