MicroRNAs (micro-ribonucleic acid,miRNAs) are small non-coding RNA molecules,which play an important role in regulating gene transcription.Recent research has found that miRNAs can be used as a new biomarker because of being involved in the progresses of cardiomyocyte development,proliferation and apoptosis.They play an important role in the early diagnosis,prognosis and treatment of acute myocardial infarction.However,there are still many problems and challenges in detection technology,mechanism research and clinical trials of miRNAs.

Objective To explore the relationship between serum YKL-40 levels and endothelial function in patients with essential hypertension (EH).Method Sixty EH patients [34 male,aged between 43-76 years,mean(59 ±7)years]and 30 healthy subjects [17 male,mean age (57 ±5) years] were enrolled in this study.Serum YKL-40 levels were measured by enzyme immunoassay (ELISA).Endothelial function [endothelin-1 (ET-1),nitric oxide (NO),flow-mediated dilatation (FMD)] was also measured.EH patients were further divided to no metabolic syndrome and metabolic syndrome group.Results Serum uric acid,ET-1,hs-CRP were significantly higher while serum NO,FMD and NMD were significantly lower in EH group than in control group (all P ＜ 0.05).YKL-40 was significantly higher in EH group than in the control group [51.7 (35.6-341.9) μg/L vs.33.2 (23.3-167.3) μg/L,P ＜ 0.05] and significantly higher in EH patients with metabolic syndrome than in EH patients without metabolic syndrome(152.3 μg/L vs.94.2 μg/L,P ＜0.05).In this cohort,serum YKL-40 level was positively correlated with SBP,DBP,BMI,TG and hsCRP(r =0.360,0.303,0.281,0.216,0.530,all P ＜ 0.05) but not correlated with FMD,ET-1 and NO (all P ＞ 0.05).Conclusions Serum YKL-40 levels are increased compared to normal controls and positively correlated with blood pressure level but not with endothelial function parameters in hypertensive patients.Serum YKL-40 level might thus be used as a biomarker reflecting inflammation status other than endothelium function in hypertensive patients.

Objective To determine the prevalence and to identify risk factors of peri-procedure electrical storm (ES) in patients with acute myocardial infarction (AMI) underwent emergency percutaneous coronary intervention (PCI).Methods The clinical data of 228 AMI patients underwent emergency PCI were retrospectively analyzed and patients were divided into ES group (n = 39) and non-ES (n = 189) group.ES was referred to spontaneous ventricular tachycardia or ventricular fibrillation occurring twice or more within 24 h and requiring emergency treatment including anti-arrhythm medicine and/or cardioversion or defibrillation.Results ES was diagnosed in 39 out of 228 patients (17.1%) during peri-procedure stage.The incidence of ES in patients with various infarct related arteries (IRA) was as follows:55.6% with left main artery (LM),23.7% with right coronary artery (RCA),12.4% with anterior descending branch (LAD) and 0 with left circumflex artery (LCX).Older age,lager diameter of IRA,higher concentration of CK-MB and cTnT,higher incidence of reporfusion arrhythmia (RA),lower grade of TIMI after PCI and higher mortality were associated with increased risks of ES (The P value was 0.043,0.012,0.036,0.018,0.001,0.049,respectively).Gender,systolic pressure,diastolic pressure,random blood glucose level,white blood count and concentration of hs-CRP were similar between ES and non-ES patients.Logistic analysis showed that the diameter of IRA (OR 2.381,95% CI 1.127-5.028,P = 0.023),TIMI grade of IRA after PCI (OR 4.744,95% CI 1.773-12.691,P = 0.002) and RA (OR 12.680,95% CI 4.360-36.879,P =0.000)were the independent risk factors of per-procedure ES in AMI patients underwent emergency PCI.Conclusions The AMI patients with LM as IRA had the highest incidence of ES during emergency PCI and the diameter of IRA,TIMI grade of IRA after PCI and RA were independent risk factors for the development of ES during peri-PCI stage.