Objective: To screen for differentially expressed genes associated with the development and progression of human growth hormone adenoma, so as to lay a foundation for future study. Methods: The whole genome oligonucleotide microarray (Affymetrix 133 plus 2.0) was used to examine gene profiles of 8 growth homone adenoma samples and 2 normal pooled pituitary samples. Differentially expressed genes were subjected to bioinformatics analysis. Real-time qPCR was used to verify the microarray result of a randomly selected candidate gene. Results: A total of 187 up-regulated genes and 899 down-regulated genes associated with growth hormone adenoma were screened out, with their functions mainly associated with molecular binding, apoptosis/tumor, metabolism, signal transduction, cell cycle, and transportation activities. Conclusion: Microarray technology can be used for preliminary screen of growth hormone adenoma associated genes. The development and progression of growth homone adenoma are complex processes involving multiple genes, molecules, and pathways.

Purpose To explore the expression of two epithelial-mesenchymal transition (EMT) regulators,Sry-related high mobility group box 4 (SOX4) and enhancer of zeste homolog 2 (EZH2),in breast cancer tissues and their clinicopathologic significances.Methods The expression of SOX4 and EZH2 was detected by immunohistochemistry of EnVision twostep in paraffin-embedded tissue sections from 241 cases of breast cancer and 126 cases of paracancerous breast tissues.The relationship between their expression and clinicopathologic characteristics was statistically analyzed,and their correlation was also analyzed.Results In breast cancer tissues and paracancerous breast tissues,the high expression rates of SOX4 were 82.2％ and 57.1％ respectively,while the high expression rates of EZH2 were 80.1％ and 47.6％ respectively.Their expression in breast cancer tissues was higher than that in paracancerous breast tissues (P ＜ 0.05).High SOX4 expression was correlated with lymph node metastasis,high TNM stage,and overexpression of HER-2 (P ＜ 0.05),while high EZH2 expression was correlated with high histological grade,lymph node metastasis,ER negativity,EGFR positivity and high poliferation index of Ki67 (P ＜ 0.05).There was a positive correlation between SOX4 and EZH2 expression in breast cancer tissues (rs =0.256,P ＜0.001).Conclusion EMT regulators SOX4 and EZH2 might be responsible for the metastasis of breast cancer,which are upregulated in breast cancer tissues.They may act as potential biomarkers to predict the metastasis of breast cancer.

Objective To investigate the curative effect of glucocorticoid and hyperbaric oxygen therapy on the high-frequency and diminishing sudden deafness.Methods A total of 260 patients with high-frequency and diminishing sudden deafness were divided into hormone group(n=110),hyperbaric oxygen group(n=80) and routing group(n=70,ginaton 87.5 mg+5% GS 250 mL static drops static drops,intramuscular injection of vitamin B1 1.5 mg+cobamamide 0.1 g and 2% lidocaine 10 mL+5% GS 250 mL static drops).The first two groups were added oral prednison and hyperbaric oxygen therapy on the basis of therapy of routing group.The effect of comparison analysis was conducted after 10 days of treatment according to the course of disease which was less than that of or equal to 7 days or greater than 7 days.Results The overall curative effect of hormone group and hyperbaric oxygen group was better than that of routing group,and the difference was statistically significant (P<0.05).During the whole course of disease,the difference of therapeutic effect between routing group and the other two groups was statistically significant(P<0.05).Conclusion The glucocorticoid and hyperbaric oxygen therapy both have really curative effect on high-frequency and diminishing sudden deafness.

Objective To study the influence of bFGF gene transfected bone marrow-derived mesenchymal stem cells (BMSCs) on the inflammatory cytokines of COPD rat. Methods The BMSCs were separated from SD rat and cultured and then bFGF gene was imported to BMSCs by liposome transfection method. The samples were prepared into six groups: normal control group, COPD group (A), BMSCs group (B), pcDNA3.1-BMSCs group (C), bFGF-pcDNA3.1-BMSCs group (D), and bFGF group (E). The expressions of TNF-α and IL-1β by QRT-PCR were detected. Results Compared with COPD group, TNF-α and IL-1β genes from groups B to D dropped significantly (P < 0.05). The changes of TNF-α and IL-1β genes among groups B to D showed no significant difference (P > 0.05). Conclusion BFGF transfected BMSCs, sample BMSCs and pcDNA3.1 transfected BMSCs can inhibit the expression of inflammatory cytokines of TNF-α and IL-1β, but there is no obvious advantage in comparison to bFGF transfected BMSCs and sample BMSCs in respect of inhibiting the expression of inflammatory cytokines of TNF-α and IL-1β.

