Objective To investigate the expression of p38 mitogen-activated protein kinase(p38MAPK) in posterior horn of L5-S2 spinal cord in analgesia for chronic prostate pain with intrathecal injection of SB203580(p38MAPK inhibitor activity) in a murine model.Methods Forty-five male SPF SD rats were randomly divided into control group(n=5),prostatitis group(n=10) and SB203580 analgesia group(n=30,SB203580 was intrathecally injected).In the SB203580 analgesia group,complete Freund's adjuvant was injected into the prostate and SB203580 of 0.5?g/10?l,2.5?g/10?l and 12.5?g/10?l was intrathecally injected respectively.Five days and ten days after drug administration or simple modeling,phosphorylated p38MAPK(p-p38MAPK),glial fibrillary acidic protein(GFAP),tumor necrosis factor-?(TNF-?),nitric oxide(NO) level and induced nitric oxide synthase(iNOS) activity of L5-S2 spinal dorsal horn of rats were determined by Western blotting and ELISA assay,respectively.Results In the prostatitis group,the p-p38MAPK,GFAP,TNF-?,NO level and iNOS activity of L5-S2 spinal dorsal horn of rats at the 5th day and the 10th day were significantly higher than those of the control group and increased with time.The p-p38MAPK,GFAP,TNF-?,NO levels and iNOS activity at the 5th day and 10th day in SB203580 analgesia group were lower than those in the prostatitis group.Conclusion P38MAPK signaling pathway was involved in the mechanism of pain conduction and analgesia by SB203580 after prostatitis.The level of inflammatory factors in spinal cord can be effectively reduced by blocking the p38MAPK signaling pathway.

Objective To confirm the feasibility and security of the liver hanging maneuver in difficult right hemihepatectomy.Methods Medical records of 40 conseeutive patients considered for difficult right hemihepatectomy were reviewed.The patients were grouped into two groups in pairs:group 1 (using the hanging maneuver) in 20 and group 2 (using the traditional maneuver) in the left 20.Results It was successful in 20 patients and the overall feasibility was 100%.Operative duration,bleeding,transfusion,average stay and complications in group 1 were lower than those of group2.Conclusion The liver hanging maneuver not only has high feasibility but also improves the security in difficult right hemihepatectomy.

Objeetive To explore a new therapeutic way of transurethral drugs perfusion combined with ultrashort wave on treating chronic bacteria prostatitis.Methods 241 cases with chronic bacteria prostatltis were randomly divided into two groups.the treatment group were treated with transurethral perfusion combined with ultrashort wave.the control group were treated with transurcthral drugs perfusion.Results Total effective rate in the treatment group was 85.9%.that of the control group was 36.7%.It had a significant difference between two groups(P＜0.01).Conclusion Transurethral drugs perfusion combined with ultrashort wave could promote the curative effect of antibiotic maximumly,so it is an effective method to treat chronic bacterial prostatitis.

OBJECTIVE:To study the antihyperglycemic activity of Herba Taraxaci polysaccharides.METHODS:1,1-diphenyl-2-picryl-2-picrylhydrazyl(DPPH) radical scavenging and FRAP assays were used to analyze the antioxidant activity of Herba Taraxaci polysaccharides;the inhibitory activity of ?-glucosidase in vitro was tested by micromethod; the diabetic mice model was established using alloxan. After intragastric administration of Herba Taraxaci polysaccharide for 14 days,the fasting serum glucose and antioxidant parameters (SOD,MDA and GSH-Px) in dissected liver tissue were assayed. RESULTS:The TEAC values assayed by DPPH and FRAP methods were 564.98 ?molTE?g-1 and 1 399.55 ?molTE?g-1,respectively; and the IC50 value of Herba Taraxaci polysaccharides against ?-glucosidase was 49.27 ?g?mL-1.Herba Taraxaci polysaccharides had no effect on serum polysaccharides and antioxidant parameters in normal mice,but which significantly down-regulated the fasting serum glucose of diabetic mice especially at a high dosage(P

Objective To investigate the relationship between p38MAPK and astrocytes activation by detecting the changes of p38MAPK,TNF-?,NO and NOS in the substance P-induced activation of spinal cord astrocytes in vitro.Methods Astrocytes,cultured from spinal cord of SPF rat and identified by immunofluorescence method with GFAP antibody,were grouped into the control group,the SP stimulus group(SP group) and the SB203580 interrupt p38MAPK group(interrupt group) in which SP(10-7 mol/L) and SB203580(10 ?mol/L) were added to the supernatant for 12 h.The WB method,RT-PCR and ELISA were used to determine the changes of p38MAPK,phosphorylated p38MAPK,GFAP mRNA,TNF-?,NO and NOS in the astrocytes or supernatant at 1,3,12,24 and 36 h respectively.Results The GFAP positive rate of the cultured cells was higher than 95%.p38MAPK level in the SP group did not have any change,whereas p-p38 level increased significantly after 1 h and GFAP mRNA,NO,NOS and TNF-? level increased after 3 h.When p38MAPK pathway was inhibited by SB203580 in the SP+SB group,GFAP mRNA,NO,NOS and TNF-? were significantly reduced compared with those in the SP group.The level of p38MAPK,p-p38,GFAP mRNA,NO,NOS and TNF-? in the SB group did not have changes compared with those in the normal group.Conclusion These results indicate that p38MAPK signal pathway contributes to substance P induced activation of spinal cord astrocytes according to inflammatory factors attenuation after p38MAPK signal pathway interruption by SB203580.

