Objective To establish a rapid determination method for the exchangeable and reducible manganese.Methods In NH4Ac solution,manganese was determined by flame atomic absorption spectrophotometry.Results The linear ranges was 0.40-2.00 ?g/ml for exchangeable manganese and 100-5.00 ?g/ml for reducible manganese respectively.The detection limits were 0.012 ?g/ml for exchangeable manganese and 0.0078 ?g/ml for reducible manganese respectively.The RSDs were lower than 1.2%.The recovery rates were 100.2% for exchangeable manganese and 98.8% for reducible manganese respectively.The correlative coefficients were 0.9992 for exchangeable manganese and 0.9995 for reducible manganese respectively.Conclusion This method is rapid,simple,sensitive and accurate.

0.05). The total effective rate of TCM syndrome effect in the treatment group was 91.4% and that was 65.7% in the control group, with a significant difference (P0.05), whilst in the control group, 34% of cases had nausea and poor appetite.Conclusion Nourishing the liver and kidney is an effective therapy for children with ADHD. It can significantly improve the symptoms and related indices.

Objective To evaluate the scales of psychological distress and pain in patients with advanced cancer and the impact of pain on the anxiety and depression.Methods Seventy-four patients with advanced cancer were observed.The hospital anxiety and depression(HAD)scale and the brief pain inventory(BPI)were completed either by patients or under the assistance of the investigator.Results HAD-A(anxiety)and HAD-D(depression)scores were(7.68±4.09)and(7.19±3.38),respectively.Significant correlations were found between pain severity and HAD-A or HAD-D(P＜0.001)by univariate correlation analysis.Furthermore,significant associations were shown between HAD-A and six"pain interference in the patients'functional ability"items(P＜0.001),and between HAD-D and pain interference to"mood"or"relations with other people"(P＜0.01).Conclusions Quantitative analysis of psychological distress have shown that patients with advanced cancer are doubtful cases for both anxiety and depression and need psychological management.Anxiety and depression is not only associated with pain intensity directly,but also with pain interference in the patients'functional ability indirectly.

OBJECTIVE:To compare the effects of omeprazole and esomeprazole,two kinds of proton pump inhibitors,on clopidogrel platelet inhibition following cardiac stents implantation.METHODS:Totally 180 patients with coronary artery disease underwent percutaneous coronary intervention (PCI) at the Zhujiang Hospital of Southern Medical University from June 2008 to May 2009 were selected,including 83 males and 97 females.All patients were randomly divided into 3 groups,omeprazole + clopidogrel + aspirin group (OCA group,receiving omerprazole 20 mg/d),esomeprazole+clopidogrel+aspirin group (ECA group,receiving esomeprazole 10 mg/d),and control group (No proton pump inhibitor),with 60 patients in each group.In addition,all patients received a 300 mg clopidogrel and 0.1 g aspirin prior to PCI,and received 75 mg/d clopidogrel and 100 mg/d aspirin treatment for 1 week after PCI.Blood samples from patients were obtained from cubital vein before and at 1 week after adminstration,respectively.The vasodilator stimulated phosphoprotein phosphorylation state and platelet reactivity index (PRI) were calculated by flow cytometric assay.RESULTS:The PRI had no significant difference before administration (P>0.05),which was obviously decreased at 1 week after administration (P<0.05),especially lowest in the control and ECA groups (P<0.05).However,the PRI differences between the control and the ECA group had no significant (P>0.05).CONCLUSION:The administration of omeprazole rather than esomeprazole is associated with impaired clopidogrel platelet inhibition.Esomeprazole can be used as one of the preferred proton pump inhibitor in curing gastrointestinal bleeding caused by anti-platelet therapy following cardiac stents implantation.

With the improvement of living standard, the requirement of artificial joint material is ever increasing. The development of meted artificial joint, high polymer artificial joint and ceramic-made artificial joint, which are widely used in joint replacement, are introduced. Advantages and disadvantages of these materials are discussed, as well as the required improvement to these three materials. Development on metal-to-metal hip resurfacing arthroplasty and its clinical application is introduced. Now that no material with good biocompatibility, good biomechanical compatibility, good biological aasociativity and satisfied life-span has yet been found, compound material artificial joint may be the focus of the research in future.

