OBJECTIVETo establish a high performance liquid chromatography method for the determination of tetrahydropulmatine content in treatment of winter's diseases in summer path.
METHODThe analysis was performed on the Hypersil ODS2 column (4.6 mm x 250 mm, 5 microm) with methanol and 0.1% phosphoric acid solution (adjusted pH to 6.28 with triethylamine) as mobile phase at 63:37. The flow rate was 1.0 mL x min(-1) and the UV detection wavelength was 280 nm.
RESULTThe linear range of tetrahydropulmatine was obtained from the range of 2.08 - 49.92 mg x L(-1) (r = 0.999 8). The average recovery was 97.85% with RSD 2.49%.
CONCLUSIONThis method is simple, reliable and can be used for the determination of tetrahydropulmatine in this preparation.
Chromatography, High Pressure Liquid
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methods
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Drugs, Chinese Herbal
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chemistry
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Reproducibility of Results