Objective To explore the most suitable chemostatic condition and a method of cell-counting in reconstituted basement membrane invasion experiment in vitro. Method Reconstituted basement membrane invasion experiment was employed, and conditioned medium, fibronectin (FN), and a combination of conditioned medium and FN were respectively used as chemoattractants was placed in the lower compartment of the chamber. After the filters were stained with hematoxylin and eosin (HE), two kinds of cell-counting methods were employed. One was random cell-counting to count the cells in 5 fields infiltrated in the different areas of the filter membrane under 40?magnification, and the other was whole cell-counting of the infiltrating cells on the whole filter membrane with 4? magnification and image analysis system. Results The number of infiltrating cells in the presence of the chemoattractants was much larger than that of the control group. The number of infiltrating cells of the conditioned medium group was larger than that of FN group. The numbers of infiltrating cells in the combination of conditioned medium and FN groups were significantly larger than those of conditioned medium and FN alone groups. The accuracy of method of random cell-counting under 40? magnification was poorer than that of whole cell-counting with image analysis system. Conclusion Chemoattractants exert strong effect on the tumor cells invasion, and different chemoattractants show different chemotactic abilities. By using image analysis system to count all the infiltrative cells of the whole filter membrane is an objective and accurate cell-counting method.

106 patients with liver fibrosis, 58 men (54.72%) and 48 women (45.28%), were treated with Xinganbao. All of these cases were diagnosed as portal hypertension of advanced schistosomiasis. The drug was given in a daily dose of 2.25g (250mg/capsule) 3 times a day for a succession of 2 months.The result of our study suggests that Xinganbao therapy can relieve and/or eliminate the clinical symptoms of advanced schistosomiasis with liver function recovered, serum albumin raised, ?-globulin decreased, immune function adjusted and caliber of portal and spleen vein much diminished. The total rate of efficacy was 89.62%, of which, 14.15% (15 cases) markedly improved while 75.47% (80 cases) moderately.

Chronic HBV infection may not only cause liver damage , but also lead to renal injury . HBV-related kidney injuries mainly include ( 1 ) HBV-associated glomerulonephritis , ( 2 ) hepatorenal syndrome, and (3) kidney injuries caused by long-term nucleos(t)ide analogues therapy.This paper mainly reviews the pathogenesis and therapeutic principle of HBV-related kidney injuries , so as to provide the foundation for clinical management .

Animals ; Cardiomyopathy, Dilated ; metabolism ; Connexin 43 ; metabolism ; Disease Models, Animal ; Hypericum ; Male ; Plant Extracts ; pharmacology ; Rats ; Rats, Wistar

Objective To establish an ELISA for detecting antibody against Aspergillus fumigatus pectate lyase A(anti-PlyA),and evaluate its diagnostic value for invasive aspergillosis (IA).Methods An indirect ELISA for IgG antibody a-gainst PlyA was established using PlyA as coating antigen.The serum from 97 IA patients,80 non-IA patients and 200 healthy donors were tested,the results were compared with anti-DPPV (antibody against Aspergillus fumigatus dipeptidyl peptidase V fragment)and anti-TR (antibody against Aspergillus fumigatus thioredoxin reductase).Results The intra-as-say coefficients of variation of the ELISA method for detecting anti-PlyA was 5.3%,and inter-assay coefficients of variation was 10.9%.The sensitivity and specificity of diagnosis of IA were 62.9% and 90.4%,respectively.The positive rates of an-ti-PlyA in non-neutropenic and neutropenic IA patients were 43.8% and 72.3%,respectively (χ2 =7.493,P <0.05).There was no significant difference between the positive rates of anti-PlyA (62.9%),anti-TR (60.8%),and anti-DPPV (67.0%) (χ2 =0.562,P > 0.05).When combined anti-PlyA,anti-TR,and anti-DPPV,the diagnostic sensitivity for IA patients in-creased to 92.8%.Conclusion An ELISA for detecting anti-PlyA was successfully established.The diagnostic value of these three kinds of antibody was superior in non-neutropenic IA patientsto that in neutropenic IA patients.The combined detec-tion of three antibodies could provide higher sensitivity.

