Objective To optimize and verify an in vitro detection method for bioactivity of recombinant human bone morphogenetic protein-2(rhBMP-2)based on ASTM F2131-02 standard test.Methods Based on the method established by the American Society for Testing Material(ASTM),the detection cell lines(W-20-17 and C2C12 cells),dilution mode of rhBMP-2(2 and 3 times serial dilution),culture time(24,36,48 and 72 h),and freezing temperature(-20 and-80 ℃)were optimized,and the specificity,intermediate precision,linearity and durability of the optimized method were verified. The specific activity of rhBMP-2 from different manufacturers was detected using the optimized method.Results C2C12 cells were determined as detection cells;The best dilution method of rhBMP-2 was 2 times series dilution;The optimum cell culture timewas 48handtheoptimum freezingtemperatureofcellswas-80℃. C2C12 cells responded specifically to rhBMP-2 induction when adding heteroprotein(bovine serum albumin)to rhBMP-2;The CV of 12 detections by 2 experimenters at 2 time points was11. 7%. The theoretical titer of rhBMP-2 showed a good linear relationship with the measured titer in the range of 70%-142%,and the fitting linear regression equation was:y = 0. 983 1 x-0. 010 3,R~2= 0. 996 4;The CV of the detected specific of rhBMP-2 using 4,11 and 21 passages of C2C12 cells was 5. 2%. The CV of specific activity of rhBMP-2 samples from different manufacturers detected by the optimized method repeatedly for two times was 1. 3%-7. 5%.Conclusion The optimized method showed good specificity,intermediate precision,linearity and durability,and might be used to detect the bioactivity of rhBMP-2 in vitro,which provided a reliable analytical method for its research,production and application.

The first step of bacilli culture is inoculation bacteria on culture medium. Designing a device to increase efficiency of inoculation is significative. The new device is controlled by SCM. The stepper motor can drive the culture medium rotating, accelerating, decelerating, overturn and suspending. The device is high practicability and efficient, let inoculation easy for operator.
Bacteriological Techniques ; instrumentation ; Equipment Design ; Software Design

At present, selective 5-hydroxytryptamine (5-HT) reuptake inhibitors (SSRIs) and 5-HT and noradrenaline (NA) re-uptake inhibitors ( SNaRI) are the first choice for the clinical treatment of depression, however, the clinical adverse effects of the drugs have become a prominent problem affecting the clinical efficacy. Chinese medicines have some experience in the treatment of depres-sion, and the combined use with the above antidepressants can enhance the efficacy and / or reduce the adverse effects. The article re-viewed the status of the combination of SSRIs and Chinese medicines in order to provide reference for the clinical treatment of depres-sion.

Intraperitoneal. injection of the essential oils of Asarum himalaicum and A. heterotropides var. mand shuricum can inhibit the proliferation of granuloma induced by implantation of cotton pellets in rats, and change the distribution of Copper and Zinc,the two necessary trace elements for rats,in rat serum and liver.They reduce concentration of Zinc but increase that of Copper in the serum,while elevate the concentration of Zinc but reduce that of Copper in liver. The oil of A. himalaicum collected from Ningxia Region showed even stronger effects.

Ceramide (Cer),as the center molecular of sphingolipids metabolism,functions as a bioactive mediator of different cellular processes,such as cell growth,differentiation,senescence and apoptosis,besides being structural component of cellular membrane.It can inhibit cell proliferation and promote cell apoptosis via multiple signal pathawys.Chemicals anti-cancer properties based on Cer metabolism that upregulate its level are confirmed in plenty of experimental researches,and parts of research productions are applied in anticancer clinic experiments.

A lot of progresses in understanding of the production and effects of NO have been made in recent years. Large number of studies showed that NO play an important role in the pathogenesis of septic shock. Some other investigators reported that NOS inhibitors could have the potential therapeutic value in septic shock treatment.

[Objective] Have a research on therapeutic methods of phlegm syndrome of Li Ziyi in Qing dynasty, to sum up his therapeutic experience of such syndrome.[Methods]Analysis on the related states and cases on the base of researches on the contents of “Approaches of phlegmatic scrofula ”which was written by Mr. Li, elaborating his characteristics in treating such syndrome. [Results] Mr. Li considered that phlegm syndrome can be classified into milder phlegm and scrofula according to its level of the patient ’s condition and therapeutic effect. He also believed that several aspects should be taken into consideration through the therapeutic process:treat by internal combing with external treatment ,pay attention to seven emotions and smoothen the temper, emphasize nursing care and obey to the contraindication strictly. [Conclusion]There are features in Mr. Li ’s view on phlegm and his treating method of phlegm syndrome. His treating method has a good therapeutic effect and it is worth popularizing.

Adolescent ; Asthma ; blood ; Bronchitis ; blood ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Male ; Uteroglobin ; blood

Objective:To explore the influencing factors of communication between pediatric junior nurses and parents of hospitalized children,and to provide basis for further improving the communication ability of junior nurses.Methods:A semi-structured interview was conducted among 10 pediatric junior nurses. Colaizzi phenomenological seven-step analysis method was used to analyze the data.NVivo10.0 software was used to manage,excavate and search text data.Results:Four themes were extracted,including self-factors,environmental factors,lack relevant training and lack of human resources.Conclusions:There are many influencing factors in the communication between nurses and parents of hospitalized children.Managers should pay attention to them by targeted training and improving communication environment, the communication ability of junior nurses can be improved,so as to improve the quality of nursing and family satisfaction.

Dopa preparations are currently first choice for the clinical treatment of Parkinson' s disease. With the application time extension and the dose increase of dopa preparations, the therapeutic effect is smaller and smaller, while the side effects are more and more significant, which have become the main problems affecting their clinical application. The current researches showed that the combination of traditional Chinese medicines and dopa preparations can improve the efficacy, decrease the dose of western medicines, reduce the adverse effects and so on. The paper reviewed the progress in the combination of dopa preparations and traditional Chinese medicines in the treatment of Parkinson' s disease in recent ten years.