BACKGROUND: The neurological basis for learning and memory is the high plasticity of central nervous system, and special rehabilitative training is necessary in the process of the functional recombination of central nervous system.OBJECTIVE: To observe the capability of rehabilitative training in differentiating learning ability and memory maintaining of one-trial passive avoidance response, and its correlation with the kinetic properties of Nmethyl-D-aspartic acid (NMDA) receptor channel of contralateral hippocampal neuron in rats with cerebral infarction.DESIGN: A randomized control animal trial.SETTING: Department of Rehabilitative Medicine, Affiliated Hospital of Luzhou Medical College.MATERIALS: The experiment was carried out in the central laboratory of Luzhou Medical College between March 2000 and February 2002. Twentyfour male Wistar rats were randomly divided into three groups with 8 rats in each: free movement after cerebral infarction group (model group), rehabilitative training after cerebral infarction group (rehabilitation group) and normal group.METHODS: ① Model establishment: Rats in the model group and rehabilitation group were made into models of infarction of right middle cerebral artery, and those in the normal group were not given any treatment. ②Rehabilitative training: After 4 days, only the rats in the rehabilitation group were trained with rolling-cage exerciser, screen exerciser and balance exerciser for 4 weeks. ③ Learning and memory tests: The learning and memory performances of all the rats were tested at 35 days postoperatively. The Y-maze test was applied to mainly observe the needed number f training for the rats to reach 9/10 correct response (for running to dark arm), and the multi-functional conditioned reflex box to observe the stayed duration on the diving board (step-through latency). ④ The single channel current property of NMDA receptor in hippocampal neurons was recorded with cell adhesion after learning and memory tests.MAIN OUTCOME MEASURES: ① training times needed for the rats to grasp the structure of the maze in each group; ② step-through latency in each group; ③ single channel current property of NMDA receptor in each group.RESULTS: All the 24 rats were involved in the analysis of results. ① The training times needed for the rats to grasp the structure of the maze in the rehabilitation group and normal group were significantly different from that in the model group [(68.02±11.67), (57.62±10.31), (107.07±16.32) times, P ＜0.05], and there was no significant difference between the rehabilitation group and normal group (P＞0.05). ② The medians of step-through latency before shock in the rehabilitation group and normal group were significantly different from that in the model group (286.7 s, 298.4 s, 126.7 s, P ＞0.05), and there was no significant difference between the rehabilitation group and normal group (P＞0.05). ③ The 35 pS short open NMDA receptor channel mainly existed in the hippocampal neurons of rats in the rehabilitation group, the open proportion was 0.099±0.007. The duration and proportion of 20 pS short and long open and the 35 pS short open NMDA receptor channels in the hippocampal neurons of rats were not significantly different from those in the normal group (P＞0.05). In the model group, 20 pS open channel was mainly observed, and the durations of 20 pS short and long open channels were obviously shorter than those in the rehabilitation group, the open proportion of 35 pS short open channel was 0.036t0.04, which was lower than that in the rehabilitation group,but no 35 pS long open channel was observed.CONCLUSION: Rehabilitative training accelerates the recovery of the learning and memory abilities of rats with cerebral infarction by changing the NMDA receptor channel properties of contralateral hippocampal neurons.

OBJECTIVE:To explore the effect of health intervention on blood pressure,medication compliance and the quali-ty of life in elderly patients with hypertension. METHODS:64 elderly patients with hypertension were selected and randomly divid-ed into observation group and control group,with 32 cases in each group. Both groups were given Amlodipine besylate tablet 5 mg,qd. Control group received routine hypertension knowledge education and regular recheck by guidance. Observation group addi-tionally received health intervention on the basis of control group. The blood pressure control,cognitive level of patients to hyper-tension,medication compliance,the level of trust in physicians and quality score of life were compared between 2 groups before and after intervention. RESULTS:There was no statistical significance in systolic blood pressure and diastolic blood pressure be-tween 2 groups before intervention (P>0.05). After intervention,systolic blood pressure and diastolic blood pressure of 2 groups were decreased significantly,and the observation group was significantly lower than the control group,with statistical significance (P<0.05). There was no statistical significance in cognitive level of patients to hypertension,medication compliance,the level of trust in physicians and quality score of life between 2 groups before intervention (P>0.05). After intervention,the observation group was better than the control group in terms of cognitive level of patients to hypertension,medication compliance,the level of trust in physicians and quality score of life,with statistical significance(P<0.05). CONCLUSIONS:Health intervention can effec-tively improve the cognitive level of elderly patients with hypertension to hypertension,improve the medication compliance and the level of trust in physicians,control blood pressure and improve the quality of life.

