Efforts are being made to build up platforms to support basic research of ophthalmology by organizing capable staff,adequate instruments,technical assistance,and by applying practical evaluation and cultivating healthy cultural environment.While significant progress has been achieved with scientific publications and international academic exchanges,the ophthalmology society still has a long way to go to enhance the basic research in the field of eye science.Utilization of modern biomedical tools,technologies and strategies will undoubtedly further improve the efficacy and production in the area of basic research of ophthalmology in this country.

Objective To prepare and identify monoclonal antibodies (mAb) against histidine-tag (His-tag ) .Methods Balb/c mice were immunized with polypeptides containing 15 histindine(His)-coupled BSA and this fusion was prepared according to con-ventional methods .Indirect ELISA was used to screen the positive clones and limited dilution for further cloning .After purified with Protein G affinity chromatography ,these antibodies for His-tag were detected for antibody titer ,relative affinity as well as subtypes by using ELISA .The specificity of these mAb was identified by ELISA and Western blotting analysis .Double-antibody sandwich ELISA was composed of these mAb paired with the corresponding recombinant protein of specific antibody ,which was used to de-tect the recombinant protein quantitatively .Results 5 hybridoma cell lines stably secreting anti-His-tag IgG1(κ) were screened .A-mong these antibodies ,1-G2 was of the highest affinity ,reaching 1 .27 × 1010 ,which could combine with different recombinant pro-teins containing His-tag in the experiment of ELISA and Western blotting .1-G2 also could be used to detect recombinant PCT pro-tein containing His-tag quantitatively by using double-antibody sandwich ELISA with antibody of PCT ,the sensitivity of detection reached 4 .6 ng/mL .Conclusion The mAb against for His-tag with high specificity ,affinity and secreted stably are successfully prepared .This prepared antibody could be used in ELISA and Western blotting to detect a variety of His-tag .

Objective To study the expression of PTEN,Fas and DCR3 in human gastric cancer and evaluate their effects on carcinogenesis,cell proliferation,invasion and metastasis of gastric carcinoma,as well as clinical significance.Methods The expression of PTEN,Fas and DCR3 were detected by immunohistochemical method (streptavidin-peroxidase) in the tissues of 75 primary gastric carcinomas and 15 gastric ulcers.Results The positive expression rate of DCR3 in gastric carcinoma tissues was significantly higher than that in normal tissues,and the expression of PTEN and Fas was obviously lower than that in normal tissues (P<0.01).The expression of DCR3 was negatively correlated with Fas and PTEN respectively (r=-0.720,P<0.001; r=-0.336,P<0.001).There was a positive correlation between PTEN and Fas.The expression of PTEN,Fas and DCR3 was closely correlated with lymph node metastasis,clinical stage,histological differentiation grade and tumor depth of gastric cancer,but not related to sex,age and tumor size (P>0.05).Conclusion PTEN,DCR3 and Fas have mutual restrictive relationships in the process of invasion and metastasis in gastric cancer.Detecting the expression of PTEN,Fas and DCR3 in gastric cancer is helpful to judge the malignant biological behavior of gastric cancer at a different angle,which may be considered as reliability index in clinic diagnosis,judging curative effect and monitoring the prognosis of gastric carcinoma.

Objective To understand the female sex workers and floating population in Yuzhong district of AIDS intervention effect ,To provide the basis for future planning of AIDS intervention in high-risk population .Methods 2011 in Yuzhong area ,By questionnaire survey and serological testing on 400 FSWs and 400 floating population .Results The rate of knowledge about AIDS among female sex workersis was 91 .75% ,higher than the floating population(73 .5% );female sex workers and floating population in a recent condom use rates was 74 .25% and 74 .71% ;the rate of condom insist to use was 48 .25% and 39 .08% ;the rate of Hiv prevalence was 0 .50% and 0 .75% .Conclusion To further increase the female sex workers and floating population coverage of in-terventions ,the role of a bridge cut off the high-risk groups in AIDS transmission of sexual contact ,and has important significance to the spread of AIDS control in Yuzhong District .

OBJECTIVE: To study the changes of serum vascular endothelial growth factor (VEGF) and beta 2-microglobulin levels before and after radiotherapy in 58 patients with nasopharyngeal carcinoma (NPC), and to elucidate the clinical significance of VEGF and beta 2-microglobulin test before radiotherapy. METHODS: Serum VEGF level was measured by sandwich ELISA in 58 patients with NPC and 24 healthy individuals, and the serum beta 2-microglobulin was assayed with time-resolved fluoroimmunoassay. RESULTS: The serum VEGF level in patients with NPC was (174.0+/-130.0) ng/L, higher than (134.1+/-66.6) ng/L of the healthy subjects, but the difference was not significant (P>0.05). The serum VEGF level was higher in T4 and IV a patients with NPC. No significant differences in the levels of serum VEGF were found among various N classifications in the patients with NPC. Patients with the serum VEGF level higher than 267.3 ng/L (mean+2 s of the serum VEGF level in the healthy individuals) had a shorter metastasis-free survival time (P<0.05). Although the patients with high beta 2-microglobulin level had a shorter survival time, the difference was not significant. CONCLUSION: The NPC patients with high serum VEGF level have a poor prognosis.

