It is important to analyze the epidermal growth factor receptor ( EGFR) mutation before makingstrategyonnon-small cell lung cancer ( NSCLC) patients scheduled to EGFR tyrosine kinase inhibitor ( TKI) therapy .Digital PCR is a new generation of molecular diagnostic technique that provides ultra-highersensitive, specific and absolute nucleic acid quantification based on its unique principle.The application of digital PCR indetecting circulate tumor DNA can be the truly tumorliquid biopsy, helps to acquire the accurate EGFR mutation status from peripheral blood and screen out the most appropriate patients for TKI therapy.This breakthrough technology will also contribute to tumor surveillance and drug resistance monitoring.

BACKGROUND: Physical therapy is an effective treatment for movement disorder caused by central nerve system injury, while incorrect rehabilitative method will exacerbate the movement disorders in patients.OBJECTIVE: To investigate the effect of physical therapy on movement and daily activity of the patients suffering from brain injury.DESIGN: Randomized controlled study based on patients with a confirmativediagnosis.SETTING: Rehabilitation department in a university hospital.PARTICIPANTS: From September 2001 to August 2002, 78 patients hospitalized in the Rehabilitation Department of the Hospital Affiliated to Luzhou Medical College, with hemiplegia caused by brain injury, were selected into this study. The patients with severe brain injury, severe understanding disability, and severe heart diseases, lung diseases and kidney diseases were excluded.METHODS: The eligible patients were divided into two groups: the physical therapy group(48 patients) and the control group(30 patients) . All the patients in these two groups received medication and the hyperbaric oxygen therapy (HBOT), while the patients in the physical therapy group received an extra physical therapy.MAIN OUTCOMES MEASURES: A Fugl-Meyer assessment(FMA) and a modified barthel index(MBI) were adopted to evaluate the pre-and post-treatment statuses of the patients in these two groups.RESULTS: No significant difference in age, sex and disease course between the physical therapy group and the control group was found. In the physical therapy group, the FMA scale before and after the therapy were 40.43 ± 21.78 and 68.35 ±23.39, and the corresponding MBI scale were 32.82 ± 17.40 and 78.84 ± 25.31 respectively. In the control group,meanwhile, the FMA scales were 41.71 ± 19. 13 and 51.48 ± 22.58, and the MBI scales were 33.02 ± 12.48 and 56.65 ± 26.53. Before the therapy,comparison of FMA and MBI between the two groups showed no significant difference. While after the therapy, significant difference of FMA and MBI between the two groups could be confirmed( t = 2. 14, 2.21, P ＜ 0.05).CONCLUSION: Physical therapy could apparently enhance the rehabilitation of movement ability in patients with brain injury.

Objective To analyse the clinical usage of Shenmai injection, promote the rational use of Shenmai injection. Methods 400 hospital medical records that used Shenmai injection in 2015 were collected and reviewed from hospital information system (HIS) by retrospective analysis method, aggregated and evaluated data by EXCEL. Results In 400 cases, 11 clinical departments used the Shenmai injection, mainly in cardiology of 347 cases(86.75%).The percentage of the non-standard clinical application of Shenmai injection was 84 cases (21.00%). The non-standardized rates of solvent dispensing was 287 cases (71.75%), and the specification incompatibility accounted for 105 cases (26.25%). There was no adverse drug reaction of Shenmai injection. Conclusion The clinical use of Shenmai injection is not yet standardized, which suggests that drugs should be strictly used in accordance with the instruction, in order to avoid risks of off-label use.

Objective To explore the expression of GATA-3 in pulmonary tissue of asthmatic mice and the inhibitory effect of dexamethsone(Dex)on it.Methods The Blab/c mice asthma model was induced by ovalbumin(OVA) with classic method.Twenty-four male mice were randomly divided into control group,asthmatic group and Dex treated group.The expression of GATA-3 protein was measured by immunohistochemical staining.The expression of GATA-3 mRNA was measured by reverse transcription-polymerase chain reaction(RT-PCR).The level of IL-4 in mice spleen CD4 T cell was measured by flow cytometry.The airway inflammation was evaluated by HE staining.Results The percentages of postive GATA-3,GATA-3 mRNA and IL-4 protein of asthmatic group were significantly higher than those of control group(P

