Objective To evaluate the cerebrovascular reserve(CVR) in patients of ischemic stroke with cerebrovascular stenosis by Xenon-enhanced CT. Methods Twenty subjects of ischemic stroke with cerebrovascular stenosis were recruited. All subjects were examined by Xenon-enhanced CT before and after acetazolamide (ACZ) challenge test to quantitatively measure the regional cerebral blood flow (rCBF) and CVR. Results We compared the rCBF in the corresponding region supplied with stenosed artery (divided manually) with that in the region of normal side. There was no significant difference in resting rCBF but in CVR [ipsilateral side vs. normal side ( 5.9 ± 24. 3 ) % vs ( 25.9 ± 32. 6 ) % , P ＜ 0. 05]. Conclusion Impaired CVR is an important character of the patients with cerebrovascular stenosis suffered from ischemic stroke.

Methionine dependence is one of characteristic metabolism deficiency in cancer cells.Recently a series of researches focusing on key enzymes of methionine metabolism demonstrated that several molecular events might relate with cancer cell methionine dependence(i.e.,methionine synthasesingle nucleotide polymorphism,methythioadenosine phophorylasegene deletion,down regulation of thymidylate synthase translation;up regulation of methylenetetrahydrofolate reductase expression).Cell apoptosis induced by intracellular signal transduction dysfunction might be another potential mechanism.

objective: To develop an accurate, reliable and efficient carotid artery compression training device .Methods:Adopting present technology for production of skin dilators in combination with anatomical characters of human carotid artery, an implantable carotid artery training occluder (ICATO) was designed and manufactured. The mechanical properties were tested and the carotid artery occlude effect was investigated in 12 dogs. Results: The expansion stress of the water cyst and duct of the occluder was 40 kPa, that of the water imput valve 30 kPa,tear stress of the nylon cloth was 5 kg. The carotid artery could be completely blocked at the pressure of 19 kPa produced by the occluder. Conclusion: ICATO can obstruct carotid artery for the purpose of compression training of carotid artery.

Congenital hydronephrosis is a common disease in children,causes and pathogenesis re-mains unclear.Hydronephrosis formation process is a slow and gradual development to the dynamic process of renal fibrosis.It involves a variety of cells,cytokines and ECM,more than aspects of interaction and mu-tual adjustment.The study found abroad that CTGF is closely related to the formation of congenital hydrone-phrosis.This article reviews the recent progress made CTGF relationship with congenital hydronephrosis formed on.

The peptidyl prolyl cis/trans isomerase Pin1 specifically binds phosphorylated Ser/Thr-Pro protein motifs and catalyzes the cis/trans isomerization of the peptide bond,changes the conformation and influences the stability and activity of the substrates.To date,a subset of proteins has been identified as substrates for Pin1.Pin1 interacts with its substrates and plays crucial roles in cell cycle,neural pathology and immune response.Accumulating studies have revealed that Pin1 isomerase activity is regulated by its post-translational modifications,including phosphorylation and oxidation.Over-expression or regulative imbalance of Pin1 plays an important role in the pathogenesis and therapeutics of human diseases such as cancer and AD.

With the progress of human civilization,the harmonious development of society is gradually realized by different researchers.Medicine,as a science directly serving humans,emphasizes more on humanism than other sciences.Medical research is not only characterized by scientificity and innovativeness,but also by ethicality.More and more biomedical journals are paying attention to issues of ethics.This review discussed the ethical issues in academic medical writing based on the Uniform Requirements for Manuscripts Submitted to Biomedical Journals published by International Committee of Medical Journal Editors (ICMJE) and the discussions by Committee on Publication Ethics (COPE).

Objective To investigate the correlation of killer immunoglobulin-like receptors(KIR)gene polymorphism with bone marrow failure syndromes(BMFS). Methods SSP-PCR was used to examine the genotypic makeup of KIR in patients with aplastic anemia( AA), myelodysplatic syndrome (MDS) and healthy controls in our department. Results All the 16 KIR genes which had been prescribed were identified. The frequencies of KIR-2DS1, 2DS2, 2DS3, 2DS5 and 3DS1 genes were showed increased in patients with AA, MDS than in healthy controls. The patients with AA had lower frequency of KIR-2DS5 than the patients with MDS. Conclusion The increased frequencies of these activated KiRs in patients with MDS and AA suggest that the abnormal immunogenetic might be related to the pathogenesis of BMFS.

objective: To develop injectable tissue engineered bone through injection of osteoblasts/alginate composite in rabbits. Methods: Bone marrow cells isolated from iliac bone of New Zealand rabbits were cultured and induced to differentiate into osteoblasts.The osteoblasts were mixed with 25 g/L sodium alginate solution to generate osteoblasts/alginate composite with final cellular density of 5?10 6/ml. 0.17 g of sterilized CaSO 4 powder was then added to 2 ml osteoblasts/alginate. The mixture was injected into the dorsal subcutaneous tissue at left side of 6 New Zealand rabbits. The alginate composite without osteoblasts was injected into the right side as the control. 4 and 8 weeks after implantation, the bone formation was evaluated by means of gross, X ray and histological observation. Results: 4 weeks after implantation, cartilage formation was observed and 8 weeks after implantation,new mature bone was observed in the osteoblasts/alginate composites. No new bone formation was observed in all of the control specimens. Conclusion: Calcium alginate can be used as a carrier in injectable bone tissue engineering, and new bone can be created through injection of osteoblasts/alginate composites in immune animals.

Objective To investigate the effect of low intensity microwave electromagnetic fields (EMF) on energy metabolism of cerebral cortical neurons of postnatal rats. Methods The cultured neurons were exposed to low intensity 900 MHz continuous microwave EMF (SAR=0.38-3.22 mW/g) 2 hours a day for 4 to 6 consecutive days. The activity of cytochrome oxidase(CCO) was taken as the index for energy metabolism level. Results The results of cytochemistry showed that compared to the sham-exposed, a significant decrease of cytochrome oxidase activity in exposed neurons(0.38-3.22 mW/g P

Objective:To investigate the promoting effects of interleukin-13(IL-13)on the proliferation of airway smooth muscle cells(ASMC)in mice and its mechanism.Methods:Murine ASMC were isolated and subcultured.IL-13,IL-13plus AG1478,IL-13plus neutralizing antibody to TGF-?,IL-13plus neutralizing antibody to EGF,IL-13plus neutralizing antibody to HB-EGF,IL-13plus dexamethasone were added to the media,respectively.MTT and 3 H-TdR incorporation assays were used.Apical media were analyzed for the presence of soluble TGF-?using ELISA.Results:The D 595 value of MTT assay and the cpm value of 3 H-TdR incorporation assay of IL-13group were significantly higher than those of control group(P