Objective To compare the effect of three types of lipid emulsions on the IL-1βand IL-6 expressions in acute lung injury induced by lipopolysaccharide (LPS) in rats. Methods One hundred young SD rats were divided into control,LPS,ω-6,ω-9 andω-3 group. Age 29 days, the control group were intravenously injected with saline,ω-6 group injected lipid emu-sion (C14-24),ω-9 group injected long chain fat emusion,ω-3 group injected fish oil fat emusion. Then the control group fol-lowed by intratracheal instillation of saline, and the reamining four groups were sprayed with LPS. Pathologic changes in lung tissue section were observed. The expression levels of IL-1βmRNA and IL-6 mRNA were determined by RT-PCR,and the con-centrations of IL-1βand IL-6 in bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immune-specific assay. Results Infiltration and bleeding were observed in lung tissue under light microscopy in ALI rats. The lung indexes and patho-logical scores of ALI model groups were significantly higher than those of control group (P<0.005). The expressions of IL-1βand IL-6 mRNA in groupsω-9 andω-3 were significantly higher than those in groups LPS andω-6 (P<0.005). The levels of IL-1βand IL-6 in BALF in groupsω-9 andω-3 were significantly higher than those in groups LPS andω-6 (P<0.005). There was no significant difference (P>0.005) of the expressions of IL-1βand IL-6 mRNA betweenω-9 andω-3 group. Conclusions ω-6 PUFAs can accentuate inflammation by up-regulating the levels of IL-1βand IL-6 whileω-9 PUFAs andω-3 PUFAs can relieve inflammation by down-regulating the levels of IL-1βand IL-6.

Objective To study the clinical therapeutic effect of arthroscopic debridement cooperated with injecting chuanxiongqin in treating knee osteoarthritis. Methods Sixty cases of knee osteoarthritis were randomly divided into 2 groups: treating group and control group, 30 cases in each group. The treating group were treated by arthroscopic debridement cooperated with injecting chuanxiongqin;the control group were given hyaluronate-Na after received arthroscopic debridement. The operation evaluation criteria of JOA was taken to value the therapeutic effect at 1 week, 3 months, 6 months and 12 months postoperation. At the same time, nitric oxide (NO) content in joint fluid were measured at preoperation, 1 week, 4 weeks, 3 months postoperation. Results At 1 week postoperation, there were no statistically significant differenced > 0.05) between the two groups,at 3, 6 and 12 months postoperation, the therapeutic effect of the treating group were much better than those of the control group (P < 0.01);NO content in joint fluid of preoperative treatment group [(90.32 ± 22.24) μmol/L]and control group [(90.31 ±22.20) μ mol/L]was similar, there was no significant difference (P >0.05). At 3 months postoperation, NO content in joint fluid of the treating group [(85.63 ± 30.63) μ mol/L]decreased significantly compared with preoperative (P < 0.05 ) , and the content was much lower than that of the control group [(89.66 ± 20.63 ) μ mol/L], there was significant difference between the two groups (P < 0.05). Although NO content in joint fluid of the control group was decreased, but the difference was not statistically significant (P >0.05). Conclusions Arthroscopic debridement with injecting chuanxiongqin in treating knee osteoarthritis can control inflammatory reaction of cartilage and synovia]in effect, improve the therapeutic effect. Therefore, it should be avalid method in treating knee osteoarthritis.

Objective To evaluate the factors affecting the treatment of old persons diagnosed hypertension. Method A sample of 55566 persons from the community of Yanji area of Shanghai was recruited. Results Among the 19295 persons over sixty there are 5842 patients with hypertension, the morbidity was 30.3%. Among the 36271 persons aged fifteen to fifty-nine there are 3536 patients with hypertension, the morbidity is 9.7%. The ratio of non-treatment in the old age group is 3.1% and in younger group is 8.8%. However,the compliance to the treatment was better in the younger than in old group(73.7% vs 56.2%). The age,hypertension grade and sex were all accounted for the differences in the regularity of the hypertension treatment. Conclusion Further effort on the education of non-old hypertensive patients is essential to decrease the damage from hypertension.