Objective: To systematically investigate the prevalence of anxiety among healthcare professionals during the COVID-19 pandemic, so as to provide the development of evidence-based psychological interventions among healthcare professionals. Methods: The publications pertaining to the prevalence of anxiety among healthcare professionals during the COVID-19 pandemic were retrieved in national and international electronic databases from January 1, 2020 through November 30, 2021, including CNKI, Wanfang Data, VIP, SinoMed, PubMed and Web of Science. The quality of publications was evaluated using the United States Healthcare Research and Quality (AHRQ) quality assessment of included cross-sectional studies, and the pooled prevalence of anxiety was estimated among healthcare professionals using the software Open Meta Analyst version 3.0. The publication bias were evaluated with funnel plots and Begg rank correlation test. Results: Totally 598 publications were retrieved, and 36 eligible publications were enrolled in the final analysis, including 33 Chinese publications and 3 English publications. There were 5 high-quality, 29 moderate-quality and 2 low-quality publications. All investigations pertaining to the prevalence of anxiety among healthcare professionals were conducted in 2020. Totally 19 872 healthcare professionals were investigated, and the prevalence of anxiety was 28.8% (95%CI: 24.0%-33.6%). Subgroup analysis showed that the prevalence of anxiety was 31.9% (95%CI: 17.6%-46.2%) among healthcare professionals from western China, 29.6% (95%CI: (17.8%-41.4%) from central China, and 25.3% (95%CI: 20.2%-30.3%) from eastern China. The prevalence of anxiety was 4.9% (95%CI: 3.3%-6.4%) among male healthcare professionals and 22.9% (95%CI: 17.7%-28.0%) among male healthcare professionals, and the prevalence of anxiety was 21.6% (95%CI: 13.2%-29.9%) among nurses, 5.2% (95%CI: 2.8%-7.5%) among doctors and 4.8% (95%CI: 2.2%-7.4%) among other healthcare professionals. The prevalence of mild, moderate and severe anxiety was 18.6% (95%CI: 14.0%-23.2%), 5.5% (95%CI: 4.1%-6.8%) and 1.9% (95%CI: 1.3%-2.5%), respectively. No publication bias was detected as revealed by funnel plots and Begg rank correlation test, and stable meta-analysis results and heterogeneity test were observed. Conclusions The prevalence of anxiety is 28.8% among healthcare professionals during the COVID-19 pandemic, and mild anxiety is predominant. A high prevalence rate of anxiety is seen female healthcare professionals and nurses, who should be given a high priority and timely psychological interventions

Child ; Endoscopy ; Humans ; Male ; Paraganglioma ; surgery ; Urinary Bladder Neoplasms ; surgery

OBJECTIVE:To observe clinical efficacy of bevacizumab or cetuxizumab combined with FOLFOX4 regimen in the treatment of advanced rectal cancer. METHODS:114 patients with rectal cancer were randomly assigned to cetuxizumab group and bevacizumab group,with 57 cases in each group,among which one patient of bevacizumab group withdrew from therapy. Both groups received FOLFOX4 regimen:oxaliplatin 85 mg/m2+calcium folinate 200 mg/m2,ivgtt,2 h,and 5-FU 400 mg/m2,ivgtt, last,5-FU 600 mg/m2,ivgtt,22 h. Cetuxizumab group was additional given cetuxizumab 500 mg/m2;bevacizumab group was addi-tionally given bevacizumab 5 mg/kg,ivgtt. A treatment course lasted for 2 weeks. Both groups received 4 courses of treatment,and then clinical efficacy,toxic reaction and progression-free survival (PFS) were evaluated. RESULTS:Objective remission rate (RR),disease control rate(DCR)and median PFS of cetuxizumab group was 45.61%,92.98%and 10.0 months,those of bevaci-zumab group were 48.21%,87.50%and 11.0 months;there was no statistical significance between 2 groups(P>0.05). No signifi-cant differences were found in the incidence of ADR such as sensory neurotoxicity,aleucocytosis,thrombopenia,nausea and vomit-ing,diarrhea and erythra between 2 groups(P>0.05). CONCLUSIONS:Both bevacizumab or cetuxizumab combined with FOLF-OX4 regimen have a similar effect on patients with advanced cancer,with low incidence of toxic reaction.

