BACKGROUND:The colagen and chitosan composite membrane made at a certain percentage can reduce the positive charge of chitosan, improve the adsorption force of chitosan, promote the cel adhesion and growth, migration and proliferation, and enhance the biological properties of chitosan, which has become a very excelent biological material. OBJECTIVE:To prepare chitosan-colagen composite membrane and observe its histocompatibility with corneal stroma layer. METHODS:Chitosan-colagen composite membrane was prepared. Totaly 16 New Zealand rabbits were randomly divided into experimental and control groups. The chitosan-colagen composite membrane was implanted into right eye cornea stroma bag in the experimental group, and the chitosan membrane was implanted into right eye cornea stroma bag in the control group. The slit lamp microscope, anterior segment optical coherence tomography and histological observation were conducted after transplantation. RESULTS AND CONCLUSION: Slit lamp microscope observation results showed that after 8 weeks of transplantation, degradation infiltration was visible in the center of the diaphragm, crease buckling was not obvious in the experimental group; the diaphragm was infiltrated completely and crease buckling was notable in the control group. The anterior segment optical coherence tomography results showed that at the 6thweek after transplantation, the composite membrane boundary was fuzzy, its density was very close to the normal corneal tissue, and the corneal shape recovered to normal in the experimental group. The histological findings showed that after 8 weeks of transplantation, in the experimental group, the membrane surface was degraded a little; biodegradable materials were fused with the corneal stroma; there were a smal amount of inflammatory cels infiltrated on the corneal stroma around the iris. In the control group, the membrane degradation was greater than that of the experimental group; degradable substances intertwined and fused with corneal stroma; there were more inflammatory cels infiltrated on the corneal stroma around the iris. These results demonstrate that chitosan-colagen composite membrane has degradability and good histocompatibility.

Through the study and analysis of doctor-patient relationship in the morden medical conditions in China,in this article,the author,combing with her medical praltice for many years,points out some principal problems existing in the relationship,and also puts forward some feasible countermeasures which will be helpful to set up harmonious doctor-patient relationship and ensure the durable and normal development of Chinese medical and healthy cause.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Sciatica ; therapy

BACKGROUND:Effects of pedicle screw placement on fracture reduction or stability of mechanics are influenced by various factors. Pedicle screw fixation failure is mainly due to fracture, loosening and fatigue. The main influential factors for biomechanical stability of pedicle screw are length and diameter. The research on the depth is less. OBJECTIVE:To analyze the relationship between pedicle screw placement depth of vertebral body and the fixed stability based on the biomechanics. METHODS: A model of single vertebral compression fractures was made in 15 pigs aged 5 months on L2 vertebral specimen. According to the length of anteroposterior diameter, vertebral pedicle screws were inserted in different depths (80%, 90% and 100% of anteroposterior diameter). After fixation, specimens were loaded 10 000 times at the frequency of 0.5 Hz (340±125) N on a WDT-10KN type universal material testing machine. Ranges of motion at anteflexion, backward extension, left bending and right bending and the maximum axial pulout force were measured in each group, and the difference of intergroup data was compared. RESULTS AND CONCLUSION:(1) Ranges of motion at anteflexion, backward extension, left bending and right bending in each group were significantly smaler in the 100% and 90% groups than in the 80% group (P < 0.05), and above parameters were smaler in the 100% group than in the 90% group (P< 0.05). (2) After fatigue test, the maximum axial pulout force was significantly larger in the 100% and 90% groups than in the 80% group (P< 0.05), and above data were significantly larger in the 100% group than in the 90% group (P < 0.05). (3) Results indicate that the depth into the vertebral body was significantly associated with its fixed stability. The deeper the depth into the vertebral body, the stronger the vertebral stability was: 100% group > 90% group > 80% group.

Objective To study the influence of different dosages of atorvastatin in acute coronary syndrome female patients on vascular endothelial growth factor(VEGF) and brain natriuretic peptide(BNP).Methods 60 female cases of ACS patients were divided into A and B group.30 patients in the A group were given atorvastatin 20mg per night,while the other 30 patients in the B group were taken 40mg per night.The patients were followed four weeks by evaluating serum VEGF and BNP concentration.Results After treatment for four weeks,the patients in the two groups showed significant reduction in VEGF and BNP,however,the therapeutic group showed significant difference between the two groups.Additionally,the B group further showed significant reduction in VEGF and BNP.Conclusion Using high-dose atorvastatin lowers VEGF and BNP significantly,which could reduce female cardiovascμlar events.

Objective To study the blood pressure and cardiovascular conditions in kidney transplant recipients in order to prevent the cardiovascular complications in such patients. Methods The blood pressure and ECG were monitored day and night by noninvasive means for 86 patients after transplantation.The results were analysed and compared with the pretransplantation studies. Results Hypertension occurred in 55 out of 86 patients.There was a significant difference of average blood pressure and the occurrence of abnormal ST segment or T wave between day and night whereas no significant change was observed in blood CsA concentration,blood creatinine,serum cholesterol,blood sugar,triglyceride and K+,N+,Cl-,TCO 2,Ca 2+ ,etc. Conclusions Cardiovascular complications in kidney transplant recipients is related to the high blood pressure during night,the normal day-night blood pressure rhythm being violated.It is not related to the immunosuppresives used.

