The concentrations of lactoferrin (LF) in colostrum during the first 8 days and mature milk during the first 4 months of postpartum were determined by electroimmunodiffusion. Meanwile a sensitive enzyme linked imm-unosorbent assay for measurements of LF in neonatal faeces were established. The results showed that human milk, especially colostrum, was rich in LF. The colostrum contained 6.88 ? 3.10mg/ml (M?SD) aad mature milk 3.18 ? 0.37mg/ml LF. LF in human milk was the highest during the first 3 days of lactation with a decline in concentration as lactation proceeds although a wide variation in concentration was noted among different individuals.LF in neonatal faeces showed significant difference in different feeding manners. LF level in the breast-fed infants was 19 times higher than that in the bottle-fed infants. The LF percentage detected in the former was 100% and the latter only 60%. The dynamic variations of LF in faeces of breast-fed infants during the first day of life were found to be relative to LF level in mother'milk.These data demonstrated the influence of high LF level in mother'milk on the concentrations of LF in breast-fed infants' intestine.Furthermore a few amounts of LF was found in meconium and its percentage detected was 35%. This suggested that the production of endogenous LF began from foetus period, but was limited during the first day of life. LF ingested by the breast-fed infants were not completely destroyed and thus may supplement the insufficiency of the endogenous LF in the newborn infants' intestinal tract.

OBJECTIVE:To develop a RAM(Restricted-access media)-HPLC for the determination of plasma concentration of carbamazepine,and to compare the determination results with those determined by FPIA method. METHODS: The plasma samples were centrifugalized,then the venous plasma samples were determined by FPIA and RAM-HPLC,respectively,while the fingertip plasma sample was determined by RAM-HPLC alone. The chromatographic column was Hisep shielded hydrophobic phase analytical column,and the mobile phase consisted of ammonium acetate buffer-acetonitrile (88∶12) at a flow rate of 1.5 mL?min-1. The detective wavelength was set at 280 nm;the sample size was 20 ?L and the column temperature was kept at room temperature. RESULTS: A good correlation was noted between the carbamazepine concentration in venous plasma sample determined by FPIA and carbamazepine concentrations in either venous or fingertip plasma determined by RAM-HPLC,with the correlation coefficients being 0.989 and 0.995,respectively,but the differences were significant(P0.05). CONCLUSION: Both RAM-HPLC and FPIA are applicable for the determination of carbamazepine concentration,but FPIA is more suitable for routine monitoring while RAM-HPLC is more suitable for the related research and monitoring of specific cases. Both methods shows good reproducibility and in which the samples don't have to be pretreated.

Adult ; Death, Sudden ; Humans ; Male ; Middle Aged ; Trichloroethylene ; poisoning

Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Sciatica ; therapy

Immune thrombocytopenia (ITP) is a common bleeding disease in children.Recent advances has indicated that such factors as decreased platelet,fear of bleeding,prognosis,therapy associated side effect and restricted activities,usually decreased quality of life of patients and their parents.This review focuses on domestic and overseas researches on health-related quality of life (HRQoL) in children with ITP,in order to attract more scholars' attention.HRQoL should be recommended as an important indicator in treatment and management of ITP.

Recent research results suggest the existence of cancer stem cell (CSC), a subpopulation of cells with tumorigenic potential that is present in tumor. It has been reported that the occurrence of some tumors is closely related to mesenchymal stem cell as origin of stroma cell. This review discuss the relationship among mesenchymal stem cell, cancer stem cell and cancer.

Artificial ureter has been studied for nearly 60 years, however it is still in experimental stage. So far there are no commercially available products for clinical use except autologous intestines loop even though many progresses have been made. Each approach for making artificial ureter has its own inherent advantages, disadvantages and limitations. But some approaches have shown great potential to be used as ureteral substitutes. Preventing the conduit from salt depositing and ensuring a watertight anastomoses with native tissues are the key factors in designing and manufacturing the prosthetic ureter. Along with scientific and technical improvement, artificial ureter will be successfully used in clinical therapeuticsin in the very near future.

Through the questionnaire survey of community residents,the paper studies the factors influencing users' acquisition of health information from libraries in medical universities and colleges and provides reference and suggestions for socialized services of these libraries.As indicated by the results,the influencing factors include the distance,motivation,environment,limitation and time.On this basis,it proposes solutions,including the improvement of the service mechanism,the utilization of advantages of medical library resources and the establishment of the service system for community residents centering on medical libraries.

Objective:To compare the feasibility,complications and advantages of the nose-intestine tube and the jejunostic tube in early enteral nutrition after double incisions for the therapy of esophageal cancer.Methods: 52 postoprative patients were divided into three groups.The nose-intestine tube group(A group,n =16) and the jejunostic tube group(B group,n = 21) received the enteral nutrition(Nutrison Fibre) and the conrtrol group(C group,n = 15) received intravenous isotonic glucose solution and oral liquid diet after the bowel movement recovery.The blood glucose,the function of liver and kidney,electrolytes and nutritional status were observed.The recovery of bowel movement and other digestive symptoms such as abdominal pain,distention,diarrhea,nausea and vomiting were observed during the period of study.Some complications such as acute intestinal obstruction,pnumonia and rhinolaryngitis were observed during the period of study.Results: The time for placing the jejunostic tube was faster than that for placing the nose-intestine tube(P