Tumor microenvironment is the key factor that influences the invasion and metastasis of tumor cells.Exosome is the particle by which cells can make communication in microenvironment.MicroRNA (miRNA)is widely involved in physiological processes,and plays an important role in the stability of tumor environment.MiRNAs in exosome play roles in tumor cell proliferation,extracellular matrix remodeling,and then promote the metastasis,invasion and angiogenesis of tumor.Thus,the expression of oncogenes can be blocked by regulating the exosome production and secretion.It may shed light on the development of a thera-peutic strategy for tumor treatment.

MicroRNAs (miRNAs)are closely associated with the development,invasion,metastasis and prognosis of cholangiocarcinoma (CCA).The abnormal expressions of miRNA play important roles in regulating the genetic variation,cell cycle,invasion and metastasisability and apoptosis of CCA.MiRNAs are hopeful for being used as the biological markers of early diagnosis,prognosis and treatment target.

Adenocarcinoma, Mucinous ; metabolism ; pathology ; surgery ; Aged ; Biomarkers, Tumor ; metabolism ; Carcinoembryonic Antigen ; metabolism ; Carcinoma, Pancreatic Ductal ; metabolism ; pathology ; surgery ; Carcinoma, Papillary ; metabolism ; pathology ; surgery ; Cystadenocarcinoma, Mucinous ; metabolism ; pathology ; surgery ; Diagnosis, Differential ; Duodenal Neoplasms ; metabolism ; pathology ; surgery ; Female ; Follow-Up Studies ; Humans ; Immunohistochemistry ; Male ; Middle Aged ; Mucin-2 ; Mucins ; metabolism ; Neoplasm Invasiveness ; Pancreatic Neoplasms ; metabolism ; pathology ; surgery ; Pancreaticoduodenectomy

To study the status of homozygous deletions of MTS1/p16 gene in squamous cell carcinoma of the larynx (LSCC) and to discuss the relations of homozygous deletions to the pathological grade and clinical stage of the tumor,Fifty-nine primary LSCC were examined for homozygous deletions of the MTS1/p16 gene by comparative PCR.Three specimens were eliminated for being incapable of comparison with others.Homozygous deletions of the MTS1/p16 gene were found in 9 of 56 tumors (16.07%).The rates of homozygous deletions of MTS1/p16 gene in well,medially,poorly differentiated tumors were 17.24%(5/29),11.76%(2/17),and 20.00%(2/10) respectively.There had no significant difference in various pathological grades of tumors.Homozygous deletions rates of tumors in stagesⅠ～Ⅱand in stagesⅢ～Ⅳwere 5.41% (2/37) and 36.84%(7/19) respectively.The rate of homozygous deletions in stages Ⅲ～Ⅳtumors was significantly higher than that in stagesⅠ～Ⅱ(P＜0.01).Homozygous deletions of the MTS1/p16 gene were correlated well with clinical stages.Our data suggested that homozygous deletions of MTS1/p16 gene might be one of the genetic events in the development of the tumor and might play a role in malignant progression of LSCC.

Adult Clonorchis sinensis not only occurs in human hepatic duct,but also in the liver,gallbladder and bile ducts of animals,including dogs and cats,thus causing clonorchiasis—one of important parasitic zoonoses. In present study,we dis?sected a domestic cat in which a total of 736 pieces of trematodes,identified as Clonorchis sinensis,were detected in the liver and cholecyst. The findings indicate that Clonorchis sinensis may be endemic in domestic animals in Wuhu area,and observe our awareness in prevention of the parasites in house pets.

Objective To explore CT diagnostic values of cardiac carcinoma.Methods CT findings of cardiac cancer comfired pathologically in 50 cases were analysed.All case were examined with spiral CT.Results The thickness of gastric cardia wall was found in all 50 cases,soft tissue masses was found in 25 cases,adjacent tissue invasion was found in 18 cases and metastasis of lymph nodes was found in 33 cases.Conclusion CT is a valuable method in demonstrating the size of cardiac carcinoma,its adjacent tissue infiltration and lymph node metastasis.It plays important role in the planning of treatment in clinical practice.

