Professor XIE Qiang's Tihu Guanding needling method, a kind of acupuncture method which takes acupoints of the Conception Vessel as the primary and acupoints of the Governor Vessel as the secondary. Acupoints Lianquan (CV 23), Tiantu (CV 22), Qihai (CV 6), Zhongwan (CV 12), Baihui (GV 20) and Dazhui (GV 14) are adopted as the basic ones. Other points can be added according to various symptoms, for instance, Yingxiang (LI 20) and Yintang (GV 29) for rhinopathy, Tinggong (SI 19) and Yifeng (TE 17) for otopathy, Yan'an (Professor XIE's experience) and Shanglianquan (EX-HN 21) for pharyngopathy and Kaiyin 1 (Professor XIE's experience) and Kaiyin 2 (Professor XIE's experience) for laryngopathy. During the needle retention, rotation manipulation should be done every 5 min at Lianquan (CV 23). And the patient should be told to put the tip of one's tongue at the the palate as well as to do deep breathing to communicate the Conception Vessel and the Governor Vessel. Moxibustion is adopted at Yongquan (KI 1) to induce the up floating fire to mingmen (where the primary yang is stored). The therapeutic effect on treatment of persistent otorhinolaryngologic diseases with the above mentioned method is approve to be good.
Acupuncture Points ; Acupuncture Therapy ; instrumentation ; methods ; Adult ; Female ; Humans ; Male ; Middle Aged ; Otorhinolaryngologic Diseases ; therapy ; Yang Deficiency ; therapy

Objective:To determine the changes of immune-associated molecules in serum of patients infected with respiratory syncytial virus subtype A (RSV-A).Methods:Serum specimens were collected from patients with respiratory infection at Department of Pediatrics of NO. 2 Clinical Teaching Hospital of Chengde Medical University during August 2022 to June 2023. The serum specimens were set as RSV-A single infection group and RSV-A coinfection group based on the detected pathogens in these patients. Serum levels of Th1 cytokines including interferon γ (IFN-γ) and tumor necrosis factor-α (TNF-α), and Th2 cytokines including interleukin 4 (IL-4) and IL-10, soluble toll-like receptor 2 (sTLR2) and 4 (sTLR4), soluble tumor necrosis factor receptor 1 (sTNFR1) and sTNFR2 were determined using enzyme-linked immunosorbent assay (ELISA) kits.Results:Serum levels of IFN-γ ( H=10.030, P=0.007) and IL-4 ( H=10.246, P=0.006) were significantly up-regulated in RSV-A single infection group and RSV-A coinfection group, however, levels of TNF-α ( F=1.154, P=0.322) and IL-10 ( F=1.738, P=0.188) were not significantly up-regulated. Area under the curve (AUC) of IFN-γ and IL-4 were 0.866 (95% CI: 0.767-0.966, P=0.002) and 0.986 (95% CI: 0.956-1.000, P=0.001), respectively. The levels of sTLR2 ( H=1.165, P=0.559) and sTLR4 ( H=1.657, P=0.437) were not significantly changed. Levels of sTNFR1 ( H=11.431, P=0.003) and sTNFR2 ( F=3.411, P=0.041) were significantly up-regulated in RSV-A coinfection group and the concentration of sTNFR2 was higher than sTNFR1. Concentration of sTNFR1 was peaked in RSV-A coinfected with bacterium group and RSV-A coinfected with dual bacteria group. AUC of sTNFR2 was 0.967 (95% CI: 0.911-1.000, P=0.002). Conclusions:Expressions of immune-associated molecules, including IFN-γ, IL-4, sTNFR1 and sTNFR2 were significantly changed in patients infected with RSV-A, and these immune-associated molecules maybe engage in the interaction and pathogenesis between RSV-A and host.

Objective:To analyze the prevalence and clinical features of respiratory syncytial virus (RSV) in Chengde city.Methods:From August 2022 to June 2023, throat swabs and clinical data of 478 hospitalized children with respiratory tract infection in the Chengde Central Hospital were collected. Real-time quantitative PCR was used to detect the molecular epidemiology of RSV-A and RSV-B subtypes and analyze the clinical features of patients with RSV infection.Results:Among the hospitalized children, 67.57% (323/478) tested positive for RSV. The outbreak of RSV infection was caused by RSV-A subtype. The peaks of RSV-A infection occurred from November to December, 2022 and May to June, 2023. There were 86.07% (278/323) of the RSV-A-positive cases had mixed infection with other pathogens, primarily bacterial pathogens with Streptococcus pneumoniae being the most common, followed by Klebsiella pneumoniae. Influenza virus A was the most common viral pathogens causing mixed infection. The level of lactate dehydrogenase was higher in the patients with single RSV-A infection than in those with mixed infection ( Z=2.396, P=0.017), and higher than the normal upper limit. Compared with the single infection group, the mixed infection group had higher white blood cell count ( Z=2.417, P=0.016), neutrophil ratio ( Z=3.218, P=0.001), C-reactive protein level ( Z=1.998, P=0.046) and creatinine level ( Z=2.107, P=0.035), and lower lymphocyte ratio ( Z=3.205, P=0.001), but they were all within the normal range. There were no significant differences in the clinical features between RSV-A-positive patients co-infected with bacteria or other viruses (all P>0.05). Conclusions:RSV-A is the leading cause of respiratory tract infection in children in Chengde from 2022 to 2023, and often co-detected with bacteria. The mixed infection with other respiratory pathogens is related to the clinical features of patients with RSV-A infection.