1.A randomized trial of cilostazol versus ticlopidine for anti-platelet therapy after coronary artery stenting for prevention of restenosis
Bin HONG ; Juying QIAN ; Bing FAN
Chinese Journal of Interventional Cardiology 1993;0(03):-
0.05).Conclusion Aspirin plus cilostazol showed comparable antithrombotic effect as aspirin plus ticlopidine after elective coronary stenting and may serve as an alternative treatment for patients intolerable to ticlopidine.
2.Impact of lesion length on restenosis after intracoronary stenting
Bin HONG ; Juying QIAN ; Bing FAN
Chinese Journal of Interventional Cardiology 1993;0(03):-
20 mm has negative correlation with restenosis.
3.Effect of MNNG on Ornithine Decarboxylase Activity in Cells from Adult Schistosoma japonicum
Hong FAN ; Min HUANG ; Qian WANG ; Manjun LI
Chinese Journal of Parasitology and Parasitic Diseases 1987;0(01):-
Objective To observe the change of ornithine decarboxylase(ODC) activity in cells from adult Schistosoma japonicum after the cells were treated with N\|Methyl\|N\|Nitro\|N\|Nitrosoguanidine (MNNG). Methods The cells were treated with MNNG at a concentration of 3 ?g/ml for 48 hours after the cells being incubated for one week. The cells were then cultured with RPMI\|1640 containing 10% calf serum. ODC activity was detected with spectrophotography. Results ODC activity rose significantly in two to three weeks after the cells were treated with MNNG. Conclusion There was ODC activity in cells from adult S.japonicum and MNNG has an effect to reinforce ODC activity in the cells.
4.Corneal histopathological changes and interleukin-6 level in aqueous humor of a rabbit model of penetrating corneal trauma combined with seawater immersion*
Qinhua FAN ; Qian CHEN ; Jin HONG ; Zhiqiang ZHANG ; Yanqiu YANG
Chinese Journal of Tissue Engineering Research 2011;15(50):9381-9384
BACKGROUND: Following trauma caused by seawater, cells often exhibit special pathological changes because of the special physico-chemical properties of seawater.OBJECTIVE: To observe corneal histopathological changes and interleukin-6 level in aqueous humor of a rabbit model of penetrating corneal trauma combined with seawater immersion.METHODS: The rabbit eye models of penetrating corneal trauma caused by firecrackers were established in 16 adult healthy grey rabbits. A 3-mm whole-layer incision was made in the cornea. The right eyes served as experimental sides and the left eyes served as controls. Seawater was injected into the aqueous humor of the right eyes via the corneal incision. The eye surface was flushed with seawater for 30 minutes. Physiological saline was used for the left eyes. RESULTS AND CONCLUSION: Optical microscopy results showed that at 1, 2, 3 days after model establishment, corneal cells on the experimental side exhibited severe necrosis and abscission, obvious swelling of substantia propria layer complicated by cellular infiltration. At 1 and 2 days after model establishment, the pathological changes on the control side were the same as the experimental side, but they were mild, but at 3 and 5 days, they were obviously alleviated. At 1, 2, 3 days after model establishment, interleukin-6 level in aqueous humor was significantly higher on the experimental side than on the control side (P < 0.05). These findings suggest that the degree of injury on the experimental side was more severe than that on the control side, indicating that seawater may be an important causative factor of corneal injury.
5.Research advances of miR-126 and ophthalmic diseases
Ning-Ning, GAO ; Fan-Qian, SONG ; Hong-Yan, GE
International Eye Science 2017;17(6):1066-1068
Up to now, a variety of microRNAs have been found in a number of studies, that specifically expressed in retinal neuroepithelial, lens, cornea and retinal pigment epithelium, in which miR-126 plays a certain role in the proliferation of tumor cells, the development of thymus lymphocytes and cardiovascular diseases.Some researches show that miR-126 has certain correlations with the formation of corneal neovascularization, the development of diabetic retinopathy, and the immune system related eye disease.In this paper, the current miR-126 in the role of eye disease mechanism and research progress were reviewed.
6.Effect of uric-acid-lowering therapy on progression of chronic kidney disease: A meta-analysis.
