Acromioclavicular Joint ; anatomy & histology ; injuries ; surgery ; Fracture Fixation ; instrumentation ; methods ; Humans ; Male ; Shoulder Dislocation ; surgery

Breast Neoplasms ; diagnosis ; China ; Female ; Humans ; Mass Screening ; standards

Gangliosides are sialic acid-containing glycosphingolipids and abundant in the brain. Some studies showed that glycosphingolipids not only could improve the differentiation of neurons, neurite outgrowth and synaptogenesis, but also could modulate neuronal plasticity and improve neurological function after cerebral damage. GM1 was so far most widely studied glycosphingolipids. It is believed that the regulatory functions for calcium homeostasis and the interaction between GM1 and neurotrophins are essential in the mechanisms underlying its neuroprotection. In addition, GM1 has also anti-excitotoxicity, anti-oxidation and vasodilation properties. Various forms of chronic neurodegenerative diseases and hypoxia/ischemic encepha-lopathy are characterized by progressive loss and apoptosis of neurons, the neuroprotective and neruotrophic effects of GM1 could play pivotal role in the treatment of these diseases. Nowadays, GM1 was widely used for the treatment of Parkinson's disease, stroke, hypoxia-ischemic encephalopathy, craniocerebral trauma, spinal cord injury and peripheral neuropathy. Further researches for the mechanisms of GM1 will provide new strategies for the treatment of central nervous system diseases.

Objective To explore the cause of anastomotie fistula after esophagus cancer and cardiac cancer operation and its prevention and treatment.Methods After excising the tumors,the esophagogastrostomy was performed in 60 cases,in which the cervical anastomose in 48 cases and intrathoracic anastomose was adopted in 12 cases.We retrospectively analyzed its prevention and treatment. Results Caevial anastomose in 48 cases,4 patients with anastomotic fistula in the died,while none of 12 cases in the cervical anastomose died. Conclusions Careful procedure and improving the anastomotic methods are the key factors to reduse the anastomotic fistula,the accurate control of infection and full nutritive support are the effective methods to decrease the mortality rate of this disease.

Objective 　To evaluate curative effect of Quhuang Tieji on hypercholesterolemia of newborn. Method　120 newborn with hypercholesterolemia were randomly divided into treatment group and control group,two group all accepted routine treatment,but the former group was treated with Quhuang Tieji at the same time.NANA and BAER were measured before and after treatment in two group.Results 　The scores of NBNA,latency of V wave,Ⅲ-V wave internal were significantly different between treatment group and control group after treatment.Conclusion　Quhuang Tieji can improve the scores of NBNA and latency of BAER , it had well curative effect to hyperbilirubinemia of newborn.

Pediatric liver transplantation has long been developed in the western world.Currently,favorable outcomes after liver transplantation have been achieved in pediatric recipients and the postoperative 5-year survival rate reached 80％ in western transplantation centers.In the mainland China,pediatric liver transplantation started quite recently and there is still a big gap compared with western centers.In addition,there is unbalanced development between adult and pediatric recipients due to surgical difficulties and undesirable outcomes following pediatric liver transplantation.The operation methods of pediatric liver transplantation include whole liver transplantation,reduced-size liver transplantation,living donor liver transplantation and split liver transplantation.Perioperative complications of pediatric liver transplantation mainly include portal vein thrombosis,hepatic artery thrombosis,intra-abdominal bleeding,infections,rejection,bile leakage and biliary strictures.While the long-term complications are infections,post-transplantation lymphoproliferative disorders,acute and chronic rejections caused by poor compliance with immunosuppressive therapy.Perioperative complications have been reduced recently thanks to improvement of surgical techniques.With the highly-prolonged survival period,long-term postoperative complications have been playing a significant role in recipients' survival rates.Management of long-term follow-up and compliance has been the next focus of pediatric liver transplantation.

This paper describes photobiological hazards and the standards of photobiological safety in the application of medical devices. It analyzes and discusses the current status of the international standardization and the national standardization.
Equipment and Supplies ; standards ; Photobiology ; Safety

Objective:To develop the method of detecting the level ofHey1 expression_the target gene of Notch signaling pathway in bone marrow mesenchymal stem cells,using real-time fluorescence quantitative RT-PCR.Methods:The bone marrow mesenchymal stem cells (C2C12) were cultured and the total RNA was extracted.And then the amplification efficiency was verified between the reference gene ?-actin and the target gene Hey1.To optimize the PCR conditions and analyse amplification curves and melting curve before validation of quantitatve PCR products with gel electrophoresis.Results:The reference gene?-actin and the target gene Hey1 have the same amplification efficiency(slope difference of the amplification curve

Objective:To establish a solid phase extraction(SPE)and reversed-phase high-performance liquid chromatographic (RP-HPLC)method for the determination of tetrazanbigen in liver.Methods:Tetrazanbigen in liver samples was determined after a C18 solid phase extraction on a Lichrospher C18 column at 260 nm with an isocratic mobile phase of 90%methanol in 100 ml methanol -deionized water(pH 6.5),containing 0.1%triethyl amine.The flow rate was set at 1.0 ml/min.Results:The linearity was good over the concentration range of 2～20?g/ml(r=0.9 993).The average recovery and RSD were 106.47%and 3.0%respectively.The lower limit of quantification was 1.0?g/ml.Conclusion:The method is simple,accurate and can be used to determine the TNBG concentration in liver and study its metabolism.

Objective:To explore the diagnostic value of ABI detection in lower limb vasculopathy of diabetic patients.Methods:ABI detection was performed in 44 cases suspected of diabetic lower limb vasculopathy,and the results were compared with that of DSA. Results:There was no statistically significant difference between ABI detection and DSA in the diagnosis of diabetic lower limb vasculopathy.Compared with DSA,the accordance rate,sensitivity and specificity of ABI were 87.31%,86.46%and 82.00%respectively. Conclusion:By ABI detection,lower limb vasculopathy of diabetic patients can be detected.