Moyamoya disease is an uncommon chronic progressive occlusive cerebrovascular disease.It is mainly involved in the terminal internal carotid artery and the proximal portions of the anterior cerebral and middle cerebral arteries.Normally,there are differences between the conventional angiographic findings and the clinical manifestations and outcomes in moyamoya disease,while the perfusion imaging can provide important hemodynamic information.Currently,CT perfusion imaging and magnetic resonance perfusion imaging have been widely used in clinical practice.This article reviews the application of the two imaging techniques in moyamoya disease.

Objective:This study investigated the positive detection rate of cytoplasm thymidine kinase 1 (TK1) in lung cancer patients and the relationship of TK1 with clinicopathological features and prognosis. Methods:Sensitive chemiluminescence dot-blot assay was used to detect serum TK1 levels in 73 lung cancer patients and 56 normal control subjects. Results:The positive detection rate of TK1 was elevated in the lung cancer patients compared with the controls (P=0.006). The positive detection rate of TK1 was also correlated with distant metastases, but not with other factors, such as smoking, sex, lymph node metastasis, and pathology types. The 2 year survival of the patients with negative TK1 detection was significantly longer than that of the patients positively detected with the marker (P<0.001). Conclusion:Serum TK1, a new tumor marker, has potential applications in the diagnosis and prognosis of lung cancer.

Radiotherapy is one of the main means of clinical treatment for cancer, although the continuous updating and development of technology and equipment, adverse reactions and side effects can't be avoided yet.Improving the clinical efficacy of radiotherapy and reducing its adverse reactions are the currently facing subjects.A large number of clinical studies showed that using traditional Chinese medicines(TCM)in tumor radiotherapy had significant effects of improving and controlling the clinical symptoms, improving the life quality of cancer patients, improving the sensitization of radiotherapy, preventing the tumor metastasis and recurrence, as well as improving immune function and long-term survival, etc.Meanwhile, TCM also had significant roles in reducing the damage of radioactive inflammation, decreasing the bone marrow suppression to improve peripheral blood, and reducing the toxicity of digestive system, etc.The combination of radiotherapy and TCM provides a broader space for the comprehensive treatment of tumors.

Objective To discuss magnetic resonance imaging(MRI)scanning technology for ankle joint and its clinical value.Methods 42 cases of ankle joint trauma were examined with MRI using GPFLX coil.It was performed through sagittal T2WI,T2WI F/S,T1WI;coronal T2WI;axial T2WI.FOV was big enough for the whole trauma ankle joint.To make image clear,some soft materials should be placed between the trauma ankle joint and the GPFLX coil.The injured ankle joint was not allowed to move.The technology of No Phase Wrap is also necessary.Results The high quality rate was up to 90% for the ankle joint.Among 42 cases,6 had traumatic arthritis,14 had ankle joint contusion,9 had Achilles tendon rupture,6 had other structural injuries,4 had late degenerative change,and 3 had normal images.Conclusion MRI is a sensitive technique for injured ankle joint.It can accurately show the degree and location of ankle joint trauma.It has an important clinical significance in finding bone contusion and other structural injuries,and it is very helpful for treatment.

Objective To establish lung adenocarcinoma drug resistance cell strain induced by cis-diaminodichloroplatin and investigate the biologic characteristics and chromosome karyotype of cell strain.Methods Large dosage impact and step by step inducing method were used to establish A549 and SPC-A-1 drug resistance cells: A549/CDDP and SPC-A-1/CDDP.MTT was used to detect the cell drug resistance index. Bioluminescence was used to detect the energy metabolism.Cell cycle was analysed by flow cytometer.The differential staining technique and SKY were used to analyze the chromatosome of A549/CDDP and SPC-A-1/CDDP.Results The drug resistance index of A549/CDDP and SPC-A-1/CDDP were 14.0 and 12.0. The content of ATP,ADP and AMP decreased significantly.The cell cycle of A549/CDDP and SPC-A-1/CDDP were of no notable changes.The results of the differential staining technique and SKY showed that the chromatosome of A549/CDDP and SPC-A-1/CDDP had several derivative chromosomes.Both cells had the same derivative chromosome: der(21)t(21;22).Conclusion CDDP can induce lung adenocarcinoma cell strain drug resistance.The derivative chromosome,including der(21)t(21;22) may have relationship with the drug resistance of cell strains.

Objective To analyze the unbalanced state of hereditary material of squamous carcinoma, adenocarcinoma and other lung cancers. Methods Fifty-five patients suffered from lung cancer were included in this study, including 24 of squamous carcinoma, 13 of adenocarcinoma, 5 of adenosquanous carcinoma, 8 of bronchioalveolar carcinoma, 4 of small cell lung cancer and 1 of atypical carcinoma. The technology of comparative genomic hybridization (CGH) was used. The normal DNA was obtained from 20 healthy male volunteers. Results The common extension region of squamous carcinoma was 2q, 5p, 11q and 22q, and the common deletion region was 1p, 4q, 5q, 6q, 8p, 9p, 10q, 11p, 13q, 18q and 21q. The common extension region of adenocarcinoma was 5p, 8q and 11q, and the common deletion region was 10p and 19. Conclusion The hereditary material of squamous carcinoma, adenocarcinoma and other lung cancers was unbalanced. The extension and deletion of chromatosome were the base of the occurrence of different lung cancer.