Ye-Fang, ZHANG ; Fan, HE ; Hong-Hui, DING ; Wei, DAI ; Qian, ZHANG ; Hong, LUAN ; Yong-Man, LV ; Hong-Bing, ZENG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2014;34(4):476-81
The efficacy and safety of uric-acid-lowering therapy (UALT) on slowing the progression of chronic kidney disease (CKD) accompanied by hyperuricemia were assessed. We searched Cochrane Library, PubMed, EMbase, CNKI, Wanfang and Vip databases up to November 15, 2012 for randomized controlled trials (RCTs) which compared the effect of UALT to control therapy in hyperuricemic patients secondary to CKD, and then performed quality evaluation and meta-analysis on the included studies. Seven RCTs involving 451 cases were included. UALT delayed the increase of serum creatinine (MD=-62.55 μmol/L, 95% CI: -98.10 to -26.99) and blood urea nitrogen (MD= -6.15 mmol/L, 95% CI: -8.17 to -4.13) as well as the decrease of glomerular filtration rate [MD=5.65 mL/(min·1.73 m2), 95% CI: 1.88 to 9.41], decreased systolic blood pressure (SBP) (MD= -6.08 mmHg, 95% CI: -11.67 to -0.49), and reduced the risk of the renal disease progression (RR=0.30, 95% CI: 0.19 to 0.46). However, there was no statistically significant difference in 24-h urinary protein quantity and diastolic blood pressure (P>0.05). We identified that UALT could delay the progression of CKD with secondary hyperuricemia. And this also indirectly proved that hyperuricemia was a risk factor for the CKD progression.
7.Analysis of cage migration after transforaminal lumbar interbody fusion
Fengdong ZHAO ; Wei YANG ; Junhui LIU ; Jian WANG ; Haixiao CHEN ; Zhenghua HONG ; Yu QIAN ; Dengwei HE ; Shunwu FAN
Chinese Journal of Orthopaedics 2012;32(10):922-927
Objective To investigate characteristics of cage migration after transforaminal lumbar interbody fusion (TLIF) and related risk factors.Methods A retrospective study was conducted to review cage migration in 512 patients who had undergone TLIF procedure from January 2010 to June 2011 in 5 spinal research centers.There were 255 males and 257 females,aged from 37 to 77 years (average,54.7 years).All patients were followed up at 3,6,12 months after operation.The clinical outcomes were evaluated using the visual analogue scores (VAS) and Oswestry disability index (ODI).X-rays and 3D CT scans were used to analyze the incidence and related risks factors of cage migration in these patients.Results Cage migration was found in 6 of 512 patients,the total incidence was 1.17%.Significant difference was found between each center.Cages with different shapes had different incidence.The analysis showed that the incidence of migration of rectangular-shaped cage (3.11%,5/161) was significantly higher than that of kidney-shaped cage (0.28%,1/351).The cage in double-segment TLIF (5.75%,5/87) was easier to migrate than that in monosegment TLIF (0.24%,1/425); furthermore,linear type endplate(3.50%,5/143) was remarkably easier to migrate than concave-concave one (0.27%,1/369).Conclusion Difference in operative skills,cage shape,number of fused segments,adjacent endplate shape,and lumbar spondylolisthesis might be risk factors for cage migration after TLIF.
8.Effects of hyperglycemia on proliferation,secretion function and expression of endoplasmic reticulum stress related molecules of pancreatic ? cell line
hai-yan, ZHAO ; lei, QIAN ; xue-lian, FU ; fan, LIN ; hong-li, ZHANG ; xiao, WANG ; guo, LI ; min, LUO
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(05):-
Objective To investigate the effects of chronic hyperglycemia on the proliferation,secretion function and expression of endoplasmic reticulum stress(ERS) related molecules in pancreatic ? cell line MIN6. Methods MIN6 cells were treated with different concentrations of glucose(5.6,25 and 33.3mmol/L) and were harvested at indicated time(24h and 96h) for examinations.Cell proliferation was tested using CCK-8 solution,insulin and proinsulin secretion function was determined by ELISA,and mRNA expression of ERS related molecules(Total XBP-1,Spliced XBP-1) and protein expression of phosphorylated IRE1? were detected by Real-time PCR and Western blotting,respectively. Results After treatment with hyperglycemia for 96h, cell proliferation was significantly lower than that treated for 24h(P
9.Clinical examination and evaluation of stereoacuity with multi-dimensional spacial perception model in children with strabismus and amblyopia
Wen, LIU ; Gang, YU ; Qian, WU ; Wen-hong, CAO ; Yun-wei, FAN ; Qi, LIN ; Hui-hui, CHU ; Ru, ZHANG
Chinese Journal of Experimental Ophthalmology 2012;30(9):806-810
