Plastic bronchitis has various clinical manifestations and the severity of the disease varies greatly among patients.Extensive lesions tend to threaten the life the patients.The long-term complications and sequelae of plastic bronchitis are related to the severity of the primary disease, treatment methods, treatment time and other factors.Early detection and treatment is one of the important factors to improve the prognosis.

The Ebola virus haemorrhagic fever outbreak began in Guinea and other West Africa countries in Feb.2014.Its high mortality cause worldwide attention.What is Ebola virus? What is its epidemiological characteristics and clinical manifestations? As pediatric medical workers,what should we do? The hot issues at present will be discussed in this article.

Objective To study the clinical value of anatomizing and ligating the short hepatic veins (SHVs) and right hepatic veins through the right Retrohepatic Tunnel (RT) in right hemibepatectomy of giant hepatic carcinoma.Methods We performed the right hemibepatectomy on 23 patients with huge tumors which diameters were lager than 8 cm.There were four main procedures during the operation.Firstly,sperated the interspace between right hepatic veins and middle hepatic veins through secundum porta hepatis.Secondly,seperated and ligated the right hepatic portal vein and hepatic artery in the Glisson's system.Thirdly,anatomized and ligated SHVs and right hepatic veins through the right RT conversly.Finally,set a blocking-belt at the left of the central liver and then cut the right hemi-hepar.Results The anatomy of right hepatic artery,Portal Vein,retrohepatic inferior vena cava (RHIVC),right hepatic veins was well done.The intraoperative blood loss of 7 patients was less than 400 mL,and 11 was 500-700 mL,2 was 800-1 000 mL,3 was 1 100-1 400 mL,and average was 640 mL.The hepatic function was changed in the third day afteroperation.The numerus of TBIL of 16 patients was 20-40 μmol/L,and 6 was 45-50 μmol/L,and 1 was 60 μmol/L.The numerus of ALT of 14 patients was 150-200 U/L,and 9 was 250-400 U/L.The numerus of AST of of 13 patients was 160-200 U/L,and 9 was 230-400 U/L,and 1 was 430 U/L.The numerus of GGT of 14 patients was 160-200 U/L,and 8 was 220-310 U/L,and 1 was 420 U/L.The hepaticfailure happened to 1 patient because of deep venous infection.Conclusions The right hemibepatectomy through the right retrohepatic tunnel is feasible in the tumor without invasion the RHIVC and diaphragm,or the volume was not too huge.The operation could reduce the blood loss and make for the recovery of hepatic funtions.

Carcinoma, Adenoid Cystic ; Female ; Humans ; Laryngeal Neoplasms ; Middle Aged

BACKGROUND: 6-minute walking test (6MWT) is a submaximal test, characterizing by easily processing and low cost, thus it is widely used in the clinic. However, how to change walking distance into maximal motor capacity needs to be further studied.OBJECTIVE: To study the correlation of 6-minute walking distance (D), work (W), peak oxygen uptake (VO_2 peak) and maximal oxygen uptake (VO_2 max) detected by Bruce.DESIGN, TIME AND SETTING: This study was performed at tshe Department of Rehabilitation Medicine, Zhongda Hospital affiliated to Southeast University from March to May 2009.PARTICIPANTS: A total of 25 healthy volunteers were enrolled, including 14 males and 11 females, with a mean age of (22.0±2.3) years.METHODS: All 25 subjects were tested with cardiopulmonary exercise testing (CPET) according to Bruce, VO_2 max and anaerobic threshold (AT) was detected respectively, then 6MWT" was adopted to detect 6-minute walking distance (D), work (W) and maximal oxygen uptake (VO_2 max) of subjects. Data of pulmonary gas exchange in breathing was detected by wireless remote sensor K4b~2 to obtain VO_2 max and VO_2 peak.MAIN OUTCOME MEASURES: ① Regularity of cardiac rate, oxygen uptake and respiratory frequency varied with time passing; ② Walking distance, work, oxygen uptake, cardiac rate and respiratory frequency were compared before and after testing; ③ Maximal oxygen uptake and anaerobic threshold with CPET were compared to peal oxygen uptake with 6MWT; ④ Correlation of distance, work between maximal oxygen uptake and peal oxygen uptake.RESULTS: There was no significant difference between AT from CPET and VO2 peak from 6MWT (P > 0.05). 6-minute walking distance was not correlated with either VO_2 peak or VO_2 max. However, 6-minute walking work was linearly correlated with both VO2 peak (r=0.779 7, P < 0.001) and VO_2 max (r=0.894 1, P < 0.001).CONCLUSION: 6-minute walking test is an exercise test at level of anaerobic threshold. 6-minute walking work can reflect both submaximal exercise capacity and maximal exercise capacity.

