Objective To explore the cause of anastomotie fistula after esophagus cancer and cardiac cancer operation and its prevention and treatment.Methods After excising the tumors,the esophagogastrostomy was performed in 60 cases,in which the cervical anastomose in 48 cases and intrathoracic anastomose was adopted in 12 cases.We retrospectively analyzed its prevention and treatment. Results Caevial anastomose in 48 cases,4 patients with anastomotic fistula in the died,while none of 12 cases in the cervical anastomose died. Conclusions Careful procedure and improving the anastomotic methods are the key factors to reduse the anastomotic fistula,the accurate control of infection and full nutritive support are the effective methods to decrease the mortality rate of this disease.

Objective To observe postoperative analgesia effect of sacral canal injection of dezocine before procedure for prolapse and hemorrhoids operation.Methods A total of 160 patients with mixed hemorrhoid were divided into two groups:observation group(n=80)and control group(n=80).Dezocine 5 mg was injected in sacral canal before operation in observation group and same dose of saline was injected in control group.The postoperative analgesia effect,complication and general condition of patients were observed.Results Compared with the control group,significandy more slightless in pain were found in observation group.There was no effect on the wound healing time of patients.The rate of complication in observation group was lower.Conclusion The sacral canal injection of dezocine was a safe,simple and effective analgesic method.

Objective To investigate the diagnostic significance of lysophosphatidic acid (LPA) ,tumor specific growth factor (TSGF) and carbohydrate antigen 153 (CA153) in breast cancer .Methods Serum levels of LPA ,TSGF and CA153 were detected in 97 cases of breast cancer (cancer group) ,43 cases of benign breast disease (benign group) and 50 healthy females (control group) .Diagnostic significances of these parameters were analyzed .Results Serum levels of LPA ,TSGF and CA153 in cancer group were significantly higher than benign group and control group (P<0 .05) .Diagnostic sensitivity and specificity of combined detection of LPA ,TSGF and CA153 was higher than individual testing (P<0 .05) .Conclusion Combined detection of tumor mark‐ers could improve the diagnostic specificity and sensitivity of breast cancer ,and provide reliable experimental basis for diagnosis .

Objective To investigate the value of high sensitive cardiac troponin T(hs-cTnT),human heart fatty acid binding protein(H-FABP),B-type natriuretic peptide(BNP),ischemia modified albumin(IMA) and creatine kinase(CK-MB) in the diagnosis of myocardial injury in preeclampsia.Methods A total of 50 women of normal pregnancy in preganancy group,and 50 healthy women without pregnacy were collected in control group,167 women in preeclampsia group (42 cases with myocardial injury,125 cases without myocardial injury).The serum IMA were detected by albumin cobalt binding test,H-FABP were detected by ELISA,hs-cTnT and BNP were detected by microparticle chemiluminescence immunoassay and CK-MB were detected by immuno-suppression.Results Five cardiac markers levels in myocardial injury group of preeclampsia group were significantly higher than those in healthy control group and normal pregnancy women and non myocardial injury group[(t=8.521,7.489,7.256;7.561,6.897,6.235;12.314,9.236,10.251;13.657,11.023,12.031;11.301,10.364,15.567),(P=0.008,0.030,0.035;0.027,0.031,0.033;0.002,0.005,0.003;0.002,0.004,0.003;0.003,0.004,0.001)].There was no significant difference in hs-cTnT among control group and normal pregnancy women and non myocardial injury group[(t=1.678,1.401;1.887,1.784),(P=0.339,0.401;0.289,0.398)].IMA、H-FABP、CK-MB and BNP in normal pregnancy women and non myocardial injury group were higher than in control group [(t=4.784,4.021;3.894,3.784;5.801,5.215),(P=0.024,0.032;0.037,0.041;0.021,0.029)].There was no significant difference in IMA,H-FABP,CK-MB and BNP between normal pregnancy women and non myocardial injury group [(t=1.325,1.257,1.241);(P=0.451,0.329,0.378)].The sensitivity of combined detection of five cardiac markers in preeclampsia myocardial injury was significantly higher than that in single marker(χ2=3.021,3.561,4.215,4.496,5.249;P=0.027,0.024,0.019,0.015,0.009).Conclusion When myocardial damage in pregnant women was determined by cardiac markers,pregnancy should be taken into account as a special physiological cycle.The combined detection of five cardiac markers is conducive to early diagnosis of ischemic myocardial injury in preeclampsia.