Background There is multi-dimensional order of spatial stereopsis perception in human,however,current stereopsis examination is zero-order of position disparity.A multi-dimensional space perception model is very important for the detection of stereoacuity.Objective This study was to screen the deficit of zero-order,first-order,second-order multi-dimensional spatial stereopsis perception in amblyopia and strabismus children and to explore the association of zero-order,first-order,second-order spatial perception deficit.Methods Multidimensional spacial perception was examined in 79 children aged 4-14 years in Beijing Children' s Hospital.Nineteen normal children,19 children with ametropia amblyopia,12 children with anisometropic amblyopia,18 children with strabismus and 11 children with strabismus combined amblyopia were included this study.The random-dot and line spatial stereopsis perception in zero-order,first-order and second-order were examined with a new system of multidimensional space perception screening.Written informed consent was obtained from each subject or custodian before any ocular examination associated with this study.Results Absence of zero-order,first-order,second-order random-dot channel was found in 24 children (24/79,30.4%),18 children (18/79,22.8%) and 24 children (24/79,30.4%) respectively,with an average percentage of 27.9%.Absence of zero-order,first-order,second-order line channel was examined in 37 children (37/79,46.8%),37 children (37/79,46.8%),32 children (32/79,40.5%),with an average percentage of 44.7%.In the children with a deficiency of the zero-order spatial perception,the children who still remained the first-order or/and second-order spatial perception of random-dot accounted for 41.6% and that of lines accounted for 43.2%.In children without deficiency of zero-order random-dot or lines spatial space perception,deficiency of first-order and/or second-order spatial perception was in 37.5% children.Various order spatial perception deficiency was seen in children suffering from amblyopia or strabismus compared with normal group(P < 0.05).Conclusions There exists spatial perception deficiency in children with amblyopia or strabismus.The patients with zero-order spatial perception absence partially remain a first-order or/and second-order spatial perception;while the patients with normal zero-order spatial perception might have first-order or second-order spatial perception deficiency.The multi-dimensional space perception model has a directive role for the training of visual information process and the treatment of spatial perceptual learning in children with amblyopia or strabismus.
10.Correlation between aquaporins mRNA expression and renal parenchyma thickness in congenital hydronephrotic kidney in children
Jianguo WEN ; Zhenzhen LI ; Yingzhong FAN ; Qian ZHANG ; Hong ZHANG ; Guoxian ZHANG ; Baohong YUE ; Fucheng HE ; Jiaxiang WANG
Chinese Journal of Urology 2009;30(4):234-237
Objective To investigate the relationship between aquaporin-1, -2, -3, -4 mRNA (AQP1-4) and renal parenchyma thickness in congenital hydronephrotic kidney in children. Methods The expressions of aquaporin 1, -2, -3, and -4 mRNA in hydronephrotic kidney of 37 children (aged 60.3±48.8 months) were evaluated with congenital hydronephrosis and control kidney of 6 children (aged 62.7±17.1 months) by using semi-quantitative reverse transcriptase polymerase chain reaction technique. Hydronephrotic kidney parenchyma thickness was measured by B-Ultrasound preoperative-ly and verified at operation. The relations of aquaporin 1, -2, -3, and -4 mRNA to the hydronephrotic kidney parenchyma thickness were analyzed by correlation analysis. Results The aquaporin 1 ,-2,-3, and -4/beta-actin ratio in the hydronephrotic kidney and normal kidney were 0.39±0.22 vs 0.90± 0.10, 0.42±0.20 vs 0.92±0.09, 0.525±0.22 vs 0.98±0.12, 0.30±0.18 vs 0.74±0.21 respec-tively, and the differences were significant (P<0.01). Hydronephrotic kidney parenchyma thickness measured by D-Ultrasound was 5.01±2.38 mm, which was identical with those measured at opera-tion. Significant correlation was found between the levels of aquaporin 1,-2,-3, and -4 mRNA and hydronephrotic kidney parenchyma thickness (r=0.773, 0.772, 0.557, 0.625, respectively; P< 0.01). Conclusions Significant correlation exists between decreased expressions of aquaporin 1 ,-2, -3, and -4 mRNA and atrophic change of renal parenchyma. This result may provide evidence to ex-plain the mechanism why the thinner renal parenchyma thickness, the weaker renal concentration and dilution function.