OBJECTIVE: To summarize the cervical dermatoglyphics distribution in 229 participants, and to design dermatoglyphic incision for cervical lymph nodes dissection, including II , III, IV or/and V regions, in accordance with the requirement for cosmetology. METHOD: To keep the patient's head right forward when the number and locations of the dermatoglyphy were collected by macroscopic observation. Upper Cricoid-Region is defined as the region above the cricoids, and Cricoids-Collarbone-Region is referred to the region below the cricoids. The relationship among the distribution of cervical dermatoglyph, patients' age, gender, and body mass index were analyzed. According to the distribution of cervical dermatoglyph, when performing the regional cervical lymph nodes dissection in patients with laryngocarcinoma and thyroid cancer, cervical dermatoglyphy incision or parallel dermatoglyphy incision were designed, and the operation time, operative complications, and cosmetology effects after surgery were observed. RESULT: Distribution of cervical dermatoglyphics was statistically correlated to the age, gender, and body mass index (P < 0.05) of patients. The follow-up time were 12 months to 49 months, 19 months on average. Average operating time of unilateral lymph nodes dissection was (46 +/- 12) minutes. Patients undergone the designs of cervical dermatoglyphy incision or parallel dermatoglyphy incision, suffered neither skinflap necrosis nor accessory nerve injury. One patient had lymphatic fistula after surgery, and relieved by conservative treatment. One with T3 N2 M0 laryngocarcinoma got V region lymph nodes recurrence 6-months after surgery. CONCLUSION The cervical lymph nodes dissection with dermatoglyphy design is safe with prominent cosmetology effects. It will take a little longer time to perform the neck dissection with the dermatoglyphy incision in the beginning.
Adolescent ; Adult ; Aged ; Asian Continental Ancestry Group ; Child ; Child, Preschool ; Dermatoglyphics ; Female ; Humans ; Laryngeal Neoplasms ; surgery ; Male ; Middle Aged ; Neck ; anatomy & histology ; Neck Dissection ; methods ; Young Adult

Objective To explore effect of miR‐302a on proliferation and apoptosis in folate deficiency mouse embryonic stem cell (mESC) .Methods The cases were divided into complete culture medium group (control group) ,folate‐deficient culture medi‐um group (folate‐deficient group) ,folate‐deficient culture medium plus miR‐302a mimic group (miR‐302a group) .The expression of miR‐302a was examed by RT‐PCR in control group and folate‐deficient group .To construct miR‐302a mimic and then was trans‐fected into the folate‐deficient culture medium mESC .Effect of miR‐302a mimic on mESC viability was detected by MTT assay ,the effect of miR‐302a mimic on mESC apoptosis was examed by Annexin V‐FITC/PI flow dual‐staining method ,the effect of miR‐302a mimic on mESC cycle was examed by flow cytometry .The activation of phosphatidylinositol 3 kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) and expression of CyclinD1 ,p21 and p27 was assayed by Western blot . Results The expression of miR‐302a was lower in folate‐deficient group(P<0 .01) .Compared with control group ,the viability of mESC was lower ,the apoptosis of mESC was higher ,the cell cycle was arrested in G1 phase ,the level of phosphorylation of AKT and mTOR was lower ,the expression of CyclinD1 was lower ,the expression of p21 and p27 was higher in folate‐deficient group , with statistical significance (P<0 .01) .Compared with folate‐deficient group ,the viability of mESC was higher ,the apoptosis of mESC was lower ,G1 phase was shortened ,the level of phosphorylation of AKT and mTOR was higher ,the expression of CyclinD1 was higher ,the expression of p21 and p27 was lower in miR‐302a group with statistical significance (P<0 .01) .Conclusion These results suggested miR‐302a exerted anti‐apoptosis and promote cell proliferation in folate‐deficient culture medium ,which might be related to PI3K/AKT/mTOR signal pathway .

As a proto-oncogene, urothelial carcinoma antigen 1 (UCA1) is highly expressed in many human tumors such as bladder cancer, gastric cancer, breast cancer, ovarian cancer and brain glioma, which shows important application value in the diagnosis, treatment and prognosis of tumor.Studies show that UCA1 can promote tumor cell proliferation through multiple signaling pathways and molecular mechanisms, which may become a new potential target for the diagnosis and treatment of tumor.

