BACKGROUND:The colagen and chitosan composite membrane made at a certain percentage can reduce the positive charge of chitosan, improve the adsorption force of chitosan, promote the cel adhesion and growth, migration and proliferation, and enhance the biological properties of chitosan, which has become a very excelent biological material. OBJECTIVE:To prepare chitosan-colagen composite membrane and observe its histocompatibility with corneal stroma layer. METHODS:Chitosan-colagen composite membrane was prepared. Totaly 16 New Zealand rabbits were randomly divided into experimental and control groups. The chitosan-colagen composite membrane was implanted into right eye cornea stroma bag in the experimental group, and the chitosan membrane was implanted into right eye cornea stroma bag in the control group. The slit lamp microscope, anterior segment optical coherence tomography and histological observation were conducted after transplantation. RESULTS AND CONCLUSION: Slit lamp microscope observation results showed that after 8 weeks of transplantation, degradation infiltration was visible in the center of the diaphragm, crease buckling was not obvious in the experimental group; the diaphragm was infiltrated completely and crease buckling was notable in the control group. The anterior segment optical coherence tomography results showed that at the 6thweek after transplantation, the composite membrane boundary was fuzzy, its density was very close to the normal corneal tissue, and the corneal shape recovered to normal in the experimental group. The histological findings showed that after 8 weeks of transplantation, in the experimental group, the membrane surface was degraded a little; biodegradable materials were fused with the corneal stroma; there were a smal amount of inflammatory cels infiltrated on the corneal stroma around the iris. In the control group, the membrane degradation was greater than that of the experimental group; degradable substances intertwined and fused with corneal stroma; there were more inflammatory cels infiltrated on the corneal stroma around the iris. These results demonstrate that chitosan-colagen composite membrane has degradability and good histocompatibility.

Objective To study the influence of different dosages of atorvastatin in acute coronary syndrome female patients on vascular endothelial growth factor(VEGF) and brain natriuretic peptide(BNP).Methods 60 female cases of ACS patients were divided into A and B group.30 patients in the A group were given atorvastatin 20mg per night,while the other 30 patients in the B group were taken 40mg per night.The patients were followed four weeks by evaluating serum VEGF and BNP concentration.Results After treatment for four weeks,the patients in the two groups showed significant reduction in VEGF and BNP,however,the therapeutic group showed significant difference between the two groups.Additionally,the B group further showed significant reduction in VEGF and BNP.Conclusion Using high-dose atorvastatin lowers VEGF and BNP significantly,which could reduce female cardiovascμlar events.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Sciatica ; therapy

Through the study and analysis of doctor-patient relationship in the morden medical conditions in China,in this article,the author,combing with her medical praltice for many years,points out some principal problems existing in the relationship,and also puts forward some feasible countermeasures which will be helpful to set up harmonious doctor-patient relationship and ensure the durable and normal development of Chinese medical and healthy cause.

BACKGROUND:Effects of pedicle screw placement on fracture reduction or stability of mechanics are influenced by various factors. Pedicle screw fixation failure is mainly due to fracture, loosening and fatigue. The main influential factors for biomechanical stability of pedicle screw are length and diameter. The research on the depth is less. OBJECTIVE:To analyze the relationship between pedicle screw placement depth of vertebral body and the fixed stability based on the biomechanics. METHODS: A model of single vertebral compression fractures was made in 15 pigs aged 5 months on L2 vertebral specimen. According to the length of anteroposterior diameter, vertebral pedicle screws were inserted in different depths (80%, 90% and 100% of anteroposterior diameter). After fixation, specimens were loaded 10 000 times at the frequency of 0.5 Hz (340±125) N on a WDT-10KN type universal material testing machine. Ranges of motion at anteflexion, backward extension, left bending and right bending and the maximum axial pulout force were measured in each group, and the difference of intergroup data was compared. RESULTS AND CONCLUSION:(1) Ranges of motion at anteflexion, backward extension, left bending and right bending in each group were significantly smaler in the 100% and 90% groups than in the 80% group (P < 0.05), and above parameters were smaler in the 100% group than in the 90% group (P< 0.05). (2) After fatigue test, the maximum axial pulout force was significantly larger in the 100% and 90% groups than in the 80% group (P< 0.05), and above data were significantly larger in the 100% group than in the 90% group (P < 0.05). (3) Results indicate that the depth into the vertebral body was significantly associated with its fixed stability. The deeper the depth into the vertebral body, the stronger the vertebral stability was: 100% group > 90% group > 80% group.

normal morphology + cervicitis).