Objective To investigate the effect of di-( 2-ethylhexyl ) phthalate (DEHP) on pregnant rat and the protection of zinc.Methods Fifty rats were randomized equally into 5 groups consisting of blank (given 1 ml 0.9％ sodium chloride),corn oil (given 1 ml corn oil),zinc (given 1 ml zinc gluconate including 1.2 mg zinc),DEHP (given 50 mg · kg-1 · d-1 DEHP)and DEHP + zinc (given 50 mg· kg-1 · d-1 DEHP + zinc gluconate ).At the beginning of the experiment (about 7 d before pregnancy),the female rats were administered with corresponding drugs everyday.The pregnant rats were killed and the fetal rats were removed on 19th day.The following results in each group were recorded:the body weight and the organic weight of the female rats,the number and the weight of fetus rats and the placental weight.Results The weight and the coefficient of female rats' kidney/body,spleen/body,brain/ body,and heart/body in DEHP group compared with other groups were not statistical significance (all P ＞0.05).The coefficient of female rats' liver/body,uterus/body,and ovary/body of blank group were (4.4 ± 0.7 ) ％,( 1.26 ± 0.09 ) ％,( 0.083 ± 0.009 ) ％ respectively,corn oil group were (4.5 ± 0.6 ) ％,( 1.29 ±0.10)％,(0.084 ±0.008)％,zinc group were (4.4 ±0.4)％,( 1.26 ±0.08)％,(0.084 ± 0.009 ) ％,DEHP group were ( 5.4 ± 1.0 ) ％,( 1.11 ± 0.08 ) ％,( 0.074 ± 0.012 ) ％,and DEHP + zinc group were (4.4 ± 1.0)％,( 1.28 ± 0.10)％,(0.082 ± 0.007)％ ; in DEHP group the coefficient of female rats' liver/body,uterus/body,and ovary/body compared with other groups was statistical significance ( P ＜ 0.05 ).The fetal quantity,fetal weight and placental weight of female rats of blank group were 12.8 ± 2.7,(6.03 ±0.16) g,( 1.00 ±0.03) g respectively,corn oil group were 13.6 ±3.1,(6.07 ±0.20) g,(1.00±0.04) g,zinc group were 13.3 ±3.1,(6.16 ±0.18) g,( 1.00 ±0.05) g,DEHP group were 9.2±4.1,(4.03 ±0.09) g,(0.95 ±0.03) g,and zinc +DEHP group were 12.1 ±2.9,(6.09 ± 0.17 ) g,(0.99 ±0.03 ) g.In DEHP group the fetal quantity,fetal weight and placental weight of female rats compared with other groups were statistical significance ( P ＜ 0.05 ).Conclusion DEHP can damage female rats and fetal rats in gestation period.Zinc supplied before pregnancy can relieve the influence by DEHP.

Objective To evaluate results of double eyelid operations with different incisions.Methods 100 patientswere selected randomly from out-patients that accepted double eyelid operation.Half of them were objected to double eyelid operation through a palpebral marginal incision.Incision was designed along the palpebral margin,close to boots of eyelashes.Most of the subcutaneous tissues was cut and removed,and then dermis and tarsal plate were fixed with 5 stitches of suture.About 1-3 mm wide of skin along superior margin was removed reasonably.In another 50 cases.double eyelid operation was performed with conventional incision.Incision was designed 6-8 mm far from the palpebral margin.Most of the subcutaneous tissues was removed.and then dermis and tarsal plate were fixed with 5 stitches of suture.Results Postoperative results were evaluated 5 days and 1 month after operation respectively.Postoperative double eyelid shapes were all satisfled in two groups.Swollen eyedids and scar were more inconspicuous in the cases with the palpebral-marginal incision without impairment on eyelashes.Conclusion Compared with double eyelid operation with conventional incision,blepharoplasty with palpebral margin incision has the same results but with lighter impairment,milder hydropsia and more inconspicuous scar.The key point of this procedure is the fixation of dermis and tarsal plate,keeping a good radian of double eyelid line.