Malignant tumor is an individual disease that caused by gene mutation and disorder of protein expression.The abnormity of mammalian target of rapamycin (mTOR) signaling pathways is closely related to the tumors,and its main biological function is the regulation of protein synthesis and vascular endothelial cell proliferation.However,the activation of mTOR can increase the expression of hypoxia-inducible factor-1 (HIF-1),maintain the acid microenvironment of tumor,promote tumor cell invasion and metastasis,and affect prognosis of tumor.At present,targeted therapy with mTOR and HIF-1 for new targets and gene therapy have become hot points.

Objective To isolate and identify the photoreceptor precursor cells isolated from newborn mouse retina. Methods Fourteen newborn littermate mice were randomly divided into seven groups (P1-P7,2 each). The retinal cells were isolated from the newborn mice on postnatal 1-7d respectively,and then cultured in vitro. The cellular growth state was observed under inverted phase contrast microscope. After in vitro expansion,5-bromo-2-deoxy-uridine (BrdU),neuroepithelial stem protein (Nestin),neural retina leucine zipper (Nrl) and Opsin in cultured cells were detected by immunohistochemistry and immunofluorescence techniques. Results Most of retinal cells isolated from the seven groups (P1-P7) of newborn mice proliferated and adhered to the plate in the particular medium. Most of the growing cells were BrdU-positive cells. A part of the cells could express neural stem cell specific antigen-Nestin,the positive expression rate of Nestin in the seven groups (P1-P7) were 31.0%,31.6%,32.3%,30.2%,31.2%,30.9% and 29.5%,respectively,with no significant difference. Some growing cells expressed Nrl,and the expression of Nrl was increased significantly on the third day after birth,and then increased in small range,the positive expression rate of Nrl in the seven groups (P1-P7) were 20.6%,35.2%,65.5%,68.6%,71.6%,73.0% and 73.3%,respectively. The positive expression of Opsin was found only in a few cells of P5-P7,while the cells in groups P1-P4 did not express Opsin. Conclusions The photoreceptor precursor cells are presented in neonatal mouse retina. They have the ability of proliferation and may differentiate into retinal photoreceptor cells,having a potentiality of expressing Opsin. The photoreceptor precursor cells are increased significantly on the third day after birth,and then differentiate into mature retinal photoreceptor cells gradually.

Objective To investigate the antioxidant activity of Enteromorpha prolifera by ABTS radical scavenging assay in vitro and provide scientific basis for exploitation of Enteromorpha prolifera.Methods The antioxidant activities of extracts by different solvents from Enteromorpha prolifera and water extracts from different Enteromorpha prolifera samples were investigated by ABTS radical scavenging assay.Moreover,the antioxidant ability of the water extract from Enteromorpha prolifera was evaluated by using caffeic acid and ascorbic acid as positive control standards.Results The water extract from Enteromorpha prolifera provided the highest radical scavenging activity,and the antioxidant capability of 1mL of water extract from Enteromorpha prolifera was equivalent to 1mL of 2.55 10~(-3) mg?mL~(-1) caffeic acid or 1mL of 2.89?10~(-3)mg?mL~(-1) ascorbic acid.However,the extraction methods,dry sample or not and different kinds of Enteromorpha prolifera samples had certain influence on the antioxidant activity of water extract from Enteromorpha prolifera. Conclusion There are abundant antioxidant compounds present in the aqueous extract of Enteromorpha prolifera,and it can be exploited as latent oxidation inhibitor.

Biomarkers, Tumor ; metabolism ; therapeutic use ; Brain Neoplasms ; diagnosis ; metabolism ; therapy ; Glioma ; diagnosis ; metabolism ; therapy ; Humans ; Ki-67 Antigen ; metabolism ; Molecular Targeted Therapy ; Myelin Proteins ; metabolism ; Neoplasm Invasiveness ; Nogo Proteins

Animals ; Cyclooxygenase 2 Inhibitors ; therapeutic use ; Humans ; Mouth Mucosa ; pathology ; Mouth Neoplasms ; drug therapy ; pathology ; prevention & control ; Precancerous Conditions ; drug therapy ; pathology ; prevention & control

Animals ; Brain Neoplasms ; etiology ; metabolism ; pathology ; Glioma ; etiology ; metabolism ; pathology ; Humans ; Neoplastic Stem Cells ; pathology ; physiology ; Signal Transduction ; Stem Cell Research