Objective To investigate the effects of mesalazine combined with Xianglian pills on cytokines and immunocytes in ulcerative colitis. Methods A total of 86 patients with ulcerative colitis from our hospital were collected and randomly divided into experimental group and control group with 43 cases in each group.Patients in the control group were treated by mesalazine, patients in the experimental group were treated by mesalazine combined with Xianglian pills treatment.The cytokines and immunocytes levels and efficacy between two groups were compared.Results After treatment, the clinical effective rate of the control group 69.77% was lower than the experimental group 88.37%, with statistical significance ( P <0.05 );compared with the control group, the TNF-αlevel of the experimental group was lower than the control group, IL-2, IL-4 and IL-10 levels were higher than the control group;the serum TFF1 and TFF3 levels were higher than the control group;CD3 +, CD4 +/CD8 +and NK cells levels were higher than the control group, the level of CD8 +was lower than the control group, with statistical significance ( P<0.05 ) .Conclusion Mesalazine combined with Xianglian pills in the treatment of patients with ulcerative colitis could reduce the inflammatory reactions and improve the clinical efficacy, with lower adverse reactions.

The importance of course arrangement and standardization construction for the course of hyperbaric oxygenation medicine was expounded in this review.In addition,the measures and experiences on textbook choosing,teaching content assignment,teaching methods designing,assessment means innovation and teaching personnel training were also discussed.The teaching system of the course of hyperbaric oxygenation medicine can be improved and references for thc dcvelopment of clinical hyperbaric oxygenation medicine courses can be provided through these measures.

Objective To investigate the clinical characteristics of clavicular defect and nonunion after operation and evaluate the clinical effects of vascularized pedicled pectoralis major clavicular periosteal flap and locking plate fixation plus bone morphogenetic protein (BMP) implantation. Methods From January 2004 to April 2008, 12 patients (mean age, 42.8 years old) with clavicular defect and nonunion were treated in Zhongshan Hospital. The internal fixators were removed, and then fibrous scars and sclerotic bones were cleared. The clavicular detects were fixed with locking plate, with implantation of the BMP. The wound was covered with vascularized pedicled pectoralis major clavicular periosteal flap. Postoperative functional rehabilitation exercises were performed. Results All patients were followed up for 8-24 months (mean 1.2 years). The clinical results were evaluated by Constant-Murley scoring system. The clavicular defects were healed in all patients within 4-7 months, and the internal fixators were successfully removed from some of the patients. Conclusions Vascularized pedicled pectoralis major clavicular periosteal flap combined with locking plate fixation plus BMP implantation is effective in the treatment of clavicular defect and nonunion.

Objective To analyze the clinical effects, as well as its advantages and disadvantages, of laparoscopic surgery for appendicitis in obese patients. Methods Clinical data of 80 obese patients receiving appendectomy, including 38 cases of open appendectomy and 42 cases of laparoscopic appendectomy, were analyzed retrospectively.The operative time, blood loss, analgesic and antibiotic use, postoperative fever, postoperative complications, length of hospital stay, and hospitalization cost were compared between the two operative methods. Results Conversions to open surgery were required in 2 patients in the laparoscopic group. There was no significant difference in operative time between the 2 groups (P >0.05).Compared with the open group, the laparoscopic group had less blood loss [(14.98 ±12.77) ml vs.(31.58 ±19.00) ml, t=-4.550, P=0.000], shorter time of postoperative antibiotic use [(2.7 ±1.0) d vs.(4.1 ±1.2) d, t=-5.470, P=0.000], less postoperative analgesics needed [5.0%(2/40) vs.26.3%(10/38),χ2 =6.802, P=0.009], less drainage [2.5% (1/40) vs.18.4% (7/38), χ2 =5.367, P=0.021], less postoperative fever [5.0%(2/40) vs.23.7%(9/38),χ2 =5.616, P=0.018], less postoperative wound healing [5.0%(2/40) vs.21.1%(8/38),χ2 =4.493, P=0.034], and shorter hospital stay [(5.9 ±3.2) d vs.(8.7 ±4.1) d, t=-3.345, P=0.001], but the higher cost of hospitalization [(7800 ±396) yuan vs.(4914 ±434) yuan, t=30.716, P=0.000]. Conclusion For obese patients with appendicitis, laparoscopic appendectomy has less surgical trauma, faster recovery, less postoperative pain, fewer complications,and shorter hospital stay, being a preferred method of treatment.

Heart ; physiopathology ; Humans ; Infant, Newborn ; Long QT Syndrome ; complications ; Male ; Tachycardia, Ventricular ; complications ; Torsades de Pointes ; complications

Bacillus subtilis is a good producer of mannanase.This is the first study to investigate mannanase production under solid state fermentation(SSF) by Bacillus subtilis.Bacillus subtilis WY34 was used for the production of extracellular mannanase in the SSF of wheat bran.The highest mannanase activity was 7,650 U/g dry matter when the following conditions were used: wheat bran 5g,initial moisture content 71%,Tween-80 1%,konjac powder 0.4 g,initial pH 7.0,incubation temperature 50 ℃.Enzyme production was increased 178% after optimization.

