Chondroblastoma of the patella, rare occurred in patellar,is a kind of an uncommon benign bone tumor. Compared with giant cell tumor, the morbidity of chondroblastoma is lower. Meanwhile, its clinical manifestations are various, and images are very complicated. Therefore, the understanding of this kind of tumors may be limited even to the orthopedist. The differences of patellar chondroblastoma between other tumor in X-ray, CT and MRI is a spot in recent years. Sometimes patellar chondroblastoma coexists with aneurysmal bone cyst, which is a challenge to obtain an accurate pathological and radiological diagnosis. For the treatment, curettage and bone grafting is one the most popular method, but whether to perform a biopsy before surgery still remain controversy. Some new technique still has an unknown prospect for the treatment such as radiofrequency ablation.
Bone Neoplasms ; diagnosis ; surgery ; Chondroblastoma ; diagnosis ; surgery ; Humans ; Patella ; surgery

Objective: To study correlation between plasma mitochondrial coupling factor-6 (MCF-6) level in coronary circulation and coronary stenotic degree in CHD patients. Methods: A total of 64 CHD patients were selected as CHD group, and they were further divided into single-vessel group, double-vessel group and multi-vessel coronary disease group; another 20 cases with normal coronary artery were enrolled as normal control group. Radioimmunoassay method was used to measure plasma MCF-6 concentration in peripheral, coronary sinus and aortic root in each group. Multivariant linear gradual regressive analysis was used to analyze above-mentioned correlation. Results: Compared with normal control group, there was significant increase in plasma MCF-6 concentration in peripheral, aortic root and coronary sinus in CHD patients (P<0.05 all) ；compared with aortic root and peripheral plasma, there was significant increase in MCF-6 concentration [(348±48) pg/ml vs. (340±51) pg/ml vs. (402±56) pg/ml, P<0.01 all] in coronary sinus of CHD group；Compared with single-vessel group, double-vessel group, there were increase in MCF-6 concentration in multi-vessel coronary disease group (P<0.05～0.01); Multivariant linear gradual regressive analysis indicated that Gensini score was significant positively correlated with plasma MCF-6 concentrations in coronary sinus and aortic root (r=0.650, P<0.01; r=0.711, P<0.01). Conclusion: Mitochondrial coupling factor-6 participates in the pathophysiological process of coronary heart disease, which may be an important vasoactive substance during development of coronary heart disease.

Objective To explore the effects of gamma-aminobutyric acid (GABA) on the proliferation, apoptosis and invasion of cholangiocarcinoma cell line QBC_(939). Methods Cholangiocarcinoma cell line QBC_(939) was cultured via routine methods, and was treated with different concentrations of GABA (1, 10,100, 1000 μmol/L, test group) or with complete culture medium (control group). The effects of GABA on the proliferation, apoptosis and invasion of cholangiocarcinoma cell line QBC_(939) were investigated by MTT assay, flow cytometry and Transwell cell culture chamber assay, respectively. The effects of GABA on the activity of matrix metalloproteinase (MMP) secreted by cholangiocarcinoma cells were analyzed by gelatin zymogram, and the content of intracellular cyclic adenosine monophosphate (cAMP) was measured by radioimmunoassay. All data were analyzed by one-way ANOVA. Results As the GABA concentration increased from 1 μmol/L to 1000 μmol/L, the inhibition rate of GABA on cholangiocarcinoma cell line QBC_(939) was increased from 2.6% to 26.8%, and the apoptosis rate also increased from 4.80%±0.04% to 28.03% ±0.01%. The number of cholangiocarcinoma cells that migrated through the Matrigel gel decreased from 60±10 to 43±4, in a dose-dependent manner (F = 7. 883, 83.765, 7. 598, P <0.05). The activity of MMP-2 and MMP-9 was decreased. The content of intracellular cAMP was increased, and there was significant difference in the content of intracellular cAMP between test and control groups (F =9.507, 9.148, 27.418, P < 0.05). Conclusions GABA can inhibit the proliferation and invasion of cholangiocarcinoma cell line QBC_(939) cells by promoting the apoptosis and inhibiting the activity of MMP-2 and MMP-9. The process may be mediated by the information transmission of post-receptor.

Objective To explore the effect of GABA on the bionomics of cholangiocarcinoma cell QBC939. Methods The proliferation of QBC939 cells was investigated by MTT. The telomerase activity of QBC939 ceils was examined by modified PCR-ELISA assay. Transwell cell culture chamber assay in vitro was used to detect the ability of invasion of cholangiocarcinoma QBC939. The expression of matrix metalloproteinase (MMP) mRNA and enzymatic activity of QBC939 cells were detected by reverse transcription polymerase chain reaction(RT-PCR) and gelatin zymography, respectively. Results GABA at all tested concentration inhibited the growth and proliferation of QBC939 cells in a time-independent manner, Telomerase activity was inhibited by GABA [(0.82 ± 0.05) vs. (0.56 ± 0.05), (P<0.05)]. GABA inhibited tumor cells' trans-Matrigel ability (at 100 μmol/L GABA, cell's number decreased from 60 ± 10 to 43 ±4, P<0.05) ,and significantly inhibited the activity and expression of MMP(MMP-2、MMP- 9) in a dose dependent way. Conclusions GABA can inhibit the growth、invasion and metastasis of cholangiocarcinoma QBC939. The down-regulation of the activity of Telomerase and MMP may be involved inthis mechanism.

