Objective To detect the expression of YB-1 and to research the relationship in the occurrence and development in the cervical squamous carcinoma(SCC) tissues.Methods Immunohistochemistry(IHC) envision was used to detect the expression of chronic cervicitis,CIN Ⅰ,CIN Ⅱ-Ⅲ and SCC.The relationship of YB-1 in the clinical pathological parameters of SCC were analyzed.Results YB-1 was mainly located in the nucleus in squamous cell,sometimes in the cytoplasm.The YB-1 protein did not expression in chronic cervicitis.In CIN Ⅰ,CIN Ⅱ-lⅢ and SCC,the positive expression had a gradual increasing trend.The expression of YB-1 was satistically significant in four groups (P＜0.05).The chronic cervicitis group,CIN Ⅰ groupCIN Ⅱ-Ⅲ group compared with the SCC group restivelly,the difference was statistically significant (P＜0.05).From spearman rank correlation analysis:the expression of YB-1 was positively correlated with the degree of cervical lesions (P＜ 0.05).In the cervical squamous carcinoma group,the expression of YB-1 was not associated with clinical pathological index of SCC patients(P＞0.05).Conclusion The change of the quantity of YB-1 protein is closely related to cervical squamous cell carcinoma.

Objective To investigate the therapeutic effect of hemofiltration (HF) on severe acute pancreatitis (SAP). Methods The data of 60 patients with SAP was retrospectively reviewed in terms of life vital signs, biochemical indexes and prognosis within 96 h. 30 cases (the average APACHE Ⅱ score was 16.64 ±4.21 ) underwent HF on the basis of traditional treatment while another 30 cases ( the average APACHE Ⅱ score was 15.05 ± 3.78) were treated traditionally. Results From the beginning to 96 h after treatment, vital signs and the biochemical indexes such as body temperature, respiration rate, heart rate, blood electrolytes, blood pressure, liver function and renal function were improved more obviously in patients who received HF treatment than those who only received traditional treatment. The hospitalization time was positively correlated with the disease onset time. Patients receiving HF treatment need shorter hospitalization time than patients receiving conventional treatment. There was no significant difference in terms of mortality rate between HF treatment group and conventional treatment group ( 16. 7% and 30% respectively). Conclusions HF treatment can improve some biochemical indexes and symptoms in SAP patients. For example, body temperature, respiration rate, heart rate,blood electrolytes, liver function, kidney function, lung function, abdominal pain and abdominal distension. It can shorten hospitalization time. However, HF has no significant effect on mortality rate.

Objective To analyse the effect of berberine on D-dimer, fibrinogen (FIB) and interleukin-6(IL-6) in peripheral blood of ovarian cancer patients.Methods 130 cases selected from the hospital were diagnosed as unilateral ovarian cancer, treated by surgery and chemotherapy.The patients were divided into control group (25 cases) and experimental group (105 cases), and the experimental group were randomly divided into A, B, C three groups, 35 cases in each group.The experimental group received berberine hydrochloride tablets, orally, three times a day, on the basis of conventional chemotherapy.The experimental groups were divided into three subgroups according to the dosage: A group: 0.3 g, B group: 0.2 g, C group:0.1 g, 35 cases in each group, continuously treated for 2 weeks.The levels of D-dimer, FIB and IL-6 and quality of life score were detected before and after treatment.Results After treatment, D-dimer level in each subgroup of experimental group was lower than that in control group, respectively, and A group