Objective To remove formaldehyde of the low concentration in the indoor air and purify the indoor air. Methods The concentration of formaldehyde was determined by MBTH spectrophotometry and the removal efficiency of low concentration formaldehyde in the indoor air by using sodium sulfite, sodium bisulfite, ammonium sulfate, ammonium chloride, ammonium molybdate and potassium permanganate was tested. Results As the concentration of formaldehyde was at 1 mg/L, 10 mg/L and 100 mg/L respectively, the removal rate of formaldehyde of sodium sulfite, sodium bisulfite and potassium permanganate was 15.9%, 74.7% and 93.5% respectively. On the acidity condition or alkalescence, potassium permanganate was also effective in removing of the different concentration formaldehyde was 23.8%, 74.7% and 93.5%. Ammonium molybdate and potassium permanganate could remove the formaldehyde by 25.9% and 35.7% when the concentration of formaldehyde was at 10 mg/L and 100 mg/L. Ammonium sulfate or ammonium chloride could not effectively remove the low concentration formaldehyde and the removal rate was under 7.0%. Conclusion On the acidity condition or alkalescence, potassium permanganate is effective in removing of the low concentration formaldehyde in the indoor air.

Objective To study the blood pressure and cardiovascular conditions in kidney transplant recipients in order to prevent the cardiovascular complications in such patients. Methods The blood pressure and ECG were monitored day and night by noninvasive means for 86 patients after transplantation.The results were analysed and compared with the pretransplantation studies. Results Hypertension occurred in 55 out of 86 patients.There was a significant difference of average blood pressure and the occurrence of abnormal ST segment or T wave between day and night whereas no significant change was observed in blood CsA concentration,blood creatinine,serum cholesterol,blood sugar,triglyceride and K+,N+,Cl-,TCO 2,Ca 2+ ,etc. Conclusions Cardiovascular complications in kidney transplant recipients is related to the high blood pressure during night,the normal day-night blood pressure rhythm being violated.It is not related to the immunosuppresives used.

Objective To study whether omeprazole induces gastric epithelial cells apoptosis and analyse the possible mechanism. Methods The cell cycle and apoptosis ratio of SGC 7901 cell line were determined by flow cytometry in the presence of omeprazole, meanwhile, TUNEL positive cells from gastric mucosa were counted after incubated with omeprazole for 72 hours. Expression of p53、 GADD 45 、 p15,16,18,19 and bcl 2 were stained by immunohistochemical assay and ATPase activity was tested. Results significant alteration with decreasing percentage of G 1 phase and increasing percentage of G 2 phase of SGC 7901 cell line could be detected after incubating 24 hours with omeprazole at double clinical doses . An apoptosis peak appeared in flow cytometry after incubating 72 hours with omeprazole. However, no genetic products such as p53, GADD 45 , p15,16,18,19 and bcl 2 were seen. Concentrations of ATPase were markedly increased from 0.326 0.387 before incubated with omeprazole to 0.054 0.103 after incubated with omeprazole. TUNEL positive reaction cell counts in fresh mucosa was also increased after incubated with omeprazole for 72 hours( P

0 05). It is suggested that: ①When foot brace fixed the femur at inward rotation of 0?, 15?and then 15? after moving body position, and ROI was near the neck, BMDs of the neck and the trochanter were not affected in either of the three positions, with femoral neck with highest BMD; ②When BMD value of the femoral Ward′s triangle is taken as the index, it is better to take the mean of 3～5 scannings at one position.