Electricity can stimulate cerebral fastigial nucleus to increase regional cerebral blood flow (rCBF) and narrow the volume of infarct, therefore its effect on ischemic brain disorder may be helpful. The therapeutic instrument for brain circulation function based on direct digital synthesis (DDS) technology uses the domestic newest scientific findings and others in foreign countries. Adopting the DDS technology directly, it institutes safe and efficacious treatment. Through pasting the electrodes on body surface, the non-invasive instrument introduces electric current to cerebral fastigial nucleus (FN) and hence it improves the state of brain blood supply and nerve conduction, increases cerebral blood flow, reduces the number of necrotic neurons in the district of penumbra, and mitigates brain edema. Consequently, it improves the function of brain circulation.
Brain Ischemia ; physiopathology ; therapy ; Cerebrovascular Circulation ; physiology ; Computers ; Electric Stimulation Therapy ; instrumentation ; methods ; Equipment Design ; Humans ; Vasodilation

Objective To understand the status of drinking-water supply and the progress of waterimproving projects in drinking-water-borne endemic fluorosis areas in Henan,and to provide scientific data for related government departments to carry out prevention and surveillance plan for those areas.Methods Questionnaire survey was carried out in all the villages in drinking-water-borne endemic fluorosis areas in Henan in 2010.Each village was given one set of questionnaire.Results By the end of 2010,the total number of fluorosis villages in Henan was 25 434,among them 11 484 villages had been conducted water-improving projects,accounting for 45.15％.Currently,9267 water-improving projects worked properly,accounting for 36.4％(9267/25 434)of all villages surveyed,and 80.7％ (9267/11 484)villages had water-improving projects.The projects in 2217 villages do not work properly,accounting for 19.3％(2217/11 484) of all projects.There were 5832 water-imp.roving projects conducted amnog 2005-2010,accounting for 50.8％ (5832/11 484) of all water-improving projects.Among villages with no such projects,97.5％(15 769/16 167) of them used shallow groundwater,and in villages with water-improving projects,89.6％ (8303/9267) of them used deep groundwater as drinking-water supply.Conclusions After 2005,the water improving progress was significantly speed up in drinking-water-borne endemic fluorosis areas in Henan,but progress of the water improving projects is relatively slow due to changing and expanding of fluorosis areas.The government should increase investment and improve the proportion of waterimproving defluoridation.

ObjectiveTo study the distribution,location and hemodynamics of perforating branches of the ulnar artery with color Doppler flow imaging (CDFI).MethodsPerforating branches of ulnar arteries were examined in 80 healthy volunteers in both forearms using CDFI.Following parameters were detected:number,diameter,jumping-off point,course,location and peak blood velocity.All the parameters data were analyzed statistically.ResultsThree hundred and forty-five perforating branches were detected in total 160ulnar arteries.These branches were classified into three types:type Ⅰ,myocutaneous perforator (10.3 ％);type Ⅱ,septocutaneous perforator(87 ％ ) ; type Ⅲ,direct cutaneous perforator (2.7 ％ ).The most dominant branches were located in the mesial of the upper third,middle third and lower third of the forearm.There was no significant difference among all age groups and no difference in peak blood velocity between left side and right side( P ＞0.05).The peak blood velocity was higher in male than that in female.( P ＜0.05).In relaxing period,the blood flow rate was almost disappeared and the frequency spectrum showed the characteristics of single direction,lower blood velocity and higher blood resistance.ConclusionsCDFI with high resolution showed better results of distribution and location of perforating branches of the ulnar artery and a better quality evaluation of the these branches.CDFI was helpful to design the skin flap containing cutaneous perforators of ulnar artery.