Objective To investigate the role of CD4+CD25+FoxP3+ in severe Mycoplasma pneumonia among children.Methods One hundred and forty children with M.pneumoniae pneumonia (65 severe and 75 non-severe) who were hospitalized were enrolled along with forty other children as controls.X-ray was assessed.The proportions of peripheral blood CD4+CD25+FoxP3+cells were determined by flow cytometry.Results Both severe and non-severe children had decreased CD4+CD25+FoxP3+cells as compared with control subjects in acute phase (0.87 ± 0.66％ vs.3.88 ± 2.00％,P ＜ 0.01 and 1.17 ± 0.70％ vs.3.88 ±2.00％,P ＜0.01,respectively).The levels of CD4+CD25+FoxP3+cells in severe children were lower than those in non-severe children in acute phase and recovery phase (0.87 ±0.66％ vs.1.17 ±0.70％,P ＜0.05 and 1.66 ±0.85％ vs.3.61 ± 1.45％,P＜0.01,respectively).Both severe children and non-severe children expressed higher CD4+CD25+FoxP3+cells in recovery phase than in acute phase (1.66 ± 0.85 ％ vs.0.87 ± 0.66％,P ＜0.01 and 3.61 ± 1.45％ vs.1.17 ±0.70％,P ＜0.01,respectively).Conclusion The expression of CD4+CD25+FoxP3+Tregs may play a role in the onset of severity of mycoplasma pneumonia and the low express of CD4+CD25+FoxP3+Tregs in children infected with M.pneumonia may increase the susceptibility to severe mycoplasma pneumonia.

Objective Published literatures on the relationship between IL-13 gene polymorphism and the susceptibility of children to bronchial asthma in China were comprehensively analyzed with the use of Meta-analysis to evaluate this relationship.Methods The data were collected from the Medline database,Ovid database,the Cochrane library,and Chinese Biomedical database,and the references of eligible studies were manually screened.Published data related to case-control studies reporting the link between IL-13 polymorphisms and asthma in Chinese children were retrieved through those database.Meta-analysis was conducted to determine whether the IL-13 gene polymorphisms were associated with asthma.Results Eighteen studies were finally accepted for analysis.There were three studies focusing on C-1 112T polymorphism,and six studies focusing on C + 1923T polymorphism,and fourteen studies focusing on G + 2044A polymorphism.There was no evidence to confirm that the genotypes in position IL-13-1112 C/T were associated with asthma in Chinese children [odds ratio(OR) =1.00,95％ CI 0.82-1.22,P =0.98].The OR of asthma for TT/CC genotypes was 1.15 (95 ％ CI 0.57-2.33,P =0.69) and for CT/CC was 1.01 (95 ％ CI 0.82-1.25,P =0.89).There was significant evidence to confirm that the genotypes in position + 1923 C/T were associated with asthma in Chinese children(OR =1.86,95％ CI 1.29-2.67,P =0.000 9).The OR of asthma for TT/CC genotypes was 2.12 (95 ％ CI 1.27-3.56,P =0.004) and for TC/CC was 1.67 (95％ CI 1.18-2.35,P =0.003).There was no correlation between IL-13 + 2044G/A polymorphism and the susceptibility (OR =1.33,95％ CI 0.94-1.88,P =0.11).The OR of asthma for AA/GG genotypes was 1.30 (95 ％ CI 0.76-2.20,P =0.34) and for AG/GG was 1.24(95％ CI 0.90-1.70,P =0.19).Conclusions IL-13 gene + 1923 TT and TC genotypes should be associated with susceptibility of asthma in Chinese children,and the T allele could increase the risk of asthma.No clear relationship was found between the genotype TT/TC at the IL-13-1112 site and the incidence of asthma of children in China,and so was the genotype AA/AG at the IL-13 +2044 site and the incidence.