Objective To observe the effect of neuropeptide Y(NPY) on the levels of serum interleukins(ILs)in guinea pigs with experimental allergic encephalomyelitis(EAE).Methods 30 guinea pigs were divided randomly into normol control group,EAE group and NPY group.The NPY was injected into the intracerebroventricular in NPY group one week before the EAE model be made.The EAE molders were made by injecting the homogenate of rat's spinal cord into the plantar of guinea pigs in groups EAE and NPY.Then,the neurological deficits score was observed everyday,the incidence and the delitescence of EAE were observed too.The levels of serum IL-1?,IL-2,IL-4,IL-6 and IL-8 were detected,and the neuropathological examination was used when symotoms peak period of EAE models.Results(1)The incidences of the EAE in groups EAE and NPY were 100% and 90%,the delitescences of EAE were(10.0?4.8)d and(25.4?12.6)d,the neurological deficits scores at the symotoms peak period were(3.60?0.52) and(1.80?1.14),respectively.There were significantly differences between the two groups(all P

Objective To explore the effect of BCG-polysaccharide nucleic acid(BCG-PSN) on the numbers of Th3,Th2 and Th1 cells in peripheral blood mononuclear cell(PBMC) and airway inflammation status in patients with asthma.Methods Twenty-six patients with moderate persistent asthma were enrolled into the study and randomly divided into simple medication group(n=13,accepted Fluticasone/salmeterol alone) and combined medication group(n=13,accepted Fluticasone/salmeterol+BCG-PSN together).The numbers of Th3,Th1 and Th2 cells in PBMC, the serum levels of TGF-β,IFN-γ,IL-4 and IgE and the clinical effect and airway inflammation status were observed at three time points: before,1.5 months after and 3 months after treatment. Results (1)At 1.5 months after treatment,the Th1/Th2 number was significantly higher in the combined medication group than the simple medication group(1.20±0.57 vs 0.79±0.39,t= 2.129,P＜0.05),and the asthma control test scale(ACT) showed similar difference(18.31±1.75 vs.15.54±2.40,t=3.359,P＜0.01) between the two groups.(2)At 3 months after treatment,the Th1/Th2 number was significantly higher in the combined medication group than the simple medication group(1.73±0.74 vs1.16±0.48,t=2.327,P＜0.05),and the ACT also showed the same kind of difference(22.46±1.13 vs.20.23±2.59,t=2.851,P＜0.01) between the two groups.In addition,at this time point the PEF variability was significant lower in the combined medication group than the simple medication group([9.88±2.18]% vs.[12.05±2.74]%,t=2.235,P＜0.05).(3)We found no significant differences in the comparisons of the numbers of T helper cell subsets in PBMC ,the serum levels of cytokines and IgE,the numbers of inflammatory cells in induced sputum between the two groups(Ps＞0.05).(4)No correlation were found between Th3 cell numbers in PBMC and the serum levels of IgE in the two groups.Conclusion The combined use of Fluticasone/salmeterol and BCG-PSN can further correct the imbalance of Th1/Th2 cells,alleviate clinical symptom of asthma and airway hyper-responsiveness.The therapeutic efficacy improves along with the therapy period,better in the combined medication group than the simple medication group.BCG-PSN has no significant effects on the numbers of Th3 in PBMC in patients with asthma.

Objective To explore the expression of GATA-3 in pulmonary tissue of asthmatic mice and the inhibitory effect of dexamethsone(Dex)on it.Methods The Blab/c mice asthma model was induced by ovalbumin(OVA) with classic method.Twenty-four male mice were randomly divided into control group,asthmatic group and Dex treated group.The expression of GATA-3 protein was measured by immunohistochemical staining.The expression of GATA-3 mRNA was measured by reverse transcription-polymerase chain reaction(RT-PCR).The level of IL-4 in mice spleen CD4 T cell was measured by flow cytometry.The airway inflammation was evaluated by HE staining.Results The percentages of postive GATA-3,GATA-3 mRNA and IL-4 protein of asthmatic group were significantly higher than those of control group(P

The 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase activity of Sclerotinia sclerotiorum is one of the multifunctional enzyme AROM activities, which catalyzes a reversible conversion of shikimate 3-phosphate (S3P) and phosphoenolpyruvate (PEP) to EPSP and inorganic phosphate, and is inhibited by the herbicide glyphosate (N-phosphonomethyl glycine). AROM protein has been purified from Sclerotinia sclerotiorum and the EPSP synthase has been analyzed. The results indicated that the optimal pH and temperature of EPSP synthase were 7.2 and 30℃ respectively. The activation energy of the heat-deactivated reaction of the enzyme was found to be 69.62 kJ/mol. Both of the substrates, S3P and PEP, were showed to inhibit the reaction rate when their concentrations exceeded 1 mmol/L and 2 mmol/L respectively. The Km of 140.98 μmol/L for PEP and 139.58 μmol/L for S3P were obtained by Dalziel equation which was a steadystate kinetic equation of the enzymatic reaction with the double substrates. The kinetic pattern of the enzyme was consistent with a sequential mechanism. Inhibition of the EPSP synthase reaction by glyphosate was competitive with respect to PEP, with the Ki 0. 32 μmol/L, and noncompetitive with regard to S3P. Activation by [ K+ ] was observed in the forward reaction. The Km (PEP) was lowered by increasing [ K+ ], while the Km (S3P) changed irregularly and the Ki (PEP) was enhanced.