Objective:To study the relationship between Fc? receptor ⅢB(Fc? RⅢB) genotype and severe chronic periodontitis or generalized aggressive periodontitis.Methods:DNA from 35 subjects with severe chronic periodontitis,25 with generalized aggressive periodontitis and 95 of periodontal healthy controls were sampled by buccal swab and extracted using Chlex-100 method.Polymerase chain reaction with sequence specific primers (PCR-SSP)was carried out to determine the Fc?RIIIB genotype.The data were analyzed with ? 2 test. Results: NA1/NA2 heterozygote was the predominant genotype in the 3 groups.No statistically differences of Fc?RIIIB genotype distribution were found between any two of the stuied groups.The gene frequencies of NA1 and NA2 were 0.6 and 0.4 respectively.Conclusion:No direct relationship exists between Fc?RⅢB genotype and the occurrence of severe chronic periodontitis and generalized aggressive periodontitis according to the present study.

OBJECTIVETo provide quantitative foundation for the diagnosis of atlanto-axial rotatory subluxation by analyzing the various imaging features of normal atlanto-axial joints in neutral position and rotary functional position on the MSCT images.

METHODSForty-one normal volunteers were examined by CT on the atlanto-axial joint in neutral position and rotary functional position. By the observation and measurement of atlanto-dental interval (ADI), lateral atlanta-dental space (LADS), VBLADS and rotating angle of atlas on dentate (RAAD), the imaging manifestations and anatomical characteristics were analyzed and compared. In order to compare VBLADS and RAAD and make a correlation analysis between different age groups, 51 normal volunteers were divided into two groups: age younger than 45 years old group and age older than or equal to 45 years old group.

RESULTSThe dens in neutral position deviated in an angle range of (3.22±0.89)°. The articular facets of lateral atlantoaxial joint in rotary functional position had rotatory displacement and the range of the relative rotation angle was (33.85± 2.79)°. Through the correlation analysis of matching data, it could be concluded that there was no correlation between atlantoaxial relative rotation angle and VBLADS within a certain range. There were statistically differences of atlantoaxial relative rotation angle in rotary functional position between two groups.

CONCLUSIONMSCT imaging in rotary functional position can clearly show the anatomical structure and rotation function of a normal atlanto-axial joint, so as to provide a theoretical basis for the diagnosis of atlanto axial rotatory subluxation.

Adult ; Aged ; Atlanto-Axial Joint ; diagnostic imaging ; Female ; Humans ; Male ; Middle Aged ; Multidetector Computed Tomography ; methods ; Rotation

Ablation Techniques ; adverse effects ; Animals ; Bone and Bones ; pathology ; Disease Models, Animal ; Extremities ; Swine

Objective To investigate the effects of human cytomegalovirus ( HCMV) persistent in-fection on the central nervous system of BALB/c mice.Methods Thirty specific-pathogen-free mice of 6-8 weeks old were randomly divided into three groups including HCMV infected group , inactivated HCMV group and human embryo fibroblast ( HF) control group .Each mouse in the three groups was intraperitoneally inoc-ulated with 1.8 ×107 PFU of HCMV, 1.8 ×107 PFU inactivated HCMV and 1 ×105 HF cells, respectively. All mice were housed in microisolator cages for three months and their behavior and body weight were ob -served.Then three tests including autonomic activities test , Morris Water Maze and step-down passive avoid-ance task were performed on all mice to evaluate the changes of their behavior .Cerebral cortex tissues were collected from all mice to detect HCMV and to conduct polymerase chain reaction (PCR) analysis.Brain tis-sues were stained by HE method to evaluate the pathological damages .Transmission electron microscope was used to observe the ultrastructure of neuron cells and the existence of virus particles .Results (1) The body weight of mice showed no significant differences among the three groups ( P>0 .05 ) .( 2 ) The frequency of autonomic activities were decreases in HCMV infected group in comparison with other two groups , but there was no significant differences among the three groups (P>0.05).(3)The place navigation test demonstra-ted that the escape latency of mice from HCMV infected group as well as HF group showed significant differ -ence after training for different periods of time (P<0.05).The escape latency of mice with HCMV infection was much longer than that of other two control groups (P<0.05), but the differences between two control groups were not significant (P>0.05).Compared with the mice in two control groups , the mice in HCMV infected group showed a lower frequency of crossing the quadrant where the platform had been located on pre -vious trials in the probe trial test (P<0.05).Moreover, the time of first crossings was also longer than that of mice from two control groups (P<0.05).(4)In the learning phase the mice from HCMV infected group showed a high frequency of mistakes in comparison with that from two control groups in step -down passive avoidance task (P<0.05).After 24 hours, the frequency of mistakes was decreased in each group , and the differences were significant (P<0.05).The latency was very shorter in mice from HCMV infected group than that observed in two control groups (P<0.05).(5)The HCMV infection was identified in six mice from HCMV infected group .And the positive HCMV UL83 gene could be only detected in HCMV infected group as indicated by PCR analysis .(6)The pathological changes including cell swelling , loosen cytoplasm, decreased cell layers and vacuolization were observed in the cerebral cortex and hippocampus of mice from HCMV infected group .HCMV-specific intracytoplasmic inclusion bodies and herpes virus like particles were detected by using transmission electron microscope .No obvious abnormalities were observed in two control groups.Conclusion HCMV persistent infection could damage the central nervous system of mice .The au-tonomic activities of mice with HCMV persistent infection were not affected , but the learning and memory ca-pability of them were damaged at some extent .