Child ; Child, Preschool ; Enterovirus ; isolation & purification ; Enterovirus A, Human ; isolation & purification ; Female ; Fluorescence ; Hand, Foot and Mouth Disease ; virology ; Humans ; Infant ; Male ; Polymerase Chain Reaction ; methods ; RNA, Viral ; analysis

Apply PDCA cycle theory to the progress management of the national 863 program aimed at the problem of paying more attention to program application and achievement awards,but ignoring progress management.It indicated that the quality of research has been improved.So,scientific research administrators should renew their management knowledge,to improve the ability of scientific research management.

To analyze maternal and neonatal complications among late preterm birth cases and to investigate risk factors of late preterm birth. Methods This was a retrospective analysis of 258 late preterm cases (late preterm group) born in Peking University First Hospital from January 1, 2009 to December 31, 2010. Maternal comorbidity and complications, delivery modes, and neonatal complications of these 258 late preterm infants were compared with 308 term cases (term group) during the same period. Statistical analysis was performed usingχ2 test, Fisher's exact probability test, t test and logistic regression. Results In Peking University First Hospital, late preterm births accounted for 3.9%(258/6 695) of live births and 60.1%(258/429) of preterm births. The incidence of the following maternal complications among the late preterm group was higher than that among term group(all P<0.05): severe pre-eclampsia [7.4%(19/258) vs 1.0%(3/308), χ2=15.35]; preterm rupture of membrane [42.6%(110/258) vs 15.3%(47/308), χ2=52.49];cervical insufficiency [1.9%(5/258) vs 0.0%(0/308), Fisher's exact test];placenta previa[3.5%(9/258) vs 0.6%(2/308), Fisher's exact test] and placental abruption [2.7%(7/258) vs 0.3%(1/308), Fisher's exact test]. Severe pre-eclampsia was the major risk factor leading to late preterm birth. The incidence of the following neonatal complications among the late preterm group was higher than that among term group (all P<0.05):respiratory distress syndrome (NRDS) [11.6%(30/258) vs 1.6%(5/308), χ2=24.22]; hyperbilirubinemia [64.3%(166/258) vs 39.6%(122/308),χ2=34.36];electrolyte disturbance [12.8%(33/258) vs 1.6(95/308),χ2=27.96];hypothermia [7.0%(18/258) vs 2.9%(9/308),χ2=5.08];infectious pneumonia[13.6%(35/258) vs 3.2%(10/308), χ2=20.43]; leukoencephalopathy [3.1%(8/258) vs 0.3%(1/308), χ2=5.25]; low body temperature [18.6%(48/258) vs 3.6%(11/308),χ2=33.98] and neonatal asphyxia [6.2%(16/258) vs 1.0%(3/308),χ2=11.86]. The incidence of the following neonatal complications among late preterm infants born at<35 weeks gestation was higher than that among late preterm infants born at≥35 weeks gestation (all P<0.05):NRDS [30.4%(14/46) vs 7.5%(16/212) ,χ2=19.26];hyperbilirubinemia [91.3%(42/46) vs 58.5%(124/212), χ2=17.74]; electrolyte disturbance [21.7%(10/46) vs 10.8%(23/212), χ2=4.02]; intracranial hemorrhage [8.7%(4/46) vs 1.9%(4/212),χ2=3.88];leukoencephalopathy [10.9%(5/46) vs 1.4%(3/212),χ2=8.32] and neonatal asphyxia [15.2%(7/46) vs 4.2%(9/212), χ2=6.05]. Conclusions Severe pre-eclampsia is the major risk factor leading to late preterm birth. The incidence of complications among late preterm infants is higher than that among term infants. If a pregnancy has to be terminated because of maternal disorders, the pregnancy period should be extended to 35 weeks if it permits.

Coronary Vessels ; Humans ; Microcirculation

Drug-Eluting Stents ; Humans ; Prosthesis Failure

Objective To investigate the effect of Zidan decoction containing serum on endometrial receptivity in uterine immunity environment,and then to discuss if it's effect on endometrial receptivity through its controlling the expression of cell factors which related to endometrial receptivity.Methods Mice's endometrium tissue samples and uterine Natural Killer (UNK) cells were obtained from pregnant mice on gd 4.5 days and gd 12.5 days.After endometrial glandular epithelial cells,endometrial stromal cells and UNK cells were separated through primary culture,epithelial cells and endometrial stromal cells at rate of 1 ∶ 1 a certain concentration UNK were mixed together and highly purified-cultured.Different concentrations of Zidan decoction was acted on the culture solution.FBS,black and aspirin control group were setup for contrast.The expression of LIF and VEGF which influenced by Zidan decoction in the immunity environment was assayed by Realtime-PCR.Results The expression of LIF and VEGF was significantly increased in the immunity environment of uterine (in 10％ Zidan decoction group,10％ aspirin group and 10％ blank control group:LIF was 2.10±0.20,1.98±0.14,0.90±0.05 separately,and VEGF was 3.17±0.31,2.52±0.09,0.92±0.06respectively,P＜0.05).Conclusion Zidan decoction could improve endometrial receptivity by increasing the expression of cell factor which related to endometrial receptivity with the help ofUNK paracrine.

