The concentrations of lactoferrin (LF) in colostrum during the first 8 days and mature milk during the first 4 months of postpartum were determined by electroimmunodiffusion. Meanwile a sensitive enzyme linked imm-unosorbent assay for measurements of LF in neonatal faeces were established. The results showed that human milk, especially colostrum, was rich in LF. The colostrum contained 6.88 ? 3.10mg/ml (M?SD) aad mature milk 3.18 ? 0.37mg/ml LF. LF in human milk was the highest during the first 3 days of lactation with a decline in concentration as lactation proceeds although a wide variation in concentration was noted among different individuals.LF in neonatal faeces showed significant difference in different feeding manners. LF level in the breast-fed infants was 19 times higher than that in the bottle-fed infants. The LF percentage detected in the former was 100% and the latter only 60%. The dynamic variations of LF in faeces of breast-fed infants during the first day of life were found to be relative to LF level in mother'milk.These data demonstrated the influence of high LF level in mother'milk on the concentrations of LF in breast-fed infants' intestine.Furthermore a few amounts of LF was found in meconium and its percentage detected was 35%. This suggested that the production of endogenous LF began from foetus period, but was limited during the first day of life. LF ingested by the breast-fed infants were not completely destroyed and thus may supplement the insufficiency of the endogenous LF in the newborn infants' intestinal tract.

OBJECTIVE:To develop a RAM(Restricted-access media)-HPLC for the determination of plasma concentration of carbamazepine,and to compare the determination results with those determined by FPIA method. METHODS: The plasma samples were centrifugalized,then the venous plasma samples were determined by FPIA and RAM-HPLC,respectively,while the fingertip plasma sample was determined by RAM-HPLC alone. The chromatographic column was Hisep shielded hydrophobic phase analytical column,and the mobile phase consisted of ammonium acetate buffer-acetonitrile (88∶12) at a flow rate of 1.5 mL?min-1. The detective wavelength was set at 280 nm;the sample size was 20 ?L and the column temperature was kept at room temperature. RESULTS: A good correlation was noted between the carbamazepine concentration in venous plasma sample determined by FPIA and carbamazepine concentrations in either venous or fingertip plasma determined by RAM-HPLC,with the correlation coefficients being 0.989 and 0.995,respectively,but the differences were significant(P0.05). CONCLUSION: Both RAM-HPLC and FPIA are applicable for the determination of carbamazepine concentration,but FPIA is more suitable for routine monitoring while RAM-HPLC is more suitable for the related research and monitoring of specific cases. Both methods shows good reproducibility and in which the samples don't have to be pretreated.

AIM: To explore the changes of L-selectin expression on peripheral blood polymorphonuclear leukocytes (PMNs) and their significance in rats with acute lung injury (ALI). METHODS: ALI model in rat was established by intravonous injection of E. coli endotoxin (ET). The expression of L-selectin on peripheral blood PMNs was measured by immunofluorescence and flow cytometry.RESULTS: The contribution of L-selectin fluorescence signal was on the surface of PMNs membrane. The expression of L-selectin on poeripheral blood PMN was significantly lower at 5 min after injection of ET and the lowest during 15 min to 30 min, then gradually increased, but the expression of L-selectin on PMN was lower at 60 min after injection of ET than that of control animal.CONCLUSION: In physiological state, L-selectin were expressed on the surface of PMN membrane. The protein expression of L-selectin on PMNs reduced rapidly after injection of ET and the lowest at 15 min, then gradually increased. L-selectin may play a role in the development of ALI.

AIM: To investigate the adhesion between lymphocytes and extracellular matrix (ECM) in delayed-type hypersensitivity (DTH) in mice as well as the regulatory effect of several cytokines on it. METHODS: DTH was induced by the application of picryl chloride (PCl) to mice that had been sensitized twice by PCl, followed by isolating the spleen lymphocytes at different times. The adhesion of spleen lymphocytes to fibronectin or typeⅠcollagen were examined. In some cases, spleen lymphocytes obtained at 18 h were incubated with various concentrations of IL-2, IFN-?, and TNF-? for 4 h at 37℃ before the adhesion assay. T cells purified from the spleen lymphocytes were also used for the assay in the presence of TNF-?. RESULTS: The adhesion of spleen lymphocytes to ECM began to increase at 6 h after PCl challenge, reached a peak at 18 h, then decreased and returned to normal level at 36 h. IL-2 significantly increased the adhesion of lymphocytes to ECM at a dose of 1?10 5 U/L and TNF-? enhanced the adhesion in a concentration-dependent manner. The combined use of TNF-? and IFN-? showed a significantly enhanced adhesion. TNF-? was more effective to accelerate the adhesion of spleen T Cells than that of total spleen lymphocytes. CONCLUSION: In the process of DTH, the adhesion of lymphocytes to ECM was regulated by several cytokines and associated with the development of inflammation.