Child A.The concentration of CGRP varied positively with the diameter of portal vein(r=0.60,P

OBJECTIVEBy observing the adipogenic and angiogenic microenvironment impact on the morphology of newly generated tissue for exploring the key factors which inducing mature adipose tissue regeneration in tissue engineering model.

METHODS24 healthy 6 months' New Zealand rabbits were picked and put into four groups according to different microenvironment. Every group has 6 rabbits and divided as follows: no axial-blood supply fat flap(0 ml), granular fat only(0.6 ml), axial blood vessel only (0.05 ml), axial vascularized fat flap ((0.6 ml). We separated or combined adipogenic and angiogenic environment within these groups. After 8 weeks, samples were harvested for histologic observation including macroscopic observation, volume analysis and HE testing.

RESULTSIn granular fat group, its volume decreased by (0.25 ± 0.10) ml after 8 weeks as the shortage of blood supply and finally it could be enveloped. In axial blood vessel group, its volume increased by (0. 37 ± 0. 04) ml after 8 weeks with fibrous tissue formation as shortage of adipogenic microenvironment. The no axial-blood supply fat flap group grew into(0.12 ± 0.03) ml, which can' t support large volume adipose tissue formation because of lacking independent blood supply. Only axial vascularized fat flap model could generate mature adipose tissue in large volume(3.45 ± 0.48) ml. The number of new capillary in every group was different after 8 weeks. By counting the numbers in every single view, no axial-blood supply fat flap group 15 ± 3.5)and granular fat only group(5 ± 2.5)had a significant difference with axial vascularized fat flap group 22 ± 5) respectively.

CONCLUSIONOnly both adipogenic or angiogenic microenvironment exist could induce mature adipose tissue in large volume in tissue engineering chamber model.

Adipogenesis ; physiology ; Adipose Tissue ; physiology ; Animals ; Neovascularization, Physiologic ; Rabbits ; Regeneration ; physiology ; Surgical Flaps ; blood supply ; transplantation ; Tissue Engineering ; methods

Data Mining ; Humans ; Medicine, Chinese Traditional ; Stomach Diseases ; therapy

OBJECTIVE: A retrospective analysis of the clinical efficacy on the surgery of maxillary sinus diseases via the endoscopic lateral nasal wall incision, and a discussion on the clinical application of this approach. METHOD: Eighteen cases of the maxillary sinus diseases diagnosed on the basis of the preoperative nasal endoscopy, CT scan or MRI, and pathologic finding. Among 13 patients underwent routine lateral nasal wall incision approach, including 4 of maxillary sinus hemorrhagic and necrotic polyps, 4 of maxillary sinus cyst, and 3 of the maxillary sinus fungal infection. Five patients underwent lateral nasal wall resection approach and thorough maxillary sinus lesions resection by nasal endoscope, including 3 of inverted maxillary sinus papilloma, a nasal sinus bone giant cell tumor and a spindle cell tumor. Patients were followed up for more than half a year, and the postoperative efficacy were observed. RESULT: The surgical cavity of the lateral nasal wall incision approach have luminal epithelium, well shapes of inferior turbinate, no recurrence of the lesion, and the lateral nasal wall resection patients with well luminal epithelium, without recurrence. All patients had no complications such as numbness, tears, etc. CONCLUSION Endoscopic incision of lateral nasal wall keep the nasolacrimal duct and inferior turbinate, help remove the entire sinus cavity lesion and retain the physiological function of the nasal cavity. Resection of the lateral nasal wall can reveal an ideal vision approach, which perform certain clinical value for the treatment of the inverted maxillary sinus papilloma and sinus cancer.
Cysts ; surgery ; Dissection ; methods ; Endoscopy ; Humans ; Magnetic Resonance Imaging ; Maxillary Sinus ; surgery ; Maxillary Sinus Neoplasms ; surgery ; Maxillary Sinusitis ; microbiology ; surgery ; Nasal Cavity ; surgery ; Papilloma, Inverted ; surgery ; Retrospective Studies ; Tomography, X-Ray Computed