Objective To assess the in vitro affinity and apoptosis-inducing effect of 131I-K237 peptide (H-His-Thr-Met-Tyr-Tyr-His-His-Tyr-Gln-His-His-Leu-OH) to LNCaP prostate cancer cell line.Methods The K237 peptide was radiolabeled with 131I by the Iodogen method.The radiolabeling efficiency and radiochemical purity after purification were then characterized by TLC in vitro.LNCaP cells were inoculated in 96-well cell plate and divided into following groups (3 duplicate wells for each group):15 kBq 131I-K237was added in the experimental group,different doses of Na131I (5,10,15 kBq) were added in 3 negative control groups,15 kBq 131I-K237 with different doses of unlabeled K237 (1,2,4,8,16 μ g/μl) were added in 3 blocking groups,and PBS was added in blank control group.The cellular binding ratios were calculated after 48 h.LNCaP cells were inoculated in 24-well cell plate and divided into 3 groups:131I-K237group,which including 3 different dose subgroups (5,10,15 kBq) ; unlabeled K237 group,which including 3 different dose subgroups (1,2,4 μg/μl) ; blank control group with 100 μl PBS.All the cells were cultured for 48 h,then optical microscopy (OM) and fluorescence microscopy (FM) were used to observe the cell morphology ; DNA gel electrophoresis was conducted and flow cytometry (FCM) was used to estimate the apoptotic rate of LNCaP cells.One-way analysis of variance and the least significant difference (LSD)-t test were used to analyze the data.Results The labeling efficiency of 131I-K237 was (73.7±3.2) ％ and the radiochemical purity was (96.7±0.6) ％ after purification.The binding ratio of experimental group was (95.8±1.5)％,whereas the ratio of negative groups with 5,10,15 kBq Na131I and PBS group was (8.2±0.4) ％,(8.3±0.6) ％,(8.6±0.5) ％ and 0,respectively.The binding ratio of 131I-K237 and LNCaP significantly declined with the increased dose of unlabeled K237 (t=4.71,P＜0.01).The apoptosis of LNCaP cells cultured with 131I-K237 was observed.Typical DNA ladder was found by DNA gel electrophoresis.The apoptotic rates of 5,10,15 kBq131I-K237 groups were (34.1±2.9)％,(37.3±3.4)％ and (41.7±3.6)％,respectively; whereas those of unlabeled K237 groups and blank control group were (10.8±1.0) ％,(12.5±2.1) ％,(13.1±2.4) ％ and (2.9±0.3) ％,respectively.There were significant differences of apoptotic rate among groups (F=76.31,P＜0.05).The difference among 5,10,15 kBq 131I-K237 groups was statistically significant (t=3.09,3.27,4.52,all P＜0.05).Conclusion 131I-K237 can bind to LNCaP cells with highly affinity and has significant apoptosis-inducing efficacy on the prostate cancer cell line.

Objective To compare the effect of intracoronary versus intravenous administration of tiroifban for acute coronary syndrome (ACS) patients during percutaneous coronary intervention (PCI). Methods A search was retrieved from Pubmed, EMbase, Chinese Biomedical Literature Database (CBM), Chinese Journal Full-text Database (CNKI), Chinese Science and Technology Periodical Database (VIP), Cochrane Library to systematically collect the randomized controlled trials of intracoronary versus intravenous administration of tirofiban for the patients with ACS undergoing PCI. The data was extracted from the included studies and analyzed by Cochrane Collaboration's RevMan5.2 software. Results Twenty-five studies involving 2516 patients met the inclusion criteria. The results of meta-analysis showed that thrombolysis in myocardial infarction (TIMI) grade 3 lfow (RR 1.15, 95%CI 1.07-1.23, P=0.0001) were signiifcantly more often achieved in the patients by intracoronary administration of tiroifban (IC group) than those by intravenous strategy (IV group). Left ventricular ejection fraction (LVEF) values in a week after PCI which were evaluated by Cardiac Ultrasound were statistically significant between the two groups (WMD 2.69, 95%CI 0.14-5.25, P=0.04). LVEF values in IC group were increased by an average of 2.69% compared with group IV. Intracoronary administration resulted in a reduced incidence of major adverse cardiovascular events (MACE) at 30-day follow-up (RR 0.51, 95%CI 0.38-0.69, P < 0.0001). However, the incidence of bleeding complications was not statistically signiifcant between the two groups (RR 0.95, 95% CI 0.76-1.19, P=0.64). Conclusions Compared with intravenous strategy, intracoronary administration of tiroifban can be more effective in increasing coronary blood lfow and microvascular perfusion, more signiifcantly in reducing the incidence of MACE at 30-day follow-up and improving the prognosis after PCI without increasing the risk of bleeding.

Objective To study the application effect of feedback training mode on new neonatal recovery technology.Methods 101 midwives,obstetric doctors and pediatricians who participated the training were tested about the professional knowledge and skills of new neonatal recovery technology according to the teaching material of neonatal asphyxia recovery and neonatal resuscitation guidelines (2007 revision in Beijing).The results showed no statistical significance.And they were randomly divided into the control group (51 cases) and the observation group(50 cases).The control group adopted traditional method,that was,the teacher taught first and then the students took practice.The observation group used feedback training method.Results The theoretical and technical results of the observation group were significantly higher than those of the control group.The learning motivation,learning initiative,cooperation degree,ability of observation and solving problem and operation proficiency were also significantly higher than those of the control group.Conclusions Feedback training methods can significantly increase the learning effect of perinatal personnel,and make popularized the new neonatal recovery technology.

Adolescent ; Adult ; Burns, Chemical ; Eye Burns ; Fisheries ; Humans ; Hydrogen Sulfide ; Middle Aged ; Occupational Injuries ; Young Adult