Chronic Disease ; Constipation ; diagnosis ; drug therapy ; Evidence-Based Medicine ; Humans ; Integrative Medicine

Objective To observe the rat tracheal stem cells in situ.Methods Tracheal rings of Wistar rats treated with 5-FU to make an injure model in vitro then observing the process of regeneration in sequence time from 3*!hours to 48*!hours. Use light microscope to detect the development of tracheal epithelium,assay the level of proliferating cell nuclear antigen (PCNA) immunohistochemistry and perform Hoechst33342 staining to search the negative staining cells. Results Twelve hours after 5-FU it could be seen that tracheal mucosa exfoliated with nuke-nucleus like cells nailed just above the basement membrane,6*!hours after removing 5-FU we could see that the tracheal rings were covered with squamous epithelium. It could be seen that PCNA immunohistochemistry negative staining cells located bordering on the positive one. Meanwhile under fluorescent contrast microscope negative cells with no Hoechst dye combinations were among most of the positive cells. 12*!hours after removing 5-FU mucous granular cells and cilia cells could be seen. After 48*!hours the whole tracheal rings were covered with pseudostratified columnar ciliated epithelium.Conclusion 5-FU is a cell-cycle specific antimetabolite cytotoxicant.It can make the cycling cells degeneration and necrosis but has little effect on the G 0 cells. Few G 0 cells which look like nuke nucleus cells randomly on the basement have the ability to efflux the dye Hoechst33342.The tracheal rings are regenerated through these cells differentiation and proliferation.

Objective To study the effect of fetal transplants on the fu nctional recovery after spinal cord transection in new born and adult rats. Meth ods The spinal cord tissue at E14 of a rat fetal was transferred into the hemise ction cavity at lumbar spinal cord in the newborn and adult rats. 4, 8, 12 weeks after the operation, spinal cord tissue morphology, combined behavioral score ( CBS), somatosensory evoked potentials (SEP), and motor evoked potentials (MEP) w ere examined. Results The transplanted tissue survived in the host spinal cord. There was a statistical difference in CBS between the newborn graft group and th e adult graft group. The peak latencies of early waves in SEPs and MEPs of the n ewborn graft group were superior to those of the adult graft group (P

Spinal cord injury occurs with an average annual incidence of 15 40 cases per million of population. The costs of treatment and rehabilitation in the living period of patients with spinal cord injury are very high. Prevention, therapy and rehabilitation of spinal cord injury have become a major subject in medical science. The aim of this article is to summarize the statistics of incidence, etiological factors, clinical characteristics and complications in patients with spinal cord injury.

The incidence rate of thyroid cancer is increasing very rapidly during the past years.131I treatment for DTC is an effective method.However,DTC refractory to 131I treatment or therapeutic failure is not uncommon.High level expression of nuclear factor-kappa B (NF-λB) in thyroid cancer is closely related with carcinogenesis,progression,anti-apoptosis and therapeutic resistance.NF-κB inhibitor was effective for the treatment of thyroid cancer.Combined NF-κB inhibitor with131I may improve the therapeutic efficacy.

As a distinct T cell subset with acknowledged specific function and marker, CD4+CD25+FOXP3+ regulatory T cells are thought to dampen T-cell immunity and to be the main obstacle tempering antitumor immunotherapy. Accumulating evidence has confirmed an increased pool of regulatory T cells both in the peripheral blood and the tumor microenvironment of cancer patients, which are indicative of disease progression, response to therapy, invasive phenotype and prognosis. Therefore, manipulation of regulatory T cells—including depletion, blocking trafficking into tumors, or reducing their differentiation and suppressive mechanisms—and concomitant stimulation of effector T cells, systemically or locally in tumors, represent new strategies for cancer treatment.

Objective:To explore therapeutic effect of repaglinide on patients with type 2 diabetes mellitus (T2DM) complicated atherosclerosis (AS).Methods:A total of 120 T2DM + AS patients treated in our hospital from Mar 2012 to May 2014 were selected.They were randomly and equally divided into repaglinide group (received repaglin- ide treatment)and glipizide group (received glipizide treatment).Fasting blood glucose (FBG)and 2h postprandial blood glucose (2hPG)levels before and after treatment,serum levels of high sensitive C reactive protein (hsCRP) and oxidized low density lipoprotein (oxLDL)after treatment,and patients'satisfaction on treatment were com- pared and analyzed between two groups.Results:Compared with before treatment,there were significant reductions in levels of FBG and 2hPG in both groups after treatment (P<0.01 all);compared with glipizide group,there were significant reductions in levels of FBG [(6.94±0.41)mmol/L vs.(6.05±0.54)mmol/L],2hPG [(8.03±0.74) mmol/L vs.(7.66±0.78)mmol/L],serum hsCRP [(5.75±0.26)mg/L vs.(4.71±0.32)mg/L]and serum ox- LDL [(98.30±25.05)μg/dl vs.(52.21±17.50)μg/dl]after treatment in repaglinide group,P<0.01 all.Satis- faction rate in repaglinide group was significantly higher than that of glipizide group (93.33% vs.60.00%),P<0.01. Conclusion:Repaglinide possesses significant therapeutic effect on patients with type 2 diabetes mellitus com- plicated atherosclerosis.It can significantly reduce blood glucose,blood lipid and serum hsCRP levels.

AIM: To study the effects of angiotensin converting enzyme inhibitor(ACEI) on elastase after balloon injury. METHODS: The carotid arteries and aortas twelve-weeks-old Wistar male rats were injured by balloon catheter. The rats were divided into experimental and control groups in which ACEI (temocapril-HCl,10 mg?kg -1 ?d -1 ) and the vehicle were administered 2 days before injury respectively and the animals were sacrificed on day 2, 3, 5 and 10, respectively. In situ hybridization, immunohistochemistry and elastase activity bioassay were used for studying elastase . RESULTS: The intimal area on day 10 in the experimental group was significantly suppressed compared to that in the control rats( P

ObjectiveTo evaluate the effect of ultrasound guided pus aspiration for appendiceal abscess.MethodsPercutaneous puncture and pus aspiration was performed under guidance of B US on 181 patients with appendiceal abscess, result was compared with 23 patients treated conservatively.ResultsCompared with that of control group, in pus aspiration group abdominal pain lasted 5 5 days shorter,fever lasted 5 days shorter,tenderness did 6 days shorter, cost was 2?356 yuan less and hospital stay shortened by 6 days.ConclusionPus aspiration under B US guidance is a safe,simple,less cost and effective treatment for appendiceal abscess larger than 1?cm?2?cm in children patients.

The poverty population definition for medical assistance was related to equity and precision,but still lacked theory and method in practice.The catastrophic and impoverishment health expenditure methods based on relative costs theory considered the family's ability to pay,which reflected the economic burden and poverty status of families.Meanwhile,they reflected the breadth and depth of poverty.Therefore,the theory and methods of catastrophic diseases relative costs could support the definition of catastrophic diseases for poverty population in China.