Artificial ureter has been studied for nearly 60 years, however it is still in experimental stage. So far there are no commercially available products for clinical use except autologous intestines loop even though many progresses have been made. Each approach for making artificial ureter has its own inherent advantages, disadvantages and limitations. But some approaches have shown great potential to be used as ureteral substitutes. Preventing the conduit from salt depositing and ensuring a watertight anastomoses with native tissues are the key factors in designing and manufacturing the prosthetic ureter. Along with scientific and technical improvement, artificial ureter will be successfully used in clinical therapeuticsin in the very near future.

Combined with the routine work of preventing and treating diseases, the construction of a clean and honest administration in stomatological hospital would be beneficial to raise the administrative staff's awareness of the constructive characteristics of a clean and honest administration in their work belief and methods to enhance the work initiative and effectiveness.

Through the questionnaire survey of community residents,the paper studies the factors influencing users' acquisition of health information from libraries in medical universities and colleges and provides reference and suggestions for socialized services of these libraries.As indicated by the results,the influencing factors include the distance,motivation,environment,limitation and time.On this basis,it proposes solutions,including the improvement of the service mechanism,the utilization of advantages of medical library resources and the establishment of the service system for community residents centering on medical libraries.

Objectives To grasp the situation and fluctuation tendency of Keshan disease in Qujing City,and to scientifically evaluate the control effect of Keshan disease.To provide scientific basis for elimination of Keshan disease in the 12th Five-Year Plan.Methods Cardiomyopathy cases of the past three years were searched,screening suspected cases of Keshan disease.And according to the National Surveillance Program of Keshan Disease (Trial),In Qujing City,2 Keshan disease counties (Huize,Qilin) were selected to carried out focused monitoring,1-2 endemic townships with more patients were selected based on total numbers of cases in each county,1 village with more patients was selected in each township as a monitoring site,the residents of the monitoring sites were examined clinically and electrocardiographic (ECG) tracings recorded and suspicious patients were taken chest radiograph and color Doppler ultrasound echocardiography.Diagnosis was based on the Diagnostic Standard for Keshan Disease.Results Totally 300 cases of cardiomyopathy were found,10 cases were suspected Keshan disease,0 cases of Keshan disease was diagnosed in 2009-2012.In the three villages of the two counties monitored,3 cases of potential Keshan disease were detected of the 1 505 villagers,the detection rate was 0.20％;62 abnormal ECG were found,the abnormal rate was 4.12％.Chest X-ray examination was done in 3 people,cardiothoracic ratio of 0.50 or less were 2 people,and 0.51-0.55 was 1 person.Conclusion The prevalence of Keshan disease in Qujing City is low,but the pathogenesis of Keshan disease remains,therefore,monitoring and intervention of children Keshan disease should be strengthened.

Objective:To compare the feasibility,complications and advantages of the nose-intestine tube and the jejunostic tube in early enteral nutrition after double incisions for the therapy of esophageal cancer.Methods: 52 postoprative patients were divided into three groups.The nose-intestine tube group(A group,n =16) and the jejunostic tube group(B group,n = 21) received the enteral nutrition(Nutrison Fibre) and the conrtrol group(C group,n = 15) received intravenous isotonic glucose solution and oral liquid diet after the bowel movement recovery.The blood glucose,the function of liver and kidney,electrolytes and nutritional status were observed.The recovery of bowel movement and other digestive symptoms such as abdominal pain,distention,diarrhea,nausea and vomiting were observed during the period of study.Some complications such as acute intestinal obstruction,pnumonia and rhinolaryngitis were observed during the period of study.Results: The time for placing the jejunostic tube was faster than that for placing the nose-intestine tube(P