Objective To observe the effect of intravenous pre-injection of neostigmine on cardiovascular response(CVR) caused by neuromuscular electrical stimulation(NMES) in selective posterior rhizotomy(SPR) for patients with cerebral palsy.Methods 56 patients with cerebral palsy undergoing SPR at lumbarsacral segments under general anesthesia were randomly assigned to 2 groups: intravenous neostigmine 0.04 mg/kg(no more than 1 mg in total) and intravenous atropine 0.02 mg/kg(no more than 0.5 mg in total) 5 min before NMES in group N,and intravenous normal saline 0.12 ml/kg instead in group C.The systolic blood pressure(SBP) and heart rate(HR) at following time points: before skin incision(T1),before intravenous neostigmine/atropine or normal saline injection(T2),1 min after NMES(T3),and 10 min after NMES(T4).Results SBP and HR at T3 in group N were significantly lower than those in group C(P<0.01).No significant difference was found at T1,T2 and T4 between two groups(P>0.05).Cases who needed extra dose of fentanyl during NMES in group N were significantly lower than those in group C(P<0.01).Conclusion Intravenous pre-injection of neostigmine before NMES can effectively decrease the CVR caused by NMES.

Objective To observe the post-operative analgesic effect of flurbiprofen Axetil applied preoperatively on patients with spinal cord injury(SCI).Methods46 SCI patients undergoing spine surgery under general anesthesia were randomly divided into the flurbiprofen axetil group(group F)and control group(group C)with 23 cases in each group.Before cutting skin,flurbiprofen axetil(1 mg/kg)was injected intravenously in the group F,and normal saline(1 ml/kg)was infected intravenously in the group C.The scores of Visual Analogue Scale(VAS)for pain at postoperative 2,4,8,24,and 48 hours,and the incidence of side effects within 48 hours after operation were recorded.ResultsVAS scores at the time of 2,4,8,and 24 h after operation in the group F were significantly lower than that in the group C(P<0.05).But VAS scores of two groups at 48 h were not significantly different(P>0.05).The incidences of side effects of two groups were also not significantly different(P>0.05).ConclusionFlurbiprofen axetil applied preoperatively can release pain of SCI patients after spine surgery,and does not increase the incidence of side effects.

Objective To compare the characteristics of total intravenous anesthesia (TIA) with manual assisted ventilation and combined inhalation and intravenous anesthesia (CIIA) with mechanical ventilation in lower limbs orthopedics for patients with cerebral palsy.Methods 50 patients with cerebral palsy undergoing scheduled lower limbs orthopedics were randomly divided into the TIA group and CIIA group with 25 cases in each group. The patients of the TIA group were treated with manual assisted ventilation. Those of the CIIA group were treated with fast induction, endotracheal intubation, inhalated and intravenous anesthetics maintaining the anesthesia and mechanical ventilation. The recovery time after operation and post-operative side effects including nausea, vomiting, and psychomotor agitation of all patients were observed.Results The recovery time of the patients in the CIIA group was significantly longer than that in the TIA group ( P<0.01), and post-operative psychomotor agitation was significantly higher than that in the TIA group ( P<0.05). However, there was no significant difference in nausea or vomiting found between two groups ( P>0.05).Conclusion Patients treated with TIA is easier to recovery and has fewer side effects than those treated with CIIA.

Objective To investigate the carbohydrate metabolism during selective posterior rhizotomy (PCR) at lumbar and sacral regions on cerebral palsy children (CPC).Methods62 CPC who received SPR at lumbar and sacral regions under combined intravenous and inhalation anesthesia were tested with the level of glucose (Glu) in plasma at different time during the operation.Results and ConclusionThere is no significant difference of Glu at different time during the operation, however, the Glu level shows an elevating trend at 3 and 10 minutes after posterior root electric stimulation.

ObjectiveTo compare costs of combined intravenous and inhalation anesthesia (CIIA) and total intravenous anesthesia (TIA) when lower limbs orthopedics (LLO) performed on cerebral palsy children (CPC).MethodsTo analyze data of 49 CPC who received LLO under CIIA and TIA,and compare costs of two anesthetic styles.ResultsTIA had a significantly lower cost than CIIA (P<0.001),and showed a lower incidence of post-operation nausea and vomiting.ConclusionTIA can decrease cost of anesthesia and post-operation nursing compared with CIIA.