Osteoblasts (OB) isolated from newborn SD rats were cultured in vitro.After treatment with different concentrations of 17?-estradiol (10~(-11)-10~(-6)mol/L),the mRNA expressions of osteoprotegerin (OPG) and osteoclast differentiation factor (ODF) in OB were measured by RT-PCR.17?-estradiol increased the expression of OPG in OB with the maximal effect at the concentration of 10~(-8) mol/L.No significant difference was observed in the expression of ODF in OB with different concentrations of 17?-estradiol.The therapeutic effect of estrogen on osteoporosis appears to be related to the enhanced OPG expression in OB at physiological concentration of estrogen.

Objective To observe the expressions of nuclear factor-?B(NF-?B)in brain tissue of rats with repeated febrile seizures(FS)and explore its significance in brain injury of rats with FS.Methods Fifty-one male SD rats were divided into 3 groups according to random number method:normal group(n=14),hyperthermic group(n=19),FS group(n=18).FS models were induced by placing rats in warm bath;the rats without FS after warm-bath were assigned as hyperthermic group ;the normal controls received no treatment.The expression of NF-?B was measured by immunohistochemistry.The apoptosis of brain tissue was determined by terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling(TUNEL).SPSS 13.0 software was applied for statistical treatment.Results In FS group,the number of the NF-?B positive neuron increased much more than that of hyperthermic group and normal group(Pa

Objective To explore the change of somatostatin(SS)in brain areas of juvenil rats with repeated febrile seizures(FSs)and hyparthermia.Methods Fifty-one male SD rats were divided randomly into 3 groups:normal group(NC group,n=14),hyperthermic group(HC group,n=19)and febrile seizure group(FS group,n=18).FS were induced by placing rats in a bath of water.The expressions of SS in DG,CA3,CA1 and CTL were measured by immunohistochemistry.The level of SS in hippocamps of these rats were determined by radioimmunoassay.Results The results of immunohistochemistry shew in HC group,the number of the SS positive neuron were increased in DG(21.10?2.51),CA3(10.60?2.41)in FS group,which was less than that in NC group(10.50?2.12,6.90?2.02),there was no difference in CA1 and CTL.The result of Radioimmunoassay:the level of SS in hippocampus in HC group[(53.74?7.56)ng/g]was higher than that in FS group[(39.54?7.74)ng/g](P0.05).Conclusion There is different change of somatostatin content in some brain areas of rats with repeated febrile seizure,which suggest that SS can increase the affectivity of seizure and promote the seizure.

Objective To carry out a systematic study on the chemical constituents in the fruits of Momordica grosvenori. Methods To isolate pure compounds by using repeated column chromatography, while the structure of a new compound was determined by detailed spectral analysis. Results Four cucurbitane triterpenoid glycosides, mogroside Ⅱ_E(Ⅰ), mogroside Ⅲ(Ⅱ), grosmomoside Ⅰ (Ⅲ), and mogroside Ⅴ (Ⅳ) were isolated from the 50% ethanolic extract of the fruits of M. grosvenori. Conclusion Grosmomoside Ⅰ is a new compound identified as mogrol-3-O-?-D-glucopyranoside-24-O-{[?-D-glucopyranosyl(2-1)]-[?-D-glucopyranosyl (6-1)]-?-D-galactopyranoside} and the other three compounds are known compounds.

Objective To explore the changes of brain-derived neurotrophic factor(BDNF) after repeated febrile seizures (FS).Methods Fifty-one male SD rats were divided into 3 groups randomly: normal group (NC group,n=14) ,hyperthermic group (HC group,n=19),FS group(n=18). FS were induced by hot water bath.The level of BDNF in hippocampus homogenate were measured by enzyme linked immunosorbent assay (ELISA) and the expression of BDNF were measured by immunohistochemistry.The apoptosis of the brain cells were determined by terminal deoxynucleotide mediated nick end labeling (TUNEL).The results were analysed with the software of SPSS 13.0.Results The level of BDNF in hippocampus in FS group(89.90?12.51) ng/g was significantly higher than that in NC group(54.43?18.92) ng/g and HC group(64.09?15.03) ng/g (Pa

Objective:To explore the effect of nontypeable Haemophilus influenzaen(NTHi)strain ATCC49247on pri-mary rabbit tracheal epithelial(TE)cells.Methods:TE cells were isolated with low temperature protease digestion and cul-tured on collagen gel-coated membranes at an air-liquid interface in serum-free medium.Under these conditions,TE cells were proliferated and differentiated into a pseudostratified mucociliary epithelium,which were infected by NTHi.Morphologic changes of the cells were examined by scanning electron microscopy(SEM)and transmission electron microscopy(TEM)after 24h.Results:SEM showed that bacteria adhered to non-ciliated cells;death or apoptosis occurred in90%of TE cells and cil-iaries were broken.TEM showed NTHi adhered to the cell surface on which there were many microvillus.Lamellipodia and microvilli surrounded bacteria within vacuoles of airway cells.Conclusion:NTHi can attach to non-ciliated cells,the latter de-vours the bacteria by lamellipodia and microvilli.NTHi is toxic to TE cells,resulting in the death or apoptosis of TE cells.

