Objective:To observe the role and value of advanced venipuncture virtual training system in nursing clinical skills training for different venipuncture cases.Methods:Forty pre-service nurses were randomly selected from clinical departments to carry out training, and 40 trainees were respectively tested on the theory and operation of virtual venipuncture technology before and after training by the system software. A self-control study was conducted before and after training (random case selection assessment). Then a questionnaire was used to evaluate the training effect. SPSS 19.0 was performed for t test and repeated measure-ment ANOVA analysis. Results:Forty nurses participated in venipuncture training and passed the examination smoothly. The results of theoretical and operational assessment before and after training show significant differences.Conclusion:After training the pre-service nurses with the advanced virtual venipuncture system, the flexibility and accuracy of the operation are significantly improved, and the venipuncture operation can be accomplished skillfully and quickly.

Humans ; Interleukin-18 ; chemistry ; metabolism ; Pulmonary Fibrosis ; etiology ; pathology ; Silicosis ; complications ; pathology ; physiopathology

Female ; Gestational Age ; Globins ; Humans ; Infant, Newborn ; Infant, Premature ; Male ; Nerve Tissue Proteins ; blood

Objective To evaluate the clinical significance of 18F-FDG PET/CT in localization and etiologic diagnosis of suspected pacemaker-related infection(SPRI).Methods Fifteen SPRI patients (10males,5 females,age range:45-86 years) were included in this retrospectively study.18F-FDG uptake in device-related region and abnormal 18F-FDG uptake out of the device region were visually interpreted.Final diagnosis was decided according to modified Duke's criteria and imaging interpretation.Diagnostic efficiency of 18 F-FDG PET/CT on SPRI was evaluated.Results Of 7 patients with suspected pocket infection,5 patients had hot spot of 18F-FDG in the pocket site,either in superficial skin and/or deep soft tissue.Of 9 patients with suspected infective endocarditis,a diffuse FDG uptake was demonstrated in 3 patients in the area of left atrium,right atrium,and right ventricle;another 3 patients had abnormal FDG uptake in other sites rather than in the implantation regions,and the rest 3 showed negative PET/CT results.In patients with SPRI,18F-FDG PET/CT diagnosed 7 cases of pocket infection,8 cases of infective endocarditis,14 cases of pacemaker-related infection.Conclusions 18F-FDG PET/CT imaging is useful in localization and etiologic diagnosis in SPRI patients.It might guide the clinical SPRI management.

Bone marrow mesenchymal cells (BMSCs) are regarded as donor cells in cell transplantation therapies for spinal cord injury (SCI) for they have the ability of favourable proliferation and multi-directional differentiation, and are easily isolated and culturd and have less immunological reaction. It has been confirmed that subarachnoid space injection is the most ideal delivery technique of BMSCs. Bone marrow mesenchymal stem cells transplantation is safe and its reconditioning role is certain for SCI in early clinical application. The mechanism of BMSCs promoting functional recovery after SCI is probably concerned with vicarious function, nerve trophism, immunosuppression and promoting axonal regeneration by BMSCs.
Animals ; Bone Marrow Cells ; cytology ; Humans ; Mesenchymal Stem Cell Transplantation ; methods ; Mesenchymal Stromal Cells ; cytology ; Spinal Cord Injuries ; surgery

Objective Probe application value of CHN(the standards of skeletal maturity of hand and wrist for Chinese-CHN method)bone age in judicial identification,and analyze factors and related problems which influence its accuracy.Methods 522 patients participated in judicial identification of living age estimation.Two posteroanterior radiographs for the left wrist were shot with respective exposure value for all patients.And two oblique radiographs were added.Bone ages were estimated according to CHN method.Result Among 522 cases were 489 males and 33 females.Bone ages of 129 males reached to tiptop of CHN-18.4 and average bone age of other male patients is 16.5.Bone ages of 14 females reached to tiptop of CHN-17.3 and average bone age of other female patients is 15.7.Among 522 cases there were 388 patients with their CHN bone ages above 16.0,about 74.3%.The group of data indicates that multiple factors can influence accuracy of bone age estimation.Among them change of radiograph position and angle influenced estimation accuracy most.Conclusion If radiographs of high quality can be acquired,CHN method can be proficiently used,and radiologists can pay attention to the effect on estimation accuracy of epiphysis classification by change of radiograph position and angle,then CHN bone age can mostly meet requirements of living age estimation.