Objective To evaluate the the safety and efficiency of precise liver resection for patients with primary liver cancer. Methods 86 patients with primary liver cancer were randomized to receive conventional routine hepatectomy (n=44) or the precise liver resection (n=42). Outcomes were compared between the precise hepatectomy group and the routine group, including, the blood loss, operation time, morbidity and mortality. Results There were significant differences in morbidity rates (7.1% vs. 20.5%; P<0.001), the blood loss [(320±315) ml vs. (613±526) ml;P<0.001) , postoperative alanine aminotransferase (ALT) value (in postoperation 7 d, 82.7 U/L vs.321.7 U/L; P<0.001) and length of hospital stay (12.3 d vs. 18.6 d; P<0.001) between precise hepatectomy and routine groups. The 1 year tumor recurrence rate and 1 year survival rate were 26.2%(11/42) and 78.6% (33/42) in precise liver resection group, 38.6% (17/44) and 65.9%(29/44) in routine liver resection group,with significant difference (P=0.010;P=0.018). Conclusion Precise liver resection is safe and effective in the treatment of liver tumor without much injury to patients.

BACKGROUND: Adipose tissue-derived stem cells receive a high attention in tissue engineering research. Adipose tissue-derived stem cells lack of specific surface marker, there is no effective purified method. Purified adipose is a simple method to elevate purify of stem cells. OBJECTIVE:To analyze how to purify adipose tissues before primary culture of adipose tissue-derived stem cells. DESIGN, TIME AND SETTING: The controlled animal experiment was performed at the Shanghai Animal Center of Experimental Medicine of Shanghai Sixth People’s Hospital between December 2007 and March 2008. MATERIALS: Four-week old Sprague Dawley rats were used for obtaining adipose tissues from the inguinal groove. METHODS: Adipose tissues from rat inguinal groove were dissected to educe superficial blood vessel and blood vessel branches. Both blood vessel inside and elliptic nodal tissues surrounding blood vessels were excised. MAIN OUTCOME MEASURES: Stained elliptic nodal tissues stained by Hematoxylin-Eosin were observed with a microscope to make sure what kind of tissues they are. The purified adipose tissues and unpurified adipose tissues were stained by Hematoxylin-Eosin. The differences in their tissue construction were observed using the microscope. RESULTS: Elliptic nodal tissues stained by Hematoxylin-Eosin were proved to be lymphatic tissues. The tissue construction of purified adipose tissues was pure, and the cellular component was simple. Conversely, the tissue construction of unpurified adipose tissues was complicated, and cells were various with complicated components. CONCLUSION: The component of adipose tissues used to primary cultured adipose tissue-derived stem cells is complicated. As resection of superficial blood vessel, skin and muscle tissues, blood vessel inside tissues and lymphatic tissues should also be excised.

Objective To investigate the application of “top-down” method in assessment of measurement uncertainty of bio-chemical detection indicators .Methods The assessment data of internal quality control and external quality and “top-down”method were used to assess the precision and accuracy of 26 biochemical indicators ,and the two variables above were combined to calculate the measurement uncertainty .Results Uncertainty of 18 biochemical indicators was compatible with the target uncertainty ,ac-counting for approximately 69 .2% of all assessment projects .Five indicators ,such as TBIL ,albumin ,CK ,calcium and magnesium , was not compatible with the target uncertainty ,accounting for approximately 19 .2% of all assessment projects .Conclusion The“top-down” method is effective and feasible for assessment of measurement uncertainty of biochemical detection indicators .

Objective: To compare the fixation strength of locking and non-locking anterior cervical plating initially and after fatigue.Methods:AO Cervical Spine Locking Plate(CSLP),Danek Orion plate and AcroMed Acroplate were fixed on lamb cervical spine.The fixation strength and pull-off strength of the screw-plate constructions was performed initially and after fatigue.Results:Locked CSLP and Orion constructions were more rigid than all unlocked unicortical systems initially and after cyclic loading.There was no significant difference in pull-off strength between the CSLP,the Orion,and the unicortical Acroplate.Conclusions:Locking mechanism significantly increased the strength of the tested screw-plate systems initially and after fatigue.

[Objective]To investigate the ability of repairing bone defect with the combination of recombinant human insulin-like growth factor-1(rhIGF-1),coralline hydrolyapatite(CHA) and autogeneous red bone marrow(ARBM) by way of imaginology.[Method]Bilateral middle radius periosteum-bone defects (11mm in length) were created in 54 Chinese rabbits,and were ramdonly devided into 6 groups(each group containning 18 radial defects of one forearm): group A, defects transplanted with rhIGF-1/CHA/ARBM,group B,with CHA/ARBM,group C,with rhIGF-1/CHA,group D,with CHA,group E,with autograft,group F,no implant.At 2,4,8,and 12 weeks postoperation,the repair effects of defects were evaluated by observation of gross appearance,roengenodiagnosis and radionuclide bone image assay.[Result]In group A,radiological and bone density image analysis showed that the defects were bridged well at 12 weeks postoperatively and was significantly superior to those of any other groups(P0.05).[Conclusion]The recombinant compound rhIGF-1/CHA/ARBM,which possesses the potential ability of osteogenesis,osteocondution and osteoinduction for bone defect repairing,can enhance bone healing and serve as a new type of bone substitute.