Cell immobilization is a new biotechnology The definition, classification, and carrier selection of cell immobilization are presented in details The technique is efficiently applied to treating strength organic wastewater, nutrient and heavy metals removal of wastewater, as well as hardly biodegradated wastewater It has a widely applied prospect in wastewater treatment

Based on the social background of increasingly serious aging of the population and the growth of chronic diseases,the paper introduces the mode of Connected Health (cHealth),analyzes the differences between cHealth and Mobile Health (mHealth) as well as the advantages of cHealth,introduces the application of cHealth in contemporary society,and discusses the challenges of cHealth,including the optimization of sensing strategy,creation of data integration,analysis on the new mode,optimization of feedback strategy and development of new health insurance modes.

OBJECTIVE: To investigate whether the allergic factors impact the severity of chronic rhinosinusitis or not, further more, to explore the relationship between allergic rhinitis and chronic rhinosinusitis. METHOD: A retrospective review was done on 103 patients. All of these patients were under functional intranasal endoscopic sinus surgery after expectant treatment is ineffective. We devided the patients into different groups according to the result of skin prick and specific IgE and if there is difference in VAS score, Lund and Kennedy endoscopic score, Lund-Mackay CT score between the groups. We also analysed the symptoms in different chronic rhinosinusitis patients allerged to variant kinds of allergen. The SPSS 17.0 software was used to analyze the data. Statistical analysis was performed by t-test, rank order test or χ2 test. RESULT: The duration of the disease, VAS score of nasal blockage, score of Lund-Mackay CT and Lund and Kennedy endoscopic before the operation were in no statistical sense after when compared with the two groups of patients with chronic rhiriosinusitis who grouped according the result of skin prick and specific IgE. The VAS score of facial pressure and loss of smell was higher in patients with chronic rhinosinusitis which the skin prick and specific IgE were positive. The VAS score of nasal discharge was higher in patients with chronic rhinosinusitis who got negative skin prick and specific IgE result. The symptoms of chronic rhinosinusitis improved with operation no matter the group of skin prick and specific IgE positive or negative and VAS score of nasal blockage improved significantly in negative group. The symptoms of sneezing, rhinorrhoea and rhinocnesmus improved after operation among the chronic rhinosinusitis patients with skin prick and specific IgE. The number of cockroach allergy is larger among the patients with chronic rhinosinusitis without polyps than the one among the patients with chronic rhinosinusitis with polyps. CONCLUSION Allergic factor didn't impact much the severity of chronic rhinosinusitis patients who were failed in expectant treatment, besidesthe postoperative outcomes showed that only influence the severity of nasal discharge, facial pressure and loss of smell. Different kinds of allergen were found between the patients of chronic rhinosinusitis with or without polyps. No significantly functional endoscopic sinusitis sugrery outcome were related to the allergic factor. But the allergic factor may interfere the remission of nasal discharge after surgery.
Administration, Intranasal ; Allergens ; Chronic Disease ; Endoscopy ; Humans ; Nasal Obstruction ; Nasal Polyps ; surgery ; Polyps ; Postoperative Period ; Retrospective Studies ; Rhinitis ; immunology ; surgery ; Rhinitis, Allergic ; surgery ; Sinusitis

Objective To improve the utilization ratio of operating room in Affiliated Hospital of Central South Uni-versity Xiangya Medical School by providing more comprehensive reference data for the design of surgical time pre-diction and dispatch system. Methods A questionnaire was designed according to the review of literature and consul-tation of experts for investigating the surgical time influencing factors. The surgical time influencing factors were ana-lyzed by stratified sampling. Results The surgeons-related factor was the highest influencing factor while the pa-tients themselves-related factor was the lowest influencing factor in the 2-dimensional factors. The selected 38 1-dimensional factors could affect the operating time with their mean influencing value>2 . 45 . The recognition of sur-gical time influencing factors was different in different operating rooms. Conclusion There are a variety of surgical time influencing factors. However, the surgeons-related factor is the highest influencing factor. The cognition of anesthesia-related factors and surgeons-related factors differs in different operating rooms.