Objective To observe the influence of dexamethasone on M1 BMDM and alveolar macrophage differentiation in respiratory syncytial virus ( RSV) infected mice.Methods Culture M1 BMDM and identify its phenotype, observe the effect of DEX on its transcription factors and cytokines; In RSV infected mice, observe the influence of DEX on the transcription factors and cytokines of alveolar macrophage.Results DEX can inhibit IL-6, IL-12, iNOS and IRF5 expression, but promote IL-10, Arginase-1 and IRF4 expression in M1 BMDM and alveolar macrophages of RSV infected mice, made M1 macrophages transform to M2 macrophages.Conclusion DEX can transform M1 macrophages to M2 macrophages in vivo and in vitro, and inhibit inflammation, this may be one of the mechanism of DEX in the treatment of RSV infection.

Objective:To investigate the effects of comprehensive intervention on the quality of life of middle school students. Methods:Eight classes were extracted with striated cluster sampling method from the first grade of two key junior middle schools in city and countryside; four classes (two classes of the urban and rural) were selected randomly as the experimental group and another four classes (two classes of the urban and rural) as the control group. The experimental group received mental-social intervention for three years. No intervention for the control group. The effect was evaluated with the subjective quality of life of children, and a questionnaire on mental health of middle school students. Results:The scores of life quality, cognitive component and emotion component of experimental group were higher than that of control:(172.6?12.8/145.4?17.4,98.7?10.8/85.0?13.3,73.9?4.3/60.4?7.7; t=20.83, 13.20, 25.29 respectively;the average P

OBJECTIVE:To prepare the curcumin solid lipid nanoparticles. METHODS:Microemulsion method was adopted to prepare curcumin solid lipid nanoparticles (SLN) and pseudo-ternary phase diagrams was conducted to optimize the preferable three-phase microemulsion factors. The optimal conditions were determined,and then hot microemulsion was dispersed in cold wa-ter to prepare SLN. After the preliminary screening of the factors (emulsifiers,lipid materials,amount of lipids,drug-to-lipid ra-tio,cold water phase temperature and the holding temperature of microemulsion)by single-factor test,with the index of encapsula-tion efficiency,the orthogonal test was conducted to optimize the formulation,followed by the verification test. RESULTS:The best microemulsion was produced at 65 ℃ with three phases consisting of stearic acid(oil phase),polysorbate 80(emulsifier)and ethanol (co-emulsifier) and the Km ratio of 1∶4. The optimal conditions for SLN were as follows as curcumin dosage of 50 mg, stearic acid of 0.5 g,cold water phase temperature of 2 ℃,holding temperature of microemulsion of 65 ℃. The encapsulation effi-ciency was 87.73% for solid lipid nanoparticles prepared,drug-loading rate was 7.72%,particle size was(156.9±2.2)nm,poly-dispersity coefficient was 0.480,average Zeta potential was -24.8 mV(RSD<2%,n=3). CONCLUSIONS:The microemulsion method to prepare SLN is simple and feasible.

Objective To explore the inhibitory effect of astragalus polysaccharide (APS) on the proliferation of human erythroleukemia K562 cells and its mechanisms.Methods After K562 cells (purchased from Shanghai cell bank of chinese academy of science) were treated with different concentrations (0 mg/L,100 mg/L,200 mg/L and 400 mg/L) of APS.The influences of APS on the growth rate,doubling time and cell cycle distribution of K562 cells were observed by methyl thiazolyl tetra-zolium assay (MTF) and flow cytometry,respectively.Furthermore,the reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting assay were used to detect the expressions of Cyclin A,Cyclin B,Cyclin E and p21 gene at the mRNA and protein levels,respectively.Results MTT assay findings showed that,compared to the control group (0 mg/L APS),growth rates of K562 cells treated with 100 mg/L,200 mg/L and 400 mg/L APS decreased significantly (all P ＜ 0.01),and the doubling times lengthened significantly (all P ＜ 0.01).Flow cytometry findings revealed that,compared to the control group,the G1 phase cells in K562 cells of APS group increased significantly (P ＜0.01),while the S and G2/M phase cells decreased significantly (all P ＜ 0.01).RT-PCR and Western blotting results indicated that Cyclin B and Cyclin E expression of K562 cells at the mRNA and protein levels in the APS group were significantly lower than those of the control group(all P ＜ 0.01),whereas p21 expression was significantly enhanced at mRNA and protein levels (P ＜ 0.01),and Cyclin A expression was not significantly different at mRNA and protein levels between the 2 groups (all P ＞ 0.05).Conclusions APS could inhibit the proliferation of human erythroleukemia K562 cells.APS could inhibit the proliferation of K562 cells by down-regulating the expression of Cyclin B and Cyclin E and up-regulating the expression of p21.