Abstract: Objective　To retrospectively analyze the individual dose monitoring of radiation workers in Chongqing City from 2016 to 2020, so as to provide scientific reference for occupational health monitoring and radiation protection. Methods　Radiation workers who performed individual dose monitoring in Chongqing Center for Disease Control and Prevention from 2016 to 2020 were selected as the research objects, and the monitoring data were collected and analyzed. Results　A total of 40 926 individuals were included in this study, with a total of 135 622 person-years. The average annual individual effective dose of radiation workers over the five-year period was 0.56 mSv. NR1 and NR5, the ratio of the number of works receiving annual individual does exceeding 1 and 5 mSv to the to the total monitor workers were 13.019% and 0.335%. The annual effective dose per capita in Chongqing City showed a trend of first decreasing, then increasing and then decreasing again over the study period, with significant differences between different years (F=971.754, P<0.001). Although there were some differences in the variation trend of annual effective dose among different occupational categories, the overall trend was decreasing year by year. The average annual effective dose of nuclear medicine and interventional radiology was relatively high, and the difference was not statistically significant (P>0.05). The average annual effective dose of industrial flaw detection and accelerator operator in industrial application was relatively higher, and the difference was not statistically significant (P>0.05). There were statistically significant differences in the per capita effective doses of different occupational categories of medical application in different years (F=8.892-682.852, P<0.001). Among them, the per capita annual effective doses of five occupational categories of medical application were the highest in 2016, and the per capita annual effective doses of interventional radiology were the highest in 2018 and 2019. Conclusions　The per capita annual effective dose of radiation staff in Chongqing has decreased year by year from 2016 to 2020, and remained at a relatively low level that meets national standards. The monitoring results suggest that the radiation staff of nuclear medicine, interventional radiology, industrial non-destructive testing and accelerator operator should give special attention to protect their occupational health and safety.

Drugs, Chinese Herbal ; therapeutic use ; Humans ; Medicine, Chinese Traditional ; Psoriasis ; diagnosis ; therapy

Objective To compare the difference of wound healing quality of composite skin grafting consisting of xenogeneic (porcine) acellular dermal matrix (PADM) combined with micro-autograft or split-thickness autograft of skin. Methods Seventy-two Sprague-Dawley rats with full-thickness skin defect on the back were randomly divided into three groups,namely test group I with PADM with split-thickness autologous skin,test group II with HADM with autologous micro- skin and allogeneic skin graft,and control group with autologous micro-skin with allogeneic skin graft to cover the skin defect area respectively. The take rate and the condition of wound healing,and also the rates of wound contraction were calculated. Tissue samples were harvested for histological examination. Results The wound contraction was less in test group I and II than that in control group( P

Objective To observe the impact of DPP4 inhibitor Saxagliptin on body weight of type 2 diabetes patients. Methods In this randomized and parallel study,50 patients were given either Saxagliptin(n=25)or Glimepiride(n=24). The changes of body weight, HbA1c and hypoglycemic events were observated in 12 weeks. Results There were no significant difference in gender,age and body weight between 2 groups(P>0. 05). After 12 weeks treatment,body weight has significant changed in both group,and the weight changes were sig-nificant differet in two groups:an average of 0. 4 kg weight was increased in Glimepiride group and 0. 4 kg weight was decreased in Saxagliptin group (P<0. 05). The change of HbA1c level has no statistical difference in two groups (P>0. 05). Hypoglycemia event was happened four times in Glimepiride group,while no hypoglycemia event was happened in Saxagliptin group. Conclusion Compared with Glimepiride,Saxa-gliptin provides similar hypoglycemic action with less hypoglycemia,at the same time,Saxagliptin yields better results in lowering weight.

Objective To explore the effects of 1,25-(OH) 2-D 3 in combined with DBMC to induce tolerance on heterotopic rat cardiac allografts.Methods After the establishment of a stable rat cervical heterotopic heart transplantation model, inbred male Wistar rat hearts were transplanted to male SD rats. The experimental rats were divided into the following groups: Group A served as experimental control group, group B receiving 1,25-(OH) 2-D 3 5000 ng/kg, group C being treated with 1?10 8 DBMC, group D being treated with 1?10 8 DBMC in combined with 1,25-(OH) 2-D 3 5000 ng/kg and group E being treated with CsA 10?mg/kg. The survival time of donor hearts was observed, meanwhile, the donor specific mixed lymphocyte reaction (MLR) and concentrations of TNF-alpha and ICAM-1 in grafts were measured. HE staining inspected all specimens of heart grafts.Results The mean graft survival time (days) in the groups A, B, C, D and E group was 7.2 ? 1.6 , 10.6 ? 2.3 , 12.4 ? 2.5 , 68.3 ? 9.9 and 17.5 ? 4.4 , respectively. The results showed there were significant differences in graft survival time between the group D and the other groups ( P