The phospholipid content in BALL markedly increased (P0.01) in septic rats induced by cecal ligation plus punctures as compared with non-septic control. Meanwhile, the lamellar inclusion bodies in type Ⅱ alveolar epithelial cells were empty observed in electro-microscopic examination. Authors suggest that the changes mentioned above is a compensatory response related to stress in early stage of sepsis.

Objective By analyzing the dgug-resistance of Acinetobacter baumannii got from clinic in recent 3 years to study the therapy method of pan resistant bacteria.Method Bacteria was got from sputum or trachea pipe of patients normally,identified with ATB and test 14 common antibiotic drugs by K-B method.Result All the drug-sensitive test results of 248 bacteria in 3 years show that drug-resistance rate is high(40.3%~89.5%)except for IPM,IMI and sulbactam(11.7%~15.3%),and pan resistant bacteria(resist 4 or more kinds of antibiotic drugs)is 37.1%.Conclusion Acinetobacter baumannii is the main pathogen of lower respiratory tract infection.The high picks out rate and high drug resistance get great trouble to therapy.We need to make a strict standards of asepsis operation,strengthen monitoring the drug resistance and advocate to use antibiotic drugs reasonably,so that we can prevent and control the prevalence of Acinetobacter baumannii.

OBJECTIVE To study the antimicrobial agents resistance of Staphylococcus aureus isolated from clinic.METHODS To test the sensitivity of 20 common antimicrobial agents by K-B.MRSA was detected by cefoxitin disk;and the inducible resistance of clindamycin was detected by D-test.RESULTS The drug resistance rate of this term of S.aureus was high.Except vancomycin,minocycline,teicoplanin,nitrofurantoin and rifampin,the drug resistance rate was higher than 42.6%.From 312 strains of S.aureus 209 strains of MRSA were detected,the rate was 67.0%.The structural antimicrobial resistance rate of clindamycin was 29.5%,the detection rate of inducible resistance was 51.4%.CONCLUSIONS The antimicrobial resistance of S.aureus is increasingly severe.The high positive results of MRSA and D-test make great trouble to therapy,and should have taken more attention of clinic.

OBJECTIVE To analyze the etiological characteristics and risk factors of burn wound infection to provide scientific basis for preventive measures. METHODS The pathogens and risk factors of wound infection of burn patients were investigated. RESULTS Among 341 burn patients form Jan 2005 to Dec 2007, 257 patients were cultured positively in their wound surface with the total infection rate 86.0%. In the early period of infection, Gram-positive cocci were the main pathogens in preinfection, then Gram-negtive bacilli and fungal infection increased. Most of pathogens were opportunistic. From d4 to d10 after burn was the dangerous period of infection. Infection rate was related to the sizes of burn area. CONCLUSIONS Visiting hospital, debridement and takeing specimens to culture in time, correct antibiotics treatment, sterile environment and aseptic operation are the effective measures to reduce the wound infection.

OBJECTIVE To study the clinical characteristics and current status of the drug resistance of Burkholderia cepacia(BCE).METHODS Drug sensitivity test was performed on BCE with K-B method isolated from clinic specimen from 2006 to 2007 and the patients′ data were analyzed.RESULTS The pathogens were mainly isolated from sputum(18 strains,39.1%) and blood(9 strains,19.6%).ICU was the common department(28 strains,60.9%);all strains of BCE were resistant to ampicillin/sulbactam.The resistance rate to 3 kinds of aminoglycosides(amikacin etc),ciprofloxacin,piperacillin and imipenem were 63.0-84.8%.The resistance to sulfamethoxazole compound,ceftazidime,cefepime piperacillin/tazobactam,cefoperazone/sulbactam and meropenem were less than 40.0%.CONCLUSIONS Serious underlying diseases,frequent invasive operation and drug abusement were the main causes of BCE infection.Meropenem,sulfamethoxazole compound,ceftazidime,piperacillin/tazobactam and cefoperazone/sulbactam could be selected to treat BCE infection.