OBJECTIVETo investigate the nerve growth factor (NGF) regulating the expression of calcitonin gene-related peptide (CGRP) in promoting the proliferation of osteoblast-like cell (MG-63) and thus illustrate the mechanism of the NGF in wound healing.

METHODSDifferent concentrations of NGF were used to stimulate MG-63. The expression of CGRP was detected by real-time quantitative polymerase chain reaction (RT-QPCR) and enzyme-linked immunosorbent assay after 1, 2, 3, and 4 days. The proliferation of MG-63 was detected by cell counting kit-8 (CCK-8). The expression of CGRP mRNA and the proliferation of MG-63 were then detected by RT-QPCR and CCK-8 after adding the NGF receptor blocker.

RESULTSCompared with the blank control group, the expression of CGRP significantly increased by stimulating the NGF. The expression of CGRP was positively related to the concentration of NGF (P<0.05). Moreover, the expression of CGRP increased by prolonging the NGF stimulation time. The proliferation of MG-63 increased after stimulating the NGF (P<0.05). After adding the NGF receptor blocker, the expression of CGRP and the proliferation of MG-63 correspondingly decreased (P<0.05).

CONCLUSIONNGF can up-regulate the expression of CGRP and increase the proliferation of MG-63. Therefore, NGF plays a significant role in wound healing.

Animals ; Calcitonin ; Calcitonin Gene-Related Peptide ; metabolism ; Cell Line ; Ganglia, Spinal ; Humans ; Nerve Growth Factor ; metabolism ; Rats, Sprague-Dawley ; Receptor, Nerve Growth Factor ; Signal Transduction ; Up-Regulation

Cross-Sectional Studies ; Dust ; Humans ; Occupational Exposure ; adverse effects ; Pneumoconiosis ; epidemiology ; Prevalence

ObjectiveTo evaluate the application of multi-slice CT (MSCT) perfusion scan technique in predicting renal function recovery after unilateral hydronephrosis treatment.MethodsThirtyeight patients with unilateral obstructive hydronephrosis not shown on intravenous urography (IVU) and a normal contralateral kidney were recruited for this study.Patients were divided into detected (D) and undetected (UD) groups depending on whether the IVU detected urinary tract obstruction.All patients underwent plain abdominal X-ray,gray-scale ultrasonography,excretory urography and MSCT perfusion scan before and after the treatment.Patients were followed-up at six months or more after the treatment for a mean duration of 12.5 months (range from 6 to 22 ).ResultsOf the 38 cases,22 cases were in group D,16 cases were in group UD.On MSCT,renal cortex blood flow (BF) and blood volume ( BV ) value after treatment in group D were 561.1 ± 165.4 ml/( 100 g · min) and 35.9 ± 11.3 ml/100 g compared with before treatment rates of 361.6 ±109.7 ml/(100g· min) and24.1 ±10.2 ml/100g,t=-3.38,-2.34,P＜0.01,0.05.In the UD group,the differences of these parameters were after treatment 38.7 ± 15.4 ml/(100 g · min),10.306 ± 4.925 ml/100 g and before treatment 39.1 ± 22.5 ml/( 100 g · min) and 8.7 ± 4.4 ml/100 g,P ＞ 0.05.In the aspects of BF and BV,there were statistically significant differences between group D and group U D both before and after the treatment,t=9.09,4.15,P ＜ 0.01.ConclusionsM SCT perfusion can provide a valuable prediction technique of the renal function recovery in patients with unilateral obstructive hydronephrosis.Improvement of renal function can be expected after relief of obstructive hydronephrosis if the patients have a BF 361.6 ml/( 100 g · min) and BV 24.1 ml/100 g or greater measured by MSCT perfusion.

ObjectiveTo?investigate?the?nerve?growth?factor?(NGF)?regulating?the?expression?of?calcitonin?gene-related?peptide?(CGRP)?in?promoting?the?proliferation?of?osteoblast-like?cell?(MG-63)?and?thus?illustrate?the?mechanism?of?the?NGF?in?wound?healing.?Methods???Different?concentrations?of?NGF?were?used?to?stimulate?MG-63.?The?expression?of?CGRP?was?detected?by?real-time?quantitative?polymerase?chain?reaction?(RT-QPCR)?and?enzyme-linked?immunosorbent?assay?after?1,?2,?3,?and?4?days.?The?proliferation?of?MG-63?was?detected?by?cell?counting?kit-8?(CCK-8).?The?expression?of?CGRP?mRNA?and?the?proliferation?of?MG-63?were?then?detected?by?RT-QPCR?and?CCK-8?after?adding?the?NGF?receptor?blocker.?Results???Com-pared?with?the?blank?control?group,?the?expression?of?CGRP?significantly?increased?by?stimulating?the?NGF.?The?expression?of?CGRP?was?positively?related?to?the?concentration?of?NGF?(P<0.05).?Moreover,?the?expression?of?CGRP?increased?by?pro-longing?the?NGF?stimulation?time.?The?proliferation?of?MG-63?increased?after?stimulating?the?NGF?(P<0.05).?After?adding?the?NGF?receptor?blocker,?the?expression?of?CGRP?and?the?proliferation?of?MG-63?correspondingly?decreased?(P<0.05).?Conclusion???NGF?can?up-regulate?the?expression?of?CGRP?and?increase?the?proliferation?of?MG-63.?Therefore,?NGF?plays?a?significant?role?in?wound?healing.

Humans ; Aged ; Acute Kidney Injury ; Risk Factors ; Creatinine