Objective To observe the effects of different doses of Peng-Hui on expression levels of insulin receptor substance 1 (IRS-1) in type 2 diabetes (T2DM) rats. Methods T2DM rat model was established by feeding with high-fat-diet and intraperitoneal injecting streptozotocin (STZ). T2DM model rats were randomized into four groups: T2DM control group, high dose Peng-Hui (150 mg/kg) group, medium dose Peng-Hui (100 mg/kg) group and low dose Peng-Hui (50 mg/kg) group. The T2DM control group was fed with only high-fat-diet. The normal control group was fed with common diet. The expression levels of IRS-1 in pancreas and skeletal muscle tissues were detected by immunohistochemistry method after 8-week treatment, and fasting blood glucose (FBG) and insulin levels were also detected in five groups. Results FBG lev-els were significantly lower in medium dose Peng-Hui group than those of T2DM control group and low dose Peng-Hui group (P＜0.05). There were no significant differences in insulin levels between high, medium and low dose Peng-Hui groups and T2DM control group (P>0.05). The expression levels of IRS-1 of pancreas and skeletal muscle tissues were significant-ly lower in high dose Peng-Hui group than that of T2DM control group (P＜0.05). Conclusion The expression of IRS-1 was decreased in high dose Peng-Hui group, but no effects on FBG and plasma insulin.

AIM　To analyze the effects of endomorphins (EMs) and their analogs (［D-Pro2］EM-1, ［D-Ala2］EM-1, ［D-Pro2］EM-2 and ［D-Ala2］EM-2) on the cardiovascular system of anesthetized rats and to study its mechanism. METHODS　Responses to EMs and their analogs were investigated in the systemic vascular bed of rats and the mesenteric microarteria of Bufo gargarizans. Responses to EMs were investigated on the hindquarters of the rat vascular bed under constant-flow conditions and on the isolated rabbit thoracic aorta strips. RESULTS　The EMs and their analogs showed dose-related (10-9-10-6 mol*L-1, iv) and naloxone-sensitive (2 mg*kg-1, iv) hypotension in mean arterial pressure of rats with similar duration and vasodilatation in mesenteric microarteria of Bufo gargarizans. The sequence of potencies was EMs > ［D-Pro2］EM-2 > ［D-Ala2］EM-2 > ［D-Ala2］EM-1 > ［D-Pro2］EM-1. EMs were shown not to relax the isolated rabbit thoracic aorta strips without endothelium. EMs, however, relaxed them with endothelium significantly. This action was blocked by Nx (10-5 mol*L-1) and L-NNA (10-4 mol*L-1). CONCLUSION　The significant hypotensive activity of EMs and their analogs is mainly associated with their vasodilatation, which is related to the release of NO from vascular endothelium, and their potency is not completely related to their affinity for μ-opiate receptor.