Objective: To study the pathological characteristics of columnar alteration with prominent snouts and secretions (CAPSS) and its relationship with incidence of breast carcinoma. Methods: One hundred forty-four CAPSS specimens identified by breast core needle biopsies(CNB) were retrospectively evaluated. The CAPSS specimens were subdivided into lesions with (n=108) and without (n=36) atypical ductal hyperplasia and their pathological findings were compared. Results: In situ and invasive carcinomas were found in 21.5% (31/144) of the specimens, including those with and without atypical hyperplasia. We also found that specimens with atypical hyperplasia had a higher malignant incidence than those without atypical hyperplasia (P=0.001). Conclusion: CAPSS, especially when with atypical ductal hyperplasia, are associated with breast carcinoma.

Objective To study the biomechanics of simple locking proximal femoral plate (LPFP),LPFP combined with calcium phosphate cement (CPC) and proximal femoral nail antirotation (PFNA) in treatment of unstable intertrochanteric fractures.Methods Thirty-six femurs from 18 specimens with no pathological defects,fractures,malformations or neoplasms were enrolled in the study and were divided into three groups (Groups A,B and C),with 12 femurs per group.Intertrochanteric fractures were duplicated in accordance with Evans classification of type Ⅲ A.The fractures in Group A were treated by LPFP combined with CPC fixation,that in Group B treated with only LPFP fixation,while that in Group C fixed with PFNA.A comparative mechanical test of femoral specimens was performed in a special clamp.Results Axial compression test showed linear change of the load-displacement of three surgical fixations for intertrochanteric fractures under biological load.The measurement of torque-torsion angle of three fixations for intertrochateric fractures showed that the torque was enlarged with increase of torsion angle till the failure of internal fixation.Conclusion LPFP combined with CPC or PFNA can attain solid fixation of unstable intertrochanteric fractures.

AIM: To investigate the effects of globular adiponectin (gAd) on the expression of adiponectin receptor 1 (AdipoR1) in human umbilical vein endothelial cells (HUVECs), and on the apoptosis induced by advanced glycation end products (AGEs). METHODS: HUVECs were treated with the indicated concentrations of AGEs for 24 h or 48 h in vitro. The cells in gAd treatment group were pretreated with gAd for 24 h, and then were treated with AGEs for another 24 h or 48 h. Cell variability was quantified by MTT assay. Apoptotic cells were detected by flow cytometry with Annexin-FITC/PI double staining. AdipoR1 mRNA in the cells was determined by quantitative real time reverse transcriptase-polymerase chain reaction (RT-PCR). RESULTS: The viability of HUVECs treated with AGEs decreased significantly as compared to the cells treated with HSA (control, P<0.05). Under the same condition of AGEs exposure, the viability of the cells treated with gAd was greatly higher than that of the cells without gAd treatment (P<0.01). The apoptotic rate of HUVECs was significantly elevated by AGEs treatment vs HSA treatment observed by Annexin V-FITC/PI double staining analysis with flow cytometry (P<0.05). Under the condition of AGEs stimulation, the apoptosis of HUVECs was decreased by pretreatment with gAd as compared to that of the cells without gAd treatment (P<0.01). Measured by quantitative real time PCR, AGEs decreased the expression level of AdipoR1 mRNA and gAd increased the expression of AdipoR1 mRNA contrarily (P<0.05). CONCLUSION: AGEs increase the apoptotic rate of HUVECs in a concentration dependent manner and gAd promotes the AdipoR1 mRNA expression.

Objective To analyze the clinical features of infective endocarditis with positive antineutrophil cytoplasmic antibodies ,and compare with ANCA associated small vessel vasculitis(AASV). Methods Three IE patients with positive ANCA were analyzed, and 13 cases from literatures were reviewed. Results Sixteen patients had positive anti-PR3 ANCA, in which 2 cases had both positive (anti-PR3 and anti-MPO ANCA) ANCA. All patients had some clinical manifestations mimic AASV, including fever ( 13/16, 81% ), rash (8/16, 50% ), rapidly progressive glomerulonephritis (7/16, 44% ), splenomegaly (6/16, 38% ). Streptococcal species were identified in 12 patients, and cardiac valvular abnormalities were demonstrated in all patients. All patients except 2, who died of cerebral hemorrhage followed by cerebral infarction, recovered with antibiotic therapy. Conclusion Infective endocarditis sometimes can have the same clinical features as AASV, so physicians should carefully differentiate between them when dealing with patients with positive ANCA antibodies.