Objective:To use TDP therapy combined with special care and treatment of cesarean section wound, clinical observation. Methods:The therapy combined with psychological, pain, health education and other special nursing treatment with TDP for 38 cases of the observation group of patients, the patients in control group took traditional postoperative nursing. Results:in the observation group were the wound infection rate was 7.9%, the incidence of complications was 18.4%, the control group were 28.9%, 42.1%, And the observation group at the end of the third week wound shrinking rate was 38.62±26.31, control group was 27.14±12.42, the observation group at the end of the 1 week, 2 week, 3 week pain levels were 4.68±1.24, 4.14±1.21, 3.96±1.02, comparing the two groups of patients, the wound infection rate reduced rate and pain level, (x2=4.2903, x2=4.2113;P<0.05),the difference has statistical significance. Conclusion:combined with special care, the postoperative parturient with TDP therapy effect is good, is worth the clinical promotion.

Objective:This study aimed to investigate the effects and the molecular mechanism of JNJ-7706621 on cell cycle and apoptosis of breast cancer cell lines. Methods:Breast cancer cell lines T47D and MDA-MB-231 were cultured in vitro and treated with JNJ-7706621 at varying concentrations. MTT assay was used to measure cell proliferation. Flow cytometry was applied to analyze the distribution of cell cycle and apoptotic rates. Western blot was performed to analyze the expression of cyclin B1-CDK, the phosphoryla-tion levels of CDK1Thr161 and CDK1Tyr15, as well as the expression of p53, Bcl-2, caspase3 and poly(ADP-ribose) polymerase (PARP). Results:JNJ-7706621 inhibited the proliferation of the T47D and MDA-MB-231 cells in a dose-and time-dependent manner. Flow cytometry showed that the T47D and the MDA-MB-231 cells in the G2/M-phase significantly increased after treatment at varying concentrations (0, 1, 2, 4μM) of JNJ-7706621:the G2/M-phase rates at the corresponding concentrations were (12.66±1.55)%, (20.63± 1.32)%, (23.20±1.82)%, and (32.19±2.37)%, respectively, for the T47D cells (P<0.05), and the G2/M-phase rates were (16.22±1.48)%, (21.45±0.85)%, (25.25±1.26)%, and (31.08±1.16)%, respectively, for the MDA-MB-231 cells (P<0.05). The rates of apoptotic cells were also significantly increased (P<0.05). Western blot results indicated that JNJ-7706621 exerted a slight effect on the expression of CDK1 and p53 and that the phosphorylation level of CDK1 decreased at the Thr161 site but increased at the Tyr15 site. In addition, cleaved caspase3 and PARP increased, whereas Bcl-2 and cyclinB1 decreased significantly. Conclusion:JNJ-7706621 can significantly inhibit the proliferation of breast cancer cell lines T47D and MDA-MB-231 by inducing cell cycle arrest and apoptosis, which may be associated with the downregulation of the CDK1 phosphorylation at the Thr161 site.

[Purpose] For almost ten years of traditional Chinese medicine treatment of infantile herpetic stomatitis, shows that its treatment efficacy and safe-ty, and points out the advantages and disadvantages of traditional Chinese medicine treatment of the disease; in traditional Chinese medicine for infantile herpetic stomatitis targeted multi-channel treatment and flexibility, to open up broader prospects. [Method] To col ect reference infantile herpetic stomati-tis, traditional Chinese medicine treatment of relevant literature reports, the etiology and pathogenesis, dialectical classification and treatment of traditional Chinese medicine, including herbal medicinal broth internal medicine, proprietary Chinese medicine oral treatment, relevant experience and proprietary Chinese medicine atomization, external treatment, acupuncture, massage and so on, carry on the induction, summary. [Results] Chinese medicine treatment for infantile herpetic stomatitis, high total effective rate in clinical effect is better, and with less side effects. [Conclusion] Traditional Chinese medicine in the clinical research of the disease in recent years shows certain advantages, at the same time, carries out multicenter, large sample, prospective clinical study, objective evaluation the clinical curative effect of traditional Chinese medicine treatment of the disease; a clear indication of traditional Chinese medicine treatment of the disease, targeted research and development of convenient and effective inside governance and outside governance drugs wil be the re-search direction of treatment of this disease.

Objective To investigate the therapeutic effect of flupentixol and melitracen tablets for irritable bowel syndrome (IBS).Methods Relevant concerned with the therapeutic effect of Flupentixol and melitracen tablets and routine medicine were reviewed by China national knowledge infrastructure.All reviewed literatures were accessed by Jadad quality scale.Statistic data was analysed by RevMan5.0 and Stata.Results Twelve articles of randomized controlled trial were collected.Among these study,there were 1384 patients,which divided into treatment group (n =704) and control group (n =680).The results of heterogeneity showed that x2 =25.48,P =0.008,I2 =57％.Therefore the random effects model was selected.The combined relative risk is 1.39 and the 95％ CI was 1.27-1.53.Conclusion Compared with traditional treatment,flupentixol and melitracen tablets can obviously improve the therapeutic effect of IBS.

Pim-3,a new member of the the proto-oncogene pim family which expresses serine and threonine kinase activity,shows a high similar to pim-1 and pim-2 at the function,structure and sequence.Pim-3 plays an important regulative role in the cell cycle transform,cell apoptosis and occurrence of the malignant tumors by phosphorylating numerous specific substrates.