Objective To investigate genotypes and antibiotic resistance of Escherichia coli producing plasmid-mediated AmpC ?-lactamase in Anhui province.Methods A total of 407 clinical isolates of nonrepeated Escherichia coli were collected from different cities in Anhui province.AmpC ?-lactamase producing isolates were identified by cefoxitin three-dimensional test and antibiotic susceptibility was identified by agar dilution test.Plasmid extraction,PCR amplication of corresponding group was performed, followed by sequencing.Results The positive rate of cefoxitin three-dimensional test was 8.1% (33/407), and the prevalence of plasmid-mediated AmpC ?-lactamase was 3.0% (12/407).bla_(CMY-2) gene,bla_(DHA-1) gene and bla_(ACT-2) gene were identified by PCR amplification and confirmed by sequencing in 5 strains,4 strains and 2 strains,respectively.A new CMY genotype was identified,with its sequence revealed 97% identity to the deduced amino acid sequence with previous CMY-2.This is also the first report on ACT-2 genotype in China.The susceptibility test showed that all strains were resistant to cephamycins and piperacillin,and susceptible to imipenem.Two strains of Escherichia coli producing DHA-1 were resistant to fourth-generation cephalosporin.Conclusions CMY-2,DHA-1 and ACT-2 are the most common genotypes in plasmid-mediated AmpC ?-lactamase produced by clinical Escherichia coli isolates in Anhui province. Carbapenems could be the first choice for the treatment of infection caused by AmpC ?-lactamase producers.

Objective To explore risk factors for coronary artery aneurysms (CAA) in children with Kawasaki disease (KD),and reduce the rate of serious sequelae of cardiovascular diseases.Methods All the patients were diagnosed as KD in Children’s Hospital Affiliated to Medical College of Zhejiang University from January 2009 to January 2014.A total of 679 cases was included,and 42 with concurrent CAA,181 with concurrent coronary artery expansion (CAD),and 456 without coronary artery damage cases (non-coronary artery lesion,NCAL).Coronary artery damage was related to factors such as gender,age,fever,white blood cell count (WBC),hemoglobin (Hb),platelet count (PLT),erythrocyte sedimentation rate (ESR),C-reactive protein (CRP),alanine aminotransferase,serum potassium,plasma amino terminal brain natriuretic peptide precursor (NT-proBNP),and acrylic ball resistance.SPSS 18.0 software package was used for risk factor analysis.Qualitative data using chi-square test,was used to analyze the high risk factors of CAA group,and logistic multivariate regression analysis was also used.Results Chi-square test showed that male,with febrile days ＞ 14 d,NT-proBNP ≥ 1 000 ng/L,immunoglobulin resistance was more likely to have KD concurrent CAA (P ＜ 0.05).Multiple Logistic regression analysis showed that male [OR =4.092,95％ CI (1.514,11.060),P =0.004],febrile days ＞14 d [OR =12.436,95％ CI (4.599,33.631),P =0.000],NT-proBNP≥ 1 000 ng/L [OR =3.305,95％ CI (1.401,7.794),P =0.005],and immunoglobulin resistance [OR =3.842,95 ％ CI (1.562,9.453),P =0.000] were independent risk factors for KD concurrent CAA.Conclusions Male children,febrile days ＞ 14 d,NT-proBNP≥ 1 000 ng/L,and immunoglobulin resistance were independent risk factors of CAA.

To improve the students'! comprehensive quality and cultivate their innovative medical talents, we applied different forms of discussion teaching in different stages of the students. In the course of theory, the form of short discussion was adopted to improve the students'! interest and concentration. In preclinical practice stage, the method of problem centered discussion was used to consolidate the profes-sional knowledge of the students. Discussion in the clinical practice was based on the actual problem of diagnosis and treatment, in order to cultivate the students'!clinical thinking. Through the diversified practice of discussion teaching with positive teaching evaluation, students'!interest in oral and maxillofacial surgery and the quality of teaching were improved.

Objective To explore the acute toxic reactions of memantine in neonatal rats. Methods Based on Completely Lethal dose(LD_(100)) and median lethal dose (LD_(50))of memantine in SD neonatal rats acquired in a preliminary test of death dose, 60 neonatal rats were randomly divided into normal group which were given water injection intraperitoneally and 5 study groups which were given different doses of memantine intraperitoneally.LD_(50) was calculated with Bliss method and the toxic reactions of memantine were observed in all neonatal rats of 6 groups after administration of memantine. Results LD_(50) of memantine in SD neonatal rats was((74.386?2.811)) mg/kg with 95% confidence at the range of 59.334-93.257 mg/kg.Side effects occurred at 1-4 minutes after administration. Excitatory jitteriness,ataxia,decreased respiratory rate and passivity were usually observed in groups with a lower dosage (52.0 mg/kg,61.2 mg/kg,72.0 mg/kg);some of them also manifested side lying, cyanosis and respiratory failure.While neonatal rats with a higher dosage (85 mg/kg,100 mg/kg)mainly manifested visible symptoms of inhibition, respiratory failure,side (lying) and cyanosis.However,no jitteriness and ataxia were observed in them.The neonatal rats usually died around 1 hour after memantine administration;survival rats usually returned to normal 4-5 hours after administration.Conclusion There is a positive correlation between toxic reactions and the mortality with memantine dosage in neonatal rats.