1. Effects of developmental exposure to DEHP on learning and memory of mice
Daji WU ; Binbin LUO ; Qiangwei FENG ; Qunan WANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2017;35(4):241-245
Objective:
To investigate the effects of developmental exposure to DEHP on learning and memory of mice.
Methods:
Male littermates of ICR mice randomly assigned to five experimental groups (
2. Experimental study on DEHP affect the neurodevelopment through interfering with placental thyroid hormones transport
Binbin LUO ; Qiangwei FENG ; Daji WU ; Qunan WANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2018;36(3):179-183
Objective:
The present study was represented by di-(2-ethylhexyl) phthalate (DEHP), to explore the role of thyroid hormones (THs) disruption in the connection of placenta and neurodevelopmental toxicity.
Methods:
During fetal mice neural tube closed (pregnancy 9.5 days, E9.5d) to begin synthesis of THs (E15.5 d), all pregnant mice were administered with different concentration of DEHP (0、10、50、200 mg/kg) by gavage once a day(10 mice per group). All pregnant mice were conducted with BrdU administration in E14d by subcutaneous injection. Seven pregnant mice from each group were scarified after anesthesia in E15.5 d, serum and amniotic fluid were collected to determinate the levels of THs(T3, T4, FT3 and FT4) by the automatic biochemical analyzer, detecting fetal mice placental protein expression of monocarboxylate transporter 8 (MCT8), organic anion transporting polypeptide 1C1 (OATP1C1) and deiodinaseⅡ&Ⅲ (DIO2, DIO3) by Western blot. Each group of the remaining three pregnant mices were killed after anesthesia in E18d, take the male fetal brain, BrdU immunohistochemistry was used to detect the proliferation and migration of fetal brain cortical neurons.
Results:
There was no abnormalities in diet, water intake, body weight and general activity of pregnant mice in each treatment group, and there were no difference in the general physiolo. Results There was no abnormalities in diet, water intake, body weight and general activity of pregnant mice in each treatment group, and there were no difference in the general physiological development status of body weight, brain weight, brain body ratio between the mice of each group. There was no statistically significant differences in serum T3, T4, FT3, FT4 and amniotic fluid FT4 in pregnant mice of each group (