Objective　To determine the effect of posterior vitreous detachment on the prognosis of branch retinal vein occlusion　(BRVO).　Methods　One hundred and sixteen patients (116 eyes) with BRVO who underwent vitreous examination were retrospectively studied.The relationship of vitreous conditions to posterior segment neovascularization and macular edema was statistically investigated.　Results　In 40 ischemic cases,12 of 25 eyes (48.0%) with no posterior vitreous detachment (PVD) or partial PVD developed retinal or optic disc neovascularization ,or both,but only one of the 15 eyes (6.7%) with complete PVD developed neovascularization during a mean follow-up period of 10.7±2.2 months　(P＜0.05).Diffuse macular edema was found in 45 eyes　(38.8%).The incidence of macular edema was significantly higher in eyes with vitreomacular attachment　(51.5%)　than in those with vitreomacular separation　(22.0%)(P＜0.01).　Conclusion　It was suggested that complete PVD may play a role in protecting eyes with BRVO from posterior segment neovascularization and macular edema.

Objective To investigate the effects of dexmedetomidine on tonsillectomy in children with sevoflurane combined with anesthesia recovery period of restless.Methods A total of 62 patients with tonsillectomy under general anesthesia from 2011 to 2015 in our hospital werecollected and randomly divided into the control group and the experiment group with 31 cases in each.Two groups of children were treated by 7％sevoflurane inhalation anesthesia induction,supplemented by a small amount of intravenous anesthetics propofol 2 mg/kg,sufentanil 2 μg/kg,CIS atracurium 150 μg/kg intravenous injection.Patients in the experiment group were treated by dexmedetomidine 0.2 μg/(kg·h)continuous intravenous infusion until the end of the operation,patients in the control group were treated by normal saline intravenous infusion.Compared the systolic blood pressure and heart rate of the 2 groups of before anesthesia induction,after anesthesia induction,after operation 5min,the end of the operation ; occurred rate of restless at after anesthesia extubation 5minutes,10minutes,15minutes,20minutes,25minutes.Results Compared with the control group,the systolic blood pressure and heart rate of the experiment group without statistical significance before anesthesia induction; after anesthesia induction,after operation,5min,at the end of operation,the systolic blood pressure and heart rate were lower(P<0.05); children recovery time,extubation time,recovery time of delivery from the anesthesia room were shorter(P<0.05); occurred rate of restless were lower after anesthesia extubation 5minutes,10minutes,15minutes,20minutes,25minutes(P<0.05).Conclusion Dexmedetomidine can prevent tonsillectomy after sevoflurane anesthesia result in the recovery period of restless.

Cellular senescence is a permanent cell cycle arrest that may prevent the aged or abnormal cells from further expansion and is therefore a safeguard mechanism against unlimited cell proliferation.Recent studies demonstrated that oncogene-induced senescence could prevent neoplastic transformation in vitro and in vivo,and oncogene might cause tumors only in senescence deficient cells.Although the mechanism of the cellular senescence has not been completely illustrated,activation of P16INK4a-RB and ARF-P53 pathways has been implicated in this process.Further investigation of the signaling pathways leading to senescence may offer new avenues for tumor treatment.

It is of great importance that deep learning of computer for the automate identification of the two-dimensional image of Chinese herbal slices is valuable in the application to medicine,production and education.Traditional methods usually extract low-level image features for the identification,but they cannot give robust recognition results under complex backgrounds.Therefore,higher level image representation is necessary in the image identification.A public Chinese herbal medicine database was constructed with 50 common categories and 2,554 images in total,for training and evaluating our recognition model.Then,the sofimax loss function was adopted to train the convolutional neural network model.As a result,the convolutional neural network can achieve the average precision of 70％ under all the 50 medicine herbal classes.In conclusion,convolutional neural network can obtain good results in image identification with complex backgrounds and mutually occluded herbal slices,which has promising potential for future applications.

Objective To investigate the effects of caffeine on cerebral cortical activity in preterm infants. Methods The preterm infants with 28-34 week gestational age and without asphyxia at birth were recruited as the subjects from January to September 2016. The infants who received conventional caffeine citrate were assigned to the caffeine group, while the infants with the same postmenstrual age did not receive any drugs that excite respiratory center were assigned to the control group. In the caffeine group, after the use of caffeine citrate stopped, the amplitude integration electroencephalogram (aEEG) recording were performed at 33, 34, 35 weeks of postmenstrual age. All the aEEG recordings were continuously monitored for at least 4 hours. The aEEG images were assessed by Burdjalov scoring system, and the interburst intervals were calculated. The effects of caffeine on preterm infants' cerebral cortical activity in early life were analyzed. Results In a total of 49 preterm infants recruited, 21 were in caffeine group and 28 in control group, and the ratio of male and female was 1.45:1. At the same postmenstrual age, the total score and individual scores of aEEG assessed by Burdjalov scoring system were not significantly different between caffeine group and control group. However, the length of interburst interval was significantly shorter in caffeine group than that in control group (P<0.05). All the recruited preterm infants had no seizure-like activity when the aEEG was monitored. Conclusion The use of caffeine citrate has effects on EEG activity in early life of preterm infants, which may accelerate the brain maturation of preterm infants