During training cardiothoracic surgery advanced doctors,the teachers instituted reasonable feasible training plans for every advanced doctor individually according to their own characteristicses.At the same time,the teachers paid much attention to the basal knowledge and basal theories,the basal skills,which we called "three basic" training.In the training,the teachers also emphasized the clinic ideations training.Moralities and ethos working as doctors were brought forward,so that advanced doctors' legal consciousnesses and the ability of dealing with medical disputes.Advanced doctors were trained in their research and study abilities by using the advantages of teaching hospital,and the effects were satisfactory.

Objective To gain a higher quality of pathophysiology experiment teaching. Method To improve greatly such aspects as raising the capabilitys of teachers, arousing the interest of students, cultivating the studetns’ capability of design, solving questions and innovation. Results We have a conspicuous improvement at the quality and effect of pathophysiology experiment teaching.Conclusion To gain a higher quality of pathophysiology experiment teaching, we should make great improvement in raising the capabilities of teachers, arousing the interest of students, cultivating the studetns’ capability of design, solving questions and innovation.

Objective:To study biomedical engineers’ working function in medical device adverse events monitoring.Methods: According to the core function of biomedical engineering discipline and the related theory of adverse events, we analyzed the complexity and difficulty of medical device adverse event monitoring, pointed out the major jobs of biomedical engineers and combined with the medical device maintenance case analysis.Results: The risk management of medical device is the working focus of biomedical engineers. Biomedical engineers have professional advantages in medical device adverse event monitoring.Conclusions: Based on discipline advantages and working core functions, the biomedical engineers can report high quality medical device adverse events and analyze and deal with them in time.

Objective To discuss the application of improved agarose electrophoresis for detecting bone alkaline phosphatase(BALP) isoenzymes in the diagnosis of metastasis in prostate carcinoma(PCa).Methods Serum ALP isoenzymes in 50 persons undergoing physical examination and 163 cases of PCa were detected by the improved agarose electrophoresis after treating serum by neuraminidase.Results (1)The resolution of liver and bone ALP by the common agarose gel electrophoresis was lower,and which was increased by the modified agarose electrophoresis.(2)Total ALP and BALP in the PCa group were higher than those in the control group;total ALP and BALP activity in the PCa bone metastasis group were higher than those in the control group and non-metastasis group(P<0.05),there were no statistical difference in BALP and total ALP between the non-bone metastasis group and control group(P>0.05).BALP and total ALP in bone metastasis of prostate carcinoma were higher than no bone metastasis.Conclusion Bone ALP has a certain correlation with PCa bone metastasis,also between bone ALP and bone metastasis of carcinoma.After treating serum by neuraminidase,adopting the improved agarose gel electrophoresis for separating ALP isozymes contributes to the clinical auxiliary diagnosis of PCa bone metastasis.

Objective:To explore the diagnosis and treatment of thymoma with pure red cell aplasia(PRCA).Methods: From May 1999 to March 2006,the cases of thymoma with PRCA were treated.The clinical data was analysed and the Chinese literature was reviewed.Results:The cases of thymoma with PRCA accounted for 4.2%(3/70)of total thymoma treated in the same period,2 cases underwent thymomectomy.No recurrences of thymoma or PRCA was found in 1 case 3 years and 1 case 6 months after operation.There was no effect on 1 case undergoing surgical exploration only.Conclusion: Thymoectomy is the first choice for thymoma with PRCA.The long term effects should be further followed up.

Objective:To Summarize the experience of atrail septal defecf (ASD) closure with occluder by minimally invasive operation. Methods:Six patients with ASD were treated by microcnvasive method to close ASD with special occluder device. The age of patients was 27 to 57 years. The diameter of ASD was 22 to 40mm. All patients were anaesthetized and a 2-3cm long incision was made in the 4th intercostal space of right side of sternum. All the cases were treated with special occluder device under the echocardiography guidance. Results:ASDs of 5 cases were successfully occluded. No Complications happened. They were discharged from the hospital 3 to 5 days after the operation. After 3 to 15 months tracing,no risidual shunt was found,heart size and function were improved. Conelusion:The minimally invasive operation to close ASD with special occluder device is safe and efficient. The Long-term follow-up remains to be studied. [