Ductus Arteriosus ; surgery ; Ductus Arteriosus, Patent ; drug therapy ; therapy ; Humans ; Ibuprofen ; administration & dosage ; therapeutic use ; Indomethacin ; administration & dosage ; therapeutic use ; Infant, Low Birth Weight ; Infant, Newborn ; Infant, Premature ; Infant, Premature, Diseases ; drug therapy ; surgery ; therapy ; Ligation ; methods ; Treatment Outcome

BACKGROUND:There have been many studies on the diagnosis of osteoporotic vertebral compression fractures. However, there are few studies on the diagnostic methods of fracture in different periods. OBJECTIVE:To comparatively analyze the application value of high-intensity magnetic resonance imaging (MRI) and single photon emission computed tomography combined with CT scan image fusion (SPECT-CT) in the diagnosis of osteoporotic vertebral compression fractures. METHODS:Clinical data of 35 patients with osteoporotic vertebral compression fractures were retrospectively analyzed, including 56 vertebrae. There were 14 males and 21 females, with an average age of 72.6 years. The course of disease was within 3 weeks in 22 patients, belonging to acute fractures. The course of the disease was between 4 and 12 weeks in 10 patients, belonging to the subacute fractures. The course of disease was 6 months in 3 patients, belonging to the fracture healing period. Al patients were tested with high-intensity MRI and SPECT-CT examination, and were treated with vertebroplasty or percutaneous kyphoplasty. The Fisher exact method was used to compare the two diagnostic methods in the diagnosis of vertebral fracture. RESULTS AND CONCLUSION:(1) In the 56 responsible vertebral bodies, 32 vertebral bodies belonged to the acute-stage fractures, and 24 vertebral bodies belonged to the non-acute-stage fractures. In al the fractures, MRI diagnosed 49 segments;SPECT-CT diagnosed 52 segments. The sensitivity of SPECT-CT testing was higher than MRI, and its specificity was less than MRI. There was a high consistency of diagnosis between fractures. (2) In the 32 acute vertebral fractures, the two tests diagnosed 29 segments. The sensitivity of SPECT-CT was higher than that of MRI, but its specificity was lower than that of the MRI. There was a high consistency between the diagnoses of fractures. (3) In the 24 non-acute vertebral fractures, MRI diagnosed 20 segments;SPECT-CT diagnosed 23 segments. SPECT-CT was more sensitive than MRI;the specificity of SPECT-CT was lower than MRI. There was a high consistency between the two diagnoses of fractures. (4) There was a high consistency in the MRI and SPECT-CT examination to determine the fracture of different periods of responsibility of the vertebral body, but the sensitivity of SPECT-CT is higher than MRI examination;SPECT-CT is an effective inspection method in the diagnosis of vertebral fractures.

Objective:To evaluate the impact of altered collagen Ⅱ(CⅡ) peptide(S268-270) on T cell activation.Methods:Altered CⅡ263-272 with G268,P269 and K270 consecutively substitution with A or G were synthesized using solid phase various concentrations were added to HLADR1 transfected APC.Expression of FITC stainning was analyzed by fluorescence-activated cell sorting,and the binding of altered CⅡ peptide to HLA-DR1 molecule on cell membrane was evaluated.Irridiated HLA-DR1 expressing APC was incubated with CⅡ263-272 or hemagglutinin(HA)306-318 and altered CⅡ peptides at various concentrations for 2 hours before T cells(3.19) were added.Supernatant was harvested and then added to IL-2 dependent CTLL cell.The cell proliferation was examined by methotetrazolium(MTT) method.Results:The altered CⅡ peptide and wild type of CⅡ263-272 were able to competitively bind to HLA-DR1 molecule expressed on cell membrane of transfected APC.CⅡ or HA induced T cell activation was significantly inhibited by the altered peptide S268-270.Conclusion:Altered CⅡ263-272 with G268,P269 and K270 consecutively substitutions with A or G inhibited CⅡ263-272 and HA306-318 induced T cell activation through competitively binding to HLA-DR1,suggesting a new approach in inhibition of T cell activation in RA.

Objective To investigate the clinical forensic assessment on cases of suspected medical malpractice and the other related aspects concerning the assessment in clinical forensic expertise. Methods Clinical data from 25 cases expertized for suspicion of medical malpractice were retrospectively analyzed. Results 16 cases were due to the medical muff (10 cases of 16 medical muff had a bad consequence and happened in the department of operation, 6 cases had not ones); 9 cases were non-medical muff. Conclusion Though the medical muff recognized is basic, but the relationship between the medical muff and the following bad results of medical muff is the best key for the forensic expertise. Otherwise, the scientific and practical value must be considered in the forensic expertise.

Objective To study the function of the laparoscope in abdominal trauma diagnosis and treatment.Method To use laparoscope in diagnosis and treatment for 20 cases abdominal trauma.Result 11 cases avoided the operation of the open abdomin,7 cases got treatment under the laparoscope;5 cases avoided the negative exploration.Conclusion Laparoscope can be used to clear the diagnosis.avoid negative exploration,treat the injury and help operator to choose incision and type of operation.

Objective To study an effective pathway in preventing the recurrence of hepatocellular carcinoma(H.C.C) and improve the long-term curative effects.Methods 103 resectable cases of H.C.C were randomly divided into treatment group,hepatectomy plus hepatoarteria-and portal chemotherapeutics embalizations with subcutaneous pump(HACE and PVCE) group and control group(Only hepatectomy).Based on changes of ?-fetoprotein(AFP) for pre-and post operation and results of"B" ultrasound and CT,hepatic artery portography,the difference of 1,3,5-year recurrence rates and survival rates between the two groups were compared.Results ⑴The recurrence rates of treatment group and control group for 1,3,5-year were 13.5%,46.2%,67.3% and 19.6%,60.8%,86.2% respectively,there was an obviously difference (P