Clinical data of 150 patients with mild and 38 patients with severe confirmed influenza A/H1N1 were collected and retrospectively analyzed in this paper with descriptive epidemiology. Mild patients mainly presented with symptoms similar to seasonal influenza with few complications, however,persistent high fever, cough with bloody sputum expectoration, chest distress and short of breath manifested in severe patients with respiratory failure and acute respiratory distress syndrome in some cases. Proportion of obesity and underlying diseases in severe patients, who were at high risk for severe influenza A/H1N1, was higher than that in mild ones (P＜0. 05). Average course of the illness spanned five to 11 days in patient with mild influenza A/H1N1 with all cured, and 34 of severe cases discharged with better recovery and other four died with a case-fatality of 10. 5 percent.

Magnetic resonance imaging has shown its important role in both early diagnosis of Alzheimer's disease and revealing the Alzheimer's disease pathogenesis.Structural magnetic resonance imaging include lineal mesure,volume measure and volume rendered.They can detect the abnormal brain structure,especially the change of mesial temporal lobe(hippocampus,amygdale) in early onset of Alzheimer's disease.Recent studies more focused on functional imaging which can find the functional changes in brain.Prior to structural changes,functional changes happen,so it can reflect the sutile pathological process in Alzheimer's disease.The functional imaging mainly refers to magnetic resonance spectroscopy,functional magnetic resonance imaging,diffusion weighted imaging and diffusion tensor imaging.As developing of these new technologies,we know more and more about pathogenesis and biological characteristics of Alzheimer's disease.

HIV-related sensory neuropathy ( HIV-SN) mainly contains the HIV infection-related distal sensory polyneuropa-thy (DSP) and antiretroviral toxic neuropathies (ATN).HIV-DSP is associated with proinflammatory cytokines , chemokines, ros and which is induced by gp 120;and HIV-ATN may be related to mitochondrial toxicity which is induced by the application of anti retroviral drugs, such as ddC, and similar as the molecular mechanism of HIV-DSP, this means that the current conventional method for the treatment of neuropathic pain in AIDS may further aggravate the neuropathic pain of the patient .Therefore, developing the study on the neurochemical and pharmacological mechanisms of HIV-related neuropathic pain will provide novel targets for the new effective drugs .

Objective To compare the inhibition effect of budesonide suspension and dexamethasone on the rats′ pneumonic anaphylacticitis and serum IL-5,and study the effect mechanism in treatment of asthma with glucocorticoids on systemic administration and with budesonide suspension.Methods Twenty-six health wistar rats were randomly divided into normal group,asthmatic group,budesonide suspension group and dexamethasone group.Asthma models were sensitized with egg albumin.Before sensitized,budesonide suspension group were administered inhalation of budesonide suspension,and dexamethasone group were administered abdominal injection of dexamethasone.The level of IL-5 was measured with enzyme linked immunosorbent assay(ELISA) reagent box,lung was eviscera-(ted after collecting) blood,pathological slice was made.Results The rats′ pathological changes of lung and bronchus were significant between asthma group and other groups,while there were no significant difference between budesonide suspension group and dexamethasone group,but both of two groups showed slight difference with normal group.Compared with normal group,the level of serum IL-5 in asthma group raised obviously,and there was significant difference(P0.05).Conclusions The treatment of inhalation of budesonide suspension can inhibit rats′ pneumonic anaphylacticitis,ameliorate rat anaphylactic asthma symptom.

EC-SOD inclusion bodies was refolded on column using metal (Ni) affinity chromatography, based on the metal-binding property of His-tag. The effect of protein amount, urea removal speed and temperature on refolding was observed. We compared the different efficiency purified with Ni-sepharose and Heprain-sepharose affinity chromatography, and studied the stability of the refolded proteins. The results indicate that the inclusion bodies can be renatured with Ni-sepharose affinity chromatography. The increase of the protein amount and urea removal rate , the lower of the renaturation efficiency. Higher temperature was benefit to protein renaturation. Both the Ni-sepharose and Heprain-sepharose affinity column can be used to purified the refolded proteins, but purified by Heprain-sepharose affinity column the protein had higher activity. The activity of renatured protein was stable in 10 ℃～50℃,when pH10 its stability was lower significantly. In denaturating solution the stability of renatured protein was low.