BACKGROUND: To avoid acute rejection,it is necessary to use imunosuppressive drug regimen for long term to control immune state.However,imunosuppressive drug regimen of allogenic organ transplantation increases infection incidence of recipients,and induction of allograft immunological tolerance might be an ideal method for solving these problems.The long-term immunologic tolerance has been able to be induced in the experimental rodent models.Among these protocols,donor bone marrow cell (DBMC) infusion exerts an important role in the induction of allograft immunological tolerance.OBJECTIVE: To investigate effects of combination of cyclosporine A (CsA) with DBMC infusion on heterotopic rat cardiac allograft survival time.DESIDN: A randomized controlled animal experiment.SETTING: Renal Disease Center,First Affiliated Hospital of Zhejing University School of Medicine.MATERIALS: This study was performed at the Laboratory Animal Center,Zhejiang University School of Medicine between March 2002 and December 2005.Inbred male Lewis rats (n=40,serving as donors) and male BN rats (n=60,serving as recipients) of SPF grade were used in this study.The protocol was approved by the Hospital's Ethic's Committee.METHODS: Forty rats prepared for heterotopic rat cardiac allograft were randomly divided into 4 groups,with 10 rats in each: control group,in which,rats received no treatment,CsA group,in which,rats received CsA infusion for 7 days successively; CsA +DBMC group,in which,rats received DBMCs during and 6 days after the surgery and additional 7 successive days of CsA infusion,and a DBMC group,in which,rats received DBMCs infusion during and 6 days after the surgery.In addition,BN rats that received beterotopic rat cardiac allograft served BN controls.The survival time of heteroropic rat cardiac allograft was investigated.Serum interleukin-2 level and tumor necrosis factor-α mRNA expression level in the transplanted cardiac allograft were measured. The percentage of antigen presenting cells (APC) from donor,CD3+CD25+ cells,CD4+CD25+ cells,CD86+ cells,and the ratio for CD4+CD45RC+ and CD4+CD45RC- in the recipient peripheral blood karyocytes were measured by flow cytometry 6,12 and 18 days after surgery.MAIN OUTCOME MEASURES: The survival time of beteruropic rat cardiac allograft,serum interleukin-2 (IL-2)level,tumor necrosis factor- α (TNF- α ) rnRNA expression level, rejection grading,the percentage of DBMCs in the recipient peripheral blood karyocytes,CD3+CD25+ cells,and CD4+CD25+ cells,as well as CD86 expression,and the ratio for CD4+CD45RC+ and CD4+CD45RC.RESULTS: Forty Lewis male rats and sixty male BN rats were all included in the final analysis. The heterotopic rat cardiac allograft survival time was longer in the CsA +DBMC group than in the control group and DBMC group (P ＜ 0.05). Serum IL-2 level and TNF- α mRNA expression were respectively lower in the CsA +DBMC group than in the control group and DBMC group ( P ＜ 0.05).The rejection was milder in the CsA +DBMC group than in the remaining 3 transplantation groups.In the CsA +DBMC group,CD 86 expression in the recipient peripheral blood karyocytes was markedly inhibited,and 6 and 12 days after surgery,the ratio for CD4+CD45RC+ and CD4+CD45RC- and the percentage of CD3+CD25+ were respectively lower compared to control group and DBMC group.DBMCs in the recipient peripheral blood karyocytes were more in rats that received DBMC infusion compared to rats that received no BDMC infusion.CONCLUSION: Short-term CsA treatment combined with DBMC infusion can lower acute rejection of heterotopic rat cardiac allograft and prolongssurvival time of cardiac allograft.

Bronchi ; drug effects ; pathology ; Cell Line ; Cell Transformation, Neoplastic ; drug effects ; genetics ; Dust ; Epithelial Cells ; drug effects ; pathology ; Gene Library ; Humans ; Minerals ; toxicity

OBJECTIVETo screen the lentiviral vector carrying siRNA and capable of significantly suppressing the ROCK2 gene expression in the corpus cavernosum smooth muscle cells of spontaneously hypertensive rats (SHR).

METHODSWe designed and synthesized 4 siRNA fragments targeting the ROCK2 gene and packaged them into lentiviral vectors. We collected corpus cavernosum smooth muscle cell samples from 5 male SHRs and randomly divided them into groups A (non-transfection control), B (GFP lentiviral transfection), C, D, E, and F (lentiviral transfection with siRNA fragments 1 -4 targeting the ROCK2 gene). Each group consisted of 5 samples and each sample 3 x 10(4) cells. At 48 hours after transfecting MOI = 80 into the SHR corpus cavernosum smooth muscle cells, we detected the expression of GFP under the fluorescent microscope and the mRNA expression of the ROCK2 gene by RT-PCR.

RESULTSThe transfection efficiency of the SHR corpus cavernosum smooth muscle cells was > 50%. Compared with group A, the expression of ROCK2 mRNA in the corpus cavernosum smooth muscle cells showed no remarkable change in group B (P > 0.05) but was inhibited very significantly in C ([43.91 +/- 8.19]%), D ([47.15 +/- 6.64]%), and F ([25.7 +/- 6.03]%) (P < 0.01), and significantly in E ([16.81 +/- 5.94]%, P < 0.05).

CONCLUSIONWe successfully constructed 4 lentiviral vectors carrying siRNA targeting the ROCK2 gene, all of which can significantly suppress the ROCK2 expression in the SHR corpus cavernosum smooth muscle cells, and one has a highly strong inhibitory effect.

Animals ; Gene Silencing ; Genetic Vectors ; Lentivirus ; genetics ; Male ; Myocytes, Smooth Muscle ; Penis ; cytology ; RNA, Messenger ; metabolism ; RNA, Small Interfering ; Random Allocation ; Rats ; Rats, Inbred SHR ; Transfection ; methods ; rho-Associated Kinases ; genetics