Objective To study whether omeprazole induces gastric epithelial cells apoptosis and analyse the possible mechanism. Methods The cell cycle and apoptosis ratio of SGC 7901 cell line were determined by flow cytometry in the presence of omeprazole, meanwhile, TUNEL positive cells from gastric mucosa were counted after incubated with omeprazole for 72 hours. Expression of p53、 GADD 45 、 p15,16,18,19 and bcl 2 were stained by immunohistochemical assay and ATPase activity was tested. Results significant alteration with decreasing percentage of G 1 phase and increasing percentage of G 2 phase of SGC 7901 cell line could be detected after incubating 24 hours with omeprazole at double clinical doses . An apoptosis peak appeared in flow cytometry after incubating 72 hours with omeprazole. However, no genetic products such as p53, GADD 45 , p15,16,18,19 and bcl 2 were seen. Concentrations of ATPase were markedly increased from 0.326 0.387 before incubated with omeprazole to 0.054 0.103 after incubated with omeprazole. TUNEL positive reaction cell counts in fresh mucosa was also increased after incubated with omeprazole for 72 hours( P

0 05). It is suggested that: ①When foot brace fixed the femur at inward rotation of 0?, 15?and then 15? after moving body position, and ROI was near the neck, BMDs of the neck and the trochanter were not affected in either of the three positions, with femoral neck with highest BMD; ②When BMD value of the femoral Ward′s triangle is taken as the index, it is better to take the mean of 3～5 scannings at one position.

Objective To explore the correlation among serum immunoglobulin G4 (IgG4),transforming growth factor-β1 (TGF-β1),connective tissue growth factor (CTGF) and Hashimoto thyroiditis (HT) thyroid fibrosis.Methods Case-control study.A total of 159 patients with HT visiting the Wuhan Union Hospital were collected from May 2013 to March 2015.All patients were divided into IgG4 HT group (IgG4≥1.35 g/L,n =39) and non-IgG4 HT group (IgG4 ＜ 1.35 g/L,n =120).The serum IgG4,TGF-β1 and CTGF were determined by enzyme-linked immunosorbent assays.The levels of serum thyroid peroxidase antibodies (TPOAb) and thyroglobulin antibodies (TgAb) were measured by electrochemiluminescence immunoassay.Meanwhile,ultrasound of the thyroid gland was performed.Statistical analysis was performed by use of SPSS 17.0 software.The Mann-Whitney U test was used to compare two independent samples of non-normal distribution data,Fisher's exact test was employed to analyze thyroid imaging differences,correlation test was performed to examine various correlations,multivariate Logistic regression analysis was used to evaluate thyroid fibrosis risk factors.Results Compared with that of non-IgG4 HT group,IgG4 HT group:TPOAb [(455.2 ± 169.7) vs.(186.5 ± 102.3),U =27.0,P=0.003],TgAb [(984.6±452.7) vs.(289.3 ±245.1),U=30.5,P=0.017],TGF-β1 [(1.45±0.97) vs.(0.30±0.22),U=119.0,P=0.035] andCTGF [(88.65±14.39) vs.(62.21± 8.76),U =69.0,P =0.039] were significantly higher,thyroid ultrasound showed obvious fibrosis (35/4 vs.32/88,x2 =48.03,P =0.000);significant positive linear correlation between IgG4 and TPOAb (r =0.719,P =0.000),CTGF and TGF-β1 (r =0.500,P ＜ 0.01) respectively.Logistic regression analysis indicated that all the serum IgG4,TPOAb,TGF-β1 and CTGF were independent risk factors of thyroid fibrosis [IgG4,odds ratio (OR) =1.968,P =0.014,95％ confidence interval (CI) =1.287-2.041;TPOAb,OR =2.537,P =0.012,CI =1.322-2.869;TGF-β1,OR =1.549,P =0.023,CI =1.105-1.498;CTGF,OR =1.185,P =0.046,CI =1.204-1.625].Conclusion The highlevel of circulating antibodies,IgG4,TGF-β1 and CTGF were significantly associated with thyroid fibrosis,and were independent risk factors of HT fibrosis.

Objective To know the situation of formaldehyde pollution in the indoor air and the influencing factors in Shandong University. Methods PPM400 formaldehyde analyzer adjusted with spectrophotometry was used to determine the concentration of formaldehyde in the indoor air in the rooms which were decorated in different levels in the campus of Shandong University. Results The average concentration of formaldehyde in the indoor air was 0.05 mg/m3(median), in the outdoor air it was below the detection limit. The qualified rate of formaldehyde concentration in indoor air was 79.6%. The average concentration of formaldehyde in indoor air was(0.045?0.003 )mg/m3 with the windows open and it was (0.212?0.079)mg/m3 with the windows closed. Conclusion The indoor air of Shandong University is polluted with formaldehyde to some extent. Fitment and use of veneers, flakeboard are the main pollutant source. The level of formaldehyde pollution is higher in the newly or nicely decorated rooms and in bad ventilation.