Objective To explore the relationship between different airway pressure and expression of related cytokines to airway remodeling.Methods Fourty-two chronic obstructive pulmonary disease cases (COPD group) and thirty-three control cases were collected.The above cases underwent mechanical ventilation in the period of general anesthesia.According to different levels of peak inspiratory pressure(PIP), above two groups, randomly and respectively, were divided into high PIP (HPIP, 24 cmH2O) group, moderate PIP (MPIP, 22 cmH2O) group, low PIP (LPIP, 20 cmH2O) group.All positive end expiratory pressure (PEEP) was set to 5 cmH2O.Then the collection of BALF was performed before and 3 hours after applying ventilator.The related factors to airway remodeling, such as fibroblast growth factor 2 (FGF-2), transforming growth factor β1 (TGF-β1) and matrix metalloproteinase-9 (MMP-9), were measured by enzyme-linked immunosorbent assay (ELISA) and Western blot.Results 1)Beforemechanical ventilation, the levels of FGF-2, TGF-β1 and MMP-9 in COPD group were higher than control group (P<0.01).2)3 hours after mechanical ventilation, we saw significant upregulated expression of FGF-2, TGF-β1, MMP-9 in HPIP subgroup in control group (P<0.05) and the 3 factors levels of COPD group were all increased (P<0.05).Moreover, HPIP subgroup was significantly higher than MPIP and LPIP subgroup in COPD group (P<0.05).3)The expression of FGF-2, TGF-β1, MMP-9 in BALF had a positive correlation with the airway pressure levels in COPD group(P<0.01).Conclusions Under mechanical ventilation, sustained high airway pressure may enhance the expression of FGF-2, TGF-β1 and MMP-9 which may result in airway remodeling by mechanosensitive cation channel in bronchial epithelial cells, especially in COPD patients.

Objective To compare the effects of classic intrafascial supracervical hysterectomy(CISH)and laparoscope-assisted vaginal hysterectomy(LAVH)on structure and function of the pelvic floor in women.Methods From March 2003 to December 2005,totally 35 cases of CISH and 44 cases of LAVH were performed in our hospital.The patients were followed up for 22 to 38 months after the operations.During the period,they received physical examination and life quality questionnaire,by which the degree of pelvic organ prolapse and pelvic floor function were evaluated.Results The incidence of cystocele in CISH group was significantly lower than that in LAVH group [11.4%(4/35)vs 34.1%(15/44),?2=5.481,P=0.019],whereas,no significant difference was found in the incidence of rectocele between the two groups [17.1%(6/35)vs 13.6%(6/44),?2=0.186,P=0.666].Seven(15.9%)patients in the LAVH group developed cervix prolapse,while none of the CISH group had such a situation(P=0.016).Life quality questionnaire showed that 5 patients in CISH group and 16 patients in LAVH group were satisfied with their sexual life [14.3%(5/35)vs 38.1%(16/42),?2=5.456,P=0.019].Conclusion CISH is superior to LAVH in protecting the structure and function of the pelvic floor.

We achieved satisfactory results in preventing post-colostomy cutaneous complications by targeted nursing interference.This article summarizes the experience in nursing three colorectal carcinoma patients against such complications after colostomy,with an analysis of the common causes of stomy-related cutaneous complications.

AIM:To observe the influence of high expression of miR-15a-5p on the proliferation and migration of human hepatocellular carcinoma SMMC-7721 cells.METHODS: The miR-15a-5p oligonucleotide , which was recon-structed with additional restriction sites of EcoRⅠand HindⅢ, was chemically synthesized and confirmed by sequencing . The miR-15a-5p eukaryotic expression system was constructed by pcDNA 6.2-GW/Em-GFP-pre-miR-15a-5p plasmid.The miR-15a-5p was transfected into the SMMC-7721 cells transiently by plasmid , and quantified by quantitative real-time PCR at the mRNA level.The cell viability was measured by CCK-8 assay, and the living cell counting was performed by the method of Trypan blue exclusion .The migration ability of the SMMC-7721 cells with high expression of miR-15a-5p was de-tected by wound healing test .RESULTS: The sequence of miR-15a-5p oligonucleotide 100% matched the designed se-quence.Compared with control group , the miR-15a-5p expression was increased significantly (P<0.05).The viability, the living cell number and the migration ability of the SMMC-7721 cells were decreased in high expression of miR-15a-5p group with statistically significant difference (P<0.05).CONCLUSION: The abilities of proliferation and migration in human hepatocellular carcinoma SMMC-7721 cells